- Research
- Open Access
- Published:
Species richness of bat flies and their associations with host bats in a subtropical East Asian region
Parasites & Vectors volume 16, Article number: 37 (2023)
Abstract
Background
Understanding the interactions between bat flies and host bats offer us fundamental insights into the coevolutionary and ecological processes in host-parasite relationships. Here, we investigated the identities, host specificity, and patterns of host association of bat flies in a subtropical region in East Asia, which is an understudied region for bat fly research.
Methods
We used both morphological characteristics and DNA barcoding to identify the bat fly species found on 11 cavernicolous bat species from five bat families inhabiting Hong Kong. We first determined the phylogenetic relationships among bat fly species. Then, we elucidated the patterns of bat-bat fly associations and calculated the host specificity of each bat fly species. Furthermore, we assembled the mitogenomes of three bat fly species from two families (Nycteribiidae and Streblidae) to contribute to the limited bat fly genetic resources available.
Results
We examined 641 individuals of bat flies and found 20 species, of which many appeared to be new to science. Species of Nycteribiidae included five Nycteribia spp., three Penicillidia spp., two Phthiridium spp., one Basilia sp., and one species from a hitherto unknown genus, whereas Streblidae included Brachytarsina amboinensis, three Raymondia spp., and four additional Brachytarsina spp. Our bat-bat fly association network shows that certain closely related bat flies within Nycteribiidae and Streblidae only parasitized host bat species that are phylogenetically more closely related. For example, congenerics of Raymondia only parasitized hosts in Rhinolophus and Hipposideros, which are in two closely related families in Rhinolophoidea, but not other distantly related co-roosting species. A wide spectrum of host specificity of these bat fly species was also revealed, with some bat fly species being strictly monoxenous, e.g. nycteribiid Nycteribia sp. A, Phthiridium sp. A, and streblid Raymondia sp. A, while streblid B. amboinensis is polyxenous.
Conclusions
The bat fly diversity and specificity uncovered in this study have shed light on the complex bat-bat fly ecology in the region, but more bat-parasite association studies are still needed in East Asian regions like China as a huge number of unknown species likely exists. We highly recommend the use of DNA barcoding to support morphological identification to reveal accurate host-ectoparasite relationships for future studies.
Graphical Abstract

Background
Interactions between parasites and their hosts are one of the most striking coevolutionary arms races [1]. Both parasites and hosts exert selective pressures over each other, leading to reciprocal adaptations over time [2, 3]. Hippoboscoidea superfamily (Diptera) members have evolved to become obligate parasites with adaptations for feeding on the blood of vertebrates. Members of Hippoboscoidea belong to one of four families, Glossinidae, Hippoboscidae, Nycteribiidae, and Streblidae [4], with the latter two being ectoparasites exclusively associated with bats (Chiroptera). Co-evolving with bats for more than 15 million years [5], bat flies are highly adapted to live on the fur and wing or tail membranes of bats; they are the most prevalent ectoparasites on bats worldwide. Some streblids develop fully functional wings after pupation and can fly to search for hosts, while others that possess rudimentary or no wings and nycteribiids, which are all wingless, can only rely on crawling behaviors to reach their hosts [4]. Approximately 230 species in 33 genera from Streblidae and 280 species in 11 genera from Nycteribiidae have been recorded to date [6]. Nycteribiid and streblid species are not equally distributed among global regions [7, 8]. Nearly 70% of streblid species are distributed within the New World tropics or subtropics, and relatively few species occur exclusively in temperate zones, whereas about 80% of nycteribiid species occur in the Old World tropics or subtropics [4]. The most recent phylogenetic study showed that Nycteribiidae is monophyletic but Streblidae is a paraphyletic group, which comprises the monophyletic New World clade and the paraphyletic Old World clade, which clusters with Nycteribiidae [9]. The diversity of bat flies and their unique relationships with host bats make them an inviting model system for studying several ecological and evolutionary questions in the past decades, including systematics and biogeography, intra- or interspecific transmission of pathogens, and host-ectoparasite interaction dynamics [10].
Dynamics of bat-bat fly interactions are complex and influenced by the interplay of numerous biotic and abiotic variables. For example, roosting site preference and roosting behaviors of bats can affect the transmissibility of their bat flies. Bat populations roosting in permanent and protective structures (e.g. caves, water tunnels, and abandoned mines) were found to experience heavier bat fly parasitism than those more temporary roosting sites, such as foliage, tree trunks, and other open spots [11,12,13]. Bats that used more caves to roost and remained in caves for longer times were more likely to be infested by ectoparasites [14]. Moreover, the morphophysiological traits of bat hosts, such as sex, body conditions, and reproductive cycle, were found to affect the host preference of bat flies or level of bat fly parasitism [15,16,17]. The life histories and morphological traits of ectoparasites are also the key determinants in their dispersal capability [18, 19]. Vegetation type and seasonality could also promote changes in the bat-bat fly interaction network in a region [20]. Recent studies demonstrated that bat flies generally exhibited high host specificity and often formed distinct species assemblages on their host bat species [21]. Individual bat species typically support one to five species of bat flies [22] with phylogenetically distant bat fly species co-occurring on a similar host occupying different areas of the host's body to reduce competition for space and resources [23].
Although the distribution, species richness, and host-ectoparasite associations of bat flies in the Americas, Africa, and Southeast Asia have been relatively well documented over the years, knowledge on bat flies in East Asia, especially China, is largely lacking [24, 25]. Considering the importance of this geographic region in multiple outbreaks of severe zoonotic diseases linked to bats [26, 27], understanding the diversity of bat flies and their association to bats in this region appears especially urgent from ecological and public health perspectives. Bats are well known to be important natural reservoirs of zoonotic pathogens [28], and bat flies were proposed to function as vectors for certain bat-associated pathogens, in which some also possess high zoonotic potential [29, 30].
One difficulty of bat fly research for understudied regions is the identification of specimens based on a few taxonomic publications developed more than half a century ago [31,32,33,34] from a limited and incomplete pool of species, a problem more widely known as the Linnean shortfall [35, 36]. Occurrences of cryptic species and phenotypic plasticity represent additional difficulties to morphologically distinguish bat fly species [33, 37]. DNA barcoding was thus recommended as a potential approach to reliably separate bat fly species [13, 37]. In this study, we sought to determine the species richness, phylogenetic relationships, and host association patterns of bat flies found on 11 cavernicolous bat species occurring in China, using both morphological and DNA barcoding approaches. Hong Kong, located in the subtropical region of East Asia, is home to 25 bat species recorded to date [38]; however, the bat flies parasitizing many of these bats in the region remain undocumented. Specifically, we aimed to (i) use morphological characteristics and DNA barcoding to distinguish and identify the bat fly species found on 11 cavernicolous bat species in Hong Kong; (ii) determine the phylogenetic relationships among the bat fly species; (iii) elucidate the patterns of bat-bat fly association and evaluate the degrees of specificity of each bat fly species to their host species. In addition, we also (iv) assemble the mitogenomes of one nycteribiid and two streblid species from different genera to enrich the limited genetic resources available from bat flies for assisting primer design and species identification in future studies. Our findings will provide new knowledge on the species richness of bat flies and the bat-bat fly association network, reflecting the ecological and coevolutionary relationships among bat and bat flies, in an understudied region of unneglectable public health concern.
Methods
Specimen collection
Bats roosting in abandoned mines, water tunnels, and culverts in Hong Kong were captured by hand-held hoop nets during 2018–2022. To avoid inter-host contamination of bat flies, only one bat was kept in each sterilized cloth bag. Each bat was identified to species by morphology [39]. Miniopterus magnater and Miniopterus fuliginosus were morphologically similar, so we used a sterilized wing punch tool to collect 5-mm tissues from the wing membranes for DNA barcoding to confirm their species identity [40]. Bat flies were collected from each bat using a blunt-end forceps. All bats were released back into the wild after sample collection. All samples were immediately preserved in absolute ethanol in the field and stored at −20 °C on the same day until microscopic examination or DNA extraction.
Microscopic examination and DNA barcoding
We examined and photographed all bat fly specimens using a compound microscope (Leica M205 C, Wetzlar, Germany). We separated the bat flies based on their sex and identified the species in each sex based on their morphologies [31,32,33]. A subset of the specimens from each morphospecies in each sex of bat fly (120 samples) and all Miniopterus tissues were then proceeded to DNA extraction using the E.Z.N.A. Tissue DNA Kit (Omega bio-tek, Norcross, USA). We DNA barcoded each sample by using the primer pair LCO1490 (forward primer: 5'GGTCAACAAATCATAAAGATATTGG 3') and HCO2198 (reverse primer: 5'TAAACTTCAGGGTGACCAAAAAATCA3') to amplify a 658 bp DNA fragment from the mitochondrial cytochrome c oxidase subunit I gene (COI) [41]. Each DNA barcoding PCR was performed in 30 μl reaction, containing 6 μl 5X GoTaq Flexi Buffer, 0.6 μl 10 mM dNTP Mix, 3.6 μl 25 mM MgCl2, 0.15 μl 5 U/μl GoTaq G2 Flexi DNA Polymerase (Promega, Madison, USA), 1 μl extracted DNA, 0.6 μl of each primer, 6 μl 10% DMSO (Sigma, Burlington, MA, USA), and ultrapure water. Thermal cycling condition of the PCR was 95 °C for 2 min; 35 cycles of 95 °C for 30 s, 56 °C for 30 s and 72 °C for 1 min; and final extension at 72 °C for 5 min. The target sizes of PCR products were confirmed by gel electrophoresis, and the PCR products were sequenced by BGI (Shenzhen, China).
Phylogenetic analysis
Seventy-one COI sequences of bat flies were used for phylogenetic analysis, in which 20 and 51 sequences were obtained from this study and Genbank, respectively. We aligned the sequences with Clustal W [42] algorithm using MEGA 6.06 [43]. We used ModelFinder in IQ-Tree v2.20 [44] to find the best-fit model of nucleotide substitution, which was the General Time-Reversible (GTR) model with gamma-shaped (G) distribution across sites and invariable sites (I). Maximum likelihood (ML) and Bayesian inference (BI) methods were used for the reconstruction of phylogenetic trees. The ML tree was run for 1000 bootstrap replications using IQ-Tree v2.20 [44]. A Markov chain Monte Carlo (MCMC) search was initiated with random trees and run for 2,000,000 generations using MrBayes v3.2.7 [45], with a sampling frequency of every 1000 generations, and the first 25% of samples were discarded as burn-in. Trees were visualized using FigTree v1.4.4 [46]. We calculated the pairwise p-distances using MEGA 6.06 [43]. Identities of bat fly species with distinct COI sequences (> 2% difference) [47] were cross-checked with the morphological descriptions and illustrations presented in the literature [31,32,33].
Host specificity analysis
We estimated the host specificity of each bat fly species by calculating (i) number of bat fly-infested bats of a bat species (Nb); (ii) number of bat flies of a bat fly species found on the bats of a bat species (Ne); (iii) number of bats of a bat species infested with a particular bat fly species (Nib); (iv) specificity index (SI), which is the percentage of total number of bat flies of a single species found on the host bats of a bat species, i.e. SI of bat fly species A = Ne of species A/total number of species A individuals found on all bats × 100 [48].
Mitogenome assembly and annotation
DNA library of 350-bp insert size was prepared for each of the three bat fly species (i.e. nycteribiid Phthiridium sp. A and streblids Brachytarsina amboinensis and Raymondia sp. A). The libraries were sequenced on an Illumina NovaSeq instrument (PE 150Â bp reads) for 8 G per sample at Novogene. We filtered the sequencing reads using fastp [49] and evaluated the quality of filtered reads with FASTQC [50]. Then, we assembled the mitogenome by MIRA v4.0 [51] and MITOBIM.PL v1.6 [52], using a house fly mitogenome (Musca domestica, accession no. NC024855.1) as a reference. We annotated these three mitogenomes using MITOS [53] and GeSeq [54]; then, we checked the annotation manually based on the annotations from the mitogenomes of three close relatives (NC024855.1: Musca domestica, MK896866.1: Paratrichobius longicrus, and MK896865.1: Paradyschiria parvula). The protein translation was corrected according to the BLAST results and the annotations of these three close relatives. The final mitogenome annotations were visualized using OGDRAW [55].
Results
Bat fly identification
We collected 641 bat flies from 271 bats representing 11 bat species in five bat families, i.e. Rhinolophus sinicus, R. affinis, and R. pusillus in Rhinolophidae; Myotis chinensis, M. pilosus, and M. horsfieldii in Vespertilionidae; Miniopterus magnater, M. fuliginosus, and M. pusillus in Miniopteridae; Hipposideros gentilis in Hipposideridae; and Rousettus leschenaultii in Pteropodidae (Table 1). We found 20 bat fly species, with 12 and eight species belonging to Nycteribiidae and Streblidae, respectively (Fig. 1 and Additional file 1: Figs. S1-S20). Regarding the nycteribiids, there were five Nycteribia spp., three Penicillidia spp., two Phthiridium spp., one Basilia sp., and one from a hitherto unknown genus (Nycteribiidae species A) (Fig. 1 and Additional file 1: Figs. S1-S12). These nycteribiids all belong to the subfamily Nycteribiinae. For the streblids, we identified B. amboinensis (Rondani 1878), three Raymondia spp. [31, 32], and four Brachytarsina spp. (Fig. 1 and Additional file 1: Figs. S13-S20). They all belong to the subfamily Brachytarsininae. Species identified at the genus or family levels could be classified to neither species level based on COI nor morphology. Both sexes of each species were found except for five species (Table 1). All streblids found in this study possessed functional wings.
Images of the dorsal views of female bat flies, unless otherwise specified. Bat flies labeled a-l and m-t belong to Nycteribiidae and Streblidae, respectively. a Nycteribia sp. A; b Nycteribia sp. B; c Nycteribia sp. D; d Nycteribia sp. E; e Nycteribia sp. F; f Phthiridium sp. A; g Phthiridium sp. B, male only; h Basilia sp. A; i Penicillidia sp. A; j Penicillidia sp. B; k Penicillidia sp. C; l Nycteribiidae species A, male only, scale bar unavailable; m Raymondia sp. A; n Raymondia sp. B, male only, scale bar unavailable; o Raymondia sp. C, image shown was a protease-digested specimen and abdomen was contracted; p Brachytarsina amboinensis; q Brachytarsina sp. B; r Brachytarsina sp. C; s Brachytarsina sp. D, male; and t Brachytarsina sp. E, male only. Blue scale bar = 1 mm. Refer to Additional file 1: Figs. S1–S20 for the corresponding images of the ventral views of females and the dorsal and ventral views of males
Phylogenetic relationships among the bat fly species
Among the Nycteribia spp. identified, Nycteribia sp. A was the most distantly related to the other four Nycteribia spp., and it grouped with a Nycteribia specimen known from China (p-distance = 0.3%) (Fig. 2; refer to Additional file 1: Fig. S21 for the phylogenetic trees using more conspecifics for each identified species). Nycteribia sp. B clustered with a Nycteribia specimen from Japan (P-distance = 0.3%) identified as Nycteribia allotopa. In a separate clade, another two sequences also identified as N. allotopa clustered with Nycteribia sp. D (P-distance = 2.7–3.7%) and sp. E (P-distance = 5.3–5.5%). Nycteribia sp. E resembled N. allotopa the most among the species of Nycteribia from Hong Kong according to the taxonomic information within Speiser (1901) [33]. Nycteribia sp. F was closely related to a specimen identified as Nycteribia parvula from the Philippines (P-distance = 3.2%) (Fig. 2). Phthiridium sp. A clustered with a specimen identified as Phthiridium hindlei from Japan (P-distance = 2.4%) and a specimen identified as Phthiridium sp. from South Korea (P-distance = 2.7%) to form a well-supported clade. Phthiridium sp. B grouped with an unidentified species of Phthiridium from China (P-distance = 0.8%). In the polyphyletic genus Basilia, Basilia sp. A was closely related to Basilia nana identified from Hungary (P-distance = 4%) (Fig. 2). Penicillidia sp. A grouped with Penicillidia oceanica identified from the Philippines (P-distance = 2.1%) and further clustered with Penicillidia sp. B as a clade. Penicillidia sp. C was sister to this clade. Nycteribiidae species A is sister to the clades of the four aforementioned nycteribiid genera, but its exact genus identity could not be determined (Fig. 2).
Phylogenetic relationships among bat fly species. The phylogenetic tree was inferred by maximum likelihood (ML) and Bayesian inference (BI) methods based on COI gene (658 bp). The values at each node represent the Bayesian posterior probability and the ML bootstrap value. A dash (–) indicates that the topologies of the ML tree and BI tree do not coincide at that branch and only the ML bootstrap value is shown. The vertical lines on the right indicate the genera or families of these bat fly species
All streblid species identified belong to the Old World Streblidae, which is a paraphyletic group clustered with Nycteribiidae [9] (Fig. 2). Raymondia sp. B and sp. C were highly divergent (P-distance = 7.9%), and they grouped together and formed a clade with Raymondia sp. A. The clade of Raymondia spp. was sister to a clade of two species of Streblidae from Uganda (P-distance = 15.4–18%; Fig. 2). These two sister clades formed a monophyletic group with Nycteribiidae instead of other Old World Streblidae. Raymondia sp. C highly resembled Raymondia pagodarum (Speiser 1900) based on morphology [31], but no COI sequence of Raymondia species was available in GenBank. Brachytarsina sp. E was closely related to an unidentified specimen of Brachytarsina sp. from South Korea (P-distance = 1.7%). Brachytarsina amboinensis grouped with Brachytarsina kanoi in Japan (P-distance = 2%) as a clade and clustered with the clade of Brachytarsina sp. B and sp. D (p-distance between B and D = 3%). No COI sequence of B. amboinensis was available in GenBank. Brachytarsina sp. B and sp. D were superficially similar to Brachytarsina werneri (Jobling 1951) but slight morphological differences could be observed [32]. For example, the two lateral parts of the seventh tergite present in female B. werneri were not observed in Brachytarsina sp. B and sp. D. Brachytarsina sp. C is the most divergent from the four aforementioned Brachytarsina species (Fig. 2).
Patterns of bat-bat fly association
The bat fly species that exhibited the highest host specificity (SI = 100, Fig. 3, and Table 1) included nycteribiid Phthiridium sp. A, Nycteribia sp. A, Nycteribia sp. F, and streblid Raymondia sp. A. Remarkably, each of these bat fly species was highly specific to a single bat species in a different family, i.e. Phthiridium sp. A (n = 128) on R. sinicus in Rhinolophidae; Raymondia sp. A (n = 55) on H. gentilis in Hipposideridae; Nycteribia sp. A (n = 23) on M. pilosus in Vespertilionidae; and Nycteribia sp. F (n = 7) on M. pusillus in Miniopteridae. Nycteribia sp. E also showed high host specificity (SI = 92.86, n = 13) to M. pusillus (Fig. 3 and Table 1).
Host-ectoparasite association network. Web of interactions between bat fly species (right) and bat species (left). The width of bars is proportional to the number of bat fly individuals. Names of species that belong to the same genus are in the same color. Bat photos©Agriculture, Fisheries and Conservation Department
Despite small sample sizes, two bat fly species were the only species associated with their host bat species, so these bat-bat fly relationships were also highly specific. They were nycteribiid Basilia sp. A (SI = 100, n = 2) and streblid Brachytarsina sp. C (SI = 100, n = 3), which were found on M. horsfieldii and R. leschenaultii, respectively (Fig. 3 and Table 1).
Streblid Brachytarsina spp. were detected on nine of the 11 bat species surveyed (except M. horsfieldii and R. pusillus). Brachytarsina amboinensis was prevalent (38% of total bat flies) and it showed the lowest host specificity (SI = 0.41–39.02) among all bat fly species, being found on six bat species in three families. It was abundant on Miniopterus but absent from Rhinolophus species. Other bat fly species with relatively low host specificity included Brachytarsina sp. B (SI = 3.84–61.54), which occurred on three bat species in two families such as Rhinolophus species, as well as nycteribiid Penicillidia sp. C (SI = 6.9–51.72), which was specific to Miniopterus but found on all three Miniopterus spp. (Fig. 3 and Table 1).
Mitogenomes of three bat fly species
The total number of mapped reads of Phthiridium sp. A, B. amboinensis, and Raymondia sp. A were 2029937, 891049, and 492479, respectively. The complete mitogenomes of Phthiridium sp. A, B. amboinensis, and Raymondia sp. A showed slight difference in sizes (Fig. 4). The total lengths of these three mitogenomes were 16155 bp, 16480 bp, and 16514 bp, respectively. These mitogenome sizes were very similar to those of other calyptrates (14–16 k bp), e.g. Musca domestica is 16108 bp, Paratrichobius longicrus is 16296 bp, and Paradyschiria parvula is 14588 bp [56, 57]. There are 37 genes within each mitogenome, including 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), and one non-coding control region (CR) (Fig. 4). The organization and structures of genes in these three mitogenomes were identical, with 23 genes (including nine PCGs and 14 tRNAs) encoded on the heavy strand and 14 genes (including four PCGs, eight tRNAs and two rRNAs) encoded on the light strand.
Mitogenomes of a Phthiridium sp. A, b Raymondia sp. A, and c Brachytarsina amboinensis. The genes labeled inside and outside of the cycle were encoded on the light and heavy strand, respectively. Genes in the same color code belong to the same gene family. The gray arrows indicate the direction of gene transcription. The grey circle in the middle shows the GC content of the mitogenome
Discussion
Both evolutionary and ecological factors were suggested to be important for shaping the patterns of host association of bat flies, and some previous studies examined the significance of either one or both factors in explaining these patterns [58,59,60]. These studies demonstrated that bat flies generally showed variable degrees of host specialization and low cophylogenetic congruence to their hosts, indicating that extant patterns of bat-bat fly association may not be contributed by cospeciation. Remarkably, the bat-bat fly association unveiled in this study clearly showed that certain closely related bat flies within Nycteribiidae and Streblidae had affinities toward particular host bat species that are phylogenetically more closely related. For example, we observed that the congenerics of Nycteribia and conspecifics of B. amboinensis only infested hosts in Myotis and Miniopterus, which belong to two closely related families in Vespertilionoidea [61], whereas congenerics of Raymondia only parasitized hosts in Rhinolophus and Hipposideros, which are in two closely related families in Rhinolophoidea. These bat fly-bat genus associations observed were also supported by similar host associations of Nycteribia spp., B. amboinensis, and Raymondia spp. revealed in other nearby regions, such as Thailand and Malaysia [62, 63].
From generalist to specialist: Bat-bat fly association
Elucidating the true distribution patterns of bat flies in a community of host bats is crucial for understanding the complex ecology of bat fly parasitism. With recent carefully controlled bat fly surveys, volant streblids in the Neotropics were reported as highly specialized [48, 64], with most species being monoxenous (i.e. infesting only a single host species). Less specialized species were also mainly associated with their primary host species [21, 65]. In this study, we found both generalist and specialist streblids that are monoxenous, stenoxenous (i.e. infesting two or more congeneric host species), or polyxenous (i.e. infesting two or more host genera) [65]. Recent records of B. amboinensis were mostly reported from the Philippines where the species infested multiple host species in Miniopterus, Myotis, Rhinolophus, and Hipposideros [66, 67]. However, according to older records, B. amboinensis also occurred in Taiwan and Japan, and those in Japan infested M. fuliginosus [68, 69]. Likewise, we found local B. amboinensis to be polyxenous, with specificity toward different host genera being unequal. About 77% B. amboinensis were associated with M. magnater and M. pusillus, whereas 22% parasitized M. pilosus and M. chinensis. Considering its flight capability, it was possible that the low host specificity was due to natural host switches or, alternatively but unlikely, random transfers [21]. Three other closely related Brachytarsina spp. were morphologically similar to B. amboinensis. However, unlike B. amboinensis, they appeared either monoxenous or stenoxenous. Over 96% of Brachytarsina sp. B were associated with R. affinis and R. sinicus, and Brachytarsina sp. D and sp. E were only found on R. sinicus. Thus, B. amboinensis did not share the same host species and specificity with other closely related Brachytarsina species. Notably, many individuals of other closely related Brachytarsina spp. and B. amboinensis were isolated from hosts captured in the same roosts on the same sampling dates, but only B. amboinensis showed this generalized association with several Miniopterus and Myotis species but not Rhinolophus. Thus, it is likely that B. amboinensis is a generalist parasite and exhibited natural dispersal between bat host species, predominantly Miniopterus and Myotis.
In addition, we found three streblid species in genus Raymondia. Raymondia sp. A was strictly monoxenous and only parasitized H. gentilis. Almost no other bat fly species and no nycteribiid were found on H. gentilis. The monoxeny of Raymondia sp. A could be in part attributed to their roosting behavior. Hipposideros gentilis often co-roost with other host species in caves, such as R. pusillus, but individuals of H. gentilis in colonies usually maintain certain interbat spacing and guard their area against intrusion [39], which might lessen inter-host switching of streblids. Moreover, the mobility of Raymondia spp. might be relatively limited by their tiny body sizes as Raymondia species were the smallest (< 1.5 mm) among all bat flies identified [31]. For Raymondia spp. B and C, we only secured one individual of each of these species, which infested R. pusillus and R. affinis, respectively. Raymondia sp. C is morphologically highly similar to R. pagodarum, which were found to mainly parasitize Hipposideros spp. and Rhinolophus spp. in Asian regions [70, 71]. Hipposideros gentilis was also one of these host species that R. pagodarum infested in Thailand [63]. Raymondia pagodarum and another species, Raymondia molossia, were also reported in China, but their hosts were unknown [31, 69].
In this study, we found more species in Nycteribiidae than Streblidae, which is consistent with the biogeographic pattern that Nycteribiidae species are primarily found in the Old World [10]. Compared to other identified nycteribiid genera, Nycteribia was the most species-rich group with five species found locally. Nycteribia sp. A was closely related to a Nycteribia sp. found in Hubei, China, whose primary host was unknown [72]. We found that Nycteribia sp. A was the only Nycteribia spp. we identified that parasitized local Myotis spp., and it was monoxenous and only infested M. pilosus. In Hong Kong, even though individuals of M. pilosus commonly mix with those of Miniopterus spp. and M. chinensis to form packed aggregations in roosts [39], and this roosting structure likely facilitates interspecific host exchanges of bat flies, Nycteribia sp. A was absent from other host species. Nycteribia spp. in proximate regions, including Nycteribia quasiocellata found in Manchuria in China, Mongolia, and Kazakhstan and a Nycteribia sp. in Thailand, were other congenerics that infested Myotis petax and Myotis siligorensis, respectively [63, 73].
In the phylogenetic tree, two divergent clades in Nycteribia contain the GenBank sequences identified as N. allotopa; thus, some N. allotopa in the tree were likely misidentified. Nycteribia sp. B was identical to a Nycteribia species claimed to be N. allotopa in Wakayama, Japan, associated with M. fuliginosus [74]. Nycteribia allotopa was also reported in Taiwan, Korea, and Thailand where they also lived on M. fuliginosus [63, 68, 75, 76]. In Hong Kong, there are three sympatric Miniopterus species, including M. magnater, M. pusillus, and M. fuliginosus, which are morphologically similar. Individuals of M. magnater or M. fuliginosus occasionally form tightly packed and mixed assemblages with those of M. pusillus, but co-roosting of M. magnater and M. fuliginosus is locally rare (AFCD unpublished data). Our results indicated that Nycteribia sp. B was stenoxenous and parasitized all three Miniopterus species; it was the only Nycteribia found on M. fuliginosus. Notably, the distribution ranges of M. magnater and M. pusillus do not include Japan, Korea, and Taiwan [77]. Moreover, N. allotopa was also reported to be found on other host species that were absent from Hong Kong, such as other species in Miniopterus, Rhinolophus, Pipistrellus, Megaderma, and Tadarida in nearby regions [71, 78,79,80]. These records might reflect the potential of Nycteribia sp. B in inter-host switching. The other two Nycteribia spp., Nycteribia sp. D and sp. E, were closely related to the Nycteribia that described as N. allotopa in Wakayama, Japan [81]. Yet unlike N. allotopa, Nycteribia sp. D and sp. E were not found on M. fuliginosus but predominantly infested M. magnater and M. pusillus, respectively.
Another identified nycteribiid genus in which the congenerics were exclusively associated with Miniopterus was Penicillidia. We found that Penicillidia spp. A, B, and C were all stenoxenous. Penicillidia sp. A was closely related to P. oceanica in the Philippines which infested Miniopterus schreibersi [82]. It parasitized both M. magnater and M. fuliginosus that rarely co-roost in Hong Kong. Penicillidia sp. B was associated with M. magnater and M. pusillus, which often co-roost. Penicillidia sp. C were also found on M. fuliginosus. Other congeneric species, e.g. Penicillidia jenynsii in Japan, also infested Miniopterus species [68]. Penicillidia monoceros was reported to occur in Hubei in China and Mongolia, but those in Mongolia were reported to primarily infest Myotis petax [72, 73]. Notably, we observed that Miniopterus spp. hosted the most diverse communities of bat fly species, with the number of species ranging from four to seven. Contrary to other bat genera, the bat fly species assemblages among the three congenerics of Miniopterus largely overlapped with each other, and they shared at least three bat fly species, including B. amboinensis, Nycteribia sp. B, and Penicillidia sp. C. Due in part to the gregarious habits of Miniopterus species and their propensity to form tightly packed clusters in caves, Miniopterus individuals offered various opportunities to horizontally transfer their bat flies, and these bat flies could find mates readily on multiple host species to reproduce, so adapting and exploiting tightly packed hosts in multiple congenerics should increase the abundance and overall fitness of these bat flies [18]. Slight intra-specific morphological variations were also observed within populations of Penicillidia spp. B and C; it was proposed that phenotypic plasticity of an ectoparasitic species might confer greater fitness advantage in their ability to parasitize a wider range of host species [58].
While Penicillidia was found on Miniopterus exclusively, identified species in Phthiridium were exclusively associated with Rhinolophus. Phthiridium sp. A was a close relative to P. hindlei found in Osaka, Japan, that lived on Rhinolophus ferrumequinum [81]. Records of other examples of Phthiridium spp. in China included P. hindlei infesting R. ferrumequinum in Shandong, P. szechuanum found on R. pusillus in Sichuan, and P. ornatum parasitizing Rhinolophus sp. in Yunnan [25, 69, 83]. Although Phthiridium sp. A was very abundant on R. sinicus and individuals of R. sinicus can form packed and mixed assemblages with those of R. affinis in Hong Kong, especially during winter, Phthiridium sp. A was strictly monoxenous. It was also the only nycteribiid species found on R. sinicus, even though R. sinicus co-roosts with M. pusillus and M. pilosus, for example, which harbor other nycteribiids. Dispersal limitations, adaptative limitations, and reproductive isolation were several main explanations previously proposed to broadly account for how high host specificity of bat flies might evolve and be maintained [18]. However, mechanisms underlying bat-bat fly relationships are complex, and most research on our studied bat fly genera mainly focused on host-parasite occurrences and interactions; studies dealing with the ecology and biology of these studied genera and species in detail were scarce. It remains of interest to investigate which factors are involved and how they might control the host specificity of these bat fly species.
Challenges and future directions
On a final note, we would like to highlight the challenge of species identification based on old taxonomic keys [31, 33, 68, 70], especially for regions with limited prior information on the bat fly community. Moreover, cryptic species and morphological plasticity are likely to be common in bat flies [29, 84, 85], making existing taxonomic keys insufficient for accurate species identification. In this study, most bat flies were morphologically distinct except a few cryptic species that were challenging to differentiate. For example, Brachytarsina spp. B, D, and E were morphologically highly similar and shared the same hosts, but they were differentiated by genetic divergence. Slight variations in morphology were observed among conspecifics of Penicillidia spp. B and C, respectively, but revealed to be intraspecific variations by DNA barcoding results. Therefore, as suggested by some recent studies [13, 37], we advocate identifying bat fly species using morphological characteristics with the support of DNA barcoding to discern bat-bat fly associations accurately in future studies. In addition to genetic data, provision of high-quality photos and formal species description with the development of new taxonomic keys will be crucial resources for efficient bat fly classification, which are especially important for understudied regions potentially with many undescribed species.
Conclusions
In this study, we found 20 bat fly species from a subtropical region in East Asia in which many of them appear to be new records. We have also unveiled the associations of these bat fly species to host bat species and revealed a range of host specificity among these bat flies. However, detailed information on the biology and ecology of bat flies, as well as host bat species, occurring in the East Asia region remains limited, which makes elucidation of the complex mechanisms underlying bat-bat fly associations difficult. More studies on bat flies and bats in the region, such as from bat fly species discovery to their distribution and behaviors on hosts, will be essential to better understand how various evolutionary or ecological factors shape the extant bat-bat fly relationships in the regions.
Availability of data and materials
The GenBank accession numbers of the bat fly DNA sequences and mitogenomes are OQ184520–184639 and OQ301747–301749, respectively.
References
Scott M, Dobson A. The role of parasites in regulating host abundance. Parasitol Today. 1989;5:176–83.
Tompkins D, Dobson A, Arneberg P, Begon M, Cattadori I, Greenman J, et al. Parasites and host population dynamics. The ecology of wildlife diseases. 2002; 45–62.
Thomas F, Poulin R, Brodeur J. Host manipulation by parasites: a multidimensional phenomenon. Oikos. 2010;119:1217–23.
Reeves WK, Lloyd JE. Louse flies, keds, and bat flies (Hippoboscoidea). Medical and veterinary entomology. Amsterdam: Elsevier; 2019. p. 421–38.
Poinar G, Brown A. The first fossil streblid bat fly, Enischnomyia stegosoma ng, n. sp. (Diptera: Hippoboscoidea: Streblidae). Syst Parasitol. 2012;81:79–86.
Haelewaters D, Hiller T, Dick CW. Bats, bat flies, and fungi: a case of hyperparasitism. Trends Parasitol. 2018;34:784–99.
Graciolli G, Dick C. Checklist of world Nycteribiidae (Diptera: Hippoboscoidea). 2008.
Dick C, Graciolli G. Checklist of world Streblidae (Diptera: Hippoboscoidea). 2009.
Dittmar K, Porter ML, Murray S, Whiting MF. Molecular phylogenetic analysis of nycteribiid and streblid bat flies (Diptera: Brachycera, Calyptratae): implications for host associations and phylogeographic origins. Mol Phylogenet Evol. 2006;38:155–70.
Dick CW, Patterson BD. Bat flies: obligate ectoparasites of bats. Micromammals and macroparasites. Berlin: Springer; 2006. p. 179–94.
Ter Hofstede HM, Fenton MB. Relationships between roost preferences, ectoparasite density, and grooming behaviour of neotropical bats. J Zool. 2005;266:333–40.
Patterson BD, Dick CW, Dittmar K. Roosting habits of bats affect their parasitism by bat flies (Diptera: Streblidae). J Trop Ecol. 2007;23:177–89.
Hiller T, Vollstädt MG, Brändel SD, Page RA, Tschapka M. Bat–bat fly interactions in Central Panama: host traits relate to modularity in a highly specialised network. Insect Conserv Divers. 2021;14:686–99.
Fagundes R, Antonini Y, Aguiar LM. Overlap in cave usage and period of activity as factors structuring the interactions between bats and ectoparasites. Zool Stud. 2017;56:e22.
Szentiványi T, Vincze O, Estók P. Density-dependent sex ratio and sex-specific preference for host traits in parasitic bat flies. Parasit Vectors. 2017;10:1–9.
PatrÃcio PMP, Lourenço EC, Freitas AQD, Famadas KM. Host morphophysiological conditions and environment abiotic factors correlate with bat flies (Streblidae) prevalence and intensity in Artibeus Leach, 1821 (Phyllostomidae). Ciência Rural. 2016;46:648–53.
Lee VN, Mendenhall IH, Lee BP-H, Posa MRC. Parasitism by bat flies on an urban population of Cynopterus brachyotis in Singapore. Acta Chiropterologica. 2018;20:177–85.
Dick CW, Patterson BD. Against all odds: explaining high host specificity in dispersal-prone parasites. Int J Parasitol. 2007;37:871–6.
Reckardt K, Kerth G. Does the mode of transmission between hosts affect the host choice strategies of parasites? Implications from a field study on bat fly and wing mite infestation of Bechstein’s bats. Oikos. 2009;118:183–90.
Zarazúa-Carbajal M, Saldaña-Vázquez RA, Sandoval-Ruiz CA, Stoner KE, Benitez-Malvido J. The specificity of host-bat fly interaction networks across vegetation and seasonal variation. Parasitol Res. 2016;115:4037–44.
Dick CW. High host specificity of obligate ectoparasites. Ecol Entomol. 2007;32:446–50.
Patterson BD, Dick CW, Dittmar K. Nested distributions of bat flies (Diptera: Streblidae) on Neotropical bats: artifact and specificity in host-parasite studies. Ecography. 2009;32:481–7.
Ter Hofstede HM, Fenton MB, Whitaker J, John O. Host and host-site specificity of bat flies (Diptera: Streblidae and Nycteribiidae) on Neotropical bats (Chiroptera). Can J Zool. 2004;82:616–26.
Wang X, Zhou R, Lu L, Wang C, Liu Q. A New Record of Ornithoica aequisenta and an Updated Checklist of Hippoboscidae, Nycteribiidae, and Streblidae in China. J Med Entomol. 2022;59:1071–5.
Satô M, Mogi M. A new species of Phthiridium (Diptera: Nycteribiidae) from Iriomote Island, the Ryukyu Islands, Japan, with a key to nycteribiid bat flies of Japan. Med Entomol Zool. 2015;66:1–6.
Riley S, Fraser C, Donnelly CA, Ghani AC, Abu-Raddad LJ, Hedley AJ, et al. Transmission dynamics of the etiological agent of SARS in Hong Kong: impact of public health interventions. Science. 2003;300:1961–6.
Tian H, Liu Y, Li Y, Wu C-H, Chen B, Kraemer MU, et al. An investigation of transmission control measures during the first 50 days of the COVID-19 epidemic in China. Science. 2020;368:638–42.
Brook CE, Dobson AP. Bats as ‘special’reservoirs for emerging zoonotic pathogens. Trends Microbiol. 2015;23:172–80.
Dick CW, Dittmar K. Parasitic bat flies (Diptera: Streblidae and Nycteribiidae): host specificity and potential as vectors. In: Klimpel S, Mehlhorn H, editors. Bats (Chiroptera) as vectors of diseases and parasites. Berlin: Springer; 2014. p. 131–55.
Morse SF, Olival KJ, Kosoy M, Billeter S, Patterson BD, Dick CW, et al. Global distribution and genetic diversity of Bartonella in bat flies (Hippoboscoidea, Streblidae, Nycteribiidae). Infect Genet Evol. 2012;12:1717–23.
Jobling B. A revision of the genus Raymondia Frauenfeld (Diptera pupipara, Streblidae). Parasitology. 1930;22:283–301.
Jobling B. A record of the Streblidae from the Philippines and other Pacific Islands, including morphology of the abdomen, host-parasite relationship and geographical distribution, and with descriptions of five new species (Diptera). Trans Royal Entomol Soc Lond. 1951;102:211–46.
Theodor O. Illustrated catalogue of the Rothschild collection of Nycteribiidae (Diptera) in the British Museum (Natural History); with keys and short descriptions for the identification of subfamilies, genera, species and subspecies. London and Tonbridge: The Whitefriars Press Ltd. 1967.
Theodor O. Philippine batflies of the family Nycteribiidae (Diptera: Pupipara). 1963.
Cardoso P, Erwin TL, Borges PA, New TR. The seven impediments in invertebrate conservation and how to overcome them. Biol Cons. 2011;144:2647–55.
Brito D. Overcoming the Linnean shortfall: data deficiency and biological survey priorities. Basic Appl Ecol. 2010;11:709–13.
Pejić B, Budinski I, van Schaik J, Blagojević J. Sharing roosts but not ectoparasites: high host-specificity in bat flies and wing mites of Miniopterus schreibersii and Rhinolophus ferrumequinum (Mammalia: Chiroptera). Curr Zool. 2021. https://doi.org/10.1093/cz/zoab086.
Hong Kong Biodiversity Information Hub. Agriculture, Fisheries and Conservation Department, Hong Kong. 2022. https://bih.gov.hk/tc/hong-kong-species/mammals/index.html. Accessed 6 Oct 2022.
Shek CT. Field guide to the terrestrial mammals of Hong Kong. Hong Kong: Cosmos Books; 2006.
Kunz T, Parsons S. Ecological and behavioral methods for the study of bats. Baltimore: Johns Hopkins University Press; 2009.
Vrijenhoek R. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol. 1994;3:294–9.
Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994;22:4673–80.
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol. 2013;30:2725–9.
Nguyen L-T, Schmidt HA, Von Haeseler A, Minh BQ. IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol Biol Evol. 2015;32:268–74.
Ronquist F, Huelsenbeck JP. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003;19:1572–4.
Rambaut A. FigTree v1.4.4, a graphical viewer of phylogenetic trees. 2018. http://tree.bio.ed.ac.uk/software/figtree/. Accessed 3 Aug 2022.
Hebert PD, Ratnasingham S, De Waard JR. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proc Royal Soc London Ser B Biol Sci. 2003;270:S96–9.
Dick CW, Gettinger D. A faunal survey of streblid flies (Diptera: Streblidae) associated with bats in Paraguay. J Parasitol. 2005;91:1015–24.
Chen S, Zhou Y, Chen Y, Gu J. fastp: an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics. 2018;34:i884–90.
FastQC: a quality control tool for high throughput sequence data. Babraham Bioinformatics, Babraham Institute, Cambridge, United Kingdom. 2010. https://www.bioinformatics.babraham.ac.uk/projects/fastqc/. Accessed 21 Sep 2022.
Chevreux B, Wetter T, Suhai S, editors. Genome sequence assembly using trace signals and additional sequence information. German conference on bioinformatics. Princeton: Citeseer; 1999.
Hahn C, Bachmann L, Chevreux B. Reconstructing mitochondrial genomes directly from genomic next-generation sequencing reads—a baiting and iterative mapping approach. Nucleic Acids Res. 2013;41:e129.
Bernt M, Donath A, Jühling F, Externbrink F, Florentz C, Fritzsch G, et al. MITOS: improved de novo metazoan mitochondrial genome annotation. Mol Phylogenet Evol. 2013;69:313–9.
Tillich M, Lehwark P, Pellizzer T, Ulbricht-Jones ES, Fischer A, Bock R, et al. GeSeq–versatile and accurate annotation of organelle genomes. Nucleic Acids Res. 2017;45:W6–11.
Greiner S, Lehwark P, Bock R. OrganellarGenomeDRAW (OGDRAW) version 1.3.1: expanded toolkit for the graphical visualization of organellar genomes. Nucleic Acids Res. 2019;47:W59–64.
Li X, Wang Y, Su S, Yang D. The complete mitochondrial genomes of Musca domestica and Scathophaga stercoraria (Diptera: Muscoidea: Muscidae and Scathophagidae). Mitochondrial DNA Part A. 2016;27:1435–6.
Trevisan B, Alcantara DM, Machado DJ, Marques FP, Lahr DJ. Genome skimming is a low-cost and robust strategy to assemble complete mitochondrial genomes from ethanol preserved specimens in biodiversity studies. PeerJ. 2019;7:e7543.
Brown AM, Speer KA, Teixeira T, Clare E, Simmons NB, Balbuena JA, et al. Phylogenetic and ecological trends in specialization: disentangling the drivers of ectoparasite host specificity. https://doi.org/10.1101/2022.04.06.487338.
Nikoh N, Kondo N, Fukatsu T. Phylogenetic comparison between nycteribiid bat flies and their host bats. Med Entomol Zool. 2011;62:185–94.
Graciolli G, de Carvalho CJ. Do fly parasites of bats and their hosts coevolve? Speciation in Trichobius phyllostomae group (Diptera, Streblidae) and their hosts (Chiroptera, Phyllostomidae) suggests that they do not. Revista Brasileira de Entomologia. 2012;56:436–50.
Amador LI, Moyers Arévalo RL, Almeida FC, Catalano SA, Giannini NP. Bat systematics in the light of unconstrained analyses of a comprehensive molecular supermatrix. J Mamm Evol. 2018;25:37–70.
Azhar I, Khan FAA, Ismail N, Abdullah M. Checklist of bat flies (Diptera: Nycteribiidae and Streblidae) and their associated bat hosts in Malaysia. Check List. 2015;11:1777.
Samoh A, Pantip V, Soisook P. A checklist of Nycteribiid and Streblid Bat Flies (Diptera: Nycteribiidae and Streblidae) from Thailand with thirteen new records for the country. Trop Nat Hist. 2021;21:244–62.
Presley SJ. Ectoparasitic assemblages of Paraguayan bats: ecological and evolutionary perspectives. Lubbock: Texas Tech University; 2004.
Wenzel RL, Tipton VJ. Some relationships between mammal hosts and their ectoparasites. In: Wenzel RL, Tipton VJ, editors. Ectoparasites of Panama. Chicago: Field Museum of Natural History; 1966. p. 405–675.
Obdianela MCN, Guanlao M, Samaniego EVE, Pornobi KO. Prevalence and host specificity of bat flies (Streblidae) from selected caves in Unisan, Quezon. Philippines Acta Parasitologica. 2021;66:983–8.
Alvarez JD, Lit IL, Alviola PA, Cosico EA, Eres EG. A contribution to the ectoparasite fauna of bats (Mammalia: Chiroptera) in Mindoro Island, Philippines: I. Blood sucking Diptera (Nycteribiidae, Streblidae) and Siphonaptera (Ischnopsyllidae). Int J Trop Insect Sci. 2016;36:188–94.
Maa T. A synopsis of Diptera Pupipara of Japan. Pac Insects. 1967;9:727–60.
Zhang D, Li XY, Pei WY. Species catalogue of China, volume 2. Animals. Insecta (VII). Diptera (3): Cyclorrhaphous Brachycera (I). Beijing: Science Press; 2020.
Maa T. Records and descriptions of Nycteribiidae and Streblidae (Diptera). Pacific Insects. 1962;4:417–36.
Hill JE, McNeely JA. The Bats and Bat's Parasites of Thailand. Applied Scientific Research Corporation of Thailand Bangkok; 1975.
Han HJ, Li ZM, Li X, Liu JX, Peng QM, Wang R, et al. Bats and their ectoparasites (Nycteribiidae and Spinturnicidae) carry diverse novel Bartonella genotypes, China. Transboundary Emerg Dis. 2022;69:e845–58.
Sceffler I, Dolch D, Ariunbold J, Batsikhan N, Abraham A, Thiele K. Ectoparasites of bats in Mongolia (Ischnopsyllidae, Nycteribiidae, Cimicidae and Spinturnicidae). Erforsch. Biol. Ress. Mongolei (Halle/Saale). 2010; 11:367-81.
Nabeshima K, Sato S, Kabeya H, Komine N, Nanashima R, Takano A, et al. Detection and phylogenetic analysis of Bartonella species from bat flies on eastern bent-wing bats (Miniopterus fuliginosus) in Japan. Comp Immunol Microbiol Infect Dis. 2020;73:101570.
Tai YL, Lee Y-F, Kuo Y-M, Kuo Y-J. Effects of host state and body condition on parasite infestation of bent-wing bats. Front Zool. 2022;19:1–13.
Lee H, Seo M-G, Lee S-H, Oem J-K, Kim S-H, Jeong H, et al. Relationship among bats, parasitic bat flies, and associated pathogens in Korea. Parasit Vectors. 2021;14:1–11.
The IUCN Red List of Threatened Species. Version 2022-1. IUCN Global Species Programme Red List Unit, Cambridge, United Kingdom. 2022. https://www.iucnredlist.org. Accessed 24 Sep 2022.
Amarga AKS, Alviola PA, Lit IL Jr, Yap SA. Checklist of ectoparasitic arthropods among cave-dwelling bats from Marinduque Island, Philippines. Check List. 2017;13:2029.
Kim HC, Han SH, Dick CW, Choi YG, Chong ST, Klein TA, et al. Geographical distribution of bat flies (Diptera: Nycteribiidae and Streblidae), including two new records, Nycteribia allotopa and N. formosana, collected from bats (Chiroptera: Rhinolophidae and Vespertilionidae) in the Republic of Korea. J Vector Ecol. 2012;37:333–7.
Satô M, Mogi M. Records of some blood-sucking flies from birds and bats of Japan (Diptera: Hippoboscidae, Nycteribiidae and Streblidae). Rishiri Stud. 2008;27:41–8.
Hosokawa T, Nikoh N, Koga R, Satô M, Masahiko T, Meng X-Y, et al. Reductive genome evolution, host–symbiont co-speciation and uterine transmission of endosymbiotic bacteria in bat flies. ISME J. 2012;6:577–87.
Tortosa P, Dsouli N, Gomard Y, Ramasindrazana B, Dick CW, Goodman SM. Evolutionary history of Indian Ocean nycteribiid bat flies mirroring the ecology of their hosts. PLoS ONE. 2013;8:75215.
Maa T-c. Genera and species of Hippoboscidae (Diptera): types, synonymy, habitats and natural groupings. Pac Insects Monogr. 1963;6:1–186.
Smith MA, Woodley NE, Janzen DH, Hallwachs W, Hebert PD. DNA barcodes reveal cryptic host-specificity within the presumed polyphagous members of a genus of parasitoid flies (Diptera: Tachinidae). Proc Natl Acad Sci. 2006;103:3657–62.
Whiteman NK, Sánchez P, Merkel J, Klompen H, Parker PG. Cryptic host specificity of an avian skin mite (Epidermoptidae) vectored by louseflies (Hippoboscidae) associated with two endemic Galapagos bird species. J Parasitol. 2006;92:1218–28.
Acknowledgements
We thank Chi Pan Tong and Alex Wai Kit Lo from the AFCD of Hong Kong SAR Government and Karen Ka Lam Yeung for their assistance during our study. The computations were performed using the research computing facilities offered by the Information Technology Services, the University of Hong Kong.
Funding
This research was funded by the Seed Funding for Strategic Interdisciplinary Research Scheme 2021/22 by the University of Hong Kong.
Author information
Authors and Affiliations
Contributions
ESKP and SYWS designed research; ESKP, GC, HYT, CTS, TWC, and SYWS performed research; ESKP and GC analyzed data; BG and HZ provided advice on research; ESKP wrote the manuscript. All authors read and approved the final manuscript.
Corresponding author
Ethics declarations
Ethics approval and consent to participate
Approvals for animal experiments were granted by the Department of Health (ref. 22–39 in DH/HT&A/8/2/3 Pt.38), the University of Hong Kong (ref. 6043–22), the Agriculture, Fisheries, and Conservation Department (ref. 35 in AF GR CON 09/51 Pt.8), and the Water Department. All authors consent to participate in this study.
Consent for publication
Not applicable.
Competing interests
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential competing interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Additional file 1: Figure S1.
Images of Nycteribia sp. A: (a) dorsal and (b) ventral views of male and (c) dorsal and (d) ventral views of female. Scale bar = 1 mm. Figure S2. Images of Nycteribia sp. B: (a) dorsal and (b) ventral views of male and (c) dorsal and (d) ventral views of female. Scale bar = 1 mm. Figure S3. Images of Nycteribia sp. D: (a) dorsal and (b) ventral views of male and (c) ventral and (d) dorsal views of female. Scale bar = 1 mm. Figure S4. Images of Nycteribia sp. E: (a) dorsal and (b) ventral views of male and (c) ventral and (d) dorsal views of female. Scale bar = 1 mm. Figure S5. Images of Nycteribia sp. F: (a) dorsal and (b) ventral views of male and (c) dorsal and (d) ventral views of female. Scale bar = 1 mm. Figure S6. Images of Phthiridium sp. A: (a) dorsal and (b) ventral views of male and (c) dorsal and (d) ventral views of female. Scale bar = 1 mm. Figure S7. Images of Phthiridium sp. B: (a) dorsal and (b) ventral views, male only. Scale bar = 1 mm. Figure S8. Images Basilia sp. A: (a) dorsal and (b) ventral views, female only. Scale bar = 1 mm. Figure S9. Images of Penicillidia sp. A: (a) ventral and (b) dorsal views of male and (c) ventral and (d) dorsal views of female. Scale bar = 1 mm. Figure S10. Images of Penicillidia sp. B: (a) dorsal and (b) ventral views of male and (c) dorsal and (d) ventral views of female. Scale bar = 1 mm. Figure S11. Images of Penicillidia sp. C: (a) ventral and (b) dorsal views of male and (c) dorsal and (d) ventral views of female. Scale bar = 1 mm. Figure S12. Images of Nycteribiidae species A: (a) dorsal and (b) ventral views, male only, scale bar unavailable. Figure S13. Images of Raymondia sp. A: (a) dorsal and (b) ventral views of female and (c) dorsal and (d) ventral views of male. Scale bar = 1 mm. Figure S14. Images of Raymondia sp. B: (a) dorsal and (b) ventral views, male only, scale bar unavailable. Figure S15. Images of Raymondia sp. C: (a) and (c) ventral and (b) dorsal views, female only. Scale bar = 1 mm for (a) and (b) and no scale bar available for (c). Images of (a) and (b) shown were captured from a protease-digested specimen and abdomen was contracted. Figure S16. Images of Brachytarsina amboinensis: (a) dorsal and (b) ventral views of male and (c) dorsal and (d) ventral views of female. Scale bar = 1 mm. Figure S17. Images of Brachytarsina sp. B: (a) dorsal and (b) ventral views of male and (c) dorsal and (d) ventral views of female. Scale bar = 1 mm. Figure S18. Images of Brachytarsina sp. C: (a) dorsal and (b) ventral views of female, scale bar = 1 mm; (c) ventral and (d) dorsal views of male, scale bar unavailable. Figure S19. Images of Brachytarsina sp. D: (a) dorsal and (b) ventral views of male and (c) dorsal and (d) ventral views of female. Images of the female shown were captured from a protease-digested specimen. Scale bar = 1 mm. Figure S20. Images of Brachytarsina sp. E: (a) dorsal and (b) ventral views, male only. Scale bar = 1 mm. Figure S21. Phylogenetic relationships of all DNA barcoded individuals of the 20 bat fly species identified in this study, inferred from the (a) Bayesian inference (BI) and (b) maximum likelihood (ML) methods based on the COI gene (609 bp). The values before each node represent the Bayesian posterior probability and the ML bootstrap value.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
About this article
Cite this article
Poon, E.S.K., Chen, G., Tsang, H.Y. et al. Species richness of bat flies and their associations with host bats in a subtropical East Asian region. Parasites Vectors 16, 37 (2023). https://doi.org/10.1186/s13071-023-05663-x
Received:
Accepted:
Published:
DOI: https://doi.org/10.1186/s13071-023-05663-x
Keywords
- Bat parasites
- Ectoparasite
- Host specificity
- Host-parasite coevolution
- Nycteribiidae
- Streblidae