Plasmodium reichenowi EBA-140 merozoite ligand binds to glycophorin D on chimpanzee red blood cells, shedding new light on origins of Plasmodium falciparum
© The Author(s). 2017
Received: 23 May 2017
Accepted: 30 October 2017
Published: 7 November 2017
All symptoms of malaria are caused by the intraerythrocytic proliferation of Plasmodium merozoites. Merozoites invade erythrocytes using multiple binding ligands that recognise specific surface receptors. It has been suggested that adaptation of Plasmodium parasites to infect specific hosts is driven by changes in genes encoding Plasmodium erythrocyte-binding ligands (EBL) and reticulocyte-binding ligands (RBL). Homologs of both EBL and RBL, including the EBA-140 merozoite ligand, have been identified in P. falciparum and P. reichenowi, which infect humans and chimpanzees, respectively. The P. falciparum EBA-140 was shown to bind human glycophorin C, a minor erythrocyte sialoglycoprotein. Until now, the erythrocyte receptor for the P. reichenowi EBA-140 remained unknown.
The baculovirus expression vector system was used to obtain the recombinant EBA-140 Region II, and flow cytometry and immunoblotting methods were applied to characterise its specificity.
We showed that the chimpanzee glycophorin D is the receptor for the P. reichenowi EBA-140 ligand on chimpanzee red blood cells.
We propose that the development of glycophorin C specificity is spurred by the P. falciparum lineage. We speculate that the P. falciparum EBA-140 evolved to hijack GPC on human erythrocytes during divergence from its ape ancestor.
Malaria causes over a half million deaths per year, chiefly among children and pregnant women in sub-Saharan Africa, and most of the cases are caused by Plasmodium falciparum . A closely related species, Plasmodium reichenowi, infects chimpanzees [2, 3]. Until 2009, only one P. reichenowi isolate was genetically characterised . Due to the morphological similarity of these two species, it was initially suggested that P. falciparum originated from P. reichenowi, most likely by a single transfer from chimpanzees (Pan troglodytes) [5, 6] or evolved in bonobos (Pan panicus) . However, it was previously shown that while humans cannot be infected by P. reichenowi, P. falciparum can infect chimpanzees, albeit without severe symptoms as seen in humans [3, 8].
Recently, the use of molecular tools for species identification to explore the diversity of Plasmodium species, have revealed new phylogentic species in great apes [6, 7, 9, 10]. As a result, in the old subgenus Laverania, six Plasmodium species were confirmed, of which P. reichenowi, P. gaboni and P. billcollinsi only infect chimpanzees, whereas P. praefalciparum, P. adleri and P. blacklocki only infect gorillas. Indeed, it was proposed that great apes are natural hosts to diverse Plasmodium species, including P. falciparum previously considered as strictly human-specific .
Sequencing of Plasmodium DNA from a large collection of ape fecal samples revealed the closest relative, and likely origin of human P. falciparum, is a clade of parasites found in the western gorillas . This finding suggested a possible gorilla origin of human P. falciparum, in opposition to previous theories proposing chimpanzee-human transmission of P. reichenowi-related parasite. In particular, all known strains of P. falciparum circulating in humans nowadays resulted from a single cross-transmission event from gorilla to human . These data suggested that ape-to-human transmission was possible, but an alternative theory that each parasite species evolved independently along with its host cannot be ruled out [12–15].
The genomic basis of the P. falciparum adaptation to human hosts was explored by sequencing the genomes of two closely related species, P. reichenowi and P. gaboni, parasitic in chimpanzees. While it was shown that the genomes of P. falciparum and P. reichenowi are remarkably similar, striking differences were found in the genes involved in red cell invasion, which determine host specificity [9, 16, 17]. Invasion of erythrocytes by Plasmodium parasites involves multiple ligands; merozoites bind to erythrocytes using proteins that belong to two families including erythrocyte-binding ligands (EBL) and reticulocyte-binding ligands (RBL) [18, 19]. It was suggested that changes in the sequence and arrangement of genes in the EBL and RBL family may be directly associated with Plasmodium adaptation to its host.
Several proteins in the P. falciparum EBL family recognise different human red blood cell receptors and thus enable the merozoite to gain entry through alternative invasion pathways. Four functional P. falciparum EBL proteins have been identified so far: erythrocyte-binding antigen-175 (EBA-175), erythrocyte-binding antigen-181 (EBA-181), erythrocyte-binding ligand-1 (EBL-1) and erythrocyte-binding antigen-140 (EBA-140) . These proteins contain several conserved regions, such as Region II, which is involved in binding receptors on erythrocytes.
Recent results from the Malaria Genomic Epidemiology Network Project  demonstrated that resistance to malaria may be linked to the cluster of genes encoding human glycophorins, which are surface sialoglycoproteins of erythrocytes . Thus, it may be argued that changes in the region of genes encoding glycophorins A, B, and possibly E, all of which may act as receptors for merozoite EBL proteins, arose as the result of strong evolutionary pressure exerted by P. falciparum on the human genome. These data emphasized the crucial role that EBL proteins and glycophorins play when merozoites burgle erythrocytes.
Plasmodium falciparum merozoite ligands that bind glycophorins show distinct binding behaviors, which result in different invasion pathways. The well-studied P. falciparum EBA-175 recognizes glycophorin A (GPA) [23–25], while EBA-140 [26–28] was shown to bind glycophorin C (GPC) [29–33], a minor erythrocyte sialoglycoprotein [32, 34]. Both EBA-140 and EBA-175 bind to erythrocytes in a sialic acid-dependent manner , but binding of EBA-140, also, requires that GPC is N-glycosylated . EBA-175 recognises sialic acids present on clusters of O-linked glycans of glycophorin A (GPA). Homologs of merozoite EBL and RBL, including the EBA-140 protein, were identified in P. reichenowi [37, 38]. The amino acid sequences of the P. falciparum and P. reichenowi EBA-140 proteins are 81% identical, and the highest degree of similarity is seen within the binding region (Region II). However, the binding specificity of the P. reichenowi EBA-140 is still poorly understood. Both ligands require sialic acid for binding. Using the surface expression of human and chimpanzee EBA ligands on COS 7 cells, it was shown that the P. falciparum EBA-140 requires Neu5Ac, while its P. reichenowi counterpart requires Neu5Gc . Thus, the difference in the binding specificity of these proteins may have arisen in response to the change of host “sialome” during the evolution of the human-specific Plasmodium species . The human lineage lost the ability to turn Neu5Ac into Neu5Gc as a result of a mutation in the CMAH gene, which encodes the CMP-Neu5Ac hydroxylase. Thus, it has been hypothesized that the difference in sialic acid structure between humans and apes is the primary factor determining species-specific binding of malaria parasites [8, 39]. However, it was found that the EBA-175 ortholog from the chimpanzee-restricted parasites binds to human GPA with a similar affinity to that of P. falciparum, which suggests that the EBA 175-GPA interaction is probably not the sole determinant of Plasmodium host specificity . Moreover, it was proposed that the interaction of the P. falciparum Rh5 RBL ligand with basigin on erythrocytes is a major determinant of host species tropism.
There is a general agreement that P. falciparum recognises GPC on human erythrocytes [29–33]. GPC is encoded in humans by the GYPC gene, which is unique among the glycophorin genes because it contains two separate translation initiation sites [41, 42]. This leads to the synthesis of (predominantly) GPC and its truncated form, called glycophorin D (GPD), which lacks the first 21 aa residues of GPC. It was shown that GPC, but not GPD, plays a role in erythrocyte invasion mediated by P. falciparum EBA-140 [29–32]. Also, GPC (but not GPD) contains N-glycan at the Asn8 residue, the presence of which seems to be necessary for receptor recognition [35, 36, 43]. The sequences of the GYPC gene homologs in six Hominidae species (human, chimpanzee, bonobo, gorilla, orangutan and white-cheeked gibbon) reveal a C to A transversion, which results in the emergence of a new start codon present only in humans . Thus, humans are the only species that produce both GPC and GPD, with GPC being the major gene product. Therefore, it was suggested that GPD might be an ancestral receptor in nonhuman primates for P. reichenowi-like parasites, while GPC emerged as a new receptor in humans, targeted by P. falciparum .
To explain the role of GPC and GPD in P. reichenowi binding, we used the recombinant binding region (Region II) of P. reichenowi EBA-140 obtained in baculovirus expression vector system . We demonstrate that the receptor for the P. reichenowi EBA-140 is probably the homolog of human glycophorin D on chimpanzee erythrocytes.
Chimpanzee (Pan troglodytes) blood was freshly collected on EDTA during non-experimental clinical veterinary practice in the Warsaw Zoological Garden. The blood was drawn from immobilised chimpanzee female during a medical intervention. This sample was used to perform diagnostic tests, and the intact remainder (0.5 ml) was used in our experiments.
Flow cytometry analysis
The recombinant P. reichenowi EBA-140 Region II was incubated in phosphate buffered saline (PBS), pH 7.4 for 2 h at 4 °C with 3 × 105 native and trypsin- and chymotrypsin- (Sigma-Aldrich, St. Louis, MO, USA) treated chimpanzee erythrocytes. The heat-denatured Region II was used as the negative binding control. The cells were washed three times with PBS and incubated for 1 h at 4 °C with rabbit serum (diluted 1:200) raised against the whole P. falciparum EBA-140 Region II . The cells were washed three times with PBS and incubated for 45 min at 4 °C with FITC-conjugated swine anti-rabbit Ig antibody (DakoCytomation, Glostrup, Denmark) and analyzed for fluorescence intensity using flow cytometry (FACSCalibur, BD Biosciences, San Jose, USA). Mouse monoclonal antibody (MoAb) (clone 2G11 , diluted 1:500) recognizing human GPC and the chimpanzee GPD homolog was used as the binding control.
Western blotting (overlay assay)
Proteins of native and enzyme-treated chimpanzee erythrocytes or human erythrocyte membranes were fractionated by SDS-PAGE using a 10% polyacrylamide gel according to the Laemmli method  and then transferred to a nitrocellulose membrane (Schleicher & Schuel, Dassel, Germany) according to the method of Towbin et al. . The membranes were overlaid with the solution of P. reichenowi or P. falciparum recombinant Region II (100 μg/ml) in TBS overnight at room temperature. The bound Region II was detected with a mouse MoAb directed against the c-myc epitope (clone 9E10, ATCC, diluted 1:10). Erythrocyte GPD was detected on the blots with MoAb 2G11  (diluted 1:500) recognizing N-terminal epitope (amino acid residues 14–20/14–18) and MoAb 1F6  (diluted 1:50) recognizing C-terminal fragment (amino acids 110–115/89–94) on human GPC and GPD, respectively. The PageRuler Prestained Protein Ladder (Fermentas,Villnius, Lithuania) was used as a protein standard.
Binding of the P. reichenowi EBA-140 region II to chimpanzee erythrocytes
Binding of EBA-140 region II to glycophorin D
While genomes of P. falciparum and P. reichenowi are remarkably similar, large differences in the genes involved in red cell invasion (which determine host specificity), stand out against that conserved background . It was shown that these genomes are essentially co-linear in the core central regions with a small number of significant differences. The most striking of these involve genes associated with red cell invasion. Within the five-member EBL family, EBA-165 is a pseudogene in P. falciparum but not in P. reichenowi, while EBL-1 has a substantial deletion in P. reichenowi. Similarly, of six RBLs in the P. falciparum genome, only three (Rh2b, Rh4, Rh5) have orthologues in P. reichenowi. The Rh2 locus seems to be, the most different between these two species. Notable differences can also be seen between the rif and stevor multigene families where the numbers are much lower in the human parasite .
Moreover, genes encoding other erythrocytic malaria antigens: MSP2  and var2CSA (which is associated with malaria in pregnancy ), have shown recently extended polymorphism in P. falciparum that likely originated after the P. reichenowi-P. falciparum split . Thus, it was hypothesized that changes in the sequence and arrangement of genes encoding erythrocytic stage antigens, especially the EBL and RBL proteins, may be directly associated with Plasmodium human adaptation. Additional support for this hypothesis comes from the evaluation of dimorphism of P. falciparum EBA-175 alleles  and human specificity of Rh5 ligand in receptor-binding preferences . Explaining how this host-switch occurred in the evolution of P. falciparum lineage remains still one of the greatest challenges.
There is general agreement that GPC is the sialylated receptor on human erythrocytes for the P. falciparum EBA-140 [29–32]. Since EBA-140 ligand does not recognize GPA, which is also a highly glycosylated protein, it may be presumed that the GPC protein backbone is involved in the binding. Location of the binding site of EBA-140 close to N- and O-glycans at the N-terminal portion of GPC and lack thereof in its truncated form, GPD, may explain why the P. falciparum EBA-140 Region II does not bind to GPD.
It was shown before that GPC, but not GPD plays a role in human erythrocyte invasion mediated by P. falciparum EBA-140 ligand [29–32]. Also, only GPC is N-glycosylated at the Asn8 residue, and the N-glycan seems to be necessary for receptor recognition [35, 36, 43]. It is presumed that P. falciparum EBA-140 ligand does not bind to human GPD , due to the lack of N-glycan. Thus, it may be speculated that the P. falciparum EBA-140 ligand lost the ability of its ancestor to bind GPD, but developed the specificity for GPC, involving its N-glycan and O-glycans.
Our results show that the chimpanzee GPD is the receptor for the P. reichenowi EBA-140 on chimpanzee erythrocytes. Also, these results hint that development of EBA-140 GPC specificity may have helped P. falciparum to thrive in human erythrocytes when the human and chimpanzee lineages diverged from their ancestor.
We are indebted to qualified veterinarians Dr Agnieszka Czujkowska and Dr Andrzej Kruszewicz from the Warsaw Zoological Garden for collecting the chimpanzee blood sample for our research.
This project was supported by grant No N N302 281436 from the Ministry of Science and Higher Education of Poland and grant No 2012/05/N/NZ6/00667 from the National Centre of Science. Publication was supported by Wroclaw Centre of Biotechnology, programme The Leading National Research Centre (KNOW) for years 2014–2018.
Availability data and materials
All data generated or analysed during this study are included in this published article.
AZ performed the experiments and drafted the paper. RK proofed the manuscript. MCZ and EJ conceived the experiments and analysed the data. EJ helped write the manuscript. All authors read and approved the final manuscript.
Ethics approval and consent to participate
Chimpanzee erythrocytes were freshly collected on EDTA during non-experimental routine veterinary practice in the Warsaw Zoological Garden. The blood was taken from an immobilized female chimpanzee during medical intervention by Dr Agnieszka Czujkowska for the purposes of performing diagnostic tests. After the tests had been completed, the remaining blood (0.5 ml) was donated to us. The Warsaw Zoological Garden gave consent for us to use this blood for the purposes of our research.
Consent for publication
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