Gut bacteria differentially affect egg production in the anautogenous mosquito Aedes aegypti and facultatively autogenous mosquito Aedes atropalpus (Diptera: Culicidae)
© The Author(s). 2016
Received: 2 May 2016
Accepted: 21 June 2016
Published: 30 June 2016
Aedes aegypti and A. atropalpus are related mosquitoes that differ reproductively. Aedes aegypti must blood-feed to produce eggs (anautogenous) while A. atropalpus always produces a first clutch of eggs without blood-feeding (facultatively autogenous). We recently characterized the gut microbiota of A. aegypti and A. atropalpus that were reared identically in the laboratory. Here, we assessed the effects of specific members of the gut microbiota in A. aegypti and A. atropalpus on female fitness including egg production.
Gnotobiotic A. aegypti and A. atropalpus larvae were colonized by specific members of the gut microbiota. Survival, development time, size and egg production for each treatment was then compared to axenic and conventionally reared larvae.
Most species of bacteria we tested supported normal development and egg production by A. aegypti but only one betaproteobacterium, a Comamonas, supported development and egg production by A. atropalpus to equivalent levels as conventionally reared females. Aedes atropalpus females colonized by Comamonas contained similar stores of glycogen and protein as conventionally reared females, whereas females colonized by Aquitalea did not. Small differences in bacterial loads were detected between gnotobiotic and conventionally reared A. aegypti and A. atropalpus, but this variation did not correlate with the beneficial effects of Comamonas in A. atropalpus.
Specific members of the gut microbiota more strongly affected survival, size and egg production by A. atropalpus than A. aegypti.
KeywordsMicrobiota Development Reproduction Oogenesis Clutch size
Most mosquito species are anautogenous, which means that after mating with males, adult females must consume at least one blood meal from a vertebrate host for each clutch of eggs they produce and lay . Repeated cycles of blood-feeding and egg production also underlie why several mosquito species have evolved into vectors that transmit pathogens that cause several serious diseases in humans and other animals. In contrast, some mosquito species are autogenous and produce eggs without blood-feeding . Facultatively autogenous species emerge as adults and lay a first clutch of eggs without blood-feeding but must blood-feed to produce additional clutches. Obligately autogenous species never blood-feed.
Regulation of egg formation is best understood in the anautogenous mosquito Aedes aegypti, which vectors several human pathogens including the viruses that cause yellow fever, dengue fever and Zika virus disease. In brief, the fat body of newly emerged A. aegypti females becomes competent to produce yolk proteins through the activity of juvenile hormone [2, 3]. However, oogenesis remains arrested until a blood meal is taken, which triggers the release of two neurohormones from the brain, insulin-like peptides (ILPs) and ovary ecdysteroidogenic hormone (OEH), that stimulate the ovaries to produce ecdysone [4, 5]. Ecdysone, ILPs and other factors regulate the fat body to produce yolk proteins that are packaged into primary oocytes to produce mature eggs [4–8]. Nutrients in the blood meal together with teneral reserves acquired during larval feeding provide the resources needed to produce yolk proteins [9–11].
The rockpool mosquito, Aedes atropalpus, is closely related to A. aegypti  but is facultatively autogenous. Aedes atropalpus females produce similar numbers of eggs in the first gonadotropic cycle as A. aegypti, but fully rely on nutrient reserves acquired from feeding during the larval stage to do so [10, 13–15]. ILPs and OEH play a similar role in stimulating the ovaries in A. atropalpus to produce ecdysone and the fat body to synthesize yolk proteins as determined for A. aegypti [15, 16]. What differs is that A. atropalpus females release these neurohormones shortly after emerging as adults rather than in response to blood-feeding [15, 16]. The mechanism(s) that triggers blood meal-independent release of these hormones remains unclear, although data suggest it is linked to nutrient sensing and the availability of sufficient teneral reserves to support egg production [10, 15, 16].
Microorganisms in the digestive tract can also influence nutrient acquisition by animals including insects . Mosquitoes host low diversity bacterial communities in their digestive tract that consist primarily of gram-negative aerobes and facultative anaerobes [18–25]. Mosquitoes acquire most if not all of these bacteria from the aquatic habitat they develop in as larvae while transmitting some but not all community members to adults [19, 21].
We recently used 16S rDNA sequencing to characterize the gut microbiota of A. aegypti and A. atropalpus larvae reared under identical environmental conditions in the laboratory. Results showed that similar communities of bacteria were present in the aquatic habitat of both species during larval development. However, Bacteroidetes (Flavobacteriaceae) and Actinobacteria (Microbacteriaceae) dominated the gut community in A. aegypti larvae, whereas Proteobacteria (Class Betaproteobacteria) dominated the community in A. atropalpus . We developed methods to produce axenic (i.e. bacteria-free) larvae, which showed that both species fail to develop beyond the first instar in the absence of gut bacteria, but develop normally into adults if recolonized by bacteria in their laboratory aquatic habitat. We also developed methods to produce gnotobiotic larvae that were colonized by a particular species of bacterium. Bioassays showed that several species of bacteria present in the laboratory aquatic habitat could individually colonize axenic A. aegypti larvae, and that the resulting gnotobiotic larvae also develop normally into adults . However, no studies have examined the effects of the gut microbiota in larvae on egg production by A. aegypti or any fitness traits in A. atropalpus.
In this study, we produced gnotobiotic A. aegypti and A. atropalpus larvae that were singly colonized by abundant members of the larval gut microbiota and compared their effects on female-related fitness traits including egg production in the first ovarian cycle. Our results showed that gnotobiotic A. aegypti singly colonized by several members of the gut microbiota developed and produced eggs similarly to conventionally reared A. aegypti females with a mixed community of bacteria. In contrast, only one community member we tested rescued development and egg production to equivalent levels as conventionally reared A. atropalpus females.
Conventional rearing of A. aegypti and A. atropalpus
The UGAL strain of A. aegypti and Bass Rock strain of A. atropalpus were conventionally reared in the same insectary at 27 °C, 60 % relative humidity, and 16 h light: 8 h dark photoperiod . Larvae were fed a standard diet consisting of finely ground rat chow (Purina): lactalbumin: brewers yeast (1:1:1) in open aluminum rearing pans containing distilled water . Pupae were transferred from larval rearing pans to plastic cages for adult emergence. These methods produced large cohorts of adult A. aegypti and A. atropalpus that were similar in size. Adults were fed 10 % sucrose in water (wt/vol) ad libitum. Adult female A. aegypti were blood-fed two days post-emergence on an anesthetized rat until engorged. Aedes aegypti females laid a clutch of eggs approximately 36 h after blood-feeding on damp filter paper. Aedes atropalpus females in contrast laid clutches of eggs on damp filter paper 3–5 days post-emergence.
Isolation and taxonomic assignment of bacteria from larvae
Bacterial isolates were maintained as summarized in Additional file 1: Table S1. Bacteria were isolated from conventionally reared A. aegypti and A. atropalpus larvae by collecting and surface sterilizing fourth instars followed by homogenization in 1.6 ml centrifuge tubes in sterile phosphate buffered saline (PBS) using a plastic pestle . Homogenates were then plated on Luria broth (LB), brain-heart infusion (BHI), tryptic soy agar (TSA), Reasoner’s 2A (R2A), or blood agar plates at 27 °C for 24–72 h (Additional file 1: Table S1). Single colonies were picked for serial dilution streaking followed by DNA isolation from a single colony using the Gentra Puregene Yeast/Bact kit (Qiagen). After PCR amplification using universal primers (1492F and 129R in Additional file 1: Table S2) and cloning into a vector (pCR 2.1 TOPO TA cloning, Invitrogen), ~1,000 bp of the 16S–23S internal transcribed spacer (ITS) region was Sanger sequenced. Resulting sequence data were then used to design genus-specific primers for each isolate of interest (Additional file 1: Table S2). Specificity of primers for a given isolate was verified by PCR using DNA from each of the identified bacterial isolates as previously described .
Inoculation of axenic first instars with particular gut community members
Axenic larvae were produced by surface sterilizing eggs from the conventionally reared A. aegypti and A. atropalpus colonies followed by hatching of first instars in sterile water per Coon et al. . Larval diet was sterilized by exposure to 5 kGy from a cobalt 60 gamma radiation source, while 10 % sucrose was filtered sterilized for feeding adults. First instars were inoculated with a particular species of bacterium by placing 20 axenic larvae in a 150 × 15 mm Petri dish containing water sterilized by autoclaving followed by addition of sterile diet and approximately 108 cells of a given bacterial isolate suspended in sterile water. Sterilized diet was thereafter added to dishes every other day until death or pupation. Pupae were surface-sterilized by placing in 2 % (vol/vol) bleach for 2 min and rinsing 3 times in sterile water. Pupae were then placed in sterile water in an autoclaved polypropylene plastic chamber (Olcott Plastics) for adult emergence. The axenic status of larvae and food were confirmed by culture-based methods and PCR analysis using universal 16S rRNA primers (27F and 1492R in Additional file 1: Table S2) . The same methods were also used to determine the presence of bacteria or a particular isolate in water, larvae or adults.
Larval survival and development time assays
Dishes containing 20 first instars inoculated with a particular bacterial isolate were produced as described above. Dishes containing 20 axenic first instars with sterile food but no bacteria served as a negative control, while 20 conventional first instars in non-sterile water but fed sterile food served as the positive control. Dishes were then maintained under the same environmental conditions. The number of larvae that pupated per dish for each treatment was recorded daily. All pupae from a dish were then removed, surface sterilized, and placed in sterile water in sterile containers as described above. Dead larvae and pupae were removed and discarded. Survival to adulthood was measured as the proportion of first instars that successfully emerged as adults. Between 5 and 30 replicate dishes were monitored for each treatment. With 20 larvae per replicate, we overall monitored from 100 to 600 first instars per treatment.
Adult size and mature egg production assays
Gnotobiotic and conventionally reared adult females were transferred to polypropylene cages (Olcott Plastics) lined with moist filter paper and containing a cotton wick soaked with water. Filter paper, wicks, water and cages were autoclaved before use. The number of eggs laid by each female was counted by visually inspecting the filter paper using a dissecting microscope. Each female was then dissected in PBS and the number of mature eggs remaining in the ovaries was also determined by visual inspection using previously established criteria . These criteria included that unlaid mature eggs had to be ≥ 300 μm in length and have a fully formed chorion. Egg production was quantified for individual, mated A. atropalpus females at 120 h post-emergence. Egg production was quantified for individual, mated A. aegypti females 72 h after blood-feeding on a surface-sterilized rat. First clutch size of each female was defined as the total number of oviposited plus mature eggs remaining in the ovaries. The size of each ovipositing female was then determined by measuring the length of the forewing from the axillary incision to the tip excluding fringe using an ocular micrometer. A minimum of 20 females per treatment was assayed.
Nutrient reserves in conventional and gnotobiotic A. atropalpus
Conventionally reared and gnotobiotic adult females were collected 12 h post-emergence followed by dissection in PBS and removal of the digestive tract. The remaining body wall, which contains the fat body, from two individuals was transferred to a microfuge tube and homogenized in 100 μl of water (protein assay) or 100 μl of Na2SO4 with 200 μl of methanol (glycogen, lipid assays) followed by storage at −80 °C before use . After centrifugation (12,000× g, 4 °C), supernatants were used to determine total protein, glycogen and lipid amounts as previously described [4, 16]. A total of 10 body wall pairs were assayed for each treatment.
Bacterial load in conventional and gnotobiotic A. aegypti and A. atropalpus
The number of bacteria in gnotobiotic larvae and adults was assessed by standard plate counts. Individual larvae were collected after molting to the fourth instar from replicate dishes, surface-sterilized, homogenized in 100 μl of PBS as described above, serially diluted and plated on LB agar. Colony forming units (CFUs) were then determined after incubation of plates at 27 °C for 24 h. Homogenates were also plated from adult females collected 6–12 h and either 24 h post-blood meal (A. aegypti) or 72 h post-emergence (A. atropalpus). At least 4 larvae or adults were analyzed per treatment with each sample internally replicated 4 times.
All analyses were performed using R (http://www.r-project.org/). Development time and total nutrient amounts were tested for normality and equality of variances before analysis by one-way analysis of variance (ANOVA), followed by post-hoc comparisons using Dunnett’s tests. Survival data were analyzed by Chi-square or Fisher’s exact tests followed by post-hoc Bonferroni-corrected pairwise tests to compare treatments. Bacterial counts were analyzed by ANOVA followed by either post-hoc comparison to the conventional positive control using Dunnett’s test or Tukey-Kramer Honest Significant Difference (HSD) tests. The number of mature eggs females produced was analyzed by Fisher’s exact test to compare the proportion of females that produced one or more mature eggs in each bacterial treatment while the effects of size were analyzed by logistic regression. These data were also examined by an analysis of covariance (ANCOVA) followed by post-hoc comparisons using a Dunnett’s test.
Individual bacteria differentially affect A. aegypti and A. atropalpus development
We first compared development time to pupation and survival to adulthood of gnotobiotic larvae colonized by individual bacterial isolates to that of conventionally reared control larvae. We focused our assays on representative genera from each of the major bacterial phyla previously identified from the digestive tracts of our laboratory mosquitoes (; Additional file 1: Table S1): Paenibacillus (Firmicutes: Paenibacillaceae), Chryseobacterium (Bacteroidetes: Flavobacteriaceae), Sphingobacterium (Bacteroidetes: Sphingobacteriaceae), Microbacterium (Actinobacteria: Microbacteriaceae), Leucobacter (Actinobacteria: Microbacteriaceae), Aquitalea (Proteobacteria: Neisseriaceae) and Comamonas (Proteobacteria: Comamonadaceae). Two of these genera, Aquitalea and Comamonas, belong to the Betaproteobacteria and were previously identified as dominant members of the A. atropalpus larval gut community by pyrosequencing. All other genera were previously isolated from A. aegypti (; Additional file 1: Table S1). Members of the Bacteroidetes (Flavobacteriaceae, Sphingobacteriaceae) and Actinobacteria (Microbacteriaceae) are commonly found in anautogenous species both in the lab and field [18–25], and make up as much as 90 % of the gut bacterial community in our laboratory mosquito cultures .
We collected eggs from conventionally reared A. aegypti and A. atropalpus and produced axenic (i.e. bacteria-free) first instars, which were either maintained in sterile water with sterile food or placed into water inoculated with one of the above isolates plus food. PCR screening of bacterial 16S rDNA using universal primers 6 h after hatching confirmed the presence of bacteria in conventionally reared first instars and the absence of bacteria in axenic larvae (Additional file 1: Figure S1). Screening with genus specific primers also confirmed that inoculation of axenic larvae with the above isolates resulted in larvae that contained each bacterium at 6 h post-inoculation (Additional file 1: Figure S1).
For the individuals in each treatment that developed into adults, we estimated female size by measuring forewing length, which has been used in several other studies of mosquitoes including A. aegypti and A. atropalpus to estimate body size [10, 28, 29]. There were no differences in size between conventionally reared A. aegypti adult females and adults that emerged from larvae colonized by Paenibacillus, Chryseobacterium, Sphingobacterium, Aquitalea or Comamonas (Fig. 1c). However, only gnotobiotic A. atropalpus larvae inoculated with Aquitalea or Comamonas developed into adults that did not significantly differ in size from conventionally reared A. atropalpus (Fig. 1c). Aedes atropalpus adults produced from larvae inoculated with Paenibacillus, Chryseobacterium or Sphingobacterium were significantly smaller (Fig. 1c).
Several bacteria support normal egg production in A. aegypti but not A. atropalpus
We next focused on those species of bacteria that supported development of at least some A. aegypti and A. atropalpus to adulthood by assessing their effects on egg production by females. For each treatment, females were mated with males from the same cohort and subsequently housed individually in sterile containers for 36–120 h. Aedes aegypti females were blood-fed 2 days post emergence. Egg production data were recorded in three ways: (i) the proportion of females for each treatment that laid or contained at least one mature egg; (ii) the total number of mature eggs per female (laid plus present in the ovaries); and (iii) the total number of laid eggs.
Bacterial loads in A. aegypti and A. atropalpus are similar
Aedes aegypti and A. atropalpus females are similar in size when reared as larvae under the same conditions, but A. aegypti emerges with lower nutrient reserves as measured by stored glycogen and protein [10, 15]. For both species, results in the literature also indicate the nutrients consumed by larvae affect adult size and the average number of eggs per clutch females lay [10, 30]. This finding suggests nutrient based thresholds play a role in egg production [10, 13, 15, 16]. In effect, A. aegypti females emerge with insufficient nutrient reserves and enter an arrested previtellogenic state that blood-feeding overcomes by providing additional nutrients. Aedes atropalpus females in contrast emerge with sufficient nutrients to initiate egg formation without blood-feeding. Enhanced nutrient acquisition along with genetic factors has also been implicated in anautogenous versus autogenous reproduction in other species [14, 31–35].
An earlier study used antibiotic treatment to determine whether gut bacteria in adult A. aegypti affect blood meal digestion . Treatment with carbenicillin and tetracycline reduced the abundance of culturable bacteria in the midgut, which correlated with slower breakdown of the blood meal bolus and small, but statistically significant reductions in the number of eggs females laid. Identification of an Enterobacter and Serratia species with hemolytic activity further supported a role for the gut microbiota in blood meal processing. Outside of this work, however, what role if any the gut microbiota plays in female-associated fitness including egg production is unknown in mosquitoes , which is why we conducted this study.
Our previous results showed that certain genera of Actinobacteria (Microbacterium and Leucobacter), Bacteroidetes (Chryseobacterium and Sphingobacterium), Betaproteobacteria (Comamonas and Aquitalea) and Firmicutes (Paenibacillus) are present in the digestive tracts of both A. aegypti and A. atropalpus larvae when reared under identical conditions in the laboratory . The relative abundance of these community members, however, differs with Bacteroidetes and Actinobacteria predominating in A. aegypti and Betaproteobacteria predominating in A. atropalpus. These differences guided our choice of the gut bacteria used in this study to compare the effects individual members have on fitness relative to conventionally reared individuals with a mixed community of bacteria or axenic larvae with no gut bacteria.
The first part of our study corroborates prior findings by showing that axenic A. aegypti and A. atropalpus larvae die as first instars. They also show that Microbacterium and Leucobacter fail to colonize larvae of either species, which likewise results in no survival. This finding strongly suggests Microbacterium and Leucobacter present in our conventionally reared larvae require other bacteria to survive in A. aegypti and A. atropalpus. The other community members we tested resulted in nearly all A. aegypti larvae developing into adults that did not differ from adults of conventionally reared larvae in development time, size or the number of mature eggs females produced after consuming a blood meal. In contrast, only the two Betaproteobacteria (Aquitalea and Comamonas) tested in A. atropalpus supported survival, development times and adult body sizes that were similar to those of conventionally reared larvae. Only A. atropalpus larvae colonized by Comamonas produced the same number of eggs per first clutch as conventionally reared females. The three other community members we tested (Paenibacillus, Chryseobacterium and Sphingobacterium) are more abundant in conventionally reared A. aegypti than A. atropalpus. Each of these Paenibacillus, Chryseobacterium and Sphingobacterium species colonized A. atropalpus to produce gnotobiotic larvae and increased survival relative to axenic larvae. However, survival and other fitness measures were also much lower than those of conventionally reared A. atropalpus.
Taken together, these results indicate that several members of the gut community in A. aegypti larvae support development and egg production to levels that are comparable to conventionally reared individuals with a mixed bacterial community. They also suggest the differential abundance of these bacteria in conventionally reared A. aegypti larvae does not reflect the ability of these community members to rescue larval development or egg production by adult females after a blood meal. This is not the case though for A. atropalpus, where the two Betaproteobacteria we tested are more abundant in conventionally reared larvae and also had a more positive effect on survival, size and egg production in gnotobiotic larvae. Thus, one important element of autogeny in A. atropalpus could be greater dependence on the composition of the gut microbiota for development and reproduction. In addition, the impacts of gut bacteria on nutrient reserves in A. aegypti are potentially compensated for by the extra nutrients females obtain from a blood meal.
Gnotobiotic A. atropalpus colonized by Comamonas emerged with similar stores of glycogen and protein but significantly lower total lipid than conventionally reared females. In contrast, gnotobiotic females colonized by Aquitalea emerged with lower glycogen, protein, and lipid stores. Reduced nutrient uptake and metabolism in axenic or gnotobiotic backgrounds have been reported for mice, zebrafish, Daphnia and Drosophila [38–42]. The gut microbiota of Drosophila is also known to promote growth by modulating nutrient sensing through the target of rapamycin and insulin signaling pathways, which intersect to regulate metabolism [43, 44]. Thus, the higher lipid stores in conventionally reared females suggest other community members besides Aquitalea or Comamonas are required for normal lipid storage. However, lower lipid levels in Comamonas-colonized females did not correlate with reduced first clutch sizes, which suggests stored protein is the more important determinant for production of a first clutch. The importance of stored protein may also underlie why gnotobiotic females colonized by Aquitalea produced smaller clutches.
Most insects including mosquitoes lay eggs that by dry weight consist primarily of protein but also 30–40 % lipid that is predominantly triacylglycerol (TAG) [9, 45]. TAG is synthesized from free fatty acids and glycerol but insect eggs exhibit little or no de novo fatty acid synthetic activity. Thus, yolk protein and TAG are both imported from the fat body into oocytes during oogenesis . In A. aegypti, digestion of a blood meal primarily provides amino acids, which suggests TAG packaged into eggs during the first gonadotropic cycle derives primarily from stores in the fat body while yolk protein is produced from a combination of nutrient stores and blood meal derived amino acids . In the case of A. atropalpus, however, TAG and yolk protein must both come from teneral reserves in the fat body. The mobilization of nutrient stores in A. atropalpus colonized by Comamonas is of interest because these females have lower stored lipid relative to females of conventionally reared larvae, but the number of mature eggs produced by both sets of females was similar, as were their protein and carbohydrate stores. How these gnotobiotic females accomplish this is unclear. One possibility is their lower lipid reserves are sufficient to mobilize as TAG and produce a comparable number of mature eggs as conventionally reared females. Alternatively, excess carbohydrate or protein stores may be catabolized to acetyl-CoA, which is the precursor for fatty acid synthesis and thus TAG importation, and would allow females to produce a similar number of mature eggs as conventionally reared females with larger lipid reserves.
Excluding Microbacterium and Leucobacter, our measures of bacterial abundance in gnotobiotic A. aegypti and A. atropalpus showed that the average number of living bacteria per individual was either similar or slightly lower in most gnotobiotic treatments when compared to conventionally reared controls. This finding indicates each of the community members we tested proliferated in the absence of other community members, but usually did not exceed the total abundance of bacteria in conventional larvae that host a mixed bacterial community. These results indicate that the higher survival and egg production of gnotobiotic A. atropalpus colonized by Comamonas was not due to this bacterium being more abundant in the guts of larvae or adults than the other community members we tested. Thus, other features of this bacterium likely underlie its positive effects for A. atropalpus. These data also indicate that bacterial abundance in gnotobiotic and conventionally reared individuals is lower in adults than fourth instar larvae, which is a feature previously noted for A. aegypti .
Results of this study show that A. aegypti and A. atropalpus larvae both require gut bacteria to develop into adults, but individual gut bacteria differentially affect development and reproduction by both species when reared as gnotobiotic larvae. In particular, autogenous egg production by A. atropalpus has a greater dependence on the specific members of the gut microbiota, while the added nutrients females obtain from blood-feeding potentially make A. aegypti females less dependent on the composition of the gut microbiota.
CFU, Colony forming unit; ILPs, Insulin-like peptides; OEH, Ovary ecdysteroidogenic hormone
The authors thank A. Elliot for assistance with the fecundity and teneral reserve assays. We also thank both A. Elliot and S.R. Robertson for assistance with mosquito colonies.
This work was supported by awards from the US National Science Foundation (Graduate Research Fellowship 038550–04) (KLC) and National Institutes of Health (R01AI06892 (MRS), University of Georgia genetics training grant T32GM007103).
Availability of data and materials
Essential data are presented in the main text of the paper. Nucleotide sequence data reporting the gut bacteria community members of the mosquitoes used in the study were assigned GenBank accession nos. KJ192338–KJ192343.
KLC participated in conception of the study, data acquisition, data analysis and writing of the manuscript. MRB participated in conception of the study and writing of the manuscript. MRS participated in conception of the study, data analysis, and writing of the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Ethics approval and consent to participate
The University of Georgia Animal Use Protocol (A2014 12-013-A1) to blood-feed mosquitoes was approved by the University of Georgia Institutional Animal Care and Use Committee, which oversees and provides veterinary care, maintains an Assurance of Compliance with the US Public Health Service and is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International and licensed by the US Department of Agriculture. No human participants, human data or human tissue was used in the study.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Clements AN. The biology of mosquitoes, Vol. 1. Development, nutrition, and reproduction. New York: Chapman and Hall; 1992.Google Scholar
- Zhu J, Chen L, Raikhel AS. Posttranscriptional control of the competence factor betaFTZ-F1 by juvenile hormone in the mosquito Aedes aegypti. Proc Natl Acad Sci U S A. 2003;100:13338–43.View ArticlePubMedPubMed CentralGoogle Scholar
- Noriega FG. Nutritional regulation of JH synthesis: a mechanism to control reproductive maturation in mosquitoes? Insect Biochem Mol Biol. 2004;34:687–93.View ArticlePubMedGoogle Scholar
- Brown MR, Clark KD, Gulia M, Zhao Z, Garczynski SF, Crim JW, et al. An insulin-like peptide regulates egg maturation and metabolism in the mosquito Aedes aegypti. Proc Natl Acad Sci U S A. 2008;105:5716–21.View ArticlePubMedPubMed CentralGoogle Scholar
- Vogel KJ, Brown MR, Strand MR. Ovary ecdysteroidogenic hormone requires a receptor tyrosine kinase to activate egg formation in the mosquito Aedes aegypti. Proc Natl Acad Sci U S A. 2015;112:5057–62.View ArticlePubMedPubMed CentralGoogle Scholar
- Wu Q, Brown MR. Signaling and function of insulin-like peptides in insects. Annu Rev Entomol. 2006;51:1–24.View ArticlePubMedGoogle Scholar
- Riehle MA, Brown MR. Insulin stimulates ecdysteroid production through a conserved signaling cascade in the mosquito Aedes aegypti. Insect Biochem Mol Biol. 1999;29:855–60.View ArticlePubMedGoogle Scholar
- Gulia-Nuss M, Robertson AE, Brown MR, Strand MR. Insulin-like peptides and the target of rapamycin pathway coordinately regulate blood digestion and egg maturation in the mosquito Aedes aegypti. PLoS One. 2011;6(5):e20401.View ArticlePubMedPubMed CentralGoogle Scholar
- Attardo GM, Hansen IA, Raikhel AS. Nutritional regulation of vitellogenesis in mosquitoes: implications for anautogeny. Insect Biochem Mol Biol. 2005;35:661–75.View ArticlePubMedGoogle Scholar
- Telang A, Li Y, Noriega FG, Brown MR. Effects of larval nutrition on the endocrinology of mosquito egg development. J Exp Biol. 2006;209:645–55.View ArticlePubMedGoogle Scholar
- Brandon MC, Pennington JE, Isoe J, Zamora J, Schillinger AS, Miesfeld RL. TOR signaling is required for amino acid stimulation of early trypsin protein synthesis in the midgut of Aedes aegypti mosquitoes. Insect Biochem Mol Biol. 2008;38:916–22.View ArticlePubMedPubMed CentralGoogle Scholar
- Wilkerson RC, Linton Y-M, Fonseca DM, Schultz TR, Price DC, Strickman DA. Making mosquito taxonomy useful: a stable classification of tribe Aedini that balances utility with current knowledge of evolutionary relationships. PLoS One. 2015;10(7):e0133602.View ArticlePubMedPubMed CentralGoogle Scholar
- Chambers GM, Klowden MJ. Nutritional reserves of autogenous and anautogenous selected strains of Aedes albopictus (Diptera: Culicidae). J Med Entomol. 1994;31:554–60.View ArticlePubMedGoogle Scholar
- Su T, Mulla MS. Nutritional reserves, body weight, and starvation tolerance of autogenous and anautogenous strains of Culex tarsalis (Diptera: Culicidae). J Med Entomol. 1997;34:68–73.View ArticlePubMedGoogle Scholar
- Gulia-Nuss M, Elliot A, Brown MR, Strand MR. Multiple factors contribute to anautogenous reproduction by the mosquito Aedes aegypti. J Insect Physiol. 2015;82:8–16.View ArticlePubMedGoogle Scholar
- Gulia-Nuss M, Eum JH, Strand MR, Brown MR. Ovary ecdysteroidogenic hormone activates egg maturation in the mosquito Georgecraigius atropalpus after adult eclosion or a blood meal. J Exp Biol. 2012;215:3758–67.View ArticlePubMedPubMed CentralGoogle Scholar
- Engel P, Moran NA. The gut microbiota of insects- diversity in structure and function. FEMS Microbiol Rev. 2013;37:699–735.View ArticlePubMedGoogle Scholar
- Wang Y, Gilbreath TM, Kukutla P, Yan GY, Xu JN. Dynamic gut microbiome across life history of the malaria mosquito Anopheles gambiae in Kenya. PLoS ONE. 2011;6(9):e24767.View ArticlePubMedPubMed CentralGoogle Scholar
- Boissiere A, Tchioffo MT, Bachar D, Abate L, Marie A, Nsango SE, et al. Midgut microbiota of the malaria mosquito vector Anopheles gambiae and interactions with Plasmodium falciparum infection. PLoS Pathog. 2012;8(5):e1002742.View ArticlePubMedPubMed CentralGoogle Scholar
- Osei-Poku J, Mbogo CM, Palmer WJ, Jiggins FM. Deep sequencing reveals extensive variation in the gut microbiota of wild mosquitoes from Kenya. Mol Ecol. 2012;21:5138–50.View ArticlePubMedGoogle Scholar
- Coon KL, Vogel KJ, Brown MR, Strand MR. Mosquitoes rely on their gut microbiota for development. Mol Ecol. 2014;23:2727–39.View ArticlePubMedPubMed CentralGoogle Scholar
- Gimonneau G, Tchioffo MT, Abate L, Boissiere A, Awono-Ambene PH, Nsango SE, et al. Composition of Anopheles coluzzii and Anopheles gambiae microbiota from larval to adult stages. Infect Genet Evol. 2014;28:715–24.View ArticlePubMedGoogle Scholar
- Minard G, Tran G, Dubost A, Tran-Van V, Mavingui P, Moro CV. Pyrosequencing 16S rRNA genes of bacteria associated with wild tiger mosquito Aedes albopictus: a pilot study. Front Cell Infect Microbiol. 2014;4:59.View ArticlePubMedPubMed CentralGoogle Scholar
- Duguma D, Hall MW, Rugman-Jones P, Stouthamer R, Terenius O, Neufeld JD, Walton WE. Developmental succession of the microbiome of Culex mosquitoes. BMC Microbiol. 2015;15:140.View ArticlePubMedPubMed CentralGoogle Scholar
- Muturi EJ, Kim C, Bara J, Bach EM, Siddappaji MH. Culex pipiens and Culex restuans mosquitoes harbor distinct microbiota dominated by few bacterial taxa. Parasit Vectors. 2016;9:18.View ArticlePubMedPubMed CentralGoogle Scholar
- Riehle MA, Brown MR. Insulin receptor expression during development and a reproductive cycle in the ovary of the mosquito Aedes aegypti. Cell Tissue Res. 2002;308:409–20.View ArticlePubMedGoogle Scholar
- Zhou G, Flowers M, Friedrich K, Horton J, Pennington J, Wells MA. Metabolic fate of [14C]-labeled meal protein amino acids in Aedes aegypti mosquitoes. J Insect Physiol. 2004;50:337–49.View ArticlePubMedGoogle Scholar
- Van Handel E, Day JF. Correlation between wing length and protein content of mosquitoes. J Am Mosq Control Assoc. 1989;5:180–2.PubMedGoogle Scholar
- Nasci R. Relationship of wing length to adult dry weight in several mosquito species (Diptera: Culicidae). J Med Entomol. 1990;27:716–9.View ArticlePubMedGoogle Scholar
- Briegel H. Physiological bases of mosquito ecology. J Vector Ecol. 2003;28:1–11.PubMedGoogle Scholar
- O’Meara GF. Gonotrophic interactions in mosquitoes - kicking the blood-feeding habit. Flor Entomol. 1985;68:122–33.View ArticleGoogle Scholar
- Merritt RW, Dadd RH, Walker ED. Feeding behavior, natural food, and nutritional relationships of larval mosquitoes. Annu Rev Entomol. 1992;37:349–76.View ArticlePubMedGoogle Scholar
- Hugo LE, Kay BH, Ryan PA. Autogeny in Ochlerotatus vigilax (Diptera: Culicidae) from southeast Queensland. Austr J Med Entomol. 2003;40:897–902.View ArticleGoogle Scholar
- Mori A, Romero-Severson J, Black IV WC, Severson DW. Quantitative trait loci determining autogeny and body size in the Asian tiger mosquito (Aedes albopictus). Heredity. 2008;101:75–82.View ArticlePubMedPubMed CentralGoogle Scholar
- Ahmed AM. Mosquito autogeny in Aedes caspius (Diptera: Culicidae): alterations of larval nourishments reservation upon bacterial infection. Insect Sci. 2013;20:472–84.View ArticlePubMedGoogle Scholar
- Gaio AD, Gusmão DS, Santos AV, Berbert-Molina MA, Pimenta PFP, Lemos FJA. Contribution of midgut bacteria to blood digestion and egg production in Aedes aegypti (Diptera: Culicidae) (L.). Parasit Vectors. 2011;4:105.View ArticlePubMed CentralGoogle Scholar
- Minard G, Mavingui P, Valiente MC. Diversity and function of bacterial microbiota in the mosquito holobiont. Parasit Vectors. 2013;6:146.View ArticlePubMedPubMed CentralGoogle Scholar
- Backhed F, Ding H, Wang T, Hooper LV, Young Koh G, Nagy A, et al. The gut microbiota as an environmental factor that regulates fat storage. Proc Natl Acad Sci U S A. 2004;101:15718–23.View ArticlePubMedPubMed CentralGoogle Scholar
- Ridley EV, Wong CAN, Westmiller S, Douglas AE. Impact of the resident microbiota on the nutritional phenotype of Drosophila melanogaster. PLoS One. 2012;7(5):e36765.View ArticlePubMedPubMed CentralGoogle Scholar
- Semova I, Carten JD, Stombaugh J, Mackey LC, Knight R, Farber SA, Rawls JF. Microbiota regulate intestinal absorption and metabolism of fatty acids in the zebrafish. Cell Host Microbe. 2012;12:277–88.View ArticlePubMedGoogle Scholar
- Newell PD, Douglas AE. Interspecies interactions determine the impact of the gut microbiota on nutrient allocation in Drosophila melanogaster. Appl Environ Microbiol. 2014;80:788–96.View ArticlePubMedPubMed CentralGoogle Scholar
- Sison-Mangus MP, Mushegian AA, Ebert D. Water fleas require microbiota for survival, growth and reproduction. ISME J. 2015;9:59–67.View ArticlePubMedGoogle Scholar
- Shin SC, Kim SH, You H, Kim B, Kim AC, Lee KA, et al. Drosophila microbiome modulates host developmental and metabolic homeostasis via insulin signaling. Science. 2011;334:670–4.View ArticlePubMedGoogle Scholar
- Storelli G, Defaye A, Erkosar B, Hols P, Royet J, Leulier F. Lactobacillus plantarum promotes Drosophila systemic growth by modulating hormonal signals through TOR-dependent nutrient sensing. Cell Metab. 2011;14:403–14.View ArticlePubMedGoogle Scholar
- Ziegler R, van Antwerpen R. Lipid uptake by insect oocytes. Insect Biochem Mol Biol. 2006;36:264–72.View ArticlePubMedGoogle Scholar