Table 1 Overview of the samples used to test the barcoding pipeline
From: A barcoding pipeline for mosquito surveillance in Nepal, a biodiverse dengue-endemic country
Sample code | Origin | Sampling year | Genera | Life stage | Storage conditions | Number of tested individuals | DNA extraction | Method to verify accuracy |
|---|---|---|---|---|---|---|---|---|
NP1 | Nepal | 2013 | Aedes, Anopheles, Mansonia | Adults | Homogenates, − 20 °C | 15 | Qiagen DNeasy | Morphology |
NP2 | Nepal | 2018 | Aedes, Anopheles, Armigeres, Mansonia | Adults | 100% ethanol, room temperature | 20 | Lucigen | Sanger |
BEL | Belgium | 2019 | Aedes | Adults | Dried, room temperature | 8 | Lucigen | Morphology |
GER | Germany | 2018/2019 | Aedes | Eggs (pools of 10) | − 20 °C | 28 pools | Qiagen DNeasy | Sanger + Morphology |