Open Access

Re-emergence of bovine piroplasmosis in Hungary: has the etiological role of Babesia divergens been taken over by B. major and Theileria buffeli?

  • Sándor Hornok1Email author,
  • Anita Mester2,
  • Nóra Takács1,
  • Isabel G Fernández de Mera3,
  • José de la Fuente3, 4 and
  • Róbert Farkas1
Parasites & Vectors20147:434

https://doi.org/10.1186/1756-3305-7-434

Received: 29 May 2014

Accepted: 4 September 2014

Published: 11 September 2014

Abstract

Background

The prevalence of bovine babesiosis caused by Babesia divergens has been declining during the past decades in northeastern Hungary, and no cases have been observed since 2008. Infections of cattle with B. major and Theileria buffeli were hitherto reported in southern and western Europe. In other parts of the globe, there is evidence of emergence and a growing clinical importance of T. buffeli and closely related genotypes of the T. orientalis complex.

Findings

In a herd of 88 beef cattle kept in northeastern Hungary, bovine piroplasmosis was diagnosed in nine animals through the examination of blood smears or by molecular methods. B. major was identified in five animals, two of which died. In addition, four cattle harboured T. buffeli, and one of these animals was anaemic. Despite their presence, a contributory role of Anaplasma marginale and A. phagocytophilum could not be established in the disease cases.

Conclusions

In this study B. major and bovine theileriosis is reported for the first time in central-eastern Europe, where clinical cases were associated with a mild winter.

Keywords

Cattle Babesiosis Theileriosis Anaplasma marginale Anaplasma ovis A. phagocytophilum

Findings

Background

Bovine piroplasmosis is caused by Babesia and Theileria spp. which are transmitted by ixodid ticks as biological vectors [1, 2]. The geographical ranges of babesioses and theilerioses are defined by the region where competent vectors are indigenous.

Thus, Babesia divergens, which is zoonotic and regarded as highly pathogenic to cattle, is widespread in Europe, owing to its vector tick species Ixodes ricinus[1]. However, despite its clinical importance, this piroplasm has lost its significance over time in some countries. In northeastern Hungary, the last case of known clinical B. divergens infection was diagnosed in May 2008 (GenBank: HQ395757, unpublished observations). Prior to that, during the past decades, a gradual decline was noted in the number of disease cases caused by this piroplasm until it became virtually extinct [3]. This phenomenon, i.e., the decreasing prevalence of clinical B. divergens infections, was also shown in Ireland [4] and Norway [5].

On the other hand, B. major and Theileria buffeli, which are usually considered benign agents, have been reported from a small number of countries in Europe, including the UK, the Netherlands, France, Germany and Spain for B. major[69] and the UK, Portugal, Spain, Italy and Greece for T. buffeli[913]. Altogether, countries endemic for both piroplasms appear to be confined to southern and western Europe, corresponding to the regions where their common vector, Haemaphysalis punctata occurs [14].

While B. major will mostly cause either mild disease or no clinical signs, this species has also been implicated in cases of bovine anaemia (with haematocrit values decreasing to 17%) and haemoglobinuria [6, 9]. Similarly, T. buffeli may cause anaemia, oedema, icterus and recumbency in cattle [10, 15, 16]. During the past few years there has been growing concern about the increased involvement of T. buffeli and other genotypes of the T. orientalis complex in clinical cases, implying the emergence of bovine theileriosis on other continents [16, 17].

Here we report accumulated cases of mild to fatal piroplasmosis in a beef cattle herd in northeastern Hungary with unusual seasonality and causative agents (B. major, T. buffeli) that are new in the central-eastern European region. Because bovine anaplasmosis could have been important in the differential diagnoses (Anaplasma marginale and A. ovis occur in the same county in Hungary [18], and A. phagocytophilum is similarly known to cause anaemia [19]), the presence of these Anaplasma spp. was also evaluated in the herd.

Methods

The first cattle, which had been grazing the same pastures in northeastern Hungary (Nógrád county, Mátranovák, geographical coordinates: 48° 2' 12.4" N, 19° 58' 35.1" E) in 2012–2013, exhibited clinical signs of piroplasmosis in November, 2013. In the same month, a herd of 88 Charolais beef cattle were transferred from southeastern to northeastern Hungary, to the above locality. In the latter herd, clinical signs of piroplasmosis were noted in two animals within 1.5 months of their arrival, in December, 2013. Relevant animals were kept on pasture in the same area throughout the year.

After the appearance of clinical signs, EDTA blood samples were collected from the coccygeal vein of the three severely affected animals, and the presence of piroplasms was evaluated in Giemsa-stained blood smears. In one animal, complete haematological and biochemical analyses were also performed (Hemo-Vet, Veterinary Clinical Laboratory, Budapest, Hungary). EDTA blood samples were collected from all 85 cattle in the herd in January, 2014. In addition, haematological analysis was performed in blood samples in which piroplasms were identified by molecular methods.

DNA was extracted with the QIAamp Mini Kit (QIAGEN, Hilden, Germany) individually from 200 μl of 85 blood samples, as reported previously [20]. Subsequently, all PCRs were run with the appropriate positive and negative controls. All blood DNA samples were screened for the presence of piroplasms by conventional PCR [21]. In brief, the primers BJ1 (forward: 5'-GTC TTG TAA TTG GAA TGA TGG-3') and BN2 (reverse: 5'-TAG TTT ATG GTT AGG ACT ACG-3') were used to amplify an approximately 500 bp portion of the 18S rRNA gene of Babesia/Theileria spp. The reaction volume was 25 μl, i.e., 5 μl of extracted DNA was added to 20 μl of a reaction mixture containing 0.5 U HotStarTaq DNA Plus polymerase (5U/ μl), 200 μM of PCR nucleotide mix, 1 μM of each primer and 2.5 μl of 10× Coral Load PCR buffer (15 mM MgCl2 included). Cycling conditions included an initial denaturation step at 95°C for 10 min, followed by 40 cycles of denaturation at 95°C for 30 s, annealing at 54°C for 30 s and extension at 72°C for 40 s. The final extension was performed at 72°C for 5 min. Purification and sequencing of PCR products was performed by Biomi Inc. (Gödöllő, Hungary). Representative sequences were submitted to GenBank (KJ756504 for B. major, KJ756505 for T. buffeli).

In addition, all cattle blood DNA samples were evaluated for the presence of Anaplasma spp. Anaplasma phagocytophilum was investigated with a TaqMan real-time PCR that amplifies part of the major surface protein 2 (msp2) gene (as described in [20]). Analysis for A. marginale was carried out in two steps: first by screening for Anaplasmataceae members by a 16S rRNA gene conventional PCR (as described in [18]), followed by a conventional PCR for the msp4 gene of A. marginale/A. ovis according to [22]. Sequencing of the cloned product of the latter test was attempted from all animals that were Babesia or Theileria infected. Obtained sequences were submitted to GenBank (KJ883270-71).

Confidence intervals (CI) for the prevalence rates were calculated at the level of 95%.

Results

In November and December, three cattle (No. 1–3) showed clinical signs of babesiosis (Table 1), and B. major was detected in their blood smears. No Theileria or Anaplasma inclusion bodies were seen in the red blood cells. Clinical laboratory findings from animal No. 1 (Table 1) were suggestive of pathological changes in parenchymal organs, muscle damage and chronic haemolysis. Despite treatment with 1 ml Imizol® (Intervet International B.V., Boxmeer, the Netherlands) per 100 kg body weight subcutaneously, two of the severely affected cattle died (Table 1).
Table 1

Summary of clinical and laboratory data for cattle infected with piroplasms and Anaplasma marginale / A. ovis

Animal no.

Month when diseased (tested)

Age in year

Main clinical signs

Laboratory findings

Status/outcome

    

Haematocrit

Blood smear

Molecular

 

1.

November

3

lethargy, ataxia, oedema, recumbency1

27%

Bm

ne

death in 2 wk

2.

December

3

lethargy, oedema, recumbency

ne

Bm

ne

slaughtered

3.

December

10

haemoglobinuria, recumbency

ne

Bm

ne

death in 4 wk

4.

(January)

0.3

-

30%

ne

Bm

healthy

5.

(January)

0.5

-

normal

ne

Bm + Am

healthy

6.

(January)

8

anaemia

22%

ne

Tb

treated: recovery

7-9.

(January)

5,7,10

-

normal

ne

Tb + Am

healthy

1Clinical laboratory findings of animal No 1. [normal range]:

- elevated: aspartate-aminotransferase (AST): 337.2 [10–160] IU/l, alkaline phosphatase (AP): 310 [40–200] IU/l, glutamate dehydrogenase (GLDH): 116.8 [1025] U/l, carbamid: 6.6 [1–3.7] mmol/l, lactate dehydrogenase (LDH): 10050 [<450] U/l.

- lowered: albumin: 23.7 g/l [35–42].

Abbreviations: Bm Babesia major, Tb Theileria buffeli, Am Anaplasma marginale/A. ovis, ne not evaluated, wk week(s).

In January, PCR positivity to piroplasms was demonstrated in 7% of tested cattle (6 of 85, CI: 2.6-14.7%). Concerning these animals (No. 4–9: Table 1), haematocrit values were within the normal range in the case of four cattle co-infected with A. marginale/A. ovis (No. 5, 7–9) but were slightly lower in animal No. 4 harboring only B. major. In addition, cow No. 6 infected with T. buffeli (but not with B. major or A. marginale/A. ovis) was anaemic (Table 1). B. major and T. buffeli detected in this study had the highest (99%) sequence homology (with one nucleotide difference) to a French (GenBank: GU194290) and Spanish isolate (GenBank: DQ287959), respectively.

A. marginale/A. ovis msp4 PCR positivity was detected in 20 cattle (prevalence: 23.5%, CI: 15-34%), including four asymptomatic cows with Babesia/Theileria infection (Table 1). Sequencing was successful from two of the latter animals, revealing A. marginale with a sequence identity closest (95%) to A. ovis. A. phagocytophilum msp2 PCR positivity was shown in 11 animals (prevalence 13%, CI: 6.6-22%) that were all PCR-negative for piroplasms. Four cattle had concurrent A. phagocytophilum and A. marginale/A. ovis infection. During the study no clinical cases attributable to Anaplasma spp. were noted in the PCR-positive animals.

Discussion

Infection of the first animal in this study, which was grazing the same pastures for two years, may indicate that B. major has already been present in northeastern Hungary for some time. Alternatively, taking into account that B. major was hitherto not detected, it may have been recently introduced. In a region with endemic stability, the majority of locally born calves are exposed to babesiae while protected by maternal immunity and consequently develop long-lasting immunity to subsequent, homologous infection [1]. Therefore, clinical manifestations in the study herd, which was transferred to northeastern Hungary 1.5 months prior to the disease outbreak, may be explained by the absence of this innate resistance. Because the incubation period of bovine babesiosis is usually 1–3 weeks [1], all affected animals might have acquired their infection in northeastern Hungary. In support of this, the tick species H. punctata (which is the vector of both B. major and T. buffeli[23, 24] and is known to occur in Hungary [25]) was found in the pasture of affected animals during the present study (data not shown).

Bovine babesiosis caused by B. divergens was reported in Hungary from 1958 to 2005 and showed a steady decline [3]. In the country, I. ricinus (the vector of B. divergens) has its peak adult activity in May [25]. Accordingly, bovine babesiosis caused by B. divergens produced infections in the early summer [3]. On the contrary, in this study relevant clinical signs were noted in November, December and January. Thus, results of the present study might extend the seasonality of bovine babesiosis in Hungary, i.e., it can affect permanently pastured cattle during mild winters, which allow tick activity.

To the best of our knowledge, this is the first evidence regarding the occurrence of B. major and T. buffeli in central and eastern Europe. In southwestern Europe, where T. buffeli has been present for a long time, it usually has a high infection rate in cattle [9, 13]. This may reflect endemic stability in relevant countries [2] and would partly explain why this piroplasm does not appear to affect cattle [9, 13]. However, with the emergence of T. buffeli in formerly non-endemic countries, such as Hungary, even this mildly pathogenic species may be involved in pathologies, as shown here for the first time in central and eastern Europe. A similar phenomenon was observed during the emergence of T. buffeli in southern Italy between 1954 and 1995 [10] and more recently in the southern hemisphere for relevant genotypes of the T. orientalis complex [16].

Interestingly, a high proportion of animals were infected with A. phagocytophilum (also reported here for the first time in Hungarian cattle). However, during this study A. phagocytophilum was not detected in Babesia- or Theileria-infected animals, and therefore it most likely did not contribute to the anaemia of cattle affected by piroplasmosis.

The contribution of A. marginale/A. ovis to the clinical manifestations can also be discounted, because all three animals with lower haematocrit values were anaplasma-free. It was reported that a T. buffeli-carrier state in cattle may confer increased resistance to A. marginale infection, most likely involving non-specific, cell-mediated immunity [26]. Here, all three cattle that had concurrent Anaplasma and Theileria infections were healthy, unlike another animal, which was infected only with T. buffeli. Based on these results, further studies are warranted regarding whether the presence of these two pathogens is mutually protective.

Conclusions

The above findings extend the geographical range of B. major and T. buffeli in Europe. Although both piroplasm species are thought to cause mostly benign infections, in the present study B. major may have been responsible for the deaths of two cattle in the herd and T. buffeli for anaemia in another animal. A. phagocytophilum was detected for the first time in Hungarian cattle with relatively high prevalence (i.e., epidemiological importance) but no associated signs (i.e., low clinical importance).

Declarations

Acknowledgements

This study was partially funded by EU grant FP7-261504 EDENext and is catalogued by the EDENext Steering Committee as EDENext258. The contents of this article are the sole responsibility of the authors and do not reflect the views of the European Commission. The survey was organised in the framework of the EurNegVec COST Action TD1303.

Authors’ Affiliations

(1)
Department of Parasitology and Zoology, Faculty of Veterinary Science, Szent István University
(2)
Veterinary Clinic
(3)
SaBio. Instituto de Investigación en Recursos Cinegéticos IREC, CSIC-UCLM-JCCM
(4)
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University

References

  1. Bock R, Jackson L, de Vos A, Jorgensen W: Babesiosis of cattle. Parasitology. 2004, 129 (Suppl): S247-S269.View ArticlePubMedGoogle Scholar
  2. Bishop R, Musoke A, Morzaria S, Gardner M, Nene V: Theileria: intracellular protozoan parasites of wild and domestic ruminants transmitted by ixodid ticks. Parasitology. 2004, 129 (Suppl): S271-S283.View ArticlePubMedGoogle Scholar
  3. Hornok S, Edelhofer R, Szotáczky I, Hajtós I: Babesia divergens becoming extinct in cattle of Northeast Hungary: new data on the past and present situation. Acta Vet Hung. 2006, 54: 493-501. 10.1556/AVet.54.2006.4.7.View ArticlePubMedGoogle Scholar
  4. Zintl A, McGrath G, O'Grady L, Fanning J, Downing K, Roche D, Casey M, Gray JS: Changing epidemiology of the tick-borne bovine parasite. Babesia divergens. Parasit Vectors. 2014, 7 (Suppl 1): O8-10.1186/1756-3305-7-S1-O8.PubMed CentralView ArticleGoogle Scholar
  5. Hasle G, Bjune GA, Christensson D, Røed KH, Whist AC, Leinaas HP: Detection of Babesia divergens in southern Norway by using an immunofluorescence antibody test in cow sera. Acta Vet Scand. 2010, 52: 55-10.1186/1751-0147-52-55.PubMed CentralView ArticlePubMedGoogle Scholar
  6. Brocklesby DW, Sellwood SA, Harradine DL, Young ER: Babesia major in Britain: blood-induced infections in splenectomized and intact calves. Int J Parasitol. 1973, 3: 671-680. 10.1016/0020-7519(73)90093-3.View ArticlePubMedGoogle Scholar
  7. Purnell RE: Bovine babesiosis in the European community. Vet Sci Commun. 1978, 1: 289-296.View ArticleGoogle Scholar
  8. Malbert CH, Pangui LJ, Dorchies P, Ruckebusch Y: Babesia major: abomasal transmural potential difference, and antroduodenal motility changes associated with experimental infection in calf. Ann Rech Vet. 1988, 19: 237-243.PubMedGoogle Scholar
  9. García-Sanmartín J, Nagore D, García-Pérez AL, Juste RA, Hurtado A: Molecular diagnosis of Theileria and Babesia species infecting cattle in Northern Spain using reverse line blot macroarrays. BMC Vet Res. 2006, 2: 16-10.1186/1746-6148-2-16.PubMed CentralView ArticlePubMedGoogle Scholar
  10. Ceci L, Kirvar E, Carelli G, Brown D, Sasanelli M, Sparagano O: Evidence of Theileria buffeli infection in cattle in southern Italy. Vet Rec. 1997, 140: 581-583. 10.1136/vr.140.22.581.View ArticlePubMedGoogle Scholar
  11. Papadopoulos B: Cattle and small ruminant piroplasmosis in Macedonia, Greece. Parassitologia. 1999, 41 (Suppl 1): 81-84.PubMedGoogle Scholar
  12. Gubbels MJ, Hong Y, van der Weide M, Qi B, Nijman IJ, Guangyuan L, Jongejan F: Molecular characterisation of the Theileria buffeli/orientalis group. Int J Parasitol. 2000, 30: 943-952. 10.1016/S0020-7519(00)00074-6.View ArticlePubMedGoogle Scholar
  13. Gomes J, Soares R, Santos M, Santos-Gomes G, Botelho A, Amaro A, Inácio J: Detection of Theileria and Babesia infections amongst asymptomatic cattle in Portugal. Ticks Tick Borne Dis. 2013, 4: 148-151. 10.1016/j.ttbdis.2012.07.002.View ArticlePubMedGoogle Scholar
  14. EFSA Panel on Animal Health and Welfare: Scientific opinion on geographic distribution of tick-borne infections and their vectors in Europe and the other regions of the Mediterranean Basin. EFSA Journal. 2010, 8: 1723-Google Scholar
  15. Stockham SL, Kjemtrup AM, Conrad PA, Schmidt DA, Scott MA, Robinson TW, Tyler JW, Johnson GC, Carson CA, Cuddihee P: Theileriosis in a Missouri beef herd caused by Theileria buffeli: case report, herd investigation, ultrastructure, phylogenetic analysis, and experimental transmission. Vet Pathol. 2000, 37: 11-21. 10.1354/vp.37-1-11.View ArticlePubMedGoogle Scholar
  16. Kamau J, de Vos AJ, Playford M, Salim B, Kinyanjui P, Sugimoto C: Emergence of new types of Theileria orientalis in Australian cattle and possible cause of theileriosis outbreaks. Parasit Vectors. 2011, 4: 22-10.1186/1756-3305-4-22.PubMed CentralView ArticlePubMedGoogle Scholar
  17. Perera PK, Gasser RB, Firestone SM, Anderson GA, Malmo J, Davis G, Beggs DS, Jabbar A: Oriental theileriosis in dairy cows causes a significant milk production loss. Parasit Vectors. 2014, 7: 73-10.1186/1756-3305-7-73.PubMed CentralView ArticlePubMedGoogle Scholar
  18. Hornok S, Micsutka A, Fernández de Mera IG, Meli ML, Gönczi E, Tánczos B, Mangold AJ, Farkas R, Lutz H, Hofmann-Lehmann R, de la Fuente J: Fatal bovine anaplasmosis in a herd with new genotypes of Anaplasma marginale, A. ovis and concurrent haemoplasmosis. Res Vet Sci. 2012, 92: 30-35. 10.1016/j.rvsc.2010.10.011.View ArticlePubMedGoogle Scholar
  19. Pusterla N, Huder J, Wolfensberger C, Braun U, Lutz H: Laboratory findings in cows after experimental infection with Ehrlichia phagocytophila. Clin Diagn Lab Immunol. 1997, 4: 643-647.PubMed CentralPubMedGoogle Scholar
  20. Hornok S, Kováts D, Csörgő T, Meli ML, Gönczi E, Hadnagy Z, Takács N, Farkas R, Hofmann-Lehmann R: Birds as potential reservoirs of tick-borne pathogens: first evidence of bacteraemia with Rickettsia helvetica. Parasit Vectors. 2014, 7: 128-10.1186/1756-3305-7-128.PubMed CentralView ArticlePubMedGoogle Scholar
  21. Casati S, Sager H, Gern L, Piffaretti JC: Presence of potentially pathogenic Babesia sp. for human in Ixodes ricinus in Switzerland. Ann Agric Environ Med. 2006, 13: 65-70.PubMedGoogle Scholar
  22. de la Fuente J, Atkinson MW, Naranjo V, Fernández de Mera IG, Mangold AJ, Keating KA, Kocan KM: Sequence analysis of the msp4 gene of Anaplasma ovis strains. Vet Microbiol. 2007, 119: 375-381. 10.1016/j.vetmic.2006.09.011.View ArticlePubMedGoogle Scholar
  23. Yin H, Lu W, Luo J, Zhang Q, Lu W, Dou H: Experiments on the transmission of Babesia major and Babesia bigemina by Haemaphysalis punctata. Vet Parasitol. 1996, 67: 89-98. 10.1016/S0304-4017(96)01022-9.View ArticlePubMedGoogle Scholar
  24. Uilenberg G, Hashemi-Fesharki R: Theileria orientalis in Iran. Vet Q. 1984, 6: 1-4. 10.1080/01652176.1984.9693897.View ArticlePubMedGoogle Scholar
  25. Hornok S: Allochronic seasonal peak activities of Dermacentor and Haemaphysalis spp. under continental climate in Hungary. Vet Parasitol. 2009, 163: 366-369. 10.1016/j.vetpar.2009.03.048.View ArticlePubMedGoogle Scholar
  26. Gale KR, Leatch G, Dimmock CM, Gartside MG: Increased resistance to Anaplasma marginale infection in cattle chronically infected with Theileria buffeli (syn. T. orientalis). Vet Parasitol. 1997, 69: 187-196. 10.1016/S0304-4017(96)01125-9.View ArticlePubMedGoogle Scholar

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© Hornok et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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