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Borrelia spp. in small mammals in Romania
Parasites & Vectors volume 12, Article number: 461 (2019)
Small mammals play an important role in the life-cycle of ticks and are reservoirs for several zoonotic pathogens. The aim of this study was to provide epidemiological data regarding the presence of Borrelia spp. in tissues of small mammals from Romania.
We examined 401 individuals belonging to 11 small mammal species collected in Romania. Collections cover the largest effort to survey these reservoirs in the country. Tissue samples were analyzed by multiplex qPCR targeting the ospA gene of Borrelia burgdorferi (s.l.) and a part of the flaB gene of B. miyamotoi. Positive samples were further analysed by conventional PCR and sequenced.
The overall prevalence of infection with Borrelia spp. in small mammal tissues was 4.9%. The most commonly detected species were B. afzelii, followed by B. garinii/B. bavariensis, B. miyamotoi and B. burgdorferi (s.s.). To our knowledge, we report for the first time the detection of Borrelia spp. in Crocidura leucodon and C. suaveolens, and B. miyamotoi in the liver of Myodes glareolus.
To our knowledge, our study evaluates for the first time the occurrence of Borrelia spp. in small mammals in Romania, contributing to a better knowledge of the distribution of these bacteria. This survey upgrades previous data on the spatial distribution of the pathogens and reveals the importance of animal surveillance regarding Lyme borreliosis and relapsing fever caused by B. miyamotoi.
Small mammals (Soricomorpha and Rodentia) are important reservoirs for many zoonotic tick-transmitted pathogens . Several species of the genus Ixodes may serve as bridge vectors for Borrelia spp. that allow their circulation among different hosts, including small mammals  that are common hosts for the immature tick. Synanthropic micromammal species have been widely investigated because they act as reservoirs in the natural transmission cycles of Borrelia spp. . Moreover, they are considered epidemiological markers in evaluating the distribution of certain tick species [4,5,6,7]. Although the infection rate with Borrelia spp. in these hosts is usually low, their local abundance may confer them an important epidemiological role .
Romania has a wide range of habitats colonized by species of small mammals and ticks. The small mammal-vector associations in Romania have been investigated by Mihalca et al. , who showed that the majority of the ticks on these vertebrates were I. ricinus, the most important tick parasitizing humans  and the only known vector for Lyme borreliosis in Europe. People living in rural areas in Romania are in close contact with the habitats preferred by small mammals and their ticks. This promotes a serious risk by tick-borne infectious agents that have a major impact on public health . The aim of this study was to provide epidemiological data from a survey of the presence of Borrelia spp. in small mammals from Romania thus complementing existing surveys in ticks and other vertebrates.
All animals were caught in Romania during 2010–2011, as previously described . Each captured rodent was identified to the species level. A total of 401 small mammals of 11 species (Apodemus agrarius, A. flavicollis, A. sylvaticus, A. uralensis, Micromys minutus, Microtus agrestis, M. arvalis, M. subterraneus, Myodes glareolus, Crocidura leucodon and C. suaveolens) were collected from 7 counties of Romania. Tissue samples were collected from the animals: both the heart and liver from 393 animals (98%), only the heart from 2 animals (0.5%) and only the liver from 6 animals (1.5%).
Genetic material isolation was performed individually from tissues using a commercial DNA extraction kit (Isolate II Genomic DNA Kit; Bioline, London, UK) according to the manufacturer’s protocol. Extracted DNA was stored at − 20 °C for further analysis. For each extraction procedure, negative controls were used in order to identify possible cross-contamination.
qPCR and sequencing
Multiplex quantitative polymerase chain reaction (qPCR) was used for evaluating the presence of Borrelia spp. in tissue samples. For B. burgdorferi (s.l.) we targeted the gene encoding the outer surface protein A (ospA gene) and for B. miyamotoi a part of the flagellin B (flaB gene). The qPCR was performed as previously described [10,11,12] in a CFX96 Touch™ Real-Time PCR detection system (Bio-Rad, London, UK) in a final reaction volume of 20 μl, using IQ Multiplex Powermix (Bio-Rad).
All the qPCR positive samples were amplified by conventional PCR and sequenced (Macrogen Inc., Amsterdam, Netherlands). Conventional PCR was performed as described by Szekeres et al. , targeting the glycerophosphodiester phosphodiesterase gene (glpQ) of B. miyamotoi. For B. burgdorferi (s.l.) the 5S-23S rDNA intergenic spacer region (IGS) and the outer surface protein A (ospA) gene were targeted . Nucleotide sequences were compared with those available in GenBank using the Basic Local Alignment Search Tool. In each PCR reaction set, positive and two negative controls were included.
Statistical analysis was performed by using Epi Info™ v.7.1.5 software. The frequency, infection prevalence and its 95% confidence interval were evaluated using a Chi-square independence test. A P-value of < 0.05 was considered statistically significant.
Infection of small mammals with Borrelia spp
The overall prevalence of Borrelia spp. infection in micromammal tissues was 4.9% (95% CI: 3.2–7.5%). One out of two M. agrestis was infected (50%, 95% CI: 1.2–98.7%); infection prevalence was 14.2% in C. suaveolens (95% CI: 5.9–27.2%), 9.1% in Mi. minutus (95% CI: 0.2–41.2%), 6.2% in My. glareolus (95% CI: 0.7–20.8%), 5.6% in M. arvalis (95% CI: 1.1–15.6%), 4.7% in C. leucodon (95% CI: 0.1–23.8%), 4% in A. flavicollis (95% CI: 0.5–13.9%) and 3.4% in A. agrarius (95% CI: 0.7–9.7%). The infection prevalence was significantly different among micromammal species (χ2 = 23.6, df = 10, P < 0.01) suggesting a very different role in its intrinsic importance in the life-cycle of Borrelia spp. Three species, A. sylvaticus, A. uralensis and M. subterraneus, were not infected with Borrelia spp. (Table 1).
The diversity of Borrelia spp. in small mammal tissues
Three species of the B. burgdorferi (s.l.) complex (B. afzelii, B. garinii/B. bavariensis and B. burgdorferi (s.s.) and one species of the relapsing fever group (B. miyamotoi) were identified. The most frequently detected species was B. afzelii (70%; 95% CI: 45.7–88.1%) followed by B. garinii/B. bavariensis, B. burgdorferi (s.s.) and B. miyamotoi (10%, 95% CI: 1.2–31.7%) (Table 2).
Borrelia afzelii was detected in 8 species: A. agrarius (GenBank: KY038873), A. flavicollis (KY038874), C. leucodon (KY123664), C. suaveolens (KY123665), Mi. minutus (KY123663), M. agrestis (KY123654), M. arvalis (KY123655) and My. glareolus (KY123656). Borrelia garinii/B. bavariensis, B. burgdorferi (s.s.) and B. miyamotoi were detected in single individuals of two species each: B. garinii/B. bavariensis in A. agrarius (KY123657), B. garinii/B. bavariensis in M. arvalis (KY123658), B. burgdorferi (s.s.) in A. agrarius (KY123659), B. burgdorferi (s.s.) in M. arvalis (KY123662), B. miyamotoi in A. flavicollis (KY123660) and B. miyamotoi in My. glareolus (KY123661).
The infection rate in tissue samples
The overall infection rate was 3.2% in the heart and 2.7% in the liver, without significant difference between the tissue samples (P = 0.3) (Table 3). Four infections (20%; 95% CI: 5.7–43.7%) were detected in the heart and liver (two in A. agrarius and one each in C. sueveolens and M. agrestis), nine (45%; 95% CI: 23.1–68.5%) only in the heart (five in C. suaveolens, and one each in A. flavicollis, C. leucodon, Mi. minutus and My. glareolus) and seven (35%; 95% CI: 15.4–59.2%) only in the liver (three in My. glareolus and one each in A. agrarius, A. flavicollis, C. suaveolens and My. glareolus).
From the total of 395 heart samples, 11 (95% CI: 1.6–4.9) were infected with B. afzelii, one (95% CI: 0.1–1.4) with B. garinii/B. bavariensis and B. burgdorferi (s.s.) In the liver, B. afzelii was detected in five samples (95% CI: 0.5–2.9), while B. garinii/B. bavariensis, B. miyamotoi and B. burgdorferi (s.s.) were each detected in two samples (95% CI: 0.1–1.8) (Table 2). None of the samples were co-infected.
Regarding the distribution of species by county, B. afzelii was found in Cluj (95% CI: 3.0–9.9), Constanţa (95% CI: 0.1–6.9) and Tulcea (95% CI: 0.1–26.0), B. garinii/B. bavariensis in Constanţa (95% CI: 0.1–6.9) and Bacău (95% CI: 0.8–90.6) counties, respectively B. burgdorferi (s.s.) in Constanţa (95% CI: 0.1–6.9) and Cluj (95% CI: 0.1–2.7) counties. Borrelia miyamotoi (95% CI: 0.1–3.5) was detected in A. flavicollis and My. glareolus trapped in Cluj (Table 4). Borrelia spp. DNA sequences detected in both tissues (heart and liver) in case of one specimen were 100% identical.
Apodemus flavicollis, A. sylvaticus and My. glareolus are the most common rodent species in Europe . Their role in the circulation of Borrelia spp. is well acknowledged [9, 13, 14]. The present study shows that DNA of Borrelia spp. is prevalent in A. agrarius, A. flavicollis, C. leucodon, C. suaveolens, Mi. minutus, M. agrestis, M. arvalis and My. glareolus with a variable prevalence. Previous studies from European countries reported a slightly higher level of infection with B. burgdorferi (s.l.) in small mammal tissues. The prevalence reported in different countries is concurrent with the patchy distribution of Borrelia spp. For some species our data on prevalence was in line with other European countries, e.g. Croatia  and France  (from 7 to 7.5%) for My. glareolus.
The patterns of host association of different Borrelia spp. species are reported to differ remarkably . The life-cycle of B. afzelii is dependent on multi-trophic interactions driven by the aggregation of ticks on rodents . Borrelia afzelii has a large range of reservoirs, with small mammals commonly the most important reservoirs . Bank voles and wood mice are considered preferred hosts for larvae of I. ricinus and may promote a high infection with B. afzelii in the resulting nymphs . Moreover, B. afzelii has also been shown as the most common and widely distributed species of the Lyme group in questing [19,20,21,22] and engorged ticks [23,24,25] attached to humans, and in tissues of other vertebrates [26,27,28,29] in Romania. In this study, the majority of the small mammal species were infected with B. afzelii (70%). Similar prevalence rates were found in A. agrarius from Croatia (1.9%) and in A. flavicollis (1.5%). Studies from Hungary, Lithuania and Poland reported a slightly higher prevalence in A. agrarius and A. flavicollis [8, 30,31,32]. However, we consider that the inter-country comparison of prevalence is meaningless in this context, since the conditions of parasitism by ticks were different, as well as the environment and the period of the year for the surveys.
Small rodents have been found to be reservoirs of B. garinii/B. bavariensis and B. valaisiana. Overall, we found relatively low (10%) prevalence of B. garinii/B. bavariensis infection in small mammals. Different subtypes of B. garinii have been shown to be transmitted by birds. The distinct ecotype of B. garinii OspA serotype strains that actually corresponds to B. bavariensis utilizes rodents as reservoir hosts and has been associated with high pathogenicity in humans . Our molecular detection protocol does not differentiate between B. garinii and B. bavariensis, thus further typing and/or multi-locus sequence analyses (MLSA) should be performed to delineate between B. garinii and B. bavariensis .
Borrelia miyamotoi is a tick-borne relapsing bacterium transmitted by I. ricinus in Europe. Reports from Hungary indicate the presence of B. miyamotoi in A. flavicollis (with infection prevalence of 4.8% in ticks, 0.3% in skin, 0.5% in the spleen) . The presence of B. miyamotoi has been reported only in Asia (A. argenteus), North America (Peromyscus leucopus) and in Hungary (My. glareolus) [8, 33, 35, 36].
In the present study, shrews (C. leucodon and C. suaveolens) were found to have a higher infection rate (11.4%) in comparison to mice (3%) and voles (4.5%). Experimental studies on the reservoir role of the two insectivore species have not been performed so far. Here, we report the presence of Borrelia spp. in tissues of these species: B. afzelii was detected in the heart (C. leucodon, 1/21; C. suaveolens, 6/49) as well as in the liver (C. suaveolens, 1/48; liver and heart in a single case). As the number of the investigated shrews was relatively low, we are not able to conclude their relative contribution to the enzootic cycle of tick-borne pathogens.
The variety of natural habitats in Romania shapes a wide distribution of small mammals, the country being a natural focus for tick-borne rodent-associated zoonotic pathogens. These data, together with previous studies on the distribution of ticks and the prevalence of Borrelia spp. within them will contribute a general picture of the risk in the country.
The pathogen-vector-reservoir interaction is fundamental to understand the epidemiology and prevent tick-borne diseases. Our analyses demonstrated that voles, mice and shrews are carriers of Borrelia spp. Consequently, the presence of different Borrelia species in the tissues of small mammals is an accurate marker of their circulation. These results are of importance for public health. To our knowledge, this is the first report of Borrelia spp. DNA in C. leucodon and C. suaveolens, and the presence of B. miyamotoi DNA in the liver of My. glareolus. Future studies are necessary to evaluate the contribution of shrews to the spread and maintenance of pathogens.
Availability of data and materials
The data supporting the conclusions of this article are included within the article. Representative sequences were submitted to the GenBank database under the accession numbers KY038873–KY038874 and KY123654–KY123665.
total number of samples
number of positive samples
B. burgdorferi (s.l.)
Ostfeld RS, Levi T, Jolles AE, Martin LB, Hosseini PR, Keesing F, et al. Life history and demographic drivers of reservoir competence for three tick-borne zoonotic pathogens. PLoS ONE. 2014;9:e107387.
Foley J, Piovia-Scott J. Vector biodiversity did not associate with tick-borne pathogen prevalence in small mammal communities in northern and central California. Ticks Tick Borne Dis. 2014;5:299–304.
Gern L, Humair PF. Ecology of Borrelia burgdorferi sensu lato in Europe. In: Gray J, Kahl O, Lane R, Stanek G, editors. Lyme borreliosis: biology, epidemiology and control. Wallingford: CABI Publishing; 2002. p. 149–74.
Mihalca AD, Dumitrache MO, Sándor AD, Magdaş C, Oltean M, Györke A, et al. Tick parasites of rodents in Romania: host preferences, community structure and geographical distribution. Parasit Vectors. 2012;5:266.
Mihalca AD, Dumitrache MO, Magdaş C, Gherman CM, Domşa C, Mircean V, et al. Synopsis of the hard ticks (Acari: Ixodidae) of Romania with update on host associations and geographical distribution. Exp Appl Acarol. 2012;58:183–206.
Hanincová K, Schäfer SM, Etti S, Sewell HS, Taragelova V, Ziak D, et al. Association of Borrelia afzelii with rodents in Europe. Parasitology. 2003;126:11–20.
Briciu VT, Titilincu A, Ţăţulescu DF, Cârstina D, Lefkaditis M, Mihalca AD. First survey on hard ticks (Ixodidae) collected from humans in Romania: possible risks for tick-borne diseases. Exp Appl Acarol. 2011;54:199–204.
Szekeres S, Coipan EC, Rigó K, Majoros G, Jahfari S, Sprong H, Földvári G. Eco-epidemiology of Borrelia miyamotoi and Lyme borreliosis spirochetes in a popular hunting and recreational forest area in Hungary. Parasit Vectors. 2015;8:309.
Durden LA. Taxonomy, host associations, life cycles and vectorial importance of ticks parasitizing small mammals. In: Morand S, Krasnov BR, Poulin R, editors. Micromammals and macroparasites from evolutionary ecology to management. Tokyo: Springer; 2006. p. 91–102.
Heylen D, Tijsse E, Fonville M, Matthyse E, Sprong H. Transmission dynamics of Borrelia burgdorferi s.l. in a bird tick community. Environ Microbiol. 2013;15:663–73.
Hovius JW, De Wever B, Sohne M, Brouwer MC, Coumou J, Wagemakers A, et al. A case of meningoencephalitis by the relapsing fever spirochete Borrelia miyamotoi in Europe. Lancet. 2013;382:658.
Coipan CE, van Duijvendijk GLA, Hofmeester TR, Takumi K, Sprong H. The genetic diversity of Borrelia afzelii is not maintained by the diversity of the rodent hosts. Parasit Vectors. 2018;11:454.
Tälleklint L, Jaenson TG. Transmission of Borrelia burgdorferi s.l. from mammal reservoirs to the primary vector of Lyme borreliosis, Ixodes ricinus (Acari: Ixodidae), in Sweden. J Med Entomol. 1994;31:880–6.
Gern L, Siegenthaler M, Hu CM, Leuba-Garcia S, Humair PF, Moret J. Borrelia burgdorferi in rodents (Apodemus flavicollis and A. sylvaticus): duration and enhancement of infectivity for Ixodes ricinus ticks. Eur J Epidemiol. 1994;10:75–80.
Tadin A, Tokarz R, Markotić A, Margaletić J, Turk N, Habuš J, et al. Molecular survey of zoonotic agents in rodents and other small mammals in Croatia. Am J Trop Med Hyg. 2016;94:466–73.
Cosson JF, Michelet L, Chotte J, Le Naour E, Cote M, Devillers E, et al. Genetic characterization of the human relapsing fever spirochete Borrelia miyamotoi in vectors and animal reservoirs of Lyme disease spirochetes in France. Parasit Vectors. 2014;7:233.
van Duijvendijk G, Coipan C, Wagemakers A, Fonville M, Ersöz J, Oei A. Larvae of Ixodes ricinus transmit Borrelia afzelii and B. miyamotoi to vertebrate hosts. Parasit Vectors. 2016;9:97.
Van Duijvendijk G, Sprong H, Takken W. Multi-trophic interactions driving the transmission cycle of Borrelia afzelii between Ixodes ricinus and rodents: a review. Parasit Vectors. 2015;8:643.
Kalmár Z, Mihalca AD, Dumitrache MO, Gherman CM, Magdaş C, Mircean V, et al. Geographical distribution and prevalence of Borrelia burgdorferi genospecies in questing Ixodes ricinus from Romania: a countrywide study. Ticks Tick Borne Dis. 2013;4:403–8.
Coipan EC, Vladimirescu AF. First report of Lyme disease spirochetes in ticks from Romania (Sibiu County). Exp Appl Acarol. 2010;52:193–7.
Coipan EC, Vladimirescu AF. Ixodes ricinus ticks (Acari: Ixodidae): vectors for Lyme disease spirochetes in Romania. Exp Appl Acarol. 2011;54:293–300.
Ioniţă M, Mitrea IL, Pfister K, Hamel D, Silaghi C. Molecular evidence for bacterial and protozoan pathogens in hard ticks from Romania. Vet Parasitol. 2013;196:71–6.
Briciu VT, Meyer F, Sebah D, Tăţulescu DF, Coroiu G, Lupşe M, et al. Real-time PCR-based identification of Borrelia burgdorferi sensu lato species in ticks collected from humans in Romania. Ticks Tick Borne Dis. 2014;5:575–81.
Briciu VT, Sebah D, Coroiu G, Lupşe M, Cârstina D, Ţăţulescu DF, et al. Immunohistochemistry and real-time PCR as diagnostic tools for detection of Borrelia burgdorferi sensu lato in ticks collected from humans. Exp Appl Acarol. 2016;69:49–60.
Andersson M, Zaghdoudi-Allan N, Tamba P, Stefanache M, Chitimia L. Co-infection with ‛Candidatus Neoehrlichia mikurensisʼ and Borrelia afzelii in an Ixodes ricinus tick that has bitten a human in Romania. Ticks Tick Borne Dis. 2014;5:706–8.
Gherman C, Sándor AD, Kalmár Z, Marinov M, Mihalca AD. First report of Borrelia burgdorferi sensu lato in two threatened carnivores: the marbled polecat, Vormela peregusna and European mink, Mustela lutreola (Mammalia: Mustelidae). BMC Vet Res. 2012;8:137.
Paștiu AI, Matei IA, Mihalca AD, D’Amico G, Dumitrache MO, Kalmár Z, et al. Zoonotic pathogens associated with Hyalomma aegyptium in endangered tortoises: evidence for host-switching behaviour in ticks? Parasit Vectors. 2012;5:301.
Dumitrache MO, Paştiu AI, Kalmár Z, Mircean V, Sándor AD, Gherman CM, et al. Northern white-breasted hedgehogs Erinaceus roumanicus as hosts for ticks infected with Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum in Romania. Ticks Tick Borne Dis. 2013;4:214–7.
Dumitrache MO, Matei IA, Ionică MA, Kalmár Z, D’Amico G, Sikó-Barabási S, et al. Molecular detection of Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato genospecies in red foxes (Vulpes vulpes) from Romania. Parasit Vectors. 2015;8:514.
Paulauskas A, Ambrasiene D, Radzijevskaja J, Rosef O, Turcinaviciene J. Diversity in prevalence and genospecies of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks and rodents in Lithuania and Norway. Int J Med Microbiol. 2008;298:180–7.
Gryczyńska A, Gortat T, Kowalec M. Urban rodent reservoirs of Borrelia spp. in Warsaw, Poland. Epidemiol Infect. 2018;146:589–93.
Michalik J, Skotarczak B, Skoracki M, Wodecka B, Sikora B, Hofman T, et al. Borrelia burgdorferi sensu stricto in yellow-necked mice and feeding Ixodes ricinus ticks in a forest habitat of west central Poland. J Med Entomol. 2005;42:850–6.
Margos G, Wilske B, Sing A, Hizo-Teufel C, Cao WC, Chu C, et al. Borrelia bavariensis sp. nov. is widely distributed in Europe and Asia. Int J Syst Evol Microbiol. 2013;63:4284–8.
Margos G, Vollmer SA, Cornet M, Garnier M, Fingerle V, Wilske B, et al. A new Borrelia species defined by multilocus sequence analysis of housekeeping genes. Appl Environ Microbiol. 2009;75:5410–6.
Fukunaga M, Koreki Y. The flagellin gene of Borrelia miyamotoi sp. nov. and its phylogenetic relationship among Borrelia species. FEMS Microbiol Lett. 1995;134:255–8.
Bunikis J, Barbour AG. Third Borrelia species in white-footed mice. Emerg Infect Dis. 2005;11:1150–1.
This article was published in the framework of the European Social Fund, Human Resources Development Operational Programme 2007–2013 (project POSDRU/159/1.5/S/136893).
This work was supported by a grant of Ministry of Research and Innovation, CNCS - UEFISCDI, project number PN-III-P1-1.1-PD-2016-0974, within PNCDI III.
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Veterinary conditions regarding protection of animals used in this research are compliant according to national rules and regulations of the national (Law no. 206/2004 on good conduct in scientific research, technological development and innovation) and international (DIRECTIVE 2010/63/EU of the European Parliament on the protection of animals used for scientific purposes) legislation. The Research Bioethics Commission of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca (USAMV CN) committee reviewed and approved the document. The Research Bioethics Commission of USAMV CN approved this study.
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Kalmár, Z., Sándor, A.D., Matei, I.A. et al. Borrelia spp. in small mammals in Romania. Parasites Vectors 12, 461 (2019). https://doi.org/10.1186/s13071-019-3713-3
- Borrelia burgdorferi (s.l.)
- Borrelia miyamotoi
- Ixodes ricinus
- Small mammals