Skip to main content
Download PDF

Efficacy of an oral formulation of afoxolaner and milbemycin oxime against Tunga penetrans in naturally infected dogs

Parasites & Vectors volume 16, Article number: 446 (2023) Cite this article

Abstract

Background

The sand flea Tunga penetrans is one of the agents of tungiasis, an important parasitic skin disease affecting humans and several mammalian species. Tungiasis is mainly observed in disadvantaged rural and peripheral urban communities in Latin America and sub-Saharan Africa. The dog is a major reservoir of Tunga fleas. Hematophagous adult female Tunga spp. embed and grow in their host’s epidermis and cause cutaneous inflammatory disorders. NexGard Spectra® is an orally administered endectocide for dogs, a co-formulation of the isoxazoline afoxolaner and the macrocyclic lactone milbemycin oxime. The objective of this study was to assess the efficacy of this product against canine tungiasis.

Methods

A blinded, negative-controlled field trial was conducted in a Brazilian community known to be highly endemic for tungiasis. Sixty-six dogs naturally infected with live T. penetrans were randomly allocated to a treated group (44 dogs) and an untreated control group (22 dogs). In a first phase, dogs from the treated group were treated on days 0, 30, and 60. Efficacy was evaluated on the basis of the macroscopic parasitic skin lesions (Fortaleza classification) on days 7, 14, 21, 30, 45, 60, 75, and 90. In a second phase, to evaluate natural reinfections, all dogs were treated on day 90 and evaluated every 2 weeks thereafter until at least 30% of dogs were infected with live sand fleas.

Results

During the first phase, efficacy (reduction in live sand fleas) of 92.4% was demonstrated on day 7. From day 14 until day 90, the efficacy of NexGard Spectra® was 100%. In the second phase, all dogs were free of live T. penetrans from 15 until 45 days after the day 90 treatment; 60 days post-treatment, 11% of dogs were reinfected, and 75 days post-treatment, 40% of dogs were reinfected.

Conclusions

NexGard Spectra® was demonstrated to be highly effective against canine tungiasis. In addition to an obvious beneficial effect on the health and welfare of the treated dog, the use of this product may have a one-health benefit on human cases by controlling the main reservoir of sand fleas.

Graphical Abstract

Background

Sand fleas of the genus Tunga (Siphonaptera, Tungidae, Tunginae), also called chigoe fleas and jigger fleas, are among the agents of tungiasis, a zoonotic parasitic skin disease [1]. Tungiasis is an important and severe public health concern in tropical and subtropical regions such as Latin America, the Caribbean, and sub-Saharan Africa [2]. Tungiasis is included in the list of neglected tropical diseases by the World Health Organization, in the category of scabies and parasitic skin diseases [3]. High human prevalence can occur in disadvantaged rural and peripheral urban communities [4,5,6,7,8].

The 1-mm-long adult Tunga spp. inhabit the floor and infest a mammalian host mainly by direct skin contact. They usually affect feet in humans, pads in animals, or other skin areas with frequent floor contact. After minutes to hours of host contact, the hematophagous sand flea takes a blood meal, and within a few hours, the female penetrates the epidermis, remains embedded, and matures into a globular pea-size neosome growing by abdominal hypertrophy. The neosomy period lasts approximately 5–6 weeks before parasite death, and causes a highly morbid and intense inflammatory reaction, increasing in intensity at the late stage [9,10,11,12,13]. When the epidermis penetration phase is complete, during stage III of the Fortaleza classification, (Table 1), hundreds of eggs are released into the environment by each female. Similar to other Siphonaptera, the eggs hatch into larvae that feed on organic debris and molt into pupae that contribute to efficient and lasting environmental contamination in sand, soil, dust, dirt, and cracks in floors [5].

Table 1 Description of the Fortaleza classification
Full size table

Fourteen Tunga species are known, including the zoonotic species Tunga penetrans, the most prevalent and widely described sand flea species in Brazil [14]. Tunga penetrans frequently infests humans and domestic, semi-domestic, and wildlife mammals such as dogs, cats, pigs, cattle, goats, and rats [14,15,16,17,18]. Endemicity in humans is exacerbated in poor communities where the standards of medical care and hygiene are low, and where there is close proximity to domestic, semi-domestic, or stray reservoir animals. [1, 2, 19]. Dogs in Brazil and pigs in Africa are considered the main reservoirs [19, 20]. A prevalence of infection as high as 86% has been described in Brazilian dogs in highly endemic areas [21, 22]. The incidence of human tungiasis can reach 50% in highly endemic Brazilian areas [23]. Besides environmental infection by the off-host developing stage, inter-host transmission of adult Tunga is hypothesized to occur in crowded areas [5]. Some cases have been detected in travelers returning from endemic areas [23,24,25,26] but are probably underdiagnosed, as they generally do not require specialist treatment [23].

The control strategies for tungiasis are challenging and require an integrated approach [11, 17, 27]. They include environmental sanitation and control of immature off-host stages, human treatment, and animal reservoir management including treatment and animal–human proximity control [28]. The epidemiology is also influenced by the climate, as infection peaks are observed in dry seasons [27]. In areas with consistent climatic conditions, control is further complicated as contamination is constant throughout the year.

Afoxolaner is a systemic insecticide and acaricide compound belonging to the isoxazoline group, acting on gamma-aminobutyric acid (GABA)-gated chloride ion channels, and resulting in electrophysiological disruption of the central nervous system and death of the arthropod [29, 30]. Afoxolaner is available in three oral formulations for dogs (NexGard®, NexGard Spectra®, and NexGard® Plus, Boehringer Ingelheim Animal Health) and is indicated for the treatment and control of flea (Ctenocephalides felis and C. canis), tick, and mite infestations [31,32,33]. Afoxolaner was also demonstrated efficacious against the screwworm Cochliomyia hominivorax in dogs in a field trial in Brazil [34]. After oral administration, alone or in combination with milbemycin oxime (MO), afoxolaner is rapidly absorbed, with plasma peak levels (Tmax) observed between 2 and 6 h, and is slowly eliminated, with an elimination half-life of 15 ± 8 days, resulting in rapid onset of efficacy, and sustained efficacy lasting 1 month [35, 36]. Afoxolaner is highly bound to plasma proteins, therefore acting through a systemic pathway on hematophagous arthropods.

The efficacy of fluralaner, another isoxazoline compound, has already been demonstrated against T. penetrans in dogs [37].

This manuscript describes a field study designed to assess the afoxolaner insecticidal activity against T. penetrans in naturally infected dogs in an endemic region of Brazil.

Methods

This study was a blinded, negative-controlled, and randomized field trial conducted in one site in Brazil from November 2021 to May 2022. It was conducted in accordance with the principles of good clinical practice (VICH GL 9).

The study site was located in a resource-poor rural community (district of Aritaguá, Ilhéus, Bahia) known to have a high tungiasis prevalence [21, 22]. The local climate is steady throughout the year, with average annual temperatures ranging from 22 to 25 °C, and with a regular and consistent rainfall regime. The village was populated with 368 residents and approximately 100 dogs. Some residences were unfinished and did not have concrete floors, and streets were unpaved and consisted of sand and earth. Dogs were semi-domestic and roamed freely, but were habituated to return to and dwell in the same house or house yard.

The investigated veterinary product (IVP) was NexGard Spectra®, a palatable tablet formulation for oral administration to dogs, containing afoxolaner and MO. It is recognized that MO, the nematicide compound of the product, has a negligible effect on arthropods when given orally once a month [38, 39], and thus this compound does not bring any additional ectoparasiticide efficacy to that of afoxolaner. Depending on body weight, the administered doses range from 2.5 to 5.3 mg/kg afoxolaner and 0.5–1.1 mg/kg MO. NexGard Spectra® is indicated for the treatment of Ctenocephalides felis and C. canis fleas, ticks, mites, gastrointestinal nematodes, lungworms, and eyeworms, and for the prevention of heartworm disease [39]. During the study, all treatments were administered according to label instructions.

The canine tungiasis was primarily evaluated using the Fortaleza classification [12] (Table 1) and secondarily using the severity score for acute dog tungiasis (SCADT) classification (Table 2).

Table 2 Description of severity score for acute dog tungiasis (SCADT)
Full size table

At each visit, a detailed skin inspection was performed, including paws, limbs, tail, mammary glands, abdomen, testes, and nose, to search for tungiasis lesions. Before examination, the dogs’ paws were cleaned using a brush and water to improve lesion detection and scoring. Identified lesions were staged according to the Fortaleza classification. Each clinical sign was scored for each affected area, and the results added to obtain the SCADT. If an animal exceeded a SCADT score of 22, the dog was treated by surgical removal of Tunga spp. neosomes and removed from the study for ethical reasons, as it was not needed in this trial.

To be eligible for inclusion, dogs had to be affected with at least three tungiasis lesions of stage II or III of the Fortaleza classification [12], corresponding to the live embedded stages of the fleas.

The dogs had to comply with the NexGard Spectra® label (e.g., weighing at least 2 kg, aged at least 8 weeks), and had to be healthy and have a suitable temperament. Ten neosomes were collected from 10 different dogs for morphological speciation and underwent identification in the Veterinary Parasitology Laboratory of Santa Cruz State University, Brazil. All neosomes were identified as being from T. penetrans.

Throughout the study, usual husbandry conditions were maintained, and commercial canine feed was provided by study personnel. At the beginning of the study, dogs from the untreated control group were administered a dewormer containing febantel, pyrantel pamoate, and praziquantel (Therax Plus, UCB Pharma) by unblinded personnel. All study activities were performed at the respective dogs’ house yard and with documented owner consent. Personnel responsible for the parasitic and clinical evaluations were blinded to study group.

Dogs were assigned to the treated group or the untreated control group on a 2:1 ratio, per order of inclusion and on the basis of a random allocation list. Each individual dog was an experimental unit, and dogs from the same house yard were assigned independently to the treated or the untreated control group.

Forty-four dogs were assigned to the IVP-treated group, and 41 completed the study. Twenty-two dogs were assigned to the untreated control group, and 21 completed the study. The four dogs that did not complete the study were removed for reasons unrelated to the study design or the IVP; all interim data obtained from these dogs were nevertheless included in the analyses. All dogs were mongrels and were identified with a subcutaneous microchip. The main characteristics of the dogs and baseline measurements are described in Table 3.

Table 3 Distribution of age, weight, sex, flea count, and total severity score (SCADT) on day 0
Full size table

The study was conducted in two phases. Phase I was designed to evaluate the efficacy of three monthly treatments with the IVP against tungiasis. Phase II was designed to evaluate natural reinfections after a single treatment in this specific environment.

In phase I, dogs from the treated group were administered IVP orally on days 0, 30, and 60. The control group remained untreated. Dogs were evaluated for tungiasis weekly after the first treatment and then every 2 weeks after the second and third treatments, i.e., on days (±2) 7, 14, 21, 30, 44, 60, 74, 90. In phase II, on day 90, all dogs (including the previously treated group and the control group) received an IVP treatment and were evaluated every 2 weeks until reappearance of tungiasis lesions in at least 30% of dogs. Dogs were thus evaluated on days (±2) 105, 120, 135, 150, and 165, when the 30% reinfection threshold had been exceeded.

Data analysis

Primary variable The T. penetrans lesion counts of stages II and III of the Fortaleza classification were the primary variable, as they corresponded to the live embedded stages.

Efficacy was calculated on each evaluation time point using two methods:

  • Group comparison, where the groups were compared at each post-treatment evaluation time point, using the formula:

    $${\text{Efficacy}}\,(\%)\, = \,\left( {M*{\text{ control}}--M* {\text{treated}}} \right)/\left( {M*{\text{ control}}} \right) \times {1}00$$

    *M = GM (geometric mean) of combined stage II and III lesions (Fortaleza classification). For information, efficacy based on AM (arithmetic means) was also calculated.

The data obtained were analyzed per the Shapiro–Wilk method to determine whether the distribution was parametric or non-parametric. Considering that the distribution was non-parametric, the Wilcoxon test was used.

  • Comparison of the percentage of dogs infected with parasites in each group, at each post-treatment evaluation time point, using the formula:

    $${\text{Efficacy}}\,\left( \% \right) = [\left( {{\text{Nc}}--{\text{Nt}}} \right)/\left( {{\text{Nc}}} \right] \times {1}00$$

    Nc = % of animals with active lesions in the control group, Nt = % of animals with active lesion in the treated group.

The 95% confidence limits for the percentage of dogs free of live T. penetrans were calculated as Wilson scoring intervals.

Total severity scores (SCADT) were the secondary variable and were calculated by study day and study group.

The evaluation of the SCADT for canine tungiasis was based on the mean and median of the clinical signs on each assessment day. Mean SCADT scores in the two groups were compared at each post-treatment evaluation time point using the Wilcoxon test (exact), with the level of significance set to α = 0.05 (two-sided).

Results

Phase I: evaluation of the efficacy of three monthly treatments with NexGard Spectra®

Primary variable

The efficacy results based on Fortaleza classification II or III lesions in the treated group compared to the untreated control group are detailed in Table 4.

Table 4 Phase I, efficacy results based on means of Fortaleza classification II or III lesions in the IVP-treated group and the untreated control group
Full size table

On day 0 before treatment, the average baseline sum of both levels of lesion was 18.8 in the treated group (n = 44) and 19.4 in the untreated control group (n = 22). On day 7 the efficacy (percent reduction) observed in the treated group in comparison to the untreated control group was 92.4% by geometric mean (94.5% by arithmetic mean). From day 14 until day 90, the efficacy of the IVP was maintained at 100%.

The percentage of flea-free dogs in the treated group and the untreated control group are shown in Table 5.

Table 5 Phase I, efficacy results based on the percentage of Tunga-free dogs in the IVP-treated group and the untreated control group
Full size table

As per inclusion requirements, on day 0, 100% of dogs from each group were infected by live embedded T. penetrans (i.e., were affected with Fortaleza level II and/or III lesions). On day 7, 25/44 dogs (57%) in the treated group and 2/22 (9%) dogs in the untreated control group were free of live T. penetrans. From day 14 to day 90, all dogs in the treated group were free of live T. penetrans. The infection levels improved somewhat in the untreated control dogs, especially during the third month (on days 60, 75, and 90), when 52–62% of dogs were diagnosed free of live T. penetrans, nevertheless remaining significantly lower than the treated group over the 3 months (P < 0.0001 at all time points).

Total severity scores (SCADT)

The SCADT by study day and study group and the corresponding intervals are shown in Table 6. Mean total SCADT differed significantly between groups on days 7, 14, 21, 30, 60, and 90, whilst on days 44 and 74 there was no significant difference between groups.

Table 6 Phase I, SCADT severity scores per group and time point
Full size table

Phase II: evaluation of natural reinfections after a single treatment

On day 90, all dogs were treated and then monitored every 2 weeks for the reappearance of active tungiasis lesions (i.e., level II or III Fortaleza classification).

The percentages of dogs that remained free of active tungiasis lesions are shown in Table 7.

Table 7 Phase II, percentage of Tunga-free dogs after a single administration of NexGard Spectra® on day 90
Full size table

The 10 dogs that previously belonged to the untreated control group and were affected with active tungiasis lesions before the day 90 treatment confirmed the results of phase I, as they were cleared from active lesions on day 105, 15 days after their IVP treatment. All 62 dogs remained free of new infection with T. penetrans until 45 days after treatment (day 135). Eleven percent of dogs were reinfected 60 days after treatment (day 150) and 40% were reinfected 75 days after treatment (day 165) when the study was closed.

In both phases, no adverse reaction related to treatment was observed in any dog.

Discussion

The results of this field trial demonstrated a high level of efficacy of the IVP for the treatment and control of tungiasis in dogs, in a highly endemic area. Phase I demonstrated that 100% efficacy was achieved within 2 weeks after the first treatment and was maintained at 100% with monthly treatments. Phase II demonstrated that a single treatment provided sustained efficacy of 100% for at least 45 days, but that a regular treatment regimen was necessary, as environmental reinfection occurred afterwards. This study also demonstrated that the treatment significantly improved the dermal skin lesions associated with T. penetrans infections.

During phase I, even though significantly lower than in the IVP group, the number of Tunga-free dogs increased somewhat in the untreated control dogs, in particular 6 weeks (from day 44) after the first afoxolaner administration to the IVP-treated group (Table 4). The three monthly treatments with afoxolaner in an important proportion of the local dog population may have impacted the parasite turnover of the environmental contamination, thus reducing the infection rate in the untreated dogs. Figures 1 and 2 illustrate the evolution of T. penetrans lesions in a treated and an untreated control dog living in the same house yard. Figures 3 and 4 illustrate the evolution of T. penetrans lesions in a treated dog and an untreated control dog living alone in different house yards, respectively.

Fig. 1
figure 1

Evolution of T. penetrans lesions for dog 19 of the treated group (source: personal collection). A, B Day 0, the dog had multiple stage II and III lesions located on the front feet pads; C day 7, the pads had no longer stages II and III lesions, stage IV lesions were visible (arrows); D day 14, E day 21, stage IV lesions were visible (arrows); F day 30; G day 60, the pads had no longer any T. penetrans lesions; H, I day 90, the pads were free of T. penetrans lesions and completely re-epithelialized

Full size image
Fig. 2
figure 2

Evolution of T. penetrans lesions for dog 18 of the untreated control group (co-living with dog 19, Fig. 3) (source: personal collection). A–C Day 0; D day 7; E day 14, F day 21; G day 30; H day 60, the dog had multiple stage II and III lesions located on the front feet pads (arrows); I day 90, the pads were free of stage II and III lesions

Full size image
Fig. 3
figure 3

Evolution of T. penetrans lesions for dog 23 of the treated group (source: personal collection). A Day 0, the dog had multiple stage II and III lesions located on the front feet pads; B, C day 7, stage II and III lesions were no longer visible, stage V lesions were visible (arrows in B); D day 14 and E, F day 21, the pads remained free of stages II and III lesions; G day 30, H day 60, and I day 90, the pads were free of T. penetrans lesions and were completely re-epithelialized

Full size image
Fig. 4
figure 4

Evolution of T. penetrans lesions for dog 36 of the untreated control group (not co-living with a treated dog) (source: personal collection). A Day 0, B day 7, the dog had multiple stage II and III lesions located on the front feet pads; C day 14, in addition to the stage II and III lesions, stage IV lesions can be observed (arrows); D, E day 21, F day 30, G day 60, and H day 90, the pads remained infected with T. penetrans lesions

Full size image

The most common treatment of tungiasis in humans is the mechanical extraction of the parasitic lesion followed by local/systemic symptomatic treatment of the inflammatory and secondary infectious consequences. Nevertheless, a sustained and efficient reduction in the local Tunga spp. prevalence in impoverished endemic areas requires drastic environmental control measures, which may be inefficient, difficult, and unaffordable in such areas [2, 11]. Besides the environmental hygienic measures and human–animal proximity controls, the direct treatment of T. penetrans in dogs, a major reservoir of Tunga spp., may contribute to the control of the disease in humans and provide an efficient one-health strategy to public health authorities [17, 28].

In the present study, afoxolaner provided a convenient and efficacious solution for the control of T. penetrans in dogs. NexGard Spectra® is a highly palatable [40] chewable tablet and therefore easily accepted by dogs, which simplifies oral administration, including to individuals with a lower level of domestication that typically populate T. penetrans endemic areas. Besides palatability, which facilitates a high level of compliance, the monthly regimen of this product allows the treatment duration to be adapted in areas with seasonal peaks of infection.

NexGard Spectra® combines afoxolaner and MO. The effect of MO on arthropods is believed to be negligible [39]; however, no specific data were obtained on its effect on embedded T. penetrans. It is possible that MO also contributed to the efficacy observed in this study, but only for a short duration after each IVP oral administration, because of its short half-life, i.e., 1.6 days ± 0.4 days for the A3 form and 3.3 days ± 1.4 days the A4 form [36, 38], and therefore in a negligible way relative to afoxolaner. Nevertheless, the decision was made to avoid the use of MO in the control group in this study, to avoid any confusion about the observations made on the untreated control animals. The dewormer used in the control group, a combination of pyrantel pamoate, fenbendazole, and praziquantel, did not have any active ingredient with a potential effect on T. penetrans.

Even though dogs are the main reservoir species of T. penetrans, the environment may be loaded with infective stages for a significant period, and other animal species may also play a reservoir role. Therefore, it would be highly valuable to further assess the correlation between human cases and the control of the parasite in dogs in endemic communities.

If confirmed, the obvious one-health benefit of the use of NexGard Spectra® in dogs in relation to tungiasis may be further sustained by its nematicidal spectrum that includes several other zoonotic agents for which dogs are a reservoir [41]. For example, Ancylostoma spp. and Toxocara spp. are, through their larva migrans effect, another significant public health concern in many regions of the world including areas of tungiasis [42,43,44,45], and NexGard Spectra® has registered efficacy against them [38].

Conclusions

This study demonstrated that monthly oral administration of afoxolaner was highly effective for the treatment and control of tungiasis in dogs, the main reservoir of T. penetrans in many endemic areas in the world. Apart from an obvious beneficial effect on the health and welfare of the treated dog, the use of this product may also have a one-health benefit.

Availability of data and materials

All available data are within the manuscript.

References

  1. Heukelbach J. Tungiasis. Rev Inst Med Trop Sao Paulo. 2005;47:307–13. https://doi.org/10.1590/s0036-46652005000600001.

    Article  PubMed  Google Scholar 

  2. Feldmeier H, Heukelbach J, Ugbomoiko US, Sentongo E, Mbabazi P, von Samson-Himmelstjerna G, et al. Tungiasis—a neglected disease with many challenges for global public health. PLoS Negl Trop Dis. 2014;8:e3133. https://doi.org/10.1371/journal.pntd.0003133.

    Article  PubMed  PubMed Central  Google Scholar 

  3. World Health Organization (WHO). Tungiasis. https://www.who.int/news-room/fact-sheets/detail/tungiasis.

  4. Joseph JK, Bazile J, Mutter J, Shin S, Ruddle A, Ivers L, et al. Tungiasis in rural Haiti: a community-based response. Trans R Soc Trop Med Hyg. 2006;100:970–4. https://doi.org/10.1016/j.trstmh.2005.11.006.

    Article  PubMed  Google Scholar 

  5. Elson L, Thielecke M, Fillinger U, Feldmeier H. Infection with tungiasis through interhost movement of adult female sand fleas, Tunga penetrans. Trans R Soc Trop Med Hyg. 2022;116:85–6. https://doi.org/10.1093/trstmh/trab117.

    Article  PubMed  Google Scholar 

  6. Mwangi JN, Ozwara HS, Gicheru MM. Epidemiology of Tunga penetrans infestation in selected areas in Kiharu constituency, Murang’a County, Kenya. Trop Dis Travel Med Vaccines. 2015;1:13. https://doi.org/10.1186/s40794-015-0015-4.

    Article  PubMed  PubMed Central  Google Scholar 

  7. Tamene A. Prevalence and associated factors of Tunga penetrans infestation among 5–14-year-olds in rural Ethiopia. PLoS ONE. 2021;16:e0259411. https://doi.org/10.1371/journal.pone.0259411.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Mutebi F, McNeilly H, Thielecke M, Reichert F, Wiese S, Mukone G, et al. Prevalence and infection intensity of human and animal tungiasis in Napak District, Karamoja, Northeastern Uganda. Trop Med Infect Dis. 2023;8:111. https://doi.org/10.3390/tropicalmed8020111.

    Article  PubMed  PubMed Central  Google Scholar 

  9. Harvey TV, Dos Santos FZ, Dos Santos KC, de Jesus AV, Guedes PEB, da Paixão SA, et al. Clinical and macroscopic morphological features of canine tungiasis. Parasitol Res. 2021;120:807–18. https://doi.org/10.1007/s00436-020-07013-7.

    Article  PubMed  Google Scholar 

  10. Feldmeier H, Witt L, Schwalfenberg S, Linardi PM, Ribeiro RA, Capaz RA, et al. Investigations on the biology, epidemiology, pathology and control of Tunga penetrans in Brazil. VI. Natural history of the infestation in laboratory-raised Wistar rats. Parasitol Res. 2007;102:1–13. https://doi.org/10.1007/s00436-007-0731-4.

    Article  PubMed  Google Scholar 

  11. Heukelbach J. Revision on tungiasis: treatment options and prevention. Expert Rev Anti Infect Ther. 2006;4:151–7. https://doi.org/10.1586/14787210.4.1.151.

    Article  CAS  PubMed  Google Scholar 

  12. Eisele M, Heukelbach J, Van Marck E, Mehlhorn H, Meckes O, Franck S, et al. Investigations on the biology, epidemiology, pathology and control of Tunga penetrans in Brazil: I. Natural history of tungiasis in man. Parasitol Res. 2003;90:87–99. https://doi.org/10.1007/s00436-002-0817-y.

    Article  PubMed  Google Scholar 

  13. Mutebi F, Krücken J, Feldmeier H, Waiswa C, Mencke N, von Samson-Himmelstjerna G. Tungiasis-associated morbidity in pigs and dogs in endemic villages of Uganda. Parasit Vectors. 2016;9:44. https://doi.org/10.1186/s13071-016-1320-0.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Harvey TV, Linardi PM, Carlos RSA, Heukelbach J. Tungiasis in domestic, wild, and synanthropic animals in Brazil. Acta Trop. 2021;222:106068. https://doi.org/10.1016/j.actatropica.2021.106068.

    Article  PubMed  Google Scholar 

  15. De Avelar DM, Facury Filho EJ, Linardi PM. A new species of Tunga (Siphonaptera: Tungidae) parasitizing cattle from Brazil. J Med Entomol. 2013;50:679–84. https://doi.org/10.1603/me12221.

    Article  PubMed  Google Scholar 

  16. Heukelbach J, Costa AM, Wilcke T, Mencke N, Feldmeier H. The animal reservoir of Tunga penetrans in severely affected communities of north-east Brazil. Med Vet Entomol. 2004;18:329–35. https://doi.org/10.1111/j.0269-283X.2004.00532.x.

    Article  CAS  PubMed  Google Scholar 

  17. Mutebi F, Krücken J, Feldmeier H, Waiswa C, Mencke N, Sentongo E, et al. Animal reservoirs of zoonotic tungiasis in endemic rural villages of Uganda. PLoS Negl Trop Dis. 2015;9:e0004126. https://doi.org/10.1371/journal.pntd.0004126.

    Article  PubMed  PubMed Central  Google Scholar 

  18. Widmer CE, Azevedo FC. Tungiasis in a free-ranging jaguar (Panthera onca) population in Brazil. Parasitol Res. 2012;110:1311–4. https://doi.org/10.1007/s00436-011-2625-8.

    Article  PubMed  Google Scholar 

  19. Heukelbach J, de Oliveira FA, Hesse G, Feldmeier H. Tungiasis: a neglected health problem of poor communities. Trop Med Int Health. 2001;6:267–72. https://doi.org/10.1046/j.1365-3156.2001.00716.x.

    Article  CAS  PubMed  Google Scholar 

  20. Mutebi F, Krücken J, von Samson-Himmelstjerna G, Waiswa C, Mencke N, Eneku W, et al. Animal and human tungiasis-related knowledge and treatment practices among animal keeping households in Bugiri District, South-Eastern Uganda. Acta Trop. 2018;177:81–8. https://doi.org/10.1016/j.actatropica.2017.10.003.

    Article  PubMed  Google Scholar 

  21. Harvey TV, Heukelbach J, Assunção MS, Fernandes TM, da Rocha CMBM, Carlos RSA. Seasonal variation and persistence of tungiasis infestation in dogs in an endemic community, Bahia State (Brazil): longitudinal study. Parasitol Res. 2019;118:1711–8. https://doi.org/10.1007/s00436-019-06314-w.

    Article  PubMed  Google Scholar 

  22. Harvey TV, Heukelbach J, Assunção MS, Fernandes TM, da Rocha CM, Carlos RS. Canine tungiasis: high prevalence in a tourist region in Bahia state, Brazil. Prev Vet Med. 2017;139:76–81. https://doi.org/10.1016/j.prevetmed.2017.02.009.

    Article  PubMed  Google Scholar 

  23. Lefebvre M, Capito C, Durant C, Hervier B, Grossi O. Tungiasis: a poorly documented tropical dermatosis. Med Mal Infect. 2011;41:465–8. https://doi.org/10.1016/j.medmal.2011.05.007.

    Article  CAS  PubMed  Google Scholar 

  24. Dialynas M, Karakosta P, Haniotis V, Fanouriakis A, Panagiotaki E, Maraki S. Imported human tungiasis in Greece. Travel Med Infect Dis. 2009;7:375–7. https://doi.org/10.1016/j.tmaid.2009.09.006.

    Article  PubMed  Google Scholar 

  25. Mukai Y. Tunga penetrans in a Sub-Saharan African desert traveler. Intern Med. 2020;59:2441. https://doi.org/10.2169/internalmedicine.4652-20.

    Article  PubMed  PubMed Central  Google Scholar 

  26. Sachse MM, Guldbakke KK, Khachemoune A. Tunga penetrans: a stowaway from around the world. J Eur Acad Dermatol Venereol. 2007;21:11–6. https://doi.org/10.1111/j.1468-3083.2006.01888.x.

    Article  CAS  PubMed  Google Scholar 

  27. Heukelbach J, Wilcke T, Harms G, Feldmeier H. Seasonal variation of tungiasis in an endemic community. Am J Trop Med Hyg. 2005;72:145–9.

    Article  PubMed  Google Scholar 

  28. Dos Santos KC, Brandão Guedes PE, Teixeira JBC, Harvey TV, Carlos RSA. Treatment of animal tungiasis: what’s new? Trop Med Infect Dis. 2023;8:142. https://doi.org/10.3390/tropicalmed8030142.

    Article  PubMed  PubMed Central  Google Scholar 

  29. Ozoe Y, Asahi M, Ozoe F, Nakahira K, Mita T. The antiparasitic isoxazoline A1443 is a potent blocker of insect ligand-gated chloride channels. Biochem Biophys Res Commun. 2010;391:744–9. https://doi.org/10.1016/j.bbrc.2009.11.131.

    Article  CAS  PubMed  Google Scholar 

  30. Shoop WL, Hartline EJ, Gould BR, Waddel ME, McDowell RG, Kinney JB, et al. Discovery and mode of action of afoxolaner, a new isoxazoline parasiticide for dogs. Vet Parasitol. 2014;201:79–189.

    Article  Google Scholar 

  31. Lebon W, Beccati M, Bourdeau P, Brement T, Bruet V, Cekiera A, et al. Efficacy of two formulations of afoxolaner (NexGard® and NexGard Spectra®) for the treatment of generalised demodicosis in dogs, in veterinary dermatology referral centers in Europe. Parasit Vectors. 2018;11:506. https://doi.org/10.1186/s13071-018-3083-2.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Otranto D. NEXGARD®. Afoxolaner, a new oral insecticide-acaricide to control fleas and ticks in dogs. Editorial. Vet Parasitol. 2014;201:177–8. https://doi.org/10.1016/j.vetpar.2014.02.029.

    Article  PubMed  Google Scholar 

  33. Beugnet F, de Vos C, Liebenberg J, Halos L, Larsen D, Fourie J. Efficacy of afoxolaner in a clinical field study in dogs naturally infested with Sarcoptes scabiei. Parasite. 2016;23:26. https://doi.org/10.1051/parasite/2016026.

    Article  PubMed  PubMed Central  Google Scholar 

  34. Cutolo AA, Perier N, Menz I, Thyssen P, Silva FO, Beugnet F. Efficacy of afoxolaner (NexGard®) on the treatment of myiasis caused by the New World screwworm fly Cochliomyia hominivorax (Diptera: Calliphoridae) in naturally infested dogs. Vet Parasitol Reg Stud Rep. 2021;24:100569. https://doi.org/10.1016/j.vprsr.2021.100569.

    Article  Google Scholar 

  35. Letendre L, Harriman J, Drag M, Mullins A, Malinski T, Rhebein S. The intravenous and oral pharmacokinetics of afoxolaner and milbemycin oxime when used as a combination chewable parasiticide for dogs. J Vet Pharmacol Therap. 2016. https://doi.org/10.1111/jvp.12332.

    Article  Google Scholar 

  36. Letendre L, Harriman J, Huang R, Kvaternick V, Drag M, Larsen DL. The intravenous and oral pharmacokinetics of afoxolaner, a novel isoxazoline, used as a monthly chewable antiparasitic for dogs. Vet Parasitol. 2014;201:190–7.

    Article  CAS  PubMed  Google Scholar 

  37. Dos Santos KC, Chiummo RM, Heckeroth AR, Zschiesche E, Brandão Guedes PE, Harvey TV, et al. Efficacy of oral fluralaner (Bravecto) against Tunga penetrans in dogs: a negative control, randomized field study in an endemic community in Brazil. PLoS Negl Trop Dis. 2022;16:e0010251. https://doi.org/10.1371/journal.pntd.0010251.

    Article  PubMed  PubMed Central  Google Scholar 

  38. EMA. CVMP Assessment Report for NexGard Spectra (EMEA/V/C/003842/0000) (2014).

  39. EMA. CVMP assessment report for type II variation for NexGard Spectra (EMEA/V/C/003842/II/0019) (2019).

  40. Perier N, Carithers DS, Everett WR, Wongnak P, Chalvet-Monfray K, Beugnet F. Preference in dogs of two oral endectoparasiticide formulations: NexGard Spectra® (afoxolaner and milbemycin oxime) and Credelio® Plus (lotilaner and milbemycin oxime). Open J Vet Med. 2021;11:289–98.

    Article  CAS  Google Scholar 

  41. Rehbein S, Dorr P, Bowman DD, Crafford D, Kusi I, Postoli R, et al. Efficacy of afoxolaner plus milbemycin oxime chewable tablets against naturally acquired intestinal nematodes in dogs. Vet Parasitol. 2016;217:29–35. https://doi.org/10.1016/j.vetpar.2015.12.032.

    Article  CAS  PubMed  Google Scholar 

  42. Bowman DD. History of Toxocara and the associated larva migrans. Adv Parasitol. 2020;109:17–38. https://doi.org/10.1016/bs.apar.2020.01.037.

    Article  PubMed  Google Scholar 

  43. Heukelbach J, Mencke N, Feldmeier H. Editorial: Cutaneous larva migrans and tungiasis: the challenge to control zoonotic ectoparasitoses associated with poverty. Trop Med Int Health. 2002;7:907–10. https://doi.org/10.1046/j.1365-3156.2002.00961.x.

    Article  PubMed  Google Scholar 

  44. Marques JP, Guimarães Cde R, Boas AV, Carnaúba PU, de Moraes J. Contamination of public parks and squares from Guarulhos (São Paulo State, Brazil) by Toxocara spp. and Ancylostoma spp. Rev Inst Med Trop Sao Paulo. 2012;54:267–71. https://doi.org/10.1590/s0036-46652012000500006.

    Article  PubMed  Google Scholar 

  45. Silva GSD, Ferreira FC, Romera DM, Soares VE, Bonuti MR. Larva migrans in Votuporanga, São Paulo, Brazil: where does the danger hide? Rev Bras Parasitol Vet. 2020;29:e004920. https://doi.org/10.1590/S1984-29612020075.

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

We thank Boehringer Ingelheim Animal Health for funding this research project. We thank the Bahia State Research Support Foundation (FAPESB), the Brazilian National Council for Scientific and Technological Development (CNPq), and the State University of Santa Cruz (UESC) for granting scholarships. This study was financed in part by the Coordination for the Improvement of Higher Education Personnel—Brazil (CAPES)—Finance Code 001. RSAC, GRA, and FAB are class 2 research fellows from the Brazilian National Council for Scientific and Technological Development (CNPq).

Funding

The study was sponsored by Boehringer Ingelheim Animal Health.

Author information

Authors and Affiliations

  1. Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC), Campus Soane Nazaré de Andrade, Rod. Jorge Amado, Km 16 - Salobrinho, Ilhéus, Bahia, 45662-900, Brazil

    Katharine Costa dos Santos, Paula Elisa Brandão Guedes, Jamille Bispo de Carvalho Teixeira, Rebeca Costa Vitor, Anaiá da Paixão Sevá, Adan William de Melo Navarro, Ana Carolina Ribeiro Lima, Thammy Vieira Bittar, George Rêgo Albuquerque & Renata Santiago Alberto Carlos

  2. Boehringer Ingelheim Animal Health, 29 Avenue Tony Garnier, 69007, Lyon, France

    Eric Tielemans & Frederic Beugnet

  3. Missouri Research Center, Boehringer Ingelheim Animal Health, 6498 Jade Rd., Fulton, MO, 65251, USA

    Andre Antonio Cutolo

  4. College Station, USA

    Tatiani Vitor Harvey

  5. Boehringer-Ingelheim Saúde Animal, 14171 Pça. das Nações Unidas, 18° andar (Torre B), São Paulo, SP, 01449-010, Brazil

    Karin Denise Botteon

  6. Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul (UFMS), Av. Sen. Filinto Müler, 2443 - Pioneiros, Campo Grande, Mato Grosso do Sul, 79070-900, Brazil

    Fernando de Almeida Borges

Authors
  1. Katharine Costa dos Santos
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Eric Tielemans
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Andre Antonio Cutolo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Paula Elisa Brandão Guedes
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tatiani Vitor Harvey
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jamille Bispo de Carvalho Teixeira
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Rebeca Costa Vitor
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Anaiá da Paixão Sevá
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Adan William de Melo Navarro
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Ana Carolina Ribeiro Lima
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Karin Denise Botteon
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Thammy Vieira Bittar
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. George Rêgo Albuquerque
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. Fernando de Almeida Borges
    View author publications

    You can also search for this author in PubMed Google Scholar

  15. Frederic Beugnet
    View author publications

    You can also search for this author in PubMed Google Scholar

  16. Renata Santiago Alberto Carlos
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Project administration: FB, RSAC, ET, AAC, KDB. Investigation: KCS, PEBG, TVH, JBCT, RCV, AWML, ACRL, TVB, GRA, APS, RSAC. Writing—original draft preparation: ET. Writing—review and editing: FB, RSAC. Supervision: RSAC, KDB, AAC. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Eric Tielemans or Renata Santiago Alberto Carlos.

Ethics declarations

Ethics approval and consent to participate

All animal procedures in these studies were reviewed and approved by the Ethics Committee for Animal Experimentation (CEUA) of the State University of Santa Cruz (UESC), Ilhéus, Bahia, Brazil, under protocol number 017/2021, and were in compliance with all applicable sections of the Final Rules of the Animal Welfare Act regulations (9 CFR), the current AVMA Guidelines, and ETS 123 (European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes). Informed consent was obtained from owners of all animals included in the study.

Consent for publication

Not applicable.

Competing interests

As they are employees of Boehringer Ingelheim Animal Health, the following authors have a conflict of interest: Frederic Beugnet, Eric Tielemans, Andre Antonio Cutolo, and Karin Denise Botteon. The other authors declare no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

dos Santos, K.C., Tielemans, E., Cutolo, A.A. et al. Efficacy of an oral formulation of afoxolaner and milbemycin oxime against Tunga penetrans in naturally infected dogs. Parasites Vectors 16, 446 (2023). https://doi.org/10.1186/s13071-023-06063-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s13071-023-06063-x

Keywords

Parasites & Vectors

ISSN: 1756-3305

Contact us