Fig. 3

Alignment of the domains IIS1–S6 and partial domain II–III linker fragment of the vgsc gene of C. hemipterus with Accession No. MK567761 against the reference vgsc gene of C. lectularius wild type with Accession No. FJ031996. The mutation sites are indicated in vertical boxes; red, nonsense mutation at M918 and L1014 sites, green, silent mutations with transition (at L925, K949, N950, and H995 sites), and transversion (at L936 site), and blue, novel non-synonymous mutation with transition at D953 site. Nucleotides 1–144 include exon I, 145–217 intron I, 218–380 exon II, 381–445 intron II, 446–628 exon III, 629–711 intron II, and 712–812 exon IV. NS and AS represent nucleotide sequence and deduced amino acid sequences, respectively. The location of primers are indicated with red (conventional PCR), blue (SYBR Green real-time PCR), and green (TaqMan real-time PCR) arrows