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Table 2 Comparison of Toxoplasma gondii results for heart fluid samples using serological methods (ELISA and IFAT) relative to magnetic capture real-time PCR to determine the best dilution for cutoff values

From: Are foxes (Vulpes spp.) good sentinel species for Toxoplasma gondii in northern Canada?

Dilutions

ID

ELISA S/P%

IFAT

 

ND

1:2

1:4

1:8

1:2

1:10

1:50

1:100

N

BJ2016-223

27.31

13.84

13.3

11.56

1 + 

N

N

N

W2015-221

13.3

2.33

1.68

1.19

N

N

N

N

Mi2015-214

21.34

2.99

1.47

1.36

N

N

N

N

LP

We2015-248

243.81

237.62

227.31

195.87

3 + 

3 + 

2 + 

1 + 

We2015-253

344.84

325.35

296.58

281.05

3 + 

3 + 

2 + 

2 + 

W2015-218

17.92

8.31

6.13

2.5

1 + 

N

N

N

HP

We2015-250

351.79

332.19

323.13

295.77

3 + 

3 + 

3 + 

3 + 

W2015-227

297.94

272.58

239.47

203.53

3 + 

3 + 

2 + 

1 + 

FN21

331.65

309.55

291.8

244.46

3 + 

3 + 

2 + 

2 + 

  1. Positive results: ELISA ≥ 70% and IFAT ≥ 1 +
  2. N negative, LP low positive on magnetic capture (Cp value > 30), HP high positive on magnetic capture (Cp value ≤ 30), ND no dilution, ELISA enzyme-linked immunosorbent assay, IFAT indirect fluorescent antibody test