- Open Access
Urban lymphatic filariasis in the metropolis of Dar es Salaam, Tanzania
© Mwakitalu et al.; licensee BioMed Central Ltd. 2013
Received: 17 June 2013
Accepted: 19 September 2013
Published: 30 September 2013
The last decades have seen a considerable increase in urbanization in Sub-Saharan Africa, and it is estimated that over 50% of the population will live in urban areas by 2040. Rapid growth of cities combined with limited economic resources often result in informal settlements and slums with favorable conditions for proliferation of vectors of lymphatic filariasis (LF). In Dar es Salaam, which has grown more than 30 times in population during the past 55 years (4.4 million inhabitants in 2012), previous surveys have indicated high prevalences of LF. This study investigated epidemiological aspects of LF in Dar es Salaam, as a background for planning and implementation of control.
Six sites with varying distance from the city center (3–30 km) and covering different population densities, socioeconomic characteristics, and water, sewerage and sanitary facilities were selected for the study. Pupils from one public primary school at each site were screened for circulating filarial antigen (CFA; marker of adult worm infection) and antibodies to Bm14 (marker of exposure to transmission). Community members were examined for CFA, microfilariae and chronic manifestations. Structured questionnaires were administered to pupils and heads of community households, and vector surveys were carried out in selected households.
The study indicated that a tremendous decrease in the burden of LF infection had occurred, despite haphazard urbanisation. Contributing factors may be urban malaria control targeting Anopheles vectors, short survival time of the numerous Culex quinquefasciatus vectors in the urban environment, widespread use of bed nets and other mosquito proofing measures, and mass drug administration (MDA) in 2006 and 2007. Although the level of ongoing transmission was low, the burden of chronic LF disease was still high.
The development has so far been promising, but continued efforts are necessary to ensure elimination of LF as a public health problem. These will include improving the awareness of people about the role of mosquitoes in transmission of LF, more thorough implementation of environmental sanitation to reduce Cx. quinquefasciatus breeding, continued MDA to high-risk areas, and set-up of programmes for management of chronic LF disease.
The world has witnessed a tremendous increase in urbanization in recent decades. This is also the case for Sub-Saharan Africa, where the urban population has grown from 11.2% in 1950 to 36.3% in 2010 and is estimated to reach more than 50% by the year 2040 . The growth is partly due to migration from rural to urban areas and partly to natural increase in the already existing urban population. Many of the rapid growing cities in developing countries are characterized by insufficient basic infrastructure, and the majority of rural migrants end up in informal and unplanned settlements where basic facilities are poor or absent. In 2010, 62% of the urban population in Sub-Saharan Africa was estimated to live in slums, which are urban areas where households lack access to safe water, adequate sanitation, sufficient living space, durable housing and security of tenure . Such conditions provide favorable habitats for breeding of disease vectors and for transmission of many of the so-called neglected tropical diseases, including lymphatic filariasis (LF), schistosomiasis and soil transmitted helminthiases, which can have severe negative consequences for human health [3–6].
Tanzania has also faced rapid urbanization, with much of it happening in Dar es Salaam, the commercial capital. The population of Dar es Salaam has increased more than 30 times during the past 55 years, from 129,000 in 1957 to 4.4 million in 2012 . Dar es Salaam is currently ranked as the 3rd fastest growing city in Africa and the 9th worldwide . More than 65% of the residents in Dar es Salaam live in un-planned and un-serviced settlements, with poor drinking water and sanitation, poor housing, overcrowding and lack of organized solid waste collection [9–11]. Parts of Dar es Salaam experience flooding during the rains, and it is common to observe clogged open drains, ditches with stagnant household and rain water, and poorly serviced cesspits, septic-tanks and latrines, which support prolific breeding of Culex quinquefasciatus mosquitoes .
Human LF is a disabling and disfiguring disease, which in Sub-Saharan Africa results from infection with the mosquito-borne filarial nematode Wuchereria bancrofti. The vectors responsible for transmission of LF in this region are mainly Anopheles gambiae and An. funestus. However, the relative role of Cx. quinquefasciatus has become increasingly important as a vector in coastal East Africa, particularly in urban and semi-urban environments [13–15]. It is estimated that Sub-Saharan Africa has about 50 million cases of LF, being about one third of the global burden , and Tanzania is ranked the 3rd country in Africa in terms of people at risk (34 million) and people infected (6 million) . Most studies on LF have focused on rural areas, where the burden of infection and disease is highest. This is also the case for Tanzania, where numerous studies have documented high levels of LF endemicity in the rural coastal zone (e.g. [13, 14, 18–20]).
Urban LF has been listed as one of the key challenges in the ongoing global efforts to eliminate LF as a public health problem . Human behavior and culture often differ markedly between rural and urban communities, as do environmental factors that support disease transmission, and simply applying rural control strategies to complex urban settings are not likely to be successful. Instead, strategies are necessary that take the specific behavioral and epidemiological conditions in affected urban environments into consideration. The epidemiology of urban LF has mainly been investigated in large cities in Asia and Brazil . Little is known about urban LF in Sub-Saharan Africa, but two small studies suggested a potential for urban transmission of LF in West Africa [23, 24]. In Dar es Salaam, located on the East African coast, past surveys and spot checks have documented a high prevalence of LF [25–28], and cases of microfilaraemia and clinical manifestations have frequently been noted in clinics and hospitals, but no detailed epidemiological surveys have been carried out. The present study investigated LF infection, disease and transmission in the metropolis of Dar es Salaam, as a background for planning and implementation of control.
Study sites and study populations
Characteristics of the six wards in Ilala District, Dar es Salaam, included in the study
Distance from city centrea
Water and sanitary facilitiesc
Belongs to old part of city centre. Has of late turned into an area with many high business/residential buildings, but also has many poor concrete houses. Busy business area during daytime.
Some parts with piped water from DAWASCOd. Majority rely on bore holes for water. Old underground sewerage system.
Congested unplanned densely populated residential/commercial area, mainly with poor houses. Some small-scale industries. Established as part of the city in the 1940’s.
No central supply of piped water. Majority rely on privately owned bore holes and shallow wells. DAWASCO also has water kiosks.
Congested unplanned densely populated residential/commercial area, mainly with poor houses. Established as industrial and city farming area in 1960’s; later also grew into a residential area.
No central supply of piped water. Private and government owned bore holes, and traditional hand dug shallow wells.
Mixture of houses, from newly well-built to poor mud houses. Established as part of the city in 1970’s.
Small part with piped water from DAWASCO. Majority rely on private bore holes and shallow wells. Buying of water from vendors common.
Unplanned non-surveyed fast-growing new settlement. Most houses good and well built, though there are few poor ones. Established as part of the city in 1990’s.
No central supply of piped water. Private bore holes and hand dug wells common.
Peri-urban. With the rapid expansion of city has recently turned into a residential area. Houses ranging from newly built to poor mud houses. Some small-scale farming.
No central supply of piped water. Private and government owned bore holes. Hand dug wells common. Also water from streams.
The study was carried out from March to October 2011 and had a school part and a community part. In the school part, one (or two neighboring, if necessary) public primary schools were selected from each of the six wards, in order to recruit approximately 300 standard one and two pupils from each ward for examination. The included primary schools (PS) were Mivinjeni and Msimbazi Mseto PS in Mchikichini Ward, Buguruni PS in Buguruni Ward, Mtakuja and Kombo PS in Vingunguti Ward, Mzambarauni PS in Ukonga Ward, Majohe PS in Majohe Ward and Chanika and Tungini PS in Chanika Ward. Lists of the pupils were prepared from the school registers, including their name, age, sex and residence. The parents were asked for written consent before further involvement of their children in the study. Examinations started with a short questionnaire administered to each pupil, after which they were requested to provide finger prick blood samples for determination of circulating filarial antigens (CFA) and antibodies to Bm14.
Four of the six wards from the school part were also included in the community part of the study, namely Mchikichini, Vingunguti, Ukonga and Chanika. In each of these, one ten cell unit (smallest administrative unit in Tanzania; comprises of approximately 10–20 neighboring households, each of which may contain many families), from which many of the examined pupils came, was selected. The local leaders were requested for permission to conduct the study, and the study team and leaders agreed on mode of social mobilization and dates for house to house examination of 250–300 community members aged 10 years and above. Due to frequent reluctance from community members to participate in examinations, the survey team in some cases had to modify the sampling method and also include volunteers from 1–2 neighboring ten cell units. At the time of examination, the community members’ name, age, sex and length of stay in Dar es Salaam was recorded, they provided a finger prick blood sample for determination of CFA, they were clinically examined for chronic manifestations related to LF, and a questionnaire was administered to the heads of households. CFA positive individuals were requested to come to a nearby place at night for blood examination for microfilaraemia. The areas around the four communities were inspected for potential vector breeding sites, and safe water, drainage and sewerage facilities were assessed by using a checklist. Vector surveys were carried out in selected households during the peak mosquito season.
Examination for circulating filarial antigens
Pupils and community members were examined for CFA status by use of Binax NOW Filariasis ICT cards (Inverness Medical Innovations, Maine, USA), according to manufacturers’ instructions and as described [29, 30]. Briefly, 100 μl of finger prick blood was drawn into heparinized tubes and transferred to the pad on the ICT card. The card was closed and the result was read after 10 minutes. A few of the tests were invalid, either because the amount of blood obtained was insufficient or because the blood clotted before reaching the reactive area on the test card.
Examination for antibodies to Bm14
Finger prick blood for examination for antibodies to the recombinant filarial antigen Bm14 was collected from the pupils on TropBio filter paper blood collection discs (TropBio Pty Ltd, Townsville, Australia). Each of the six protrusions on the disc, which have a capacity to absorb 10 μl blood, were saturated with blood from the same pupil. The filter papers were thereafter dried at room temperature for 24 hours, before being placed individually in small zip locked plastic bags and frozen at -20°C. Later, the samples were analyzed in the laboratory for IgG4 antibodies to Bm14 by enzyme linked immunosorbent assay (ELISA) by use of the commercially available CELISA Bm14 kit (Cellabs Pty Ltd, Brokevale, Australia) according to manufacturer’s instructions and as described . Briefly, the samples were thawed and one protrusion from each disc was excised into an Eppendorf tube with 500 μl of sample diluent. The specimens were left in a fridge overnight, and the next morning vortexed to ensure complete extraction of antibodies from the filter paper. From each specimen, 100 μl were transferred to a well in a microtitre plate pre-coated with recombinant Bm14 antigen. Similar volumes of a positive and a negative control from the kit (each in duplicate) and of sample diluent (in six wells; as blanks) were added to wells on the same plate. The plate was incubated for 1 hour at 37°C and thereafter washed four times with PBS/Tween. Enzyme conjugate (100 μl) was added to each well and the plate was incubated at 37°C for 45 minutes. After four washes with PBS/Tween, 100 μl of substrate solution was added to the wells and the plate was incubated in darkness at room temperature for 15 minutes. This was followed by addition of 100 μl of stop solution to each well, after which the optical density (OD) values were read on an ELISA reader (Thermo Scientific Multiskan FC spectrophotometer, Thermo Fischer Scientific Oy, Vantaa, Finland) at dual wavelength of 450/650 nm. OD-values from the positive controls were used to adjust for plate to plate variations. Test specimens with OD-values ≥ 0.4 were considered positive for antibodies to Bm14.
Examination for microfilariae
Blood from community members was examined for microfilariae by using the counting chamber technique, as previously described . Briefly, 100μl of finger prick blood was drawn into a heparinized capillary tube between 22:00 and 02:00 hours and transferred to labelled microtubes with 1.0 ml of 3% acetic acid. In the laboratory the specimens were transferred to a counting chamber, and the microfilariae were counted under a microscope at 40x magnification. To enhance reliability, samples were counted independently by two different individuals and the mean count was used.
During community surveys all study individuals aged 10 years and above were examined for chronic manifestations of LF by a qualified clinician. Manifestations were graded according to size and development stage, but gradings are not used in this presentation.
Surveys for vector mosquitoes
Adult mosquitoes were collected by battery operated CDC light traps (Model 512; John W. Hock Gainesville, USA) in May-July 2011, during the peak mosquito season. Five houses with poor mosquito proofing measures were selected for this purpose in each of the four study wards. During the first week, mosquitoes were collected from Mchikichini and Chanika wards (i.e. 10 light traps) for 5 nights. The following week the exercise was repeated in Vingunguti and Ukonga wards, and so on, until all wards had been sampled for 3 weeks (i.e. total of 15 sampling nights with 5 traps from each ward). All persons sleeping in the collection room were provided with a 2-mm-mesh polyester un-impregnated bed net and the light trap was placed beside one of the occupied bed nets . Traps were switched on at 18:00 hours and off the next morning at 06:00 hours. Caught mosquitoes were transferred to paper cups covered with netting material. Cotton pads soaked in 10% glucose were placed on top of the cups for feeding the mosquitoes. The cups were transported to the laboratory in a cool box. Live mosquitoes were anaesthetized with diethyl ether, the catch was sorted and the mosquitoes identified on morphological characteristics. Live female mosquitoes were dissected under 20x magnification [14, 33]. Wings and legs were removed before head, thorax and abdomen were separated, placed in drops of saline and examined for filarial worms. The mosquitoes were scored as parous or nulliparous after examination of the tracheoles of their ovaries.
Two questionnaires were administered during the study: one to the pupils during the school surveys and the other to heads of household during the community surveys. The questionnaires were in Swahili, and primarily focused on socio-economic, behavioral and environmental factors and on knowledge related to LF and its control.
Research and ethical clearance to carry out the study was granted by the Medical Research Coordinating Committee of the National Institute for Medical Research. Permission to conduct the study was moreover requested from all relevant authorities. Request letters were sent to regional and district medical and education officers, as well as to the schools selected for the study. All schools and communities involved in the study were well informed about the study activities and of the importance of them taking part. Written informed consent was requested from all study participants (with parents consenting for individuals below 18 years). Children who did not want to participate, even after consent from their parents, were excluded from the study.
Data were entered in Excel 2007 and transferred to Stata version 12 for analysis. Geometric mean intensities (GMIs) of Bm14 OD-values were calculated as antilog [(∑logx+1)/n]-1, with x being the OD-values and n the number of individuals included in the calculation. During the community surveys, when only CFA positive individuals were examined for mf, the community mf prevalence was calculated as: (b/a) × (d/c) × 100, where a = number of individuals in the community examined for CFA, b = number of those examined for CFA being positive, c = number of CFA positives examined for mf, and d = number of those examined for mf being positive . Categorical variables were compared statistically by chi-square (χ2) test, whereas continuous variables were compared by t-test or one-way analysis of variance, as appropriate. P-values less than 0.05 were considered statistically significant.
Circulating filarial antigens (CFA) and antibodies to Bm14 as seen in the school study
No. children registered
No. testeda(% of registered)
Mean age in years (range)
No. tests positive (%)
No. tested (% of registered)
No. tests positive (%)
GMIbfor all examined
GMIbfor positives only
Most pupils examined for CFA (95.5%), were also examined for antibodies to Bm14. Among them, 81 (5.0%) tested positive (ward range 2.5-7.4%; Table 2, Figure 2). Prevalences were significantly higher in Majohe (7.4%), Mchikichini (7.0%) and Vingunguti (5.7%) than in the other three wards combined (3.2%; χ2 test, P = 0.004, 0.003 and 0.056, respectively). There was no significant differences in prevalence of antibodies to Bm14 between girls and boys, neither overall (Figure 3) nor in the individual wards (χ2 test, P > 0.05 for all tests). The same pattern was seen for OD-value GMIs calculated on the basis of all examined, with significantly higher mean levels for Majohe, Mchikichini and Vingunguti than for the other three wards combined (t-test, P = 0.02, 0.001 and < 0.001, respectively) and no significant difference between boys and girls (t-test, P > 0.05 for all tests). In all wards, the prevalence of antibodies to Bm14 was higher than the prevalence of CFA.
Questionnaire-based survey in the school study
Were you born in Dar es Salaam?
Does your family own a TV set?
Does your family own a fridge?
Which of these infections is transmitted by mosquitoes (select only one)?
Do you sleep under a bed net?
The knowledge about transmission of the infectious diseases listed in the questionnaire was low (Table 3). Only a few pupils knew that LF was transmitted by mosquitoes (overall 17.0%; ward range 6.2-27.7%), and no clear relationship appeared between ward level of this knowledge and CFA prevalence. The majority of pupils indicated that they slept under a bed net (77.4% always, 15.4% occasionally), and commonly stated that this was mainly due to the mosquito nuisance in their homes. No clear relationship was seen between bed net use and CFA or Bm14 prevalence, neither overall or in individual wards, probably because of the high level of bed net coverage.
Circulating filarial antigens (CFA) and microfilaraemia as seen in the community study
Mean age in years (range)
No. staying ≥ 5 years in the city (%)
No. tests positive (%)
No. positive (%)
Community mf prevalencebin %
Of the 115 individuals found positive for CFA, 99 were also tested for mf (Table 4). Eight of these were mf positive (8.1%; ward range 4.2-13.3%; numbers too low for statistical testing of ward differences). Six of the positives were males and 2 were females (overall mean age 39.3 years, range 13–73 years). As only CFA positives were targeted for mf testing, and as not all of these turned up for night blood sampling, an equation was used to calculate the approximate mf prevalence for the community members on the basis of available data (see Methods). This gave an overall community mf prevalence of 0.8% (ward range 0.4-1.0%; highest mf prevalence in Vingunguti; Figure 4).
Chronic clinical manifestations of lymphatic filariasis as seen in the community study
No. males with hydrocoele (%)
No. females and males with elephantiasisa(%)
Mean age in years (range)
Mean age in years (range)
Questionnaire-based survey among heads of households in the community study
Your main occupation?
Your educational level?
Not completed primary
Completed primary, but not secondary
Average monthly income of your household?
< 80,000 TSh
≥ 80,000 TSh
What causes elephantiasis?
Do not know
What causes hydrocele?
Do not know
Do you sleep under bed net?
Have you ever taken drugs for LF?
Light trap collection of lymphatic filariasis vectors in the community study
No. trap nightsa
No. caught/trap night
The present study examined for LF in a transect of Dar es Salaam, ranging from sites near the center to the semi-rural periphery about 30 km away. It covered localities with a range of different population densities, socioeconomic and environmental characteristics, and water, sewerage and sanitary facilities, being representative for the majority of inhabitants of Dar es Salaam. The more affluent areas (mainly in the center and in the northern part of the city) were not covered, as the likelihood of finding LF infection and disease in these was considered small. The great majority of study individuals were permanent residents in the city, with 99% of pupils indicating they had been born there and 85% of community members indicating they had stayed there for more than 5 years.
As opposed to experience from previous studies carried out by the survey team in rural areas, LF research in the urban settings provided numerous challenges, as also reported by others [21, 35]. Despite great efforts to engage and motivate individuals to take part in the study, the team was frequently met by reluctance and mistrust. In the primary schools, only half of the registered children took part in the study, a major reason being that the parents refused to sign the consent forms. Many parents were not even prepared to discuss advantages and disadvantages of their children’s involvement with the study team. Some parents suspected that the children would be tested for HIV, despite assurance that this would not happen. Some children refused examination despite their parents’ consent, and in Majohe many of the boys ran away each time the survey team came to carry out examinations. These refusals are not likely to have significantly affected the overall prevalence of the measured markers in the children. During the house to house community surveys many individuals also refused to take part, despite involvement of local leaders (including religious) in recruitment. Common excuses were their busy schedule, preconceived ideas relating blood sampling to HIV testing, and requests for payment. During the community surveys in Mchikichini and Vingunguti it was necessary, as a last resort, to change methodology and include volunteers from nearby ten cell units in the survey. This may have attracted a higher than average proportion of individuals with clinical manifestations, in the expectation that they would get some relief from the examinations.
At some of the sites, these events affected the gender balance, with significantly more girls than boys being examined in school surveys in Majohe, and significantly more males than females examined in community surveys in Mchikichini and Vingunguti. In semi-rural Chanika more females than males were included because many males were working more central in the city. As it is well known from most endemic areas that the LF prevalence is higher in males than females [20, 36, 37], especially among adults, the gender balance should be kept in mind when interpreting the findings.
In the school surveys, prevalences of CFA (a marker of adult W. bancrofti infection ) and antibodies to Bm14 (a marker of exposure to W. bancrofti) were generally low, indicating that the level of recent and ongoing LF transmission in the study areas was low. Much higher prevalences of CFA have been reported from Standard 1 pupils in rural endemic areas of coastal Tanzania . The levels of both markers were higher in children from Vingunguti and Majohe than in children from the other examined wards, probably as result of an abundance of waste water ponds, blocked ditches and drains and poor quality pit latrines, favoring breeding of Culicine vectors, in these areas. When using possession of a TV or fridge as indicators of family wealth, pupils from Vingunguti and Majohe moreover came from relatively less wealthy families. Thus, as reported from other urban studies [35, 40–42], there appeared to be an overall trend of higher LF prevalence in urban areas with lower socioeconomic status. A relatively high prevalence of antibodies to Bm14 despite low CFA prevalence in Mchikichini suggests a recent increase in transmission in this ward. It is likely that a major boom in density of Cx. quinquefasciatus during the time of the study , originating from the nearby swampy Msimbazi valley and receiving much public and press attention, had boosted the LF transmission.
Higher infection prevalences were, as expected, seen in the community surveys, but prevalences were still low when compared to the levels commonly seen in endemic rural areas of coastal Tanzania [13, 18–20], and to those reported from Dar es Salaam in the past . Among the four examined wards, the CFA prevalence was again highest in Vingunguti (populated mainly by petty traders with low educational level and income). Very few community individuals had mf in the blood (0.8% overall community prevalence), meaning a limited source of infection for the vectors, and thereby limited transmission. Although LF infection prevalences were relatively low, chronic disease manifestations were common. There was a tendency for chronic disease to be more common in the peripheral wards (Ukonga, Chanika), especially for hydrocele, in particular when considering that in the two more central wards individuals with manifestations may have been attracted to volunteer for examination. The high prevalence of chronic manifestations suggests that infections were previously much more common, and that much of what was seen during the survey was irreversible chronic disease induced in the past. Thus, despite the low level of infection seen, there is still a great need for chronic disease management, especially in the elder part of the community population.
The school study showed no significant difference in CFA prevalence between girls and boys, which is in agreement with previous observations on schoolchildren of same age . Similarly, and interestingly, gender differences in prevalence of antibodies to Bm14 were not seen in these children, suggesting a similar level of exposure among girls and boys in this age group. In the adults, on the other hand, the prevalence of CFA was significantly higher in males than in females, and 6 of the 8 identified mf positive individuals were males. This commonly observed higher LF infection prevalence among adult males has been suggested to stem from behavioral and/or physiological differences between the genders [20, 36, 37]. The present study did not point to gender differences in exposure during childhood as a reason for gender differences in LF infection prevalence in adults.
In addition to suggest an association between LF and low socioeconomic and educational status, the questionnaires indicated that knowledge about LF was generally low at all study sites and among both children and adults. Thus, only 17% of pupils pointed to LF as a mosquito transmitted infection, and only 40% and 29% of adults knew that manifestations of hydrocele and elephantiasis, respectively, were related to mosquito bites. The use of bed nets, on the other hand, was common at all sites among both children and adults, mainly to protect against mosquito nuisance and malaria. On the question of taking drugs for LF (during one or both of the two previous MDAs), the answers among the community members were in sharp contrast to the treatment coverage figures reported for Ilala District by the National LF Elimination Programme (87% of total population in 2006, 74% in 2007), with only 18.6% of them indicating they had taken the drugs. Only 302 (17.8%) and 109 (6.4%) of the 1697 pupils included in the study were 5 years and above during the MDAs in 2007 and 2006, respectively, and could potentially have been treated.
During the vector surveys, large numbers of Cx. quinquefasciatus were caught at all sites, a finding resembling those from other recent surveys in the city [10, 44]. Dar es Salaam has had intensive malaria vector control for many years and very few Anopheles mosquitoes were caught mainly in the periphery of the city. The survey team observed plenty of breeding sites for Cx. quinquefasciatus in the study wards, ranging from waste water pools and blocked open drains to poor quality pit latrines, cesspits and septic tanks, as previously reported . During the rainy season, contaminated spill water was seen in most of the study areas, but particularly in Vingunguti and Mchikichini. The mentioned boom in Cx. quinquefasciatus breeding in the Msimbazi valley during the time of the study resulted in the largest density of these vectors caught in Mchikichini. None of the dissected mosquitoes had infective larvae and only one mosquito was infected with immature larval stages. This may be partly a reflection of the low microfilariae prevalence in the human population, but also of the low age of the vector population. A high proportion of the dissected Cx. quinquefasciatus were nulliparous, meaning they had not yet taken a blood meal. The apparent general short lifespan of these vectors in the urban environment probably makes them rather inefficient in transmitting LF unless they are present in extremely high numbers [41, 45, 46].
LF is well known to occur with high prevalence in rural coastal Tanzania (e.g. [13, 18–20]). Past surveys in Dar es Salaam also indicated high prevalences of LF. Night blood surveys among males in Dar es Salaam showed mf prevalences of 29% , 37%  and 16% , and Minjas and Kihamia  reported mf prevalences in Dar es Salaam to be 15-25% in adults above 30 years. This is in strong contrast to the present study, during which an overall mf prevalence of less than 1% was observed in the adult population. CFA prevalences are generally considerably higher than mf prevalences , but also the CFA prevalences in the present study were low (overall 9.5% for adults and 3.0% for schoolchildren). A number of factors may be responsible for this remarkable decrease: 1. The ongoing Urban Malaria Control Programme has considerably reduced the number of Anopheles mosquitoes, which in addition to being vectors of malaria are also very efficient vectors of LF in Sub-Saharan Africa; 2. Many areas previously covered with surface water, swamps and vegetation, providing habitats for Anopheles sp. breeding, have dried up due to building activities. 3. Cx. quinquefasciatus mosquitoes are breeding profoundly in most areas of Dar es Salaam and are currently the most important vectors of LF in the city. However, these appear to be rather short-lived in the urban environment, and only where they occur in enormous numbers will enough survive long enough to be able to maintain intense and effective transmission. 4. Bed nets are widely used, and together with mosquito screening of houses and generally improved house constructions, have reduced exposure to mosquitoes; 5. MDA with a combination of ivermectin and albendazole was implemented twice (2006 and 2007) in Dar es Salaam by the National LF Elimination Programme, although with many logistic challenges. Thus, despite the fact that rapid urbanization in areas with hot and humid climates has often been seen to sustain or even increase transmission of LF , the overall situation appears to be different in Dar es Salaam, probably due to a favorable combination of the above mentioned factors.
The study indicated that a marked decrease in the burden of LF infection had occurred in the metropolis of Dar es Salaam during recent years, simultaneously with a rapid growth of the population and expansion of areas with poor water, drainage and sanitation facilities. The observed findings are encouraging and give hope that integration of different methods and efforts in fighting LF in urban environments can be successful. However, it was clear from the study that although the burden of infection had decreased, there are still infected individuals and ongoing transmission, in particular in areas where less privileged populations are residing. Improving the awareness among people about the role of mosquitoes in the transmission of LF, more thorough implementation of environmental sanitation to reduce Cx. quinquefasciatus breeding, and continued MDA to high-risk areas, will be important measures to further reduce and perhaps eliminate the transmission of LF. It was also clear from the study that the burden of chronic disease is still considerable, especially in the elderly part of the population, and that facilities and resources for disease management should be made available to those affected. The development has so far been promising, but continuous efforts are necessary to ensure elimination of LF as a public health problem.
The authors are grateful to the pupils and community members for their participation and cooperation and to the school teachers, community leaders and district authorities for their assistance and support. We are also grateful to the technical team from NIMR, Muhimbili (Edward Shogholo and Geofrey Salumu), Muhimbili University of Health and Allied Sciences (Christopher Nfunza, Jordan Megabe and Masumbuko Masunga), NIMR Amani (Demitrius Max) and University of Copenhagen (Jakob Felsager) for skilled assistance in the field and the laboratory. The health officer from Ilala Municipality (Martin Kalongolela) is thanked for performing the clinical examinations. University graduates Coletha Malegere, Chiku Mkamila and Tito Mwakibinga are thanked for assistance in the questionnaire surveys. Grades Stanley (NIMR Headquarters) is thanked for preparing the map. The drivers from NIMR (Sylvester Ngeke, Arison Ekoni and Rodrick Ulomi) are thanked for safe transport. The study received financial support from Danida Research Council, Denmark (FFU grant # 09-096LIFE).
- UN-DESA: World urbanization prospects: The 2011 revision. 2012, New York: United Nations Department of Economic and Social AffairsGoogle Scholar
- UN–HABITAT: State of the World’s Cities 2008/2009: Harmonious cities. 2008, Nairobi: United Nations Human Settlements ProgrammeGoogle Scholar
- Mott KE, Desjeux P, Moncayo A, Ranque P, de Raadt P: Parasitic diseases and urban development. Bull World Health Org. 1990, 68: 691-698.PubMed CentralPubMedGoogle Scholar
- Knudsen AB, Sloof R: Vector-borne disease problems in rapid urbanization: new approaches to vector control. Bull World Health Org. 1992, 70: 1-6.PubMed CentralPubMedGoogle Scholar
- Utzinger J, Keiser J: Urbanization and tropical health - then and now. Ann Trop Med Parasitol. 2006, 100: 517-533. 10.1179/136485906X97372.View ArticlePubMedGoogle Scholar
- Alirol E, Getaz L, Stoll B, Chappuis F, Loutan L: Urbanization and infectious diseases in a globalized world. Lancet Infect Dis. 2011, 11: 131-141. 10.1016/S1473-3099(10)70223-1.View ArticlePubMedGoogle Scholar
- Tanzania NBS: 2012 population and housing census. 2013, Population distribution by administrative areas. Dar es Salaam: National Bureau of StatisticsGoogle Scholar
- City Mayors: The world’s fastest growing cities and urban areas from. 2006,http://www.citymayors.com/statistics/urban_growth1.html, to 2020,
- Chaggu E, Mashauri D, van Buuren J, Sanders W, Lettinga G: Excreta disposal in Dar es Salaam. Env Manag. 2002, 30: 609-620. 10.1007/s00267-002-2685-8.View ArticleGoogle Scholar
- Castro MC, Kanamori S, Kannady K, Mkude S, Killeen GF, Fillinger U: The importance of drains for the larval development of lymphatic filariasis and malaria vectors in Dar es Salaam, United Republic of Tanzania. PLoS Negl Trop Dis. 2010, 4: e693-10.1371/journal.pntd.0000693.PubMed CentralView ArticlePubMedGoogle Scholar
- Penrose K, Castro MC, Werema J, Ryan ET: Informal urban settlements and cholera risk in Dar es Salaam, Tanzania. PLoS Negl Trop Dis. 2010, 4: e631-10.1371/journal.pntd.0000631.PubMed CentralView ArticlePubMedGoogle Scholar
- Simonsen PE: Filariases. Manson’s Tropical Diseases. Edited by: Cook GC, Zumla AI. 2009, London: Saunders Elsevier, 1477-1513. 22Google Scholar
- McMahon JE, Magayuka SA, Kolstrup N, Mosha FW, Bushrod FM, Abaru DE, Bryan JH: Studies on the transmission and prevalence of bancroftian filariasis in four coastal villages of Tanzania. Ann Trop Med Parasitol. 1981, 75: 415-431.PubMedGoogle Scholar
- Rwegoshora RT, Pedersen EM, Mukoko DA, Meyrowitsch DW, Masese N, Malecela–Lazaro MN, Ouma JH, Michael E, Simonsen PE: Bancroftian filariasis: patterns of vector abundance and transmission in two East African communities with different levels of endemicity. Ann Trop Med Parasitol. 2005, 99: 253-265. 10.1179/136485905X29675.View ArticlePubMedGoogle Scholar
- Simonsen PE, Pedersen EM, Rwegoshora RT, Malecela MN, Derua YA, Magesa SM: Lymphatic filariasis control in Tanzania: Effect of repeated mass drug administration with ivermectin and albendazole on infection and transmission. PLoS Negl Trop Dis. 2010, 4: e696-10.1371/journal.pntd.0000696.PubMed CentralView ArticlePubMedGoogle Scholar
- Hotez PJ, Kamath A: Neglected tropical diseases in Sub–Saharan Africa: review of their prevalence, distribution and disease burden. PLoS Negl Trop Dis. 2009, 3: e412-10.1371/journal.pntd.0000412.PubMed CentralView ArticlePubMedGoogle Scholar
- Malecela MN, Lazarus WM, Mwingira U, Mwakitalu E, Makene L, Kabali C, Mackenzie C: Eliminating lymphatic filariasis: a progress report from Tanzania. J Lymph. 2009, 4: 10-12.Google Scholar
- Meyrowitsch DW, Simonsen PE, Makunde WH: Bancroftian filariasis: analysis of infection and disease in five endemic communities of northeastern Tanzania. Ann Trop Med Parasitol. 1995, 89: 653-663.PubMedGoogle Scholar
- Simonsen PE, Meyrowitsch DW, Makunde WH, Magnussen P: Bancroftian filariasis: the pattern of microfilaraemia and clinical manifestations in the three endemic communities in northeastern Tanzania. Acta Trop. 1995, 60: 179-187. 10.1016/0001-706X(95)00126-Y.View ArticlePubMedGoogle Scholar
- Simonsen PE, Meyrowitsch DW, Jaoko WG, Malecela MN, Mukoko D, Pedersen EM, Ouma JO, Rwegoshora RT, Masese N, Magnussen P, Estambale BBA, Michael E: Bancroftian filariasis infection, disease, and specific antibody response patterns in a high and a low endemicity community in East Africa. Am J Trop Med Hyg. 2002, 66: 550-559.PubMedGoogle Scholar
- Addiss D: The 6th meeting of the global alliance to eliminate lymphatic filariasis: A half-time review of lymphatic filariasis elimination and its integration with the control of other neglected tropical diseases. Parasit Vectors. 2010, 3: 100-10.1186/1756-3305-3-100.PubMed CentralView ArticlePubMedGoogle Scholar
- Simonsen PE, Mwakitalu ME: Urban lymphatic filariasis. Parasitol Res. 2013, 112: 35-44. 10.1007/s00436-012-3226-x.PubMed CentralView ArticlePubMedGoogle Scholar
- Gbakima AA, Appawu MA, Dadzie S, Karikali C, Sackey SO, Wilmot AB, Gyapong J, Scott AL: Lymphatic filariasis in Ghana: establishing the potential for an urban cycle of transmission. Trop Med Int Health. 2005, 10: 387-392. 10.1111/j.1365-3156.2005.01389.x.View ArticlePubMedGoogle Scholar
- Terranella A, Eigiege A, Gontor I, Dagwa P, Damishi S, Miri E, Blackburn B, McFarland D, Zingeser J, Jinadu MY, Richards FO: Urban lymphatic filariasis in central Nigeria. Ann Trop Med Parasitol. 2006, 100: 163-172. 10.1179/136485906X86266.View ArticlePubMedGoogle Scholar
- Hawking F: Distribution of filariasis in Tanganyika territory, East Africa. Ann Trop Med Parasitol. 1940, 34: 107-119.Google Scholar
- Jordan P: Filariasis in the Eastern, Tanga and Northern provinces of Tanganyika. E Afri Med J. 1956, 33: 225-233.Google Scholar
- Minjas JN: Filariasis in Tanzania. Proceedings of the Sixth Meeting Organized by the Italian Medical Team, Iringa, Tanzania. 1987, Padova: International College for Health Cooperation in Developing CountriesGoogle Scholar
- Minjas JN, Kihamia CN: Bancroftian filariasis. Health and Diseases in Tanzania. Edited by: Mwaluko GMP, Kilama WL, Mandara MP, Murru M, Macpherson CNL. 1991, London: Harper Collins AcademicGoogle Scholar
- Weil GJ, Lammie PJ, Weiss N: The ICT filariasis test: a rapid-format antigen test for diagnosis of bancroftian filariasis. Parasitol Today. 1997, 13: 401-404. 10.1016/S0169-4758(97)01130-7.View ArticlePubMedGoogle Scholar
- Weil GJ, Ramzy RMR: Diagnostic tools for filariasis elimination programs. Trends in Parasitol. 2007, 23: 78-82. 10.1016/j.pt.2006.12.001.View ArticleGoogle Scholar
- Joseph HM, Melrose W: Applicability of the filter paper technique for detection of antifilarial IgG4 antibodies using the Bm14 filariasis CELISA. J Parasitol Res. 2010, Article 594687Google Scholar
- McMahon JE, de Marshall TF, Vaughan JP, Abaru DE: Bacroftian filariasis: a comparison of microfilariae counting chamber techniques using counting chamber, standard slide and membrane (nuclepore) filtration. Ann Trop Med and Parasitol. 1979, 73: 457-464.Google Scholar
- Pedersen EM, Stolk WA, Laney SJ, Michael E: The role of monitoring mosquito infection in the global programme to eliminate lymphatic filariasis. Trends in Parasitol. 2009, 25: 319-327. 10.1016/j.pt.2009.03.013.View ArticleGoogle Scholar
- Simonsen PE, Derua YA, Kisinza WH, Magesa SM, Malecela MN, Pedersen EM: Lymphatic filariasis control in Tanzania: effects of six rounds of mass drug administration with ivermectin and albendazole on infection and transmission. BMC Infect Dis. 2013, 13: 335-10.1186/1471-2334-13-335.PubMed CentralView ArticlePubMedGoogle Scholar
- Mahfudin H, Partono F, Purmono IKN: Parasitological and clinical survey of bancroftian filariasis in Kepu District, Central Jakarta, Indonesia. Southeast Asian J Trop Med Pub Health. 1977, 8: 173-178.Google Scholar
- Brabin L: Sex differentials in susceptibility to lymphatic filariasis and implications for maternal child immunity. Epidemiol Infect. 1990, 105: 335-353. 10.1017/S0950268800047932.PubMed CentralView ArticlePubMedGoogle Scholar
- Onapa AW, Simonsen PE, Pedersen EM, Okello DO: Lymphatic filariasis in Uganda: baseline investigations in Lira, Soroti and Katakwi districts. Trans R Soc Trop Med Hyg. 2001, 95: 161-167. 10.1016/S0035-9203(01)90145-2.View ArticlePubMedGoogle Scholar
- Joseph H, Maiava F, Naseri T, Taleo F, Ake M, Capuano C, Melrose W: Application of the filariasis CELISA antifilarial IgG4 antibody assay in surveillance in lymphatic filariasis elimination programme in the South Pacific. J Trop Med. 2011, Article ID 492023Google Scholar
- Simonsen PE, Magesa SM, Derua YA, Rwegoshora RT, Malecela MN, Pedersen EM: Monitoring lymphatic filariasis control in Tanzania: effect of repeated mass drug administration on circulating filarial antigen prevalence in young schoolchildren. Int Health. 2011, 3: 182-187. 10.1016/j.inhe.2011.06.009.View ArticlePubMedGoogle Scholar
- Bhattacharya NC, Gubler DJ: A survey for bancroftian filariasis in the Calcutta area. Indian J Med Res. 1973, 61: 8-11.PubMedGoogle Scholar
- Kumar KNV, Ramaiah KD: Usage of personal-protection measures against mosquitoes and the low prevalences of Wuchereria bancrofti microfilaraemia in the Indian city of Chennai. Ann Trop Med Parasitol. 2008, 102: 391-397. 10.1179/136485908X278892.View ArticleGoogle Scholar
- Bonfim C, Alves A, Costa TR, Alencar F, Pedroza D, Portugal JL, Medeiros Z: Spatial analysis and deprivation index to identify urban areas with a high risk of lymphatic filariasis. Trop Med Int Health. 2011, 16: 151-154.View ArticleGoogle Scholar
- Mboera LEG, Kisinza WN, Stanley G, Batengana B, Myamba J: Mosquitoes and mosquito nuisance in Dar es Salaam City, Tanzania. 25th meeting of the National Institute for Medical Research. 2011, Arusha, Tanzania: NIMRGoogle Scholar
- Govella NJ, Chaki PP, Mpangile JM, Killeen GF: Monitoring mosquitoes in urban Dar es Salaam: evaluation of resting boxes, window exit traps, CDC light traps, ifakara tent traps and human landing catches. Parasit Vectors. 2011, 4: 40-10.1186/1756-3305-4-40.PubMed CentralView ArticlePubMedGoogle Scholar
- Joe LK, Hudojo W, Amaliah S: Wuchereria bancrofti infection in Djakarta, Indonesia. A study of some factors influencing its transmission. Indian J Malariol. 1960, 14: 339-352.Google Scholar
- Rajagopalan PK, Mazmi SJ, Mani TR: Some aspects of transmission of Wuchereria bancrofti and ecology of Culex pipiens fatigans in Pondicherry. Indian J Med Res. 1977, 66: 200-215.PubMedGoogle Scholar
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