- Open Access
A Wolbachia triple-strain infection generates self-incompatibility in Aedes albopictus and transmission instability in Aedes aegypti
© The Author(s). 2018
- Received: 20 February 2018
- Accepted: 23 April 2018
- Published: 11 May 2018
Artificially-introduced transinfections of the intracellular bacterium Wolbachia pipientis have the potential to reduce the vectorial capacity of mosquito populations for viruses such as dengue and chikungunya. Aedes albopictus has two native strains of Wolbachia, but their replacement with the non-native wMel strain blocks transmission of both viruses. The pattern of cytoplasmic incompatiiblity generated by wMel with wild-types is bidirectional. Novel-plus-native-strain co-infection is predicted to lead to a more efficient population spread capacity; from a bi-directional to a uni-directional cytoplasmic incompatibility (CI) model.
A novel-plus-native-strain triple-infection in Ae. albopictus (wAlbAwAlbBwMel) was generated. Although triple-infected females were fully reproductively viable with uninfected males, they displayed self-incompatibility. qPCR of specific strains in dissected tissues suggested that this may be due to the displacement of one of the native strains (wAlbA) from the ovaries of triple-infected females. When the triple strain infection was transferred into Aedes aegypti it displayed an unexpectedly low level of transmission fidelity of the three strains in this species.
These results suggest that combining Wolbachia strains can lead to co-infection interactions that can affect outcomes of CI and maternal transmission.
- Aedes albopictus
- Aedes aegypti
Wolbachia pipientis is a maternally transmitted bacterial endosymbiont that is naturally carried by a broad range of terrestrial arthropods. The generation of novel Wolbachia transinfections in mosquitoes can result in reduced host permissivity for a range of pathogens [1–10]; for example, Aedes albopictus females transinfected with the Wolbachia strain wMel do not transmit dengue  or chikungunya  viruses in laboratory challenges. Wolbachia can invade host populations by increasing the relative fitness of infected females through a pattern of crossing sterility known as cytoplasmic incompatibility (CI) . CI occurs when a male infected with a Wolbachia strain mates with either an uninfected female or a female carrying a different non-compatible Wolbachia strain, and results in fertilization but inviability of the developing embryo. CI can be categorised according to whether populations show uni- or bi-directional sterility. In the simplest form of unidirectional CI, Wolbachia-carrying females are fully compatible with both Wolbachia-infected and Wolbachia-naïve males, but naïve females are compatible with naïve males only. Wolbachia-carrying females therefore do not suffer the fitness costs resulting from incompatible matings.
The fitness advantage of CI for Wolbachia-carriers increases with population infection frequency (with uninfected females experiencing higher proportions of incompatibility), although fitness benefits may still be appreciable at low frequencies. In the case of bidirectional CI, two interbreeding populations carry reciprocally incompatible Wolbachia infections; males and females of each infection type only producing viable progeny with carriers of the same infection. Females infected with the lower frequency strain will experience a higher proportion of incompatibility and will tend towards lower relative fitness. Spread through bidirectional CI is therefore typically associated with higher invasion threshold frequencies compared to unidirectional CI.
Crosses involving hosts carrying Wolbachia superinfections have shown that the effects of separate CI modifications can be additive [12–14]. Unidirectional CI can be produced when a superinfected line is crossed with a population carrying constituent strain(s) of the superinfection. Achieving population replacement with Wolbachia necessitates the release of infected female carriers; for public acceptance, logistical and economic reasons, it is desirable to release as few Wolbachia-carrying mosquitoes as possible to achieve self-sustaining spread. As minimum threshold frequencies for successful population invasion are typically lower under a uni-directional CI model, the generation of superinfections in naturally infected species consisting of a novel Wolbachia transinfection in addition to any native strains should allow for population replacement with fewer releases. Moreover, superinfected strains may allow for higher intracellular densities to be achieved than with a single constituent strain, resulting in improved pathogen inhibition. Joubert and colleagues  reported the generation of a superinfection in Aedes aegypti in which the wMel and wAlbB Wolbachia strains were combined, and found more robust dengue virus inhibition in the superinfected strain compared to infections with the single constituent strains. The wMelwAlbB line also showed a strong uni-directional CI crossing pattern with both wMel and wAlbB-only carriers, suggesting that the wMelwAlbB superinfection would be able to drive through single-infected populations, and could potentially rescue a control programme in the event that the dengue-blocking effectiveness of a particular strain diminished over time. Superinfections also allow the potential mixing of desirable strain phenotypes; a wAuwAlbB double-infection in Ae. aegypti combined the robust pathogen blocking capacity of Wolbachia strain wAu with the strong CI induction of wAlbB .
We attempted to create a native plus novel strain triple superinfection in Aedes albopictus, a highly invasive dengue, chikungunya and Zika virus vector with a largely suburban/semi-rural distribution. Aedes albopictus is naturally superinfected at high prevalence with two Wolbachia strains termed wAlbA and wAlbB, with wAlbA existing at approximately 10% the intracellular density of wAlbB . Aedes albopictus was previously cured of its native Wolbachia infections  and transinfected with wMel from Drosophila melanogaster; this line showed strong reductions in the transmission of dengue  and chikungunya  viruses. The wMel infection also showed bidirectional CI with wild-type (wAlbAwAlbB) infected mosquitoes . Here we report the generation of a wMelwAlbAwAlbB triple superinfection in Ae. albopictus and assess its stability, crossing types and intracellular densities. We also describe the transfer of the triple superinfection to Ae. aegypti by embryonic microinjection, and assess its stability in this species.
Superinfection generation and maternal transmission in Ae. albopictus
A triple strain superinfection comprising the native strains wAlbA, wAlbB and a novel strain wMel was generated in Ae. albopictus (hereon, wAlbAwAlbBwMel) through the transfer of cytoplasm from a wMel infected Ae. albopictus line into wild-type (wAlbAwAlbB-carrying) embryos. Aedes albopictus wAlbAwAlbBwMel females were out-crossed to Wolbachia-negative males for three generations. At G4 maternal inheritance rates were assessed by crossing females of the triple-infected line with Wolbachia-negative males, and the resulting progeny were assessed for Wolbachia infection status by strain-specific PCR. The wAlbAwAlbBwMel line showed high rates of maternal transmission of all three Wolbachia strains to progeny: of 100 individuals tested, 97 contained all three strains while 3 carried wMel only.
CI crossing types in Ae. albopictus
Percentage hatch rates (± SD) of eggs resulting from crosses between Ae. albopictus lines
92.1 ± 4.2
88.2 ± 3.2
7.0 ± 6.7
5.8 ± 4.6
67.5 ± 13.8
65.9 ± 6.9
73.7 ± 5.6
77.9 ± 12.1
88.5 ± 4.0
Wolbachia densities in Ae. albopictus wAlbAwAlbBwMel
As the fitness of host and Wolbachia depends on maximising mosquito reproductive output, it is expected that co-evolutionary pressures will favour native Wolbachia localisation to the germline, and restrict densities in tissues not directly involved in vertical transmission (where high densities can lead to reductions in host fitness). Consistent with this, the native wAlbA and wAlbB strains were found to be localised largely in the ovaries (Fig. 2a), while the novel wMel infection showed a far more diffuse tissue distribution, reaching relatively high densities in the somatic tissues of the midgut (Fig. 2b) and salivary glands (Fig. 2c).
Triple-infection transfer and maternal transmission in Ae. aegypti
Maternal transmission fidelity of Wolbachia strains in Ae.aeg-wAlbAwAlbBwMel
The Ae. aegypti wAlbAwAlbB line was out-crossed to wild-type males and displayed complete (100%) maternal transmission of both strains over a large number of generations (screening > G10), suggesting that the presence of wMel was the cause of the observed transmission infidelity in the triple-strain superinfected line.
Aedes aegypti wMelwAlbAwAlbB crossing types
A series of individual male and female single crosses were set up involving the triple-infected and wild-type mosquitoes. After eggs from single crosses were obtained, the infection status was assessed by PCR and only the eggs from crosses involving PCR-confirmed triple infected individuals were hatched and used in assessing crossing types.
Percentage hatch rates (± SD) of eggs resulting from crosses between Ae. aegypti lines
21.3 ± 12.4
76.5 ± 9.2
87.4 ± 4.1
Crossing types of Ae. aegypti infected with either wAlbA-only, wAlbB-only, a superinfection of wAlbA and wAlbB, or uninfected (wt)
The wMel-only infection in Ae. albopictus was previously reported to produce bidirectional CI with wild-type mosquitoes , but provided strong pathogen inhibition against the dengue  and chikungunya (8) viruses with minimal effects on host fitness . We created a triple-infected line combining wMel with the native strains wAlbA and wAlbB, with the aim of creating a wMel-carrying line that produces unidirectional CI with wild populations, and is thus expected to have improved population invasion ability. While males of the triple infected wAlbAwAlbBwMel line were able to successfully induce CI when crossed with wild-type females, the wAlbAwAlbBwMel line showed self-incompatibility. The wAlbAwAlbBwMel females could successfully rescue CI from wMel-only males, but were incompatible with wild-type males, suggesting an inability to rescue CI from wAlbA and/or wAlbB. The observation of significantly reduced densities of wAlbA in wAlbAwAlbBwMel ovaries compared to wild-type suggests that wAlbA in embryos reaches too low a density to be able to rescue the CI modification produced by the wAlbA strain in the males.
The self-incompatibility of the wMelwAlbAwAlbB superinfection in Ae. albopictus means that it is unlikely to be a useful alternative for dengue control, and the wMel-only Ae. albopictus line remains the best option for dengue transmission blocking in this species. Self-incompatibility will represent a potential natural barrier to new strains of Wolbachia successfully transferring into a host species that already contains one or more Wolbachia strains, if there are competitive interactions that reduce the density of one of the strains already present. The wMel infection in the triple-infected Ae. albopictus line showed a wider tissue distribution compared to the native strains, which were strongly localised to the ovaries. This finding lends support to the hypothesis that co-evolutionary selective pressures favour the localisation of native Wolbachia infections to the germline over time.
Several native plus novel strain triple infections have previously been reported in Ae. albopictus. A triple-infection consisting of wAlbA, wAlbB and wRi showed full uni-directional CI with wild-type mosquitoes and stable maternal transmission . The difference in CI crossing patterns between the triple infections carrying either wRi or wMel may be a result of the high ovarian densities reached by wMel, leading to higher levels of native strain exclusion. This suggests that an important consideration when generating further superinfected lines will be the selection of novel strains with minimal impacts on native strain density. In addition, Ae. albopictus triple infections have been generated carrying wAlbA, wAlbB and wPip  and wAlbA, wAlbB and wMelPop . In the case of the triple infection containing wMelPop, incomplete maternal transmission was observed when mosquitoes were fed a mouse-derived blood meal, with wMelPop suffering particularly low transmission fidelity. Moreover, eggs from wMelPop-containing triple-infected females displayed very low hatch rates in crosses to both triple-infected and wild-type (wAlbAwAlbB-carrying) males. Hatch rates were considerably higher (although still relatively low) when human blood was used. The authors suggest that the low hatch rates with mouse blood may be due to egg mortality resulting from elevated levels of nutrient depletion in the triple-infected embryos. An alternative explanation, supported by the present study, is that the low hatch rates are due to CI resulting from a degree of native strain exclusion in the ovaries, with the lower densities exacerbated on mouse blood. In Drosophila simulans the introduction of a third Wolbachia strain to an already naturally double-infected colony resulted in a stable triple infection, with high rates of maternal transmission and additive CI . Naturally occurring triple infections have also been detected in the white fly, Bemisia tabaci , the adzuki bean beetle, Callosobruchus chinensis , and the parasitoid wasp, Trichogramma ostriniae .
We also transferred the triple infection into Ae. aegypti, where an unexpectedly high rate of Wolbachia strain transmission infidelity was observed. Given that the lines produced are all bidirectionally incompatible, the results presented here may indicate that an upper limit exists with certain strain combinations to the number of Wolbachia infections that can stably infect a line, particularly with novel hosts such as Ae. aegypti. This limit will likely be dependent on the densities of the infecting strains, and should be lower for multi-strain infections involving high density strains due to competitive interactions.
This study presents results assessing the stability and population invasion potential for a wAlbAwAlbBwMel triple Wolbachia infection in Ae. albopictus and Ae. aegypti. In Ae. albopictus the triple infection showed high rates of vertical transmission, but a self-incompatible CI phenotype, suggesting that the triple-infection would be unlikely to persist in wild populations. It is probable that the self-incompatible phenotype is a result of an observed displacement of the wAlbA strain in the ovaries of triple-infected females, which could result in diminished CI rescue. In Ae. aegypti the triple infection showed remarkably low maternal transmission fidelity. These results highlight the importance of inter-strain interactions in determining Wolbachia superinfection stability and therefore utility in vector-control.
Mosquito strains and rearing
The Ae. albopictus wild-type line was the Ascoli strain colonized from San Benedetto del Tronto, Italy in 2006 by G. Favia and colleagues. The uninfected line the UjuT strain generated by tetracycline treatment . All mosquito colonies were maintained at 27 °C and 70% relative humidity with a 12-hour light/dark photocycle. Larvae were fed tropical fish pellets (Tetramin, Tetra, Melle, Germany) and adults were given access to a sucrose meal ad libitum. Blood meals were provided using a Hemotek artificial blood-feeding system (Hemotek, Blackburn, UK) using defribrinated sheep blood (TCS Biosciences, Botyl Claydon, UK). Eggs were collected by providing damp filter-paper (Grade 1 filter paper, Whatman plc, GE Healthcare, Coventry, UK) as an oviposition source and were desiccated for 5–10 days prior to hatching in water containing 1 g/l bovine liver powder (MP Biomedicals, Santa Ana, California, USA).
Generation of Wolbachia-infected lines
The wAlbAwAlbBwMel line was generated by transferring cytoplasm from wMel-carrying Ae. albopictus embryos to wild-type (wAlbAwAlbB-carrying) Ae. albopictus embryos. The Ae.aeg-wAlbAwAlbBwMel line was created by transferring cytoplasm from the triple infected (wAlbAwAlbBwMel) Ae. albopictus line to wild-type (Malaysian) Ae. aegypti. Microinjections were performed using methods described previously . Female G0 survivors were back-crossed to wild-type males, blood-fed and separated individually for oviposition. G0 females were analysed for Wolbachia infection by strain-specific PCR (see Additional file 1: Table S1 for sequences) and eggs from G0 females negative for the target transinfection were discarded. Eggs of positive females were hatched and G1’s were assessed for target transinfection G0-G1 germ-line transmission.
CI and maternal inheritance
Rates of CI induction and rescue both with wild-type and Wolbachia-negative mosquitoes were assessed by crosses involving 20 males and 10 females in each of three separate cages. A blood meal was provided and females were individualised for oviposition. Eggs were collected on damp filter paper, which was subsequently desiccated for 5 days at 27 °C and 70% relative humidity. Eggs were counted and hatched in water containing 1 g/l bovine liver powder. Larvae were counted at the L2-L3 stage to provide hatch rates.
To assess rates of maternal inheritance, females from the Wolbachia transinfected lines were crossed to uninfected males in pools of 20 males and 10 females. A blood meal was provided and females were individualised for oviposition. Resulting eggs were hatched and DNA from a selection of the resulting pupae were analysed for specific Wolbachia strains by PCR.
Wolbachia strain-specific PCR and density qPCR
For PCR analysis, genomic DNA was extracted from mosquitoes using the Livak method. For primer sequences see Additional file 1: Table S1. For measurements of Wolbachia density by qPCR, genomic DNA was extracted from mosquitoes using phenol/chloroform. Unless stated otherwise, mosquitoes used in density experiments were adults 5-days post-pupal eclosion. gDNA was diluted to 100 ng/μl using a NanoDrop spectrophotometer (Thermo Scientific, Waltham, Massachusetts, USA). A BioRad CFX-96 real-time PCR detection system was used (Bio Rad, Hercules, California, USA) with 2× SYBR-Green mastermix (Biotool, Houston, Texas, USA). Total Wolbachia density was analysed by absolute quantification against a dilution curve of a vector containing single copies of the homothorax (HTH) gene and Wolbachia surface protein (wsp).
To specifically quantify the wAlbA, wAlbB, and wMel strains, the following primers were used: wAlbA - QAdir1 and QArev2; wAlbB - 183F and QBrev2; wMel - qMel-F and qMel-R. All were normalized against HTH copies. The following program was used to run the qPCRs: 95 °C for 5 min, 40× cycles of 95 °C for 15 s and 60 °C for 30 s, followed by a melt-curve analysis.
We thank C. Herd (University of Glasgow), V. Geoghegan, K. Stainton and C. Harris (Lancaster University) for discussions and technical assistance.
The study was supported by the Wellcome Trust (202888, 108508), the Medical Research Council (ZK/16-021), and the Biotechnology and Biological sciences Research Council (BB/K004506).
Availability of data and materials
All data presented in this manuscript are available from the corresponding author upon reasonable request.
TA and SS conceived and designed the study. TA generated the strains and conducted the experiments. TA and SS analysed the data and wrote the manuscript. SS supervised the study. Both authors read and approved the final version of the manuscript.
Ethics approval and consent to participate
The authors declare that they have no competing interests.
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