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Giardia duodenalis infection in the context of a community-based deworming and water, sanitation and hygiene trial in Timor-Leste



Giardiasis is a common diarrhoeal disease caused by the protozoan Giardia duodenalis. It is prevalent in low-income countries in the context of inadequate access to water, sanitation and hygiene (WASH), and is frequently co-endemic with neglected tropical diseases such as soil-transmitted helminth (STH) infections. Large-scale periodic deworming programmes are often implemented in these settings; however, there is limited evidence for the impact of regular anthelminthic treatment on G. duodenalis infection. Additionally, few studies have examined the impact of WASH interventions on G. duodenalis.


The WASH for WORMS cluster randomised controlled trial was conducted in remote communities in Manufahi municipality, Timor-Leste, between 2012 and 2016. All study communities received four rounds of deworming with albendazole at six-monthly intervals. Half were randomised to additionally receive a community-level WASH intervention following study baseline. We measured G. duodenalis infection in study participants every six months for two years, immediately prior to deworming, as a pre-specified secondary outcome of the trial. WASH access and behaviours were measured using questionnaires.


There was no significant change in G. duodenalis prevalence in either study arm between baseline and the final study follow-up. We found no additional benefit of the community-level WASH intervention on G. duodenalis infection (relative risk: 1.05, 95% CI: 0.72–1.54). Risk factors for G. duodenalis infection included living in a household with a child under five years of age (adjusted odds ratio, aOR: 1.35, 95% CI: 1.04–1.75), living in a household with more than six people (aOR: 1.32, 95% CI: 1.02–1.72), and sampling during the rainy season (aOR: 1.23, 95% CI: 1.04–1.45). Individuals infected with the hookworm Necator americanus were less likely to have G. duodenalis infection (aOR: 0.71, 95% CI: 0.57–0.88).


Prevalence of G. duodenalis was not affected by a community WASH intervention or by two years of regular deworming with albendazole. Direct household contacts appear to play a dominant role in driving transmission. We found evidence of antagonistic effects between G. duodenalis and hookworm infection, which warrants further investigation in the context of global deworming efforts.

Trial registration Australian New Zealand Clinical Trials Registry, ACTRN12614000680662. Registered 27 June 2014, retrospectively registered.


Giardiasis is a parasitic enteric disease caused by the protozoan Giardia duodenalis. It is one of the most frequent enteric protozoan infections worldwide, with a global disease burden of approximately 280 million cases annually. Giardia duodenalis is most prevalent in low-resource settings [1], where prevalence between 20 and 40% is common [2,3,4,5,6,7,8], with prevalences approaching 70% also reported [9,10,11]. The health impact of giardiasis ranges from asymptomatic carriage, to acute self-limiting diarrhoeal disease, to chronic gastrointestinal illness accompanied by malabsorption [1]. Chronic giardiasis in childhood has been associated with poor growth, malnutrition, and decreased cognitive function [10, 12,13,14,15]. Giardiasis is transmitted by the faecal-oral route, directly from person to person, or indirectly through contaminated water or food [1]. Zoonotic transmission is also possible, with dogs and cats posing greatest risk of transmitting Giardia to humans [16,17,18,19].

Giardia duodenalis is frequently co-endemic with other parasitic diseases that are common in low-resource settings, of which soil-transmitted helminth (STH) infection is one of the most prevalent [20, 21]. STHs are parasitic intestinal helminths, including roundworm (Ascaris lumbricoides), hookworms (Necator americanus, Ancylostoma duodenale and Ancylostoma ceylanicum), whipworm (Trichuris trichiura), and threadworm (Strongyloides stercoralis). While G. duodenalis control relies on treatment with antimicrobial agents following diagnosis, control of STH infections in endemic settings occurs mainly through large-scale mass deworming programmes, as recommended by the World Health Organization (WHO) [22]. In the context of a global commitment to controlling STH infections and other neglected tropical diseases, more than 700 million children were treated with the anthelminthic agents albendazole or mebendazole in deworming campaigns in 2017 [23].

There is limited literature regarding the impact of regular deworming on G. duodenalis infection. Albendazole is efficacious in treating giardiasis when repeated doses are given over several days [24]; however, reported efficacy of single dose albendazole, as used in deworming programmes, is variable [25,26,27]. Several cross-sectional studies in areas receiving regular deworming have noted ongoing high prevalence of G. duodenalis [3, 8], and a small randomised controlled trial (RCT) in Bangladesh described an increase in G. duodenalis infection in the context of regular deworming with mebendazole [28, 29]. Some studies have shown that STH infections are associated with a decreased risk of G. duodenalis infection [30], while others have conversely noted an increased risk of protozoan infections among those infected with STHs [9, 31].

Given transmission of giardiasis by the faecal-oral route and the role of environmental contamination, interventions aimed at improving water, sanitation and hygiene (WASH) may play a role in reducing G. duodenalis transmission. A systematic review and meta-analysis of mainly observational studies found that latrine access, latrine use, and treating household drinking water were all associated with lower odds of G. duodenalis infection [32]. The effect of WASH interventions on G. duodenalis infection has been examined in small number of intervention studies, with mixed findings. In a longitudinal study in Ethiopia, a significant reduction in G. duodenalis prevalence was observed following a school-based WASH intervention [33], while in several small RCTs, household water treatment interventions showed no effect on Giardia infections [34,35,36]. An RCT conducted in the context of the Indian Total Sanitation Campaign reported a slightly lower Giardia prevalence among children who lived in villages where a community-based sanitation intervention was implemented [37]. In the recent WASH Benefits RCTs, conducted in Kenya and Bangladesh, the impact of WASH and nutrition interventions on child growth, diarrhoea, and enteric infections was studied. In Bangladesh, a significantly lower prevalence of G. duodenalis was found following sanitation, handwashing, and combined WASH interventions [5]. However, in Kenya, no interventions reduced G. duodenalis prevalence [6]. In the Sanitation Hygiene Infant Nutrition Efficacy (SHINE) trial, conducted in rural Zimbabwe, a household-level WASH intervention had no impact on the prevalence of giardiasis among infants in the first 12 months of life, though there was evidence of reduced prevalence following a combined WASH and nutrition intervention [38].

The WASH for WORMS cluster RCT was conducted in Timor-Leste to investigate the impact of a community WASH and deworming intervention, compared to deworming alone, on intestinal parasite infections. The impact of the study intervention on the primary outcome (STH infections) has been published separately [39]. Prevalence of the protozoan infections G. duodenalis, Entamoeba histolytica and Cryptosporidum spp. were pre-specified secondary outcomes of the trial [40]. Baseline data indicated that G. duodenalis infection was relatively common, with an overall prevalence of 13%, while E. histolytica and Cryptosporidium spp. were present in less than 0.1% of the study population [41].

In this paper, we aimed to determine the impact of a community-based WASH intervention on G. duodenalis infection; examine the prevalence of G. duodenalis over time in the context of regular deworming; and identify risk factors for G. duodenalis infection.


Study design

WASH for WORMS was a two-arm cluster RCT conducted between 2012 and 2016 in the Manufahi municipality of Timor-Leste [40]. Communities in the control arm received regular community-wide deworming, while communities in the intervention arm additionally received an integrated community-based WASH intervention. Informed by initial sample size requirements based on the primary outcomes (hookworm and Ascaris spp. infections), 24 communities were enrolled in the study and randomly allocated to a study arm. Five communities (two intervention and three control) were deemed ineligible prior to trial commencement and were replaced sequentially, rather than randomly, from a pre-generated list of replacement communities. It was not feasible to implement randomisation for the additional communities due to factors related to implementation of the WASH intervention by the partner organisation, including timing and community expectations. One community in the intervention arm was subsequently lost to follow-up after study baseline. Therefore, a total of 23 communities completed the study, and 18 communities (nine intervention and nine control) followed the randomisation protocol [40]. A flow diagram of the study is shown in Fig. 1.

Fig. 1
figure 1

Flow diagram of the WASH for WORMS trial

Study intervention

The deworming intervention, delivered in both study arms, involved distributing a single 400 mg dose of albendazole to all eligible community members (those older than one year of age, excluding pregnant women in the first trimester). Children aged 12–23 months were given half a dose. Four deworming rounds, conducted at six-monthly intervals, were delivered, with a fifth round given following completion of data collection. Albendazole was delivered by members of the research team and taken under direct observation.

The WASH intervention, implemented following study baseline and prior to the first deworming round, consisted of providing access to a protected community water supply, promoting improved sanitation through encouraging household latrine construction [42], and conducting hygiene education, focused on handwashing before eating and after defecating. The WASH intervention was implemented by WaterAid Australia, an international non-governmental organisation (NGO) in conjunction with local partner NGOs. Further details regarding the WASH and deworming interventions are available in the published study protocol [40].

Data collection

Sociodemographic data (age, sex, education, employment, household income and assets), clinical information (current and recent diarrhoea), and WASH information (handwashing behaviours, shoe-wearing, defecation practices, presence and type of household latrine, household garbage disposal, and household water supply) were collected through individual and household-level questionnaires at baseline and four follow-up time points, at six-monthly intervals. Stool samples were collected from all willing community members over one year of age, at baseline and each of the four follow-up time points, immediately prior to deworming. Samples were preserved in 5% potassium dichromate and sent to the QIMR Berghofer Medical Research Institute (Brisbane, Australia) for analysis. DNA extractions were performed using the PowerSoil DNA Isolation Kit (Mo Bio, Carlsbad, CA, USA) and subjected to two multiplex real-time PCR reactions: one for detection of G. duodenalis, Cryptosporidium spp., Entamoeba histolytica and Strongyloides spp., and the other for detection of Ascaris spp., N. americanus, Ancylostoma spp. and Trichuris spp. An Equine Herpes Virus target was spiked into each sample as an extraction and internal qPCR control [43].

Statistical analysis

Prevalence of G. duodenalis infection along with 95% confidence intervals (CI) was calculated for each study arm at each time point using logistic regression models that accounted for community-level clustering using robust standard errors. The impact of the community-level WASH intervention on G. duodenalis infection was evaluated in the 18 communities that followed the randomisation protocol, across all four follow-up time points. We constructed a generalised linear mixed model, with community, household, and individual entered as random effects, to account for repeated observations on the same individuals over time, nested within households and communities. We used Poisson regression to model relative risk (RR), incorporating an interaction term between study arm and follow-up time point, and adjusting for age group and sex. We calculated RR (and 95% CI) of infection for the study intervention, compared to the control arm, at each follow-up time point, by calculating a post-estimation linear combination of coefficients and standard errors, using Wald-type methods [39].

To explore individual and household-level risk factors for G. duodenalis infection, we conducted an observational analysis of all individuals in the study cohort (i.e. in the 23 communities that completed the study), across all five study time points (baseline and four follow-ups). We again constructed generalised linear mixed models to account for clustering at the community, household, and individual level. We used Bernoulli logistic regression to model odds ratio (OR) for each predictor variable. Predictor variables included sociodemographic factors, WASH access and behaviours, household animal ownership, STH infections and season. Socioeconomic status was determined using principal component analysis as described previously [41, 44]. A full list of variables examined is provided in Additional file 1: Text S1. Univariable analyses were conducted for each potential risk factor, with variables retained if P < 0.2. Multivariable models were then constructed using a two-stage approach. “Within-domain” multivariable models were built for groups of related variables: demographic, individual socioeconomic, individual hygiene, individual sanitation, school sanitation, household socioeconomic, household hygiene, household water, household sanitation, environmental, and infection-related (see Additional file 1: Text S1). These models were built using variables retained from the univariable analysis, adjusted for age, sex, and study time point. A full model was then constructed with variables significant at P < 0.1 in the “within-domain” models, adjusted for age, sex and study time point. The final multivariable model was produced using backward stepwise regression such that age, sex, study time point, and covariates significant at P < 0.05 remained.

Finally, we examined the impact of previous G. duodenalis infections on risk of infection in the entire study cohort. For this model, the outcome variable was G. duodenalis infection at the final study time point, and the predictor variable was the number of infections at previous study time points, adjusted for age and sex, and clustering at the community and household levels. All statistical analyses were conducted using Stata version 15 (College Station, TX, USA).


At study baseline, among the 23 communities that completed the trial, 2448 participants provided informed consent to participate in the study. Of these, 1971 (80.5%) provided a stool sample that was analysed for G. duodenalis infection. Baseline characteristics of this study population are shown in Additional file 2: Table S1. Participation rates across the course of the study, including those for the subset of 18 communities that followed the randomisation protocol, are summarised in Additional file 2: Table S2. Treatment coverage of eligible community members with albendazole was high, above 90% at each study time point [39].

Giardia duodenalis infections over time

Prevalence of G. duodenalis infection across the study period in each arm, overall and disaggregated by age, is shown in Table 1. Intensity of infection, measured as qPCR cycle threshold (Cq) values, across the study period is depicted in Additional file 2: Table S3. At baseline, G. duodenalis prevalence in the intervention arm was 14.5% (95% CI: 10.1–17.6%) and in the control arm was 12.3% (95% CI: 9.6–16.3%). At the end of the study, G. duodenalis prevalence in the intervention and control arms was 17.4% (95% CI: 12.9–21.6%) and 14.0% (95% CI: 10.3–18.0%), respectively. As depicted in Table 1, in both study arms, G. duodenalis prevalence was highest among children aged 1–5 years of age, ranging between 23.7% (95% CI: 14.4–30.1%) and 34.3% (95% CI: 25.2–43.4%) in the intervention arm, and between 14.4% (95% CI: 5.5–23.1%) and 38.1% (95% CI: 27.7–48.5%) in the control arm. There was no significant difference in risk of G. duodenalis infection at the end of the study compared to baseline, in either the intervention arm (RR: 1.17, 95% CI: 0.87–1.86, P = 0.222) or the control arm (RR: 1.16, 95% CI: 0.90–1.48, P = 0.252).

Table 1 Prevalence of G. duodenalis in the study population over time

Across all age groups and study time points, the prevalence of self-reported diarrhoea (at the time of survey or within the previous 2 weeks) among those infected with G. duodenalis was 11.5% (95% CI: 7.4–17.5%). This was not significantly different between the intervention arm (14.1%, 95% CI: 7.7–24.5%) and the control arm (8.6%, 95% CI: 5.4–13.7%, P = 0.206), and was also not significantly different to the prevalence of diarrhoea reported among those not infected with G. duodenalis (11.5%, 95% CI: 9.7–13.7%, P = 0.768). Among those infected with G. duodenalis, the highest diarrhoea prevalence was observed in children aged 1–5 years in both the intervention arm (15.9%, 95% CI: 8.5–28.0%) and the control arm (11.9%, 95% CI: 6.5–20.6%).

Impact of WASH intervention

The results of the generalised linear mixed model examining the impact of the study intervention are shown in Table 2. These results demonstrate no significant difference in risk of G. duodenalis infection between the intervention or control arm at any time point. At the completion of the trial, the RR of G. duodenalis infection in the intervention arm, compared to the control arm, was 1.05 (95% CI: 0.72–1.54, P = 0.787).

Table 2 Impact of the study intervention on G. duodenalis infection

Risk factors for G. duodenalis infection

Univariable analyses found various WASH, demographic and socioeconomic variables significantly associated with G. duodenalis infection at P < 0.2; full results of univariable analyses are provided in Additional file 2: Table S4. In the final adjusted multivariable model (Table 3), the odds of G. duodenalis infection decreased significantly with increasing age. People who lived in a household with a child under five years of age had significantly increased odds of infection compared to those who did not (adjusted odds ratio, aOR: 1.35, 95% CI: 1.04–1.75, P = 0.023). Similarly, those living in a household of more than six people had higher odds of infection compared to those living in households of six or fewer people (aOR: 1.32, 95% CI: 1.02–1.72, P = 0.035). Odds of infection were higher in the wet season compared to the dry season (aOR: 1.23, 95% CI: 1.04–1.45, P = 0.015). Infection with Necator americanus was associated with lower odds of G. duodenalis infection (aOR: 0.71, 95% CI: 0.57–0.88, = 0.002). No WASH variables remained as significant predictors of infection in the final model.

Table 3 Results of final generalised linear mixed model of risk factors for G. duodenalis infection

Repeated infections in individuals over time

Table 4 shows the impact of G. duodenalis infections at previous study time points on the risk of infection with G. duodenalis at the final follow-up. The risk of G. duodenalis infection at the end of the trial was significantly greater among those with a history of infection diagnosed at previous study time points, with relative risk of infection increasing with the number of time points.

Table 4 Impact of previous infections on G. duodenalis infection at the end of the trial


To our knowledge, this is the first study to examine G. duodenalis infection in the context of a community-based WASH and deworming RCT. In our study setting in rural Timor-Leste, we observed a moderate overall prevalence of G. duodenalis in both study arms, ranging between 10.2 and 19.6% over the study period. Prevalence was highest (up to 38.1%) among children aged 15 years. This is consistent with the known epidemiology of Giardia infection that tends to predominate among young children [1, 45, 46]. Most infections appeared to be subclinical; only 11.5% of individuals infected with G. duodenalis reported diarrhoea, which was similar to the background diarrhoea prevalence among the study population. Over four rounds of community-wide deworming with albendazole, we observed no significant change in G. duodenalis prevalence in either study arm. This supports previous findings that a single 400 mg dose of albendazole is not efficacious against G. duodenalis [25].

We found no impact of the community-based WASH intervention in terms of reducing G. duodenalis prevalence, compared to the control arm that did not receive a WASH intervention. This is consistent with the findings of the primary outcome of the WASH for WORMS trial, where no additional impact of the WASH intervention on STH infections was identified [39]. Our findings are also consistent with several previous intervention studies, including the recent SHINE trial and WASH Benefits Kenya, that identified no impact of household-level WASH interventions on Giardia infection [6, 38]. However, other studies, including WASH Benefits Bangladesh, have detected lower risk of G. duodenalis infection among participants who received WASH interventions [5, 37]. A possible explanation for our findings is suboptimal coverage of the WASH intervention. As previously reported, although initial household latrine coverage was high following the WASH intervention, this decreased over time, and at the end of the trial, 40% participants in the intervention arm were practising open defecation [39]. This would facilitate environmental contamination, likely leading to ongoing transmission of G. duodenalis. Additionally, although rates of reported handwashing with soap were high among study participants at the end of the trial, this was self-reported and may therefore overestimate true handwashing behaviours [47]. Other potential sources of ongoing G. duodenalis transmission following the WASH intervention include zoonotic transmission and contaminated drinking water. Given that protected water sources were provided as part of the study intervention, contamination occurring at the main source of community drinking water is unlikely. However, it is possible that individuals obtained drinking water from other (unprotected) sources, or that water contamination occurred during or after collection. WASH interventions may require higher coverage, over a longer duration of time, to achieve any impact on transmission of intestinal protozoa in areas with significant environmental contamination.

Our risk factor analysis provides further insight into the individual-level factors associated with G. duodenalis infection. We examined a wide range of factors including WASH access and behaviours, animal ownership, STH infections, and socioeconomic variables. However, relatively few predictors remained in our final model. Age group was a major predictor of infection, and N. americanus infection was associated with reduced odds of G. duodenalis infection. This potential antagonism between hookworm and G. duodenalis has been identified previously [30], with potential explanations including competitive inhibition within the small intestine, or cross-immunity due to helminth-induced Th2 cell response [30]. However, these findings are not consistent across the literature, with other studies reporting increased risk of protozoan infections among those infected with STH [9, 31].

Other factors that significantly affected the odds of G. duodenalis infection included living in a household with a child under five years of age, and living in a household with more than six people. This demonstrates that direct household contacts likely play a dominant role in transmission. Additionally, infection was significantly more likely during the wet season, consistent with previous findings of increased transmission during rainy seasons [11, 48].

We identified that previous infection with G. duodenalis was associated with significantly increased odds of infection at the end of the study, with a dose-response observed with increasing number of previous infections. Although there is some evidence that people may develop a degree of acquired immunity to G. duodenalis [49], it has also been shown that reinfections occur commonly and rapidly after successful treatment [50]. Furthermore, it is known that giardiasis can become chronic and persist for more than six months following initial infection [14, 51]. Therefore, repeated infections within individuals in this study could represent either chronic infections, or reinfections occurring after clearing Giardia, potentially driven by persistent environmental exposure or repeated risk behaviours.

This analysis was conducted in the context of a robustly-designed RCT, using a highly-sensitive diagnostic technique; however, there are several important limitations. The community-based WASH intervention, although delivered in accordance with national sanitation policy by local NGOs in a culturally-appropriate manner, did not achieve its aims of 100% household latrine coverage and “open-defecation free” communities. This made detecting any impact of the WASH intervention less likely. As mentioned above, data relating to WASH access and behaviours were derived predominantly from self-report, which could be biased towards reporting safer WASH behaviours [47], leading to difficulty in detecting true associations between WASH behaviours and infection. Finally, despite including a wide range of potential predictors in the analysis of risk factors for G. duodenalis infection, residual confounding due to unmeasured factors remains likely.

A number of key priorities for ongoing research arise from this study. First, additional research examining the impact of WASH risk factors and WASH interventions on protozoan infections is required. Most previous studies on risk factors have focused on children [32]. Given that we identified infection across all age groups and that many households are intergenerational, more research into G. duodenalis infection across the entire community should be undertaken. Identifying improved mechanisms to measure WASH behaviours in a research context is also required, to allow accurate determination of key risk factors and inform the optimal design of interventions. Food-borne transmission is an important source of G. duodenalis infection [52, 53] and Giardia also has potential for zoonotic transmission [17,18,19]; therefore, WASH interventions may be improved by incorporating education about safe food handling practice, and hygiene around animals, tailored to local contexts.

Additional studies are required to investigate the potential antagonistic relationship between G. duodenalis and hookworm infections, and to identify ways to mitigate this potentially antagonistic effect. This is particularly important given the high burden of both STH and Giardia infections in low-resource settings, especially among children, and the large-scale global deworming efforts currently underway. It may be necessary for STH control guidelines to include recommendations for undertaking diagnosis and treatment of G. duodenalis infections during monitoring efforts, and for educating communities about the symptoms of giardiasis and the importance of seeking additional treatment given that deworming is unlikely to be effective. Strengthening the understanding of the role of both WASH and deworming in the epidemiology and control of G. duodenalis is crucial, in order to inform the design and delivery of interventions tailored to the communities that will benefit from them most.


This study demonstrates the ongoing burden of G. duodenalis across all age groups, but particularly among children, in communities receiving regular deworming for STH control. It also demonstrates some of the challenges involved in implementing and evaluating WASH interventions in low-income settings, and the complexities in generating evidence to demonstrate the impact of WASH on health outcomes. This study provides important evidence to inform the understanding of G. duodenalis epidemiology in the context of regular deworming and WASH interventions, and highlights important areas for future research, including further investigation of a potentially antagonistic relationship between hookworm and G. duodenalis infections.

Availability of data and materials

Data are available from the corresponding author on request.



adjusted odds ratio


confidence interval


cycle threshold


non-governmental organisation


odds ratio


quantitative polymerase chain reaction


randomised controlled trial


relative risk


soil-transmitted helminth


water, sanitation and hygiene


World Health Organization


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We would like to acknowledge WaterAid, particularly Alex Grumbley and Edmund Weking, and WaterAid’s local partner NGOs, for implementing the study intervention. We acknowledge the Timor-Leste Ministry of Health for providing albendazole; the WASH for WORMS field team for conducting data collection and distributing albendazole; Stacey Llewellyn for conducting the qPCR diagnostics; Suzy Campbell for contributions to questionnaire development, data cleaning, and the principal component analysis; and all of the participants in the WASH for WORMS trial.


The WASH for WORMS trial was funded by an NHMRC Partnership project in collaboration with WaterAid Australia (1013713). The funders had no role in study design, data collection and analysis, interpretation of study results, or preparation of the manuscript.

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Authors and Affiliations



JYHA and NEC conducted the statistical analysis, with input from MHH and SVN. JYHA and NEC drafted the manuscript. SVN was the scientific coordinator of the WASH for WORMS trial and was responsible for implementing the study protocol and overseeing data collection and data entry. ACAC was the principal investigator of the trial. SA was the project manager supervising data collection in the field. JSM supervised the analysis of stool samples using qPCR. RT, JSM, RMA, and DJG were study investigators who participated in designing the trial. All authors contributed to editing and revising the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Naomi E. Clarke.

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Ethics approval and consent to participate

Ethical approval was provided by the Human Research Ethics Committees at The Australian National University (2014/311), The University of Queensland (201100734) and the Timor-Leste Ministry of Health (2011/51). Written informed consent was obtained from all participants aged 18 years and over, and from parents/guardians of participants aged under 18 years.

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Not applicable.

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The authors declare that they have no competing interests.

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Supplementary information

Additional file 1: Text S1.

Full list of variables examined as risk factors for Giardia infection.

Additional file 2: Table S1.

Baseline characteristics of study participants. Table S2. Participation rates in the study over time. Table S3. Intensity of infection with G. duodenalis over time, measured using qPCR cycle threshold (Cq) values among positive samples. Table S4. Results of univariable analyses for risk factors for G. duodenalis infection (n = 2333), based on generalised linear mixed models accounting for village, household and individual-level clustering.

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Aw, J.Y.H., Clarke, N.E., McCarthy, J.S. et al. Giardia duodenalis infection in the context of a community-based deworming and water, sanitation and hygiene trial in Timor-Leste. Parasites Vectors 12, 491 (2019).

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