Fig. 5
CFU counts in 24 h old fourth instars (Larvae), 6–12 h adult females, and 72 h adult females. Treatments and the number of individuals analyzed per life stage are the same as in Fig. 4. For A. aegypti, bacterial loads significantly differed among treatments in larvae (ANOVA: F (5,18) = 13.9, P < 0.0001) and 6–12 h adults (ANOVA: F (5,22) = 19.6, P < 0.0001), but did not differ in 72 h adults (ANOVA: F (5,16) = 1.6, P = 0.21). Between stage comparisons indicated that bacterial loads significantly differed between larvae, 6–12 h adults and 72 h adults (ANOVA: F (2,71) = 31.6, P < 0.0001; followed by a Tukey-Kramer HSD test). For A. atropalpus, bacterial loads significantly differed among treatments in larvae (ANOVA: F (5,34) = 12.4, P < 0.0001), 6–12 h adults (ANOVA: F (5,33) = 8.9, P < 0.0001) and 72 h adults (ANOVA: F (5,36) = 6.8, P = 0.0002). Between stage comparisons indicated that larvae (*) had higher bacterial loads than 6–12 h or 72 h adults, which did not differ from one another (NS) (ANOVA: F (1,118) = 86.6, P < 0.0005; followed by a Tukey-Kramer HSD test). For A. aegypti adults (6–12 h) and A. atropalpus adults (6–12 and 72 h), Dunnett’s tests indicated treatment differences were due to higher colony counts for gnotobiotic individuals colonized by Chryseobacterium (red data points)