Novel trypanosomatid species detected in Mongolian pikas (Ochotona pallasi) and their fleas in northwestern China

Abstract

Background

In the family Trypanosomatidae, the genus Trypanosoma contains protozoan parasites that infect a diverse range of hosts, including humans, domestic animals, and wildlife. Wild rodents, as natural reservoir hosts of various pathogens, play an important role in the evolution and emergence of Trypanosomatidae. To date, no reports are available on the trypanosomatid infection of pikas (Lagomorpha: Ochotonidae).

Methods

In this study, Mongolian pikas and their fleas were sampled at the China–Mongolia border, northwestern China. The samples were analyzed with polymerase chain reaction (PCR) and sequencing for the presence of Trypanosomatidae on the basis of both the 18S ribosomal RNA (18S rRNA) gene and the glyceraldehyde-3-phosphate dehydrogenase (gGAPDH) gene. The morphology of trypomastigotes was also observed in peripheral blood smears by microscopy.

Results

Molecular and phylogenetic analyses revealed a new genotype of the Trypanosoma lewisi clade that was found both in pika blood and flea samples. This genotype, which probably represents a new species, was provisionally designated as “Trypanosoma sp. pika”. In addition, a novel genotype belonging to the genus Blechomonas of Trypanosomatidae was detected in fleas. On the basis of its molecular and phylogenetic properties, this genotype was named Blechomonas luni-like, because it was shown to be the closest related to B. luni compared with other flea-associated trypanosomatids.

Conclusions

To the best of our knowledge, this is the first study to report any trypanosomatid species in Mongolian pikas and their fleas. Further studies are needed to investigate the epidemiology of these protozoan parasites, as well as to evaluate their pathogenicity for humans or domestic animals.

Graphical Abstract

Background

Currently, the family Trypanosomatidae (Euglenozoa: Kinetoplastida) includes 24 genera, as exemplified by Trypanosoma, Leishmania, Crithidia, Leptomonas, and Blechomonas [1]. Trypanosoma species are parasites found in a broad range of domestic and wild vertebrate hosts (such as horses, deer, elephants, camelids, equines, buffaloes) and are transmitted by blood-sucking arthropod (e.g., fly, tick, and flea) or leech vectors [2]. Human pathogenic trypanosomes include Trypanosoma brucei gambiense and T. brucei rhodesiense causing sleeping sickness in Africa and T. cruzi as the etiological agent of Chagas disease in South America [2]. These, as well as other Trypanosoma species (e.g., T. evansi, causing surra) are also important in veterinary medicine. Taxonomically, the genus Trypanosoma is divided into six groups: the T. cruzi clade, the T. brucei clade, the T. pestanai clade, the T. irwini clade, the T. theileri clade, and the T. lewisi clade [1, 3].

Pikas (Lagomorpha: Ochotonidae) are small, rabbit-like mammals with Holarctic distribution. This family of lagomorphs is composed of a single genus, Ochotona, including 30 species [4]. The majority of pika species are found in West and Central Asian countries. The Mongolian pika (Ochotona pallasi) is only indigenous to a small area at the junction of three countries: China, Mongolia, and Russia [5]. Living in the alpine meadow ecosystem, it is closely associated with a broad range of wildlife, as well as domestic animals and humans [6]. However, epidemiological research on Mongolian pikas was almost exclusively conducted in the context of Yersinia pestis, and information on the occurrence and prevalence of Trypanosomatidae species in these lagomorphs is still scarce.

The aim of the present study was to uncover Trypanosomatidae species and their potential vectors at the China–Mongolia border in northwestern China.

Methods

Sample collection and identification

Between 2021 and 2023, a total of 83 Mongolian pikas were collected at 15 sampling sites in Beitashan Mountain (coordinates: 45.37° N, 90.53° E, elevation: 1653.7 MASL) at the border region near Mongolia in northwestern China (sampling sites are shown in Additional file 1). These pikas were captured by Sherman traps, which were placed at the entrances of occupied burrows. Each survey site had 150 traps that were checked twice daily [7]. Fleas were collected from individual Mongolian pikas by brushing their fur. Blood smears were prepared from the peripheral circulation, stained with Giemsa. In addition, the heart, liver, spleen, lung, and kidneys were removed. Simultaneously, fleas were collected from the body surface of each pika. All fleas were morphologically and molecularly identified according to our previous work [8]. The fleas were later allocated into pools ranging from 2 to 6 specimens on the basis of species. In this way, a total of 20 flea pools were analyzed.

Detection, sequencing, and phylogenetic analysis

DNA extractions from the blood samples and fleas were carried out using the TIANamp Genomic DNA Kit (TIANGEN, Beijing, China). PCR targeting 850-bp-long part of 18S ribosomal RNA gene (18S rRNA) of Trypanosomatidae was also performed. The primer sequences were as follows: rrf-OF: 5ʹ-CACCCGCGGTAATTCCAGC-3ʹ, and rrf-OR: 5ʹ-CTGAGACTGTAACCTCAA-3ʹ [9]. The PCR cycling conditions for Trypanosomatidae detection consisted of an initial 5-min denaturation at 94 ℃, followed by 35 cycles at 94 ℃ for 40 s, 60 ℃ for 40 s, and 72 ℃ for 40 s, with a final extension at 72 ℃ for 10 min. To confirm positivity of PCR, it was attempted to amplify an additional genetic marker, an approximately 820-bp-long fragment of the gGAPDH gene, encoding glyceraldehyde-3-phosphate dehydrogenase of trypanosomes. The outer reaction of this nested PCR was conducted with primers gGAPDH-F1: 5ʹ- CTYMTCGGNAMKGAGATYGAYG-3ʹ and gGAPDH-R1: 5ʹ- GRTKSGARTADCCCCACTCG-3ʹ. The second round PCR was performed with inner primers gGAPDH-F and gGAPDH-R2: 5ʹ-GTTYTGCAGSGTCGCCTTGG-3ʹ [10]. The PCR conditions were the same as in the 18S rRNA PCR, except that the annealing temperature was 50 ℃.

Fleas were identified molecularly by targeting an approximately 1000-bp-long part of the 18S ribosomal RNA gene (18S rRNA). Primer sequences were as follows: 18S rRNA-F (5ʹ-CCTGAGAAACGGCTACCACATC-3ʹ) and 18S rRNA-R (5ʹ-GCATCACAGACCTGTTATTGC-3ʹ) [11]. The conditions of the flea-specific PCR were the same as in the 18S rRNA PCR of Trypanosomatidae, except that the annealing temperature was 60 ℃. The PCRs were run in a Mastercycler X50s equipment (Eppendorf, Germany).

All PCRs were performed including a sequence-verified positive control and negative control (double-distilled water). PCR products were electrophoretically separated in a 1.5% (w/v) agarose gel stained with GoldView II, purified using the TIANgel Midi Purification Kit (Tiangen, Beijing, China) and sequenced. Sequences were compared with GenBank data using the BLAST program (http://www.ncbi.nlm.nih.gov/BLAST/). New sequences were submitted to GenBank (accession number for flea 18S rRNA gene: OR701822; for trypanosome 18S rRNA gene: OR690791, OR700018, and OR701933; for trypanosome gGAPDH gene: PP199391-PP199393). Phylogenetic trees were constructed using the neighbor-joining method in MEGA 7.0 software.

Results

Peripheral blood smear analysis

In the blood smears, fusiform trypomastigotes were observed that possessed a free-flagellar end and undulating membrane. The cell body measured 15–30 μm in length and had a width of 2–5 μm. The nucleus was located in the middle of the cell body, and the kinetoplast close to the rear end (Fig. 1).

Figure 1

Light microscopy of Giemsa-stained trypomastigotes from Mongolian pika (A, B). Abbreviations: N - nucleus; K - kinetoplast; Um - undulating membrane; F - flagellum)

Flea identification

A total of 112 fleas (18 males and 94 females) were identified as Frontopsylla elatoides elatoides on the basis of their morphology and 99.6% sequence identity (990/994 bp) to the 18S rRNA gene of this flea species collected from long-tailed ground squirrel (Spermophilus undulatus) in China (KY593303).

Molecular and phylogenetic analyses

The DNA of Trypanosomatidae was successfully amplified from the blood of each Mongolian pika. The corresponding 18S rRNA sequence was closest related to a Trypanosoma sp. from Anderson’s red-backed vole (Eothenomys andersoni) from Japan (AB242276), and to T. microti from field vole (Microtus agrestis) from England (AJ009158), showing 99.5% (830/834 bp) identity. This new genotype was provisionally named as Trypanosoma sp. pika. Phylogenetically, it clustered in the clade of T. lewisi (Fig. 2), but separately from other species, with high (99%) support. The gGAPDH sequence of this new genotype was closest related to T. lewisi from Rattus omanicus sampled in Indonesia (LC369597) and to T. microti from field vole (Microtus agrestis) from England (AJ620273), showing 97.3–98.6% identity (Additional file 2).

Figure 2

Phylogenetic tree of Trypanosomatidae species from Mongolian pikas and their fleas based on the 18S rRNA gene. The new sequences provided in the present study are indicated by a black triangle (followed by the accession number)

Out of the five PCR-positive flea pools, one was confirmed to contain the DNA of Trypanosoma sp. pika, showing 100% sequence identity to the genotype detected in the blood of pikas. The trypanosomatid species in the remaining four PCR-positive flea pools was identified as Blechomonas luni-like. This genotype was closest related to B. luni from the flea Chaetopsylla globiceps in Czechia (MH055750), sharing 98.3% (953/970 bp) identity. The gGAPDH sequence of this genotype was closest related to B. luni from Chaetopsylla sp. (KF054103) and to B. englundi from Monopsyllus sciurorum (KF054101) sampled in the Czechia, showing 87.5–88.7% identity. The phylogenetic clustering of the latter received high (100%) support and was confirmed among other flea-associated Blechomonas spp. (Fig. 2).

Discussion

Most studies mainly focus on domestic and companion animals as the reservoirs of Trypanosomatide [12], but wildlife is also being increasingly considered as an important source of emerging and/or reemerging trypanosomes via vector-borne transmission [13, 14]. However, among wild-living lagomorphs, many species still remain unstudied, especially in the family Ochotonidae. In this study, to the best of our knowledge, the presence of Trypanosomatidae was demonstrated for the first time in Mongolian pikas and their fleas.

Flea-borne diseases are diverse and globally distributed. Among them, plague, bartonellosis, and typhus, which are caused by Yersinia pestis, bartonellae, Rickettsia typhi, and R. felis, are considered as especially severe human infections transmitted by fleas [14,15,16]. In the past, the T. lewisi clade was considered as a rat-specific (R. norvegicus and R. rattus) group of pathogens, transmitted by rat fleas (Xenopsylla cheopis and Nosopsyllus fasciatus) and non-pathogenic to humans [17, 18]. Recently, an increasing number of cases involving humans infected with members of the T. lewisi clade have been reported around the world, along with the emergence of a fatal infection [18,19,20,21]. In 2015, the resistance of this parasite to the lysis by normal human serum was reported [22]. In the present study, we detected a previously unknown Trypanosoma genotype from both Mongolian pikas and fleas, which shared 100% sequence identity. Our findings suggest that the flea F. elatoides elatoides parasitizing Mongolian pikas may serve as a carrier for this hemoflagellate. In addition, splenomegaly was noted in Trypanosoma-positive individuals, compared with the normal spleen of uninfected pikas (Additional file 3). These results necessitate further studies to evaluate the clinicopathological significance of Trypanosoma sp. pika.

Regarding vectors of Trypanosomatidae other than flies, Blechomonas luni was reported from the flea C. globiceps, T. binneyi from the leech, and T. rhipicephalis from the tick species Rhipicephalus microplus [23,24,25]. In general, members of the genus Blechomonas are associated with fleas as reservoirs or vectors [23]. Here a B. luni-like trypanosomatid parasite was detected in the flea species F. elatoides elatoides for the first time. On the basis of the topology of the 18S rRNA gene phylogenetic tree and high bootstrap supports, it is likely that both Trypanosoma sp. pika and B. luni-like genotype represent hitherto unknown species.

To date, most studies on pathogens in pikas (especially in plateau pikas) focused on Echinococcus shiquicus, Toxoplasma gondii, H5N1 avian influenza virus, and H9N2 avian influenza virus [26,27,28,29]. Accordingly, Mongolian pikas may act as reservoirs for many other infectious agents besides trypanosomes. In light of this, it is very important to increase the number of target species in the family Ochotonidae that are indigenous to Asia, with collection of their ectoparasites, blood, and organ samples from both local animals and introduced wildlife, because these will likely continue to reveal the diversity and distribution of emerging pathogens impacting domestic animals, wildlife, and humans.

In conclusion, this study presents the first evidence of Trypanosoma sp. pika in pikas and fleas and of the B. luni-like genotype in fleas. Our findings expand the taxonomic diversity, geographical range, and host spectrum of Trypanosomatidae. Therefore, it is an important task for future studies to expand the scope of this research by exploring a wider spectrum of natural hosts and arthropod vectors of these blood parasites in Central Asia.