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Table 2 Comparison of Toxoplasma gondii results for heart fluid samples using serological methods (ELISA and IFAT) relative to magnetic capture real-time PCR to determine the best dilution for cutoff values

From: Are foxes (Vulpes spp.) good sentinel species for Toxoplasma gondii in northern Canada?

Dilutions ID ELISA S/P% IFAT
  ND 1:2 1:4 1:8 1:2 1:10 1:50 1:100
N BJ2016-223 27.31 13.84 13.3 11.56 1 +  N N N
W2015-221 13.3 2.33 1.68 1.19 N N N N
Mi2015-214 21.34 2.99 1.47 1.36 N N N N
LP We2015-248 243.81 237.62 227.31 195.87 3 +  3 +  2 +  1 + 
We2015-253 344.84 325.35 296.58 281.05 3 +  3 +  2 +  2 + 
W2015-218 17.92 8.31 6.13 2.5 1 +  N N N
HP We2015-250 351.79 332.19 323.13 295.77 3 +  3 +  3 +  3 + 
W2015-227 297.94 272.58 239.47 203.53 3 +  3 +  2 +  1 + 
FN21 331.65 309.55 291.8 244.46 3 +  3 +  2 +  2 + 
  1. Positive results: ELISA ≥ 70% and IFAT ≥ 1 +
  2. N negative, LP low positive on magnetic capture (Cp value > 30), HP high positive on magnetic capture (Cp value ≤ 30), ND no dilution, ELISA enzyme-linked immunosorbent assay, IFAT indirect fluorescent antibody test