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Table 1 Comparison of the sequence characteristics of PTP1–PTP4 from the genus Encephalitozoon

From: The microsporidian polar tube: origin, structure, composition, function, and application

Protein

Gene symbol

Number of amino acids

pI

Major amino acid

Number of cysteine

Signal peptide

O-glycosylation site

N-glycosylation site

Subcellular location

EcPTP1

ECU06_0250

395

4.5

P

17

NF

Y

Y

On the whole PT of Enc. cuniculi [71]

EiPTP1

Eint_060150

371

4.3

P

17

Y

Y

NF

EhPTP1

EHEL_060170

413

4.2

P

19

Y

Y

Y

ErPTP1

EROM_060160

380

4.4

S

15

NF

Y

Y

EcPTP2

ECU06_0240

277

8.6

K

8

Y

Y

NF

On the whole PT of Enc. cuniculi [82]

EiPTP2

Eint_060140

275

8.6

K

8

Y

Y

Y

EhPTP2

EHEL_060160

272

8.8

K

8

Y

Y

Y

ErPTP2

EROM_060150

274

8.8

K

8

Y

Y

Y

EcPTP3

ECU11_1440

1256

6.1

A

1

Y

Y

Y

On the whole PT of Enc. cuniculi [73]

EiPTP3

Eint_111330

1256

5.3

E

1

Y

Y

Y

EhPTP3

EHEL_111330

1284

6.1

A

1

Y

Y

Y

ErPTP3

EROM_111330

1254

6.5

A

1

Y

Y

Y

EcPTP4

ECU07_1090

276

7.1

E

6

Y

Y

Y

On the tip of the PT of Enc. hellem [35]

EiPTP4

Eint_071050

279

7.3

E

6

NF

Y

Y

EhPTP4

EHEL_071080

278

7.9

E

6

Y

Y

Y

ErPTP4

EROM_071050

280

8.4

E

6

NF

Y

Y

  1. “Ec” = Encephalitozoon cuniculi, “Ei” = Enc. intestinalis, “Eh” = Enc. hellem, “Er” = Enc. romalae, PT = polar tube
  2. “Y” = YES, “NF” = not found
  3. The signal peptide was predicted by SignalP 4.1 (https://services.healthtech.dtu.dk/services/SignalP-4.1/)
  4. NETNGLYC (https://services.healthtech.dtu.dk/services/NetNGlyc-1.0/) and NETOGLYC (https://services.healthtech.dtu.dk/services/NetOGlyc-4.0/) were used to analyze N- and O-glycosylation sites