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Fig. 6 | Parasites & Vectors

Fig. 6

From: Ad libitum consumption of protein- or peptide-sucrose solutions stimulates egg formation by prolonging the vitellogenic phase of oogenesis in anautogenous mosquitoes

Fig. 6

Blood-feeding on day 4 post-emergence activates multiple markers of the vitellogenic phase in Ae. aegypti while ad libitum access to 10% sucrose does not. Each marker was measured on the day of blood-feeding and at 24-h intervals thereafter with age-matched sugar fed females measured at the same time: a ovary ecdysteroid production measured for 6 ovary pairs per time point; b trypsin-like serine protease activity per midgut measured for 5 midguts per time point; c VgA1 copy number measured for three abdomen pairs per time point. Relative expression of the JH responsive genes Hairy (d) and Kr-h1 (e) and 20E-responsive genes EcR (f), E74 (g) and E93 (h) normalized to the reference gene RPL8 and compared to expression levels in 4-day-old sugar-fed control females. Treatment means are shown with error bars (gray) indicating standard error. The dotted horizontal line in each graph indicates y = 1 to facilitate visual comparison between control and treatment expression levels

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