Fig. 2

rab27 silencing shifts the size of EVs being released by tick cells. A Experimental schematic for vesicle collection from tick cells. Tick ISE6 cells were nucleofected with small interfering (si-rab27) (green) or scrambled (sc-rab27) (gray) RNA for tick rab27. After 72 h, the cell culture media was replaced with vesicle free (VF) media. 24-h later, vesicles were collected through a series of tabletop and ultracentrifuge steps and measured using a nanosight machine. Graphs are representative of one two independent experiments. B Total vesicle amount released by tick cells does not change between the si-rab27 or sc-rab27 treatments. Statistical significance was evaluated by an unpaired, two-tailed t-test. Mean ± standard deviation (SD) is plotted. ns = not significant, P > 0.05. C Distribution of vesicles collected from sc-rab27 treated cells and compared to the si-rab27 treatment ranging from 0 to 600 nm