Establishment of Wolbachia infection in Aedes aegypti from Pakistan via embryonic microinjection and semi-field evaluation of general fitness of resultant mosquito population

Table 1 Competitiveness index of different ratios of F₈ WAG males measured at different ratios of RAG males in semi-field conditions

Male ratio WAG:RAG	♂ × ♀ WAG:RAG × RAG	Egg hatch^a (n eggs)	Hc-Hr	Hr-Hi	Cm/In	Competitiveness index^b
0:1	0:40 × 30 Hc	91.7% ± 5.1^a (1684)	Negative control group
1:1	20:20 × 30 Hr	56.5% ± 8.1^b (1411)	35.2 ± 11.8	56.5 ± 8.1	1	0.63 ± 0.3
3:1	30:10 × 30 Hr	18.3% ± 5.9^c (1595)	73.4 ± 11.0	18.3 ± 5.9	0.33	1.38 ± 0.6
1:0	40:0 × 30 Hi	0.0% ± 0.0^d (1338)	Positive control group

All values are given as ± standard error of the mean of triplicate measures
RAG Uninfected Aedes aegypti (control), TWAG wAlbB Wolbachia removed by tetracycline, WAG wAlbB Wolbachia-transfected colony treatment
^aDifferent lowercase letters indicate that the values are statistically different (P < 0.05) in all crosses using Tukey mean procedure test
^b\(\mathrm{Competitiveness index}=\frac{Hc-Hr}{Hr-Hi }\times \frac{Cm}{In}\), where Hc = hatch rate of eggs harvested from the cross RAG♂ × RAG♀ (compatible); Hr = hatch rate of eggs harvested from the cross WAG:RAG♂ × RAG♀; Hi = hatch rate of eggs harvested from the cross WAG♂ × RAG♀ (incompatible); Cn = number of compatible males (RAG); In = number of incompatible males (WAG)

ISSN: 1756-3305