Table 5 The percentage of T lymphocyte sub-populations in the spleen of Acanthamoeba spp.-infected immunosuppressed mice and control mice with low immunity

CD3+ (T lymphocytes)%

8 dpi

AM ± SD

29.21 ± 3.23

33.99 ± 2.60

U = 23, P = 0.04

Median

29.78

34.63

Range

24.75–33.23

30.94–36.46

16 dpi

AM ± SD

32.77 ± 1.62

36.07 ± 7.97

U = 14, P = 0.83

Median

33.03

33.63

Range

30.08–34.27

28.83–49.13

24 dpi

AM ± SD

31.65 ± 11.57

22.96 ± 3.10

U = 22, P = 0.05

Median

28.23

22.73

Range

21.76–51.73

19.78–26.09

CD4+ %

8 dpi

AM ± SD

77.37 ± 1.17

70.74 ± 1.67

U = 25, P = 0.01

Median

77.24

70.59

Range

74.98–79.46

68.14–73.33

16 dpi

AM ± SD

74.91 ± 1.56

77.40 ± 1.43

U = 22, P = 0.06

Median

75.60

77.36

Range

72.70–76.26

75.27–78.73

24 dpi

AM ± SD

72.14 ± 6.06

76.55 ± 5.98

U = 21, P = 0.14

Median

74.18

78.70

Range

62.53–78.17

66.38–81.85

CD8+ %

8 dpi

AM ± SD

17.74 ± 1.39

21.80 ± 0.57

U = 25, P = 0.01

Median

17.14

21.58

Range

16.14–19.47

21.16–22.62

16 dpi

AM ± SD

14.31 ± 8.43

16.86 ± 2.67

U = 13, P = 0.99

Median

16.72

16.40

Range

0.15–22.62

13.85–20.15

24 dpi

AM ± SD

14.98 ± 2.60

17.89 ± 1.19

U = 21, P = 0.09

Median

14.64

17.37

Range

12.23–18.69

16.93–19.75

CD4-/CD8- %

8 dpi

AM ± SD

3.70 ± 0.87

5.69 ± 1.60

U = 22, P = 0.05

Median

3.67

6.10

Range

2.62–4.90

3.29–7.18

16 dpi

AM ± SD

9.27 ± 8.06

4.10 ± 1.22

U = 21, P = 0.09

Median

6.67

4.48

Range

4.02–23.5

2.45–5.54

24 dpi

AM ± SD

7.22 ± 3.61

5.97 ± 2.54

U = 17, P = 0.41

Median

6.20

4.93

Range

4.13–13.45

3.81–10.3

CD4+/CD8+ %

8 dpi

AM ± SD

0.43 ± 0.06

0.43 ± 0.09

U = 14, P = 0.83

Median

0.41

0.38

Range

0.36–0.52

0.38–0.61

16 dpi

AM ± SD

0.52 ± 0.29

0.57 ± 0.13

U = 12, P = 0.99

Median

0.65

0.58

Range

0.01–0.70

0.38–0.72

24 dpi

AM ± SD

0.34 ± 0.09

0.48 ± 0.15

U = 19, P = 0.21

Median

0.38

0.44

Range

0.21–0.42

0.29–0.67