Fig. 1From: Evaluation of anti-malaria potency of wild and genetically modified Enterobacter cloacae expressing effector proteins in Anopheles stephensiSchematic illustration of the transmission blocking assay. Steps 1–3: Infection of mouse with Plasmodium berghei ANKA strain clone 2.34 (a shows the introduction of bacteria into the mosquito midgut via sugar bait feeding). Steps 4, 5 Sugar-fed mosquitoes are fasted for 4–6 h (4), following which they blood-feed on a P. berghei-infected mouse (5). Step 6 Dissection of the mosquito midgut, staining with 0.5% mercurochrome and counting of the infective oocysts under the light microscopeBack to article page