Fig. 4

Anti-osteosarcoma effect of anti-TPD52 antiserum in vivo. Schematic diagram of the experimental process. In brief, after the osteosarcoma challenge, treatment was started on day 9 with subcutaneous injections around the tumour; the trial was terminated on the 21st day (a). Osteosarcoma of nude mice from each group (b). The growth trend of osteosarcoma (c). Osteosarcoma volume in nude mice in each group (d). Osteosarcoma weight in nude mice in each group (e). Abbreviations: PBS: osteosarcoma model. NS: negative serum. TS: 100 µg anti-T. spiralis antiserum. 25 μg, 50 μg, and 100 μg: anti-TPD52 antiserum (**P < 0.01; ***P < 0.001; ****P < 0.0001; ns: non-significant)