Fig. 3
Immunofluorescence assay (IFA) on 100% acetone-fixed ISE6 cells infected with GGYV and SREV at 7, 14 and 28 days post-infection (dpi). Immunofluorescent labelling with anti-E mAb 4G2 (green), anti-dsRNA MAVRIC (red) and Hoechst nuclear stain (blue). Mock controls, uninfected cells inoculated with culture medium only. WNVKUN-infected Vero cells were used as assay controls. The log value of average virus titres (TCID50 units/ml) recovered from supernatant harvested at each time point is indicated in white text at the bottom right corner of each merged panel. *Titre below the limit of detection (< 101.3/ml). Images taken at 20 × magnification with an EPI fluorescence microscope