- Open Access
Characterization of TgPuf1, a member of the Puf family RNA-binding proteins from Toxoplasma gondii
© Liu et al.; licensee BioMed Central Ltd. 2014
Received: 16 February 2014
Accepted: 24 March 2014
Published: 31 March 2014
Puf proteins act as translational regulators and affect many cellular processes in a wide range of eukaryotic organisms. Although Puf proteins have been well characterized in many model systems, little is known about the structural and functional characteristics of Puf proteins in the parasite Toxoplasma gondii.
Using a combination of conventional molecular approaches, we generated endogenous TgPuf1 tagged with hemagglutinin (HA) epitope and investigated the TgPuf1 expression levels and localization in the tachyzoites and bradyzoites. We used RNA Electrophoretic Mobility Shfit Assay (EMSA) to determine whether the recombination TgPuf1 has conserverd RNA binding activity and specificity.
TgPuf1 was expressed at a significantly higher level in bradyzoites than in tachyzoites. TgPuf1 protein was predominantly localized within the cytoplasm and showed a much more granular cytoplasmic staining pattern in bradyzoites. The recombinant Puf domain of TgPuf1 showed strong binding affinity to two RNA fragments containing Puf-binding motifs from other organisms as artificial target sequences. However, two point mutations in the core Puf-binding motif resulted in a significant reduction in binding affinity, indicating that TgPuf1 also binds to conserved Puf-binding motif.
TgPuf1 appears to exhibit different expression levels in the tachyzoites and bradyzoites, suggesting that TgPuf1 may function in regulating the proliferation or/and differentiation that are important in providing parasites with the ability to respond rapidly to changes in environmental conditions. This study provides a starting point for elucidating the function of TgPuf1 during parasite development.
The phylum Apicomplexa consists of single-celled eukaryotic parasites that are responsible for a variety of diseases in humans, pets and farm animals, and are thus of considerable medical and economic importance. Apicomplexan parasites are characterized by complex life cycles usually alternating between sexual and asexual stages involving different hosts. Among these parasites, the best known are Plasmodium falciparum, the causative agent of human malignant malaria and Toxoplasma gondii, responsible for toxoplasmosis in animals and humans. Both of these pathogens have evolved an obligate intracellular lifestyle, with growth, differentiation and replication taking place exclusively inside a protective parasitophorous vacuole within host cells. Unlike Plasmodium, T. gondii can infect a wide range of nucleated cells and differentiate into bradyzoites within tissue cysts that remain latent. Chronic infection with latent bradyzoite cysts is asymptomatic in immunocompetent individuals; however, upon host immunosuppression the parasite reconverts into its proliferative tachyzoite form, which causes severe tissue damage that can result in organ failure and death. Understanding the molecular mechanisms underpinning the conversion of these life stages may identify novel molecular targets for treatment.
Translational control plays a critical role in the regulation of gene expression in most organisms. Compared with transcriptional regulation, translational control of gene expression allows the cell to respond more rapidly to external stimuli. The Puf family RNA-binding proteins (RBPs) modulate mRNA expression in a wide variety of eukaryotic species. PUF proteins execute translation control by binding to specific ribonucleotide sequences called Puf-binding element (PBE), which typically reside in the 3’ untranslated region (3′ UTR) of target mRNAs. The signature feature of the Puf proteins is a highly conserved core RNA-binding domain, referred to as the Puf domain, which almost always contains eight copies of a similar α-helical repeat flanked by one imperfect pseudo-repeat at each end. The Puf domains of Puf proteins from different species are incredibly well conserved, whereas sequences outside the Puf domain vary significantly. The number of Puf genes in each organism is also variable. For example, the Drosophila, human, yeast, and C. elegans genomes encode one, two, six and eleven Puf genes, respectively. While the canonical role of PUFs is translational repression[3, 5], recent evidence suggests that they can contribute to the activation of mRNA expression in some species[6–9]. Furthermore, some have reported that PUFs contribute to the targeting of mRNAs to specific subcellular locations to provide spatial control of expression[10–15]. To date, the functions of Puf proteins have been elucidated during the developmental processes of a number of organisms. Puf proteins have diverse functions, but they appear to share a common, probably ancestral, role in each species that involves promoting proliferation of cells and repressing differentiation. In the protozoan parasite Trypanosoma brucei, Puf1 is essential for cell viability. In Plasmodium, two conserved Puf proteins are preferentially expressed in gametocyte and sporozoite stages[17, 18]. Notably, Puf2 protein appears to play important roles in the stage transition of the malaria parasites. Genetic knockout of the Puf2 gene in P. falciparum and P. berghei promotes differentiation of gametocytes and elevates the male/female sex ratio[19, 20]. In P. berghei sporozoites, Puf2 knockout (KO) parasites experience premature transformation of the sporozoites into forms resembling early intra-hepatic stages while the sporozoites are still inside the salivary glands of the mosquito[19, 21]. Recently, it has been revealed that PfPuf2 regulates the translation of a number of transcripts in gametocytes, including two genes encoding the transmission-blocking vaccine candidates Pfs25 and Pfs28. Altogether, these studies have shed light on the molecular mechanisms by which Puf family proteins regulate mRNA translation.
Translational control contributes to gene regulation in Apicomplexa, particularly in the context of stage differentiation. For instance, the transcript level of bsr4 transcript is equally abundant in both tachyzoites and bradyzoites, but the bsr4 protein is up-regulated only in bradyzoites. Additionally, the phosphorylation of eukaryotic initiation factor-2α, which induces translational control, has been linked to microbial latency in T. gondii. The interesting functions of Puf proteins in regulating stage transition in Plasmodium parasites have prompted us to investigate the Puf homologs in T. gondii. Here, we performed molecular characterization of TgPuf1 in T. gondi and determined its expression, cellular localization and in vitro RNA-binding activity of the recombinant protein. Our results indicate that gene regulation via translational control has an additional level of complexity that involves the 3′UTR in this important group of parasites.
The virulent RH∆Ku80 and avirulent Pru∆Ku80 (Prugniaud) strains of T. gondi were maintained by serial passage in human foreskin fibroblasts (HFF) cultivated in Dulbecco’s modified Eagle medium (DMEM) supplemented with 1% (v/v) heat-inactivated fetal bovine serum (FBS) and 25 μg/L gentamicin antibiotic (Life Technologies). To induce bradyzoite formation, ~50,000 tachyzoites were inoculated onto confluent HFF monolayers in T25 flasks with culture medium. Two to three hours post infection, the culture medium was replaced with a pH 8.2 medium, which was replaced daily.
A total of 47 GenBank entries with complete Puf domains were retrieved for phylogenetic analysis. The Puf domains of TgPufs were trimmed and used to generate the data matrix to infer the phylogenetic relationships among Puf family members. Multiple alignment was performed using the CLUSTALW program (http://www.ebi.ac.uk/clustalw) and the phylogenetic tree was constructed by the neighbor-joining (NJ) method with bootstrap analysis (1000 pseudo-replications) using the MEGA 4 program (http://www.megasoftware.net).
Expression of recombinant TgPuf1 Puf domain in Escherichia coli
To express the conserved RNA-binding domain of TgPuf1 in bacteria, PCR was performed with T. gondii cDNA using two primers (CGGGATCC AGAAAAGGCGACTCAAAAG and ATAAGAATGCGGCCGC GTCACTGAAACCTGAGATG) designed to clone at the Bam HI and Not I sites of the expression vector pGEX-6P-1 (GE Healthcare). The TgPuf1 Puf domain was expressed in E.coli strain BL21 (DE3) as a fusion to the carboxyl-terminus of glutathione S-transferase (GST). Bacteria were grown overnight at 37°C, diluted 1:100 in fresh media and grown to an OD600 value of 0.6. Induction was performed by the addition of 0.1 mM of IPTG and incubated for 4 h. Recombinant protein was purified from 1 L culture using glutathione Sepharose-4B (GE Healthcare) and eluted with 50 mM Tris–HCl (pH 8.0) and 10 mM reduced glutathione. Purified recombinant TgPuf1 (rTgPuf1) protein was dialyzed extensively in phosphate-buffered saline (PBS, pH 7.0) and used for immunization in rabbits for antibodies and for in vitro RNA binding assay.
Plasmid construction and parasite transfection
A Toxoplasma clone stably expressing TgPuf1 tagged at its C-terminus with the 3X hemagglutinin (HA) epitope was generated by targeting the endogenous TgPuf1 locus using homologous recombination. RH∆Ku80 genomic DNA was used to amplify a 1.3-kb fragment of the Puf1 3′ end using primers Puf1HA_F (5′-TACTTCCAATCCAATTTAATGC GTATGCGAACTATGGTAAGACT-3′) and Puf1HA_R (5′-TCCTCCACTTCCAATTTTAGC CATCCCATCGACAGCAATC-3′) that contained ligation-independent cloning sequences (italics). This Puf1 fragment was inserted into the pLIC_HAx3_DHFRTs endogenous tagging vector such that the TgPuf1 coding sequence was fused in frame with the epitope coding region. The pLIC_Puf1HAx3_DHFRTs construct was confirmed by sequencing. For transfection, 30 μg of the pLIC_Puf1HAx3_DHFRTs plasmid was linearized by overnight digestion with Blp I within the Puf1 homologous region and ethanol precipitated. RH∆Ku80 and Pru∆Ku80 tachyzoites were transformed with the linearized construct by electroporation, and after overnight growth in HFF, parasite cultures were selected with 1.0 μM pyrimethamine. Drug-resistant parasites were cloned by limiting dilution and screened by Western blot and immunofluorescence for expression of HA-tagged TgPuf1.
To study TgPuf1 protein expression, equal amounts of the parasite lysates (25 μg) of tachyzoites and bradyzoites were separated by SDS/PAGE (8%) and transferred to nitrocellulose membranes. Bradyzoites were induced by alkaline-stress for 12 days. To isolate bradyzoites, infected cells were scraped from the flask and passed through an 18G needle for 10 times, and bradyzoites were purified from host cell debris by filtration through a 25 mm Nuclepore Track-Etched Polycarbonate Membrane circle with a 3.0 μm pore size (GE Healthcare) into a conical tube. The parasites were pelleted by centrifugation and washed with cold PBS at 4°C. Western blot was carried out using rat anti-HA antibodies (Roche) (1:2,000) or rabbit anti-rTgPuf1 antiserum (1,1000) as the primary antibodies and horseradish peroxidase-conjugated goat anti-rabbit or anti-rat IgG (1:3,000) as the secondary antibodies. Antibodies to Toxoplasma BAG1 (1:1,000) were used to detect protein expression in bradyzoites. Toxoplasma β-tubulin expression detected by specific polyclonal antibodies (1:1,000) served as a protein loading control. The results were visualized with the ECL detection system (GE Healthcare). The density of bands detected in Western blot was analyzed by ImageJ software and normalized with the β-tubulin loading control as the ratio of TgPuf1/β-tubulin. This experiment was repeated three times, and the expression levels of TgPuf1 between bradyzoites and tachyzoites were compared by T-test.
Indirect Immunofluorescent assay (IFA)
For IFA, infected HFF monolayers grown on coverslips were fixed in 4% paraformaldehyde for 20 min at room temperature. They were then permeablized for 10 min in PBS containing 3% BSA and 0.2% Triton X-100 and blocked for 1 h in PBS with 3% BSA. They were first probed with rabbit anti-HA antibody (Sigma) (1:500) and anti-BAG1 antibodies (1:100). Secondary antibodies were FITC-labeled anti-rabbit IgG (Sigma) and TRITC-labeled anti-mouse IgG (Sigma). Fluorescent images were obtained with a Nikon ECLIPSE E600 epifluorescence microscope.
In vitro RNA binding assay
Electrophoretic mobility shift assay (EMSA) was performed using the Light Shift Chemiluminescent RNA EMSA kit (Pierce). Briefly, each 20 μl of reaction contained 2 μg tRNA for blocking non-specific RNA-protein interactions, EMSA binding buffer, 20 units of RNase inhibitor, 5% glycerol, rTgPuf1, and biotinylated RNA oligos with or without cold competitors. The artificial Puf target RNAs included the Drosophila hunchback (hb) Nanos Response Element (NRE) sequence AUUAUUUUGUUGU CGAAAAUUGU ACAUAAGCC and the pfs28 3′ UTR sequence (Pfs28 RNA1) GAAAUGUUCUUUUGUAAUUA UAUUUUGUUCGAUGAUUC, where the PBEs essential for Puf binding are in italics. A Pfs28 RNA1Moligo, in which the UGU sequence in the PBE of Pfs28 RNA1 was mutated to UCC, was used to determine whether this would interfere with TgPuf1 binding. These oligos were synthesized as biotin-labeled RNA fragments (Integrated DNA Technologies). In a 20 μl reaction, 2.5 nM of an RNA oligo and different concentrations of rTgPuf1 (0.78 – 400 nM) were incubated at room temperature for 20 min. Cold competitor (unlabeled) RNAs were included at 5 X, 50 X and 100 X concentrations of the biotinylated RNAs to demonstrate binding specificity. The reactions were electrophoresed on a 5% native acrylamide/8 M urea gel and transferred to a nylon membrane. The bands of labeled oligos were detected using the Chemilumescent Nucleic Acid Detection Module (Pierce). Each experiment was repeated three times and the average Kd values were estimated by fitting the curves to the mean percentages of the total bound RNA, which were determined by densitometry using the Quantity One 1-D Analysis Software (BioRad).
Toxoplasma encodes two putative Puf proteins
Expression of the recombinant TgPuf1 Puf domain
TgPuf1 is expressed in both tachyzoite and bradyzoite
Subcellular localization of TgPuf1
IFA with anti-HA antibodies detected TgPuf1 protein in the cytoplasm, consistent with its function in translation control. In bradyzoites induced by alkaline-stress, the TgPuf1 protein showed a much more granular cytoplasmic staining pattern. Such punctate cytoplasmic structures were more obvious in bradyzoites (Figure 4C, Additional file2: Figure S2), whereas they had a relatively uniform distributionin the cytoplasm of tachyzoites (Figure 4C, Additional file2: Figure S2).
In vitro binding activity of the rTgPuf1
Translational regulation of gene expression plays an important role in the development of diverse eukaryotes. In many cases, post-transcriptional regulation requires cis-acting sequences located in either the 3′ or 5′ UTRs of the transcript. We have shown here that T.gondii possesses two distinct Puf members, which share limited sequence similarity, suggesting they might regulate different RNA repertoires and have different functions. Interestingly, TgPuf1 and TgPuf2 are more homologous to their respective Puf1 and Puf2 genes in Plasmodium, suggesting that the duplication of Puf genes in these two Apicomplexan parasites occurred earlier before the divergence of these parasite taxa. In the malaria parasite P. berghei, only PbPuf2 are found to regulate the stage-transition in sporozoites, whereas deletion of PbPuf1 had no effects on this process[1, 2, 19, 28]. In P. falciparum both Puf proteins are abundantly expressed in gametocytes and Puf2 plays an important role during gametocytogenesis. In Toxoplasma, TgPuf1 appeared to be more abundantly expressed in bradyzoites at the protein level, suggesting that TgPuf1 protein may also function during the tachyzoite-bradyzoite transformation. Future study will be directed to decipher the potential role of TgPuf1 in regulating stage transition through gene disruption analysis.
The PUF domain contains eight PUM repeats, each containing three α-helices packed together in a curved structure. RNA is bound as an extended strand to the concave surface of the PUF domain with the bases contacted by protein side chains. Specifically, the eight bases of the target RNA, 1–8, are contacted by protein repeats 8–1, with the critical UGU sequence recognized by repeats 8, 7 and 6, respectively. Here we showed that the rTgPuf1 PUM domain has the conserved RNA binding activity to canonical target RNAs and the binding depends on the presence of the essential UGU motif. In line with other reports, mutations in the UGUR sequence abolishes or significantly interferes with the binding[30, 31, 34–38]. A search of the Toxoplasma genome for the presence of the PBE sequence identified 130,571 UGUX3UA motifs, which remain to be determined as Puf binding targets.
In accordance with the primary role of Puf proteins, Puf proteins are predominantly localized within the cytoplasm of cells. Two exceptions are T. brucei Puf7, which is localized in the nucleolus, and S. cerevisiae Puf6p, which is present in both the cytoplasm and nucleus. TgPuf1 is localized in the cytoplasm and it forms punctate cytoplasmic structures in bradyzoites. These structures are reminiscent of “stress granules” or “processing bodies (p-bodies)” formed upon exposure of the cells to stress conditions[41, 42]. Stress granules are large cytoplasmic aggregates, where mRNAs stalled at translation initiation are stored. They contain numerous RBPs, mRNA, the 40S ribosomal subunit and a number of initiation factors[41, 42]. Whereas stress granules are rarely found in growing cells, they are induced rapidly after exposure to many types of stress. P-bodies are typically found in growing cells, however, they become larger and more numerous upon exposure to stress, and can be observed to physically interact with stress granules[41, 42]. Stress granules and P-bodies have been found to contain a large number of RBPs, including Puf proteins[41–43]. Given the localization of human PUM1 and PUM1 to cytoplasmic stress granules, the punctate staining patterns of TgPuf1 suggest that they might be localized to similar granules, although it still requires co-localization confirmation with a marker for the stress granules or P-bodies[10, 44]. Interestingly, several target mRNAs protected from translation and degradation in the P. berghei gametocytes are bound to the DOZI RNA helicase complex, which is apparently devoid of Puf proteins[45, 46]. Future work is necessary to elucidate the biological roles, spatial and temporal regulation, interaction partners, and regulated biological pathways of TgPuf proteins.
We have shown here that TgPuf1 has conserved RNA-binding activity and specificity towards the Puf-binding elements. It appears to be expressed differentially in tachyzoites and bradyzoites, suggesting that TgPuf1 may function in regulating the proliferation or/and differentiation, which might be important in providing parasites with the ability to respond rapidly to changes of environmental conditions.
Min Liu is supported by a scholarship from Overseas Study Program of Guangzhou Elite Project. This study is supported by a grant from National Nature Science Foundation of China (No.31030066) to XG Chen. We are grateful to Dr. David Sibley (Washington University, St. Louis, MO) for providing the anti-β-tubulin antibodies and Dr. Louis Weiss (Albert Einstein College of Medicine, NY) for the antibodies to BAG1and Pru∆Ku80 parasite strain. Research in the Sullivan lab is supported by grants from the NIH (AI077502 and AI105786).
- Sullivan WJ, Jeffers V: Mechanisms of Toxoplasma gondii persistence and latency. FEMS Microbiol Rev. 2012, 36: 717-733. 10.1111/j.1574-6976.2011.00305.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Curtis D, Lehmann R, Zamore PD: Translational regulation in development. Cell. 1995, 81: 171-178. 10.1016/0092-8674(95)90325-9.View ArticlePubMedGoogle Scholar
- Wickens M, Bernstein DS, Kimble J, Parker R: A PUF family portrait: 3′UTR regulation as a way of life. Trends Genet. 2002, 18: 150-157. 10.1016/S0168-9525(01)02616-6.View ArticlePubMedGoogle Scholar
- Spassov DS, Jurecic R: Cloning and comparative sequence analysis of PUM1 and PUM2 genes, human members of the Pumilio family of RNA-binding proteins. Gene. 2002, 299: 195-204. 10.1016/S0378-1119(02)01060-0.View ArticlePubMedGoogle Scholar
- Wharton RP, Aggarwal AK: mRNA regulation by Puf domain proteins. Sci STKE. 2006, 2006: e37.View ArticleGoogle Scholar
- Pique M, Lopez JM, Foissac S, Guigo R, Mendez R: A combinatorial code for CPE-mediated translational control. Cell. 2008, 132: 434-448. 10.1016/j.cell.2007.12.038.View ArticlePubMedGoogle Scholar
- Kaye JA, Rose NC, Goldsworthy B, Goga A, L’Etoile ND: A 3′UTR pumilio-binding element directs translational activation in olfactory sensory neurons. Neuron. 2009, 61: 57-70. 10.1016/j.neuron.2008.11.012.PubMed CentralView ArticlePubMedGoogle Scholar
- Archer SK, Luu VD, de Queiroz RA, Brems S, Clayton C: Trypanosoma brucei PUF9 regulates mRNAs for proteins involved in replicative processes over the cell cycle. PLoS Pathog. 2009, 5: e1000565-10.1371/journal.ppat.1000565.PubMed CentralView ArticlePubMedGoogle Scholar
- Suh N, Crittenden SL, Goldstrohm A, Hook B, Thompson B, Wickens M, Kimble J: FBF and its dual control of gld-1 expression in the Caenorhabditis elegans germline. Genetics. 2009, 181: 1249-1260. 10.1534/genetics.108.099440.PubMed CentralView ArticlePubMedGoogle Scholar
- Vessey JP, Vaccani A, Xie Y, Dahm R, Karra D, Kiebler MA, Macchi P: Dendritic localization of the translational repressor Pumilio 2 and its contribution to dendritic stress granules. J Neurosci. 2006, 26: 6496-6508. 10.1523/JNEUROSCI.0649-06.2006.View ArticlePubMedGoogle Scholar
- Saint-Georges Y, Garcia M, Delaveau T, Jourdren L, Le Crom S, Lemoine S, Tanty V, Devaux F, Jacq C: Yeast mitochondrial biogenesis: a role for the PUF RNA-binding protein Puf3p in mRNA localization. PLoS One. 2008, 3: e2293-10.1371/journal.pone.0002293.PubMed CentralView ArticlePubMedGoogle Scholar
- Deng Y, Singer RH, Gu W: Translation of ASH1 mRNA is repressed by Puf6p-Fun12p/eIF5B interaction and released by CK2 phosphorylation. Genes Dev. 2008, 22: 1037-1050. 10.1101/gad.1611308.PubMed CentralView ArticlePubMedGoogle Scholar
- Zipor G, Haim-Vilmovsky L, Gelin-Licht R, Gadir N, Brocard C, Gerst JE: Localization of mRNAs coding for peroxisomal proteins in the yeast, Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2009, 106: 19848-19853. 10.1073/pnas.0910754106.PubMed CentralView ArticlePubMedGoogle Scholar
- Eliyahu E, Pnueli L, Melamed D, Scherrer T, Gerber AP, Pines O, Rapaport D, Arava Y: Tom20 mediates localization of mRNAs to mitochondria in a translation-dependent manner. Mol Cell Biol. 2010, 30: 284-294. 10.1128/MCB.00651-09.PubMed CentralView ArticlePubMedGoogle Scholar
- Vessey JP, Schoderboeck L, Gingl E, Luzi E, Riefler J, Di Leva F, Karra D, Thomas S, Kiebler MA, Macchi P: Mammalian Pumilio 2 regulates dendrite morphogenesis and synaptic function. Proc Natl Acad Sci U S A. 2010, 107: 3222-3227. 10.1073/pnas.0907128107.PubMed CentralView ArticlePubMedGoogle Scholar
- Hoek M, Zanders T, Cross GA: Trypanosoma brucei expression-site-associated-gene-8 protein interacts with a Pumilio family protein. Mol Biochem Parasitol. 2002, 120: 269-283. 10.1016/S0166-6851(02)00009-9.View ArticlePubMedGoogle Scholar
- Cui L, Fan Q, Li J: The malaria parasite Plasmodium falciparum encodes members of the Puf RNA-binding protein family with conserved RNA binding activity. Nucleic Acids Res. 2002, 30: 4607-4617. 10.1093/nar/gkf600.PubMed CentralView ArticlePubMedGoogle Scholar
- Fan Q, Li J, Kariuki M, Cui L: Characterization of PfPuf2, member of the Puf family RNA-binding proteins from the malaria parasite Plasmodium falciparum. DNA Cell Biol. 2004, 23: 753-760. 10.1089/dna.2004.23.753.View ArticlePubMedGoogle Scholar
- Muller K, Matuschewski K, Silvie O: The Puf-family RNA-binding protein Puf2 controls sporozoite conversion to liver stages in the malaria parasite. PLoS One. 2011, 6: e19860-10.1371/journal.pone.0019860.PubMed CentralView ArticlePubMedGoogle Scholar
- Miao J, Fan Q, Parker D, Li X, Li J, Cui L: Puf mediates translation repression of transmission-blocking vaccine candidates in malaria parasites. PLoS Pathog. 2013, 9: e1003268-10.1371/journal.ppat.1003268.PubMed CentralView ArticlePubMedGoogle Scholar
- Gomes-Santos CS, Braks J, Prudencio M, Carret C, Gomes AR, Pain A, Feltwell T, Khan S, Waters A, Janse C, Mair GR, Mota MM: Transition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilio. PLoS Pathog. 2011, 7: e1002046-10.1371/journal.ppat.1002046.PubMed CentralView ArticlePubMedGoogle Scholar
- Zhang M, Joyce BR, Sullivan WJ, Nussenzweig V: Translational control in Plasmodium and toxoplasma parasites. Eukaryot Cell. 2013, 12: 161-167. 10.1128/EC.00296-12.PubMed CentralView ArticlePubMedGoogle Scholar
- Van TT, Kim SK, Camps M, Boothroyd JC, Knoll LJ: The BSR4 protein is up-regulated in Toxoplasma gondii bradyzoites, however the dominant surface antigen recognised by the P36 monoclonal antibody is SRS9. Int J Parasitol. 2007, 37: 877-885. 10.1016/j.ijpara.2007.02.001.View ArticlePubMedGoogle Scholar
- Konrad C, Queener SF, Wek RC, Sullivan WJ: Inhibitors of eIF2alpha dephosphorylation slow replication and stabilize latency in Toxoplasma gondii. Antimicrob Agents Chemother. 2013, 57: 1815-1822. 10.1128/AAC.01899-12.PubMed CentralView ArticlePubMedGoogle Scholar
- Zhang G, Huang X, Boldbaatar D, Battur B, Battsetseg B, Zhang H, Yu L, Li Y, Luo Y, Cao S, Goo YK, Yamagishi J, Zhou J, Zhang S, Suzuki H, Igarashi I, Mikami T, Nishikawa Y, Xuan X: Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice. Vaccine. 2010, 28: 7243-7247. 10.1016/j.vaccine.2010.08.096.View ArticlePubMedGoogle Scholar
- Wang X, McLachlan J, Zamore PD, Hall TM: Modular recognition of RNA by a human pumilio-homology domain. Cell. 2002, 110: 501-512. 10.1016/S0092-8674(02)00873-5.View ArticlePubMedGoogle Scholar
- Lu G, Dolgner SJ, Hall TM: Understanding and engineering RNA sequence specificity of PUF proteins. Curr Opin Struct Biol. 2009, 19: 110-115. 10.1016/j.sbi.2008.12.009.PubMed CentralView ArticlePubMedGoogle Scholar
- Gerber AP, Luschnig S, Krasnow MA, Brown PO, Herschlag D: Genome-wide identification of mRNAs associated with the translational regulator PUMILIO in Drosophila melanogaster. Proc Natl Acad Sci U S A. 2006, 103: 4487-4492. 10.1073/pnas.0509260103.PubMed CentralView ArticlePubMedGoogle Scholar
- Tam PP, Barrette-Ng IH, Simon DM, Tam MW, Ang AL, Muench DG: The Puf family of RNA-binding proteins in plants: phylogeny, structural modeling, activity and subcellular localization. BMC Plant Biol. 2010, 10: 44-10.1186/1471-2229-10-44.PubMed CentralView ArticlePubMedGoogle Scholar
- Zamore PD, Williamson JR, Lehmann R: The Pumilio protein binds RNA through a conserved domain that defines a new class of RNA-binding proteins. RNA. 1997, 3: 1421-1433.PubMed CentralPubMedGoogle Scholar
- White EK, Moore-Jarrett T, Ruley HE: PUM2, a novel murine puf protein, and its consensus RNA-binding site. RNA. 2001, 7: 1855-1866.PubMed CentralPubMedGoogle Scholar
- Miao J, Li J, Fan Q, Li X, Li X, Cui L: The Puf-family RNA-binding protein PfPuf2 regulates sexual development and sex differentiation in the malaria parasite Plasmodium falciparum. J Cell Sci. 2010, 123: 1039-1049. 10.1242/jcs.059824.PubMed CentralView ArticlePubMedGoogle Scholar
- Miller MA, Olivas WM: Roles of Puf proteins in mRNA degradation and translation. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2011, 2: 471-492.View ArticleGoogle Scholar
- Murata Y, Wharton RP: Binding of pumilio to maternal hunchback mRNA is required for posterior patterning in Drosophila embryos. Cell. 1995, 80: 747-756. 10.1016/0092-8674(95)90353-4.View ArticlePubMedGoogle Scholar
- Sonoda J, Wharton RP: Recruitment of Nanos to hunchback mRNA by Pumilio. Genes Dev. 1999, 13: 2704-2712. 10.1101/gad.13.20.2704.PubMed CentralView ArticlePubMedGoogle Scholar
- Bernstein D, Hook B, Hajarnavis A, Opperman L, Wickens M: Binding specificity and mRNA targets of a C. elegans PUF protein, FBF-1. RNA. 2005, 11: 447-458. 10.1261/rna.7255805.PubMed CentralView ArticlePubMedGoogle Scholar
- Padmanabhan K, Richter JD: Regulated Pumilio-2 binding controls RINGO/Spy mRNA translation and CPEB activation. Genes Dev. 2006, 20: 199-209. 10.1101/gad.1383106.PubMed CentralView ArticlePubMedGoogle Scholar
- Muraro NI, Weston AJ, Gerber AP, Luschnig S, Moffat KG, Baines RA: Pumilio binds para mRNA and requires Nanos and Brat to regulate sodium current in Drosophila motoneurons. J Neurosci. 2008, 28: 2099-2109.PubMed CentralView ArticlePubMedGoogle Scholar
- Droll D, Archer S, Fenn K, Delhi P, Matthews K, Clayton C: The trypanosome Pumilio-domain protein PUF7 associates with a nuclear cyclophilin and is involved in ribosomal RNA maturation. FEBS Lett. 2010, 584: 1156-1162. 10.1016/j.febslet.2010.02.018.PubMed CentralView ArticlePubMedGoogle Scholar
- Gu W, Deng Y, Zenklusen D, Singer RH: A new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization. Genes Dev. 2004, 18: 1452-1465. 10.1101/gad.1189004.PubMed CentralView ArticlePubMedGoogle Scholar
- Anderson P, Kedersha N: RNA granules. J Cell Biol. 2006, 172: 803-808. 10.1083/jcb.200512082.PubMed CentralView ArticlePubMedGoogle Scholar
- Anderson P, Kedersha N: Stress granules: the Tao of RNA triage. Trends Biochem Sci. 2008, 33: 141-150. 10.1016/j.tibs.2007.12.003.View ArticlePubMedGoogle Scholar
- Kedersha N, Stoecklin G, Ayodele M, Yacono P, Lykke-Andersen J, Fritzler MJ, Scheuner D, Kaufman RJ, Golan DE, Anderson P: Stress granules and processing bodies are dynamically linked sites of mRNP remodeling. J Cell Biol. 2005, 169: 871-884.PubMed CentralView ArticlePubMedGoogle Scholar
- Morris AR, Mukherjee N, Keene JD: Ribonomic analysis of human Pum1 reveals cis-trans conservation across species despite evolution of diverse mRNA target sets. Mol Cell Biol. 2008, 28: 4093-4103. 10.1128/MCB.00155-08.PubMed CentralView ArticlePubMedGoogle Scholar
- Mair GR, Braks JA, Garver LS, Wiegant JC, Hall N, Dirks RW, Khan SM, Dimopoulos G, Janse CJ, Waters AP: Regulation of sexual development of Plasmodium by translational repression. Science. 2006, 313: 667-669. 10.1126/science.1125129.PubMed CentralView ArticlePubMedGoogle Scholar
- Mair GR, Lasonder E, Garver LS, Franke-Fayard BM, Carret CK, Wiegant JC, Dirks RW, Dimopoulos G, Janse CJ, Waters AP: Universal features of post-transcriptional gene regulation are critical for Plasmodium zygote development. PLoS Pathog. 2010, 6: e1000767-10.1371/journal.ppat.1000767.PubMed CentralView ArticlePubMedGoogle Scholar
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