Fig. 2
Phylogenetic tree forĀ Opisthorchis sp. BD2013 (indicated by diamond symbol) and other opisthorchiids and representative trematodes from 4 families, theĀ Opisthorchiidae, Heterophyidae, Fasciolidae and Schistosomatidae (the latter used as the outgroup), based on combined nucleotide sequences of theĀ nuclear small ribosomal subunit (18S rDNA) and large ribosomal subunit (28S rDNA). Phylogenetic reconstruction was performed using maximum likelihood analysis with the general time-reversible model and a gamma distributed rate heterogeneity and proportion of invariant sites (GTR + Ī+ I) in the MEGA6.06 software package. Support for each node was evaluated using 1000 bootstrap resamplings [21]. The node for the superfamily (infraorder) Opisthorchioidea is indicated by an arrow. The scale-bar indicates the number of substitutions per site. Accession numbers are given at the end of each sequence name. Isolates or geographical localities and country isolated are given in the between (if available)