- Open Access
Rhipicephalus (Boophilus) microplus aquaporin as an effective vaccine antigen to protect against cattle tick infestations
© Guerrero et al.; licensee BioMed Central Ltd. 2014
- Received: 11 July 2014
- Accepted: 3 October 2014
- Published: 12 October 2014
Vaccination as a control method against the cattle tick, Rhipicephalus (Boophilus) microplus has been practiced since the introduction of two products in the mid-1990s. There is a need for a vaccine that could provide effective control of R. microplus in a more consistent fashion than existing products. During our transcriptome studies of R. microplus, several gene coding regions were discovered to encode proteins with significant amino acid similarity to aquaporins.
A cDNA encoding an aquaporin from the cattle tick, Rhipicephalus microplus, was isolated from transcriptomic studies conducted on gut tissues dissected from fully engorged adult female R. microplus.
Bioinformatic analysis indicates this aquaporin, designated RmAQP1, shows greatest amino acid similarity to the human aquaporin 7 family. Members of this family of water-conducting channels can also facilitate the transport of glycerol in addition to water. The efficacy of this aquaporin as an antigen against the cattle tick was explored in cattle vaccine trials conducted in Brazil. A cDNA encoding a significant portion of RmAQP1 was expressed as a recombinant protein in Pichia pastoris, purified under native conditions using a polyhistidine C-terminus tag and nickel affinity chromatography, emulsified with Montanide adjuvant, and cattle vaccinated intramuscularly. The recombinant protein provided 75% and 68% efficacy in two cattle pen trials conducted in Campo Grande, Brazil on groups of 6 one year old Holstein calves.
The effectiveness of this vaccine in reducing the numbers of adult female ticks shows this aquaporin antigen holds promise as an active ingredient in cattle vaccines targeted against infestations of R. microplus.
- Cattle tick
- Recombinant protein
- Vaccine antigen
The cattle tick, Rhipicephalus (Boophilus) microplus, is an obligate parasitic cattle pest that has established populations throughout the world’s tropical and subtropical regions. R. microplus is responsible for significant economic losses to cattle producers because of direct effects through blood loss and damage to hides and indirect effects through diseases it transmits such as babesiosis and anaplasmosis. For example, Grisi et al.  estimated Brazil’s annual losses to R. microplus parasitism approximate US$3.2 billion. Significant efforts to control this tick are undertaken in most cattle-raising countries and these efforts presently center around the use of acaricides. However, acaricide resistant populations of R. microplus have become a major problem in most of the cattle-producing countries of the world and novel cattle tick control technologies are needed to maintain efficiencies in cattle production -.
Vaccination as a tick control method has been practiced since the introduction of two products in the mid-1990s, TickGARD  and Gavac©, that were developed using the midgut glycoprotein Bm86 as the immunoreactive antigen. TickGARD is no longer commercially available, but Gavac© continues to be used to date, primarily in North and South America. Although the results with Gavac© have been mixed, within integrated tick management systems in some geographic regions, the vaccine has proven to reduce the number of acaricidal applications per year that are required to control R. microplus at acceptable levels . As an interesting sidelight to the role of Gavac© in cattle tick control, the product has been shown to provide >99% efficacy against Rhipicephalus annulatus, a second cattle tick species which is much less prevalent and invasive than R. microplus,. Nevertheless, the need remains for a vaccine that could provide effective control of R. microplus in a more consistent fashion than Gavac©. As part of research mining the genome of R. microplus for transcripts that would produce effective antigens for a cattle tick vaccine, focused genome , transcriptome , and proteome , studies in R. microplus have led to identification of genes and gene coding regions that encode proteins with critical functions in the tick ,).
Several of these gene coding regions were discovered to encode proteins with significant amino acid similarity to aquaporins. Aquaporins, originally called water channels, allow the regulation of water transport across the highly hydrophobic lipid bilayer of cell membranes. Members of the aquaporin family have been found in animal taxa from mammals  to bacteria  and they are very common in certain cell types, with approximately 150,000 protein copies per red blood cell . The structure of the aquaporins is such that two constrictions in the protein act as filters whose selectivity for water, glycerol, urea, and other small molecules is determined by the size and charge of the constriction pore . Because cattle ticks ingest large volumes of blood relative to their body size and weight, they are required to have efficient water transport mechanisms so as to concentrate the blood components for efficient digestion . Thus, the tick aquaporins are critical to tick physiology and appeared a good protein to target as an anti-cattle tick vaccine candidate. The full length transcript for one of the discovered aquaporins, designated RmAQP1, was determined and a fragment of the open reading frame (ORF) was expressed and purified as a recombinant protein in Pichia pastoris. This recombinant protein was tested in cattle pen trials for efficacy as a vaccine antigen against R. microplus.
Source of tick materials
Ticks that were the source of DNA and RNA for the transcript discovery study were obtained from engorged adult female R. microplus of the f20 La Minita strain maintained at The University of Idaho Holm Research Center (Moscow, ID). The La Minita strain was originally collected in 1996 during an outbreak in Starr County, TX and propagated at the USDA-ARS Cattle Fever Tick Research Laboratory in Edinburg, TX. Tissues that were the source of RNA for gene expression study were dissected from 25 1-2 day old adult male and female ticks from the R. microplus Deutch strain f41 generation maintained at the Cattle Fever Tick Research Laboratory. The Deutch strain is an organophosphate and pyrethroid susceptible strain originating from an outbreak in 2001 in Webb County, Texas. Tick larvae used in this study to infest cattle for the cattle vaccine trials were obtained from a laboratory colony maintained at EMBRAPA Beef Cattle. The colony was established with R. microplus ticks collected from infested cattle in Campo Grande, MS, Brazil. Larvae used for infesting cattle in the vaccine trials were 18 days post-hatch. During the vaccine trials, fully engorged adult female ticks were collected upon host detachment and brought to the laboratory to allow oviposition. Egg masses were incubated in humidity chambers at 28°C and 95% relative humidity to facilitate hatching. Larvae were used for infestation at 18 days after hatching.
RNA purification, cDNA synthesis & RACE
Total RNA was isolated using the FastPrep-24 Tissue and Cell Homogenizer and Lysing Matrix D (Qbiogene, Irvine, CA, USA) as described in Saldivar et al.  from gut tissue dissected from 5 engorged adult female R. microplus from the La Minita strain. The total RNA was DNAse treated following manufacturer’s protocol using Turbo DNA-free kit (Ambion, Austin, TX, USA).
Primers/Probes used for RmAQP1 transcript cloning and real-time PCR
5′ GAGCGGGCACATGCAGTTGTAGGC 3′
Reverse for Aquaporin 5′ RACE
5′ ACTCAGGAATTC ATGAAGATCGAGAACCT 3′
Forward for insertion into pPICZ αA EcoRI
5′ TCACTGGCGGCCGC CGGGCACATGCAGTTGTAGGC 3′
Reverse for insertion into pPICZ αA NotI
5′ TCGCCAAAGTGCCGCTATAC 3′
Aquaporin RT-PCR Forward
5′ CGTCTTTGTAGGTGGCAAACAC 3′
Aquaporin RT-PCR Reverse
5′ 6FAM-CGCCGCACCGACGAAGCCAC-TAMRA 3′
Aquaporin TaqMan Probe
5′ TAAGGACCTGTACGCCAACAC 3′
Beta-actin RT-PCR Forward
5′ CGGTGATTTCCTTCTGCATACG 3′
Beta-actin RT-PCR Reverse
5′ 6FAM-TCTCCGGCGGCACCACCATGTACC-TAMRA 3′
Beta-actin TaqMan Probe
5′ CCTGAGAAACGGCTACCACATC 3′
18S rRNA RT-PCR Forward
5′ GTGCCGGGAGTGGGTAATT 3′
18S rRNA RT-PCR Reverse
5′ 6FAM-AGGAAGGCAGCAGGCGCGC-TAMRA 3′
18S rRNA TaqMan Probe
Real-time PCR gene expression study
Quantitative PCR studies were designed with the MIQE guidelines in mind . Tissue dissections were performed under phosphate-buffered saline pH =7.4. The tissues dissected from the female ticks were the synganglia, salivary glands, ovaries and midgut while tissues dissected from the male ticks were the synganglia, salivary glands, testes, accessory gland and midgut. Dissected tissues were placed in RNALater (Ambion, Austin, TX, USA) according to manufacturer’s protocol. Total RNA was isolated using the ToTALLY RNA Kit (Ambion) and DNase treated using the Turbo-DNA free kit (Ambion) according to manufacturer’s protocol. The RETROscript Kit Reverse Transcription for RT-PCR (Ambion) was used to produce cDNA from each tissue using 0.1 μg of DNase-free total RNA.
TaqMan probes and primers were designed using Beacon Designer 7.0 (PREMIER BioSoft International, Palo Alto CA; Table 1) and synthesized by Sigma-Aldrich Inc. (The Woodlands, TX, USA) for RmAQP1 and the two reference genes used for normalization, R. microplus 18S rRNA gene  and beta-actin. Optimization PCRs were run on all three genes to determine optimal reaction conditions, PCR efficiencies, and optimal reagent concentrations. Real-time PCR reactions were carried out in clear low profile 96 well plates (BioRad, Hercules, CA, USA) with microseal film B (BioRad) using 25 μL total volume reactions, which included TaqMan Universal Master Mix No AmpErase UNG (Applied Biosystems Inc.), 250 nM TaqMan probe, tissue specific RETROscript cDNA, and 900 nM forward and reverse primers for all three genes. The cycling profile used on the BioRad CFX96 Real-Time System was 95°C for 10 min, and 50 cycles of 95°C for 15 sec, 60°C for 1 min plus plate read. All samples were run in triplicate and both no-template and no-reverse transcriptase controls were utilized to verify DNA-free status of the samples. The fluorescence emission data analysis for the relative standard curve method for quantification was done using baseline subtracted curve fit mode with CFX Manager Software v1.5 (BioRad).
Cloning into Pichia pastoris
The partial RmAQP1 ORF used for the vaccine study was amplified with the Advantage® 2 PCR Enzyme System (Clontech Laboratories Inc.) using primers KB-156 and KB-157 (Table 1). The 597 bp amplification product was purified and gel extracted as described above. The RmAQP1 DNA was prepared for ligations by restriction enzyme digestion reactions with EcoRI and NotI (Life Technologies) per manufacturer’s protocol.
The EasySelect Pichia Expression Vector (Life Technologies), pPICZ αA restriction enzyme-digested with EcoRI and NotI and purified, was ligated onto the RmAQP1 DNA using the TA Cloning Kit (Life Technologies) using the TA Cloning Kit protocol and 137 ng RmAQP1 insert, 50 ng pPICZ αA EcoRI/NotI digested vector, and 1 unit T4 DNA ligase incubated for 17 hr at 4°C. OneShot TOP10 Electrocomp cells (Life Technologies) were transformed with ligation reaction and plated on low salt LB agar (1% tryptone, 0.5% yeast extract, 0.5% sodium chloride, 1.5% agar) with 25 μg/mL ZeocinTM (Life Technologies). Resulting colonies were screened via PCR using vector primers 5′AOX1 and 3′ AOX1 and DNA isolated from positive colonies using the QIAprep Spin Miniprep Kit (Qiagen) according to manufacturer’s instructions. The sequence of both strands of putative positive clone plasmid DNA was verified by DNA sequencing, followed by analysis with MacVector with Assembler version 10.0.2.
According to the EasySelect Pichia Expression Kit protocol, a freshly prepared 80 μL aliquot of electrocompetent P. pastoris KM71H strain and 5 μg recombinant expression vector DNA linearized with SstI was used for transformations according to the manufacturer’s instructions using the Bio-Rad Gene Pulser and Pulse Controller at pulse settings of 1.5 kV, 200′ and 25μFD. Transformation mixtures were plated on YPDS (1% yeast extract, 2% peptone, 2% dextrose, 1 M sorbitol, 2% agar) plates containing 100 μg/mL ZeocinTM and incubated at 30°C for four days to allow colonies to develop.
Analysis of Pichia pastoris transformants
Direct screening of individual Pichia KM71H colonies using PCR was done by modifying the direct screening protocol from Linder et al.  and the EasySelect Pichia Expression Kit manual with 25 μL reactions using the 5′ and 3′ AOX1 vector primers and 0.16 μL of a 1 vol:1 vol mix of AmpliTaq DNA polymerase (5 U/μL stock; Applied Biosystems) and TaqStart antibody (1.1 μg /μl stock; Clontech). Colonies containing the expected 1,192 bp recombinant product were re-screened using a similar approach but substituting RmAQP1-specific primers.
Selected colonies were Mut phenotyped and small-scale expression experiments used to determine the optimal method and conditions for the expression of the recombinant proteins. These protocols are described in the EasySelect Pichia Expression Kit manual for 3 mL cultures grown in BMGY (1% yeast extract, 2% peptone, 100 mM potassium phosphate pH =6.0, 1.34% Yeast nitrogen base with ammonium sulfate without amino acids, 4 × 10-5% biotin, 1% glycerol) and BMMY media (BMGY but substituting 0.5% methanol for the 1% glycerol). BMMY cultures were replenished to 0.5% final methanol concentration every 24 hr. Samples were collected at various time points and centrifuged to separate the yeast cells from the culture media supernatant.
Supernatants were frozen in liquid nitrogen and stored at -80°C. Intracellular proteins were purified by a protocol similar to that described in the EasySelect Pichia Expression Kit manual. Briefly, 100 μL of breaking buffer (50 mM sodium phosphate pH7.4, 1 mM EDTA, 5% glycerol) +1X FOCUS ProteaseArrest (GBioscience, St. Louis, MO) was used per cell pellet from a 1 ml culture sample. An equal volume of 0.5 mM acid-washed glass beads was added and the sample vortexed for 30 sec and set on ice for 30 sec. A total of 8 vortex/ice cycles were used, the sample frozen at -80°C, thawed and 8 more vortex/ice cycles used before a final short centrifugation to clarify the sample. Samples were concentrated in Amicon Ultracel units (Millipore, Billerica, MA) when necessary.
Both the intracellular cell pellets and the secreted supernatant samples were analyzed by denaturing gel electrophoresis under reducing conditions using the NuPAGE® Electrophoresis System and NuPAGE® 4-12% Bis-Tris gels in the XCell SureLockTM Mini-Cell with 1X NuPAGE MOPS SDS Running Buffer (Life Technologies) according to manufacturer’s instructions. Proteins were visualized by staining with Coomassie Brilliant Blue R-250 using a modified Fairbank’s method . Recombinant aquaporin was localized in the cell pellet sample with maximal expression seen after 4 days of induction growth in BMMY.
After the optimal clone and growth conditions were determined, a large scale culture of the clone producing the highest amount of recombinant aquaporin protein was grown in 25 ml BMGY media in 500 ml baffled flasks in a shaking incubator at 30°C to an OD600 = 2-6. Cells were harvested by centrifugation and resuspended in BMMY to an OD600 = 1 and returned to the incubator for 4 days to induce expression. Every 24 hr, methanol was added to a final concentration of 0.5% to maintain induction and cells were harvested 4 days post-induction. Following centrifugation, the cell pellet was frozen at -70°C until protein extraction.
Total yeast intracellular protein was extracted similarly as described above for the small-scale expression cell pellet protocol with the exception of using 50 ml Breaking Buffer with 1X Protease Arrest and 10 cycles of 30 sec vortexing followed by 30 sec on ice. The cell pellet lysates were then frozen at -70°C overnight and thawed followed by 10 vortex/ice cycles. The protein solution was clarified by centrifugation and the resulting solution concentrated using Centricon Plus-70 Centrifugal Filter Devices (Millipore).
Purification of expressed recombinant protein
Recombinant protein was purified making use of the 6X-Histidine tag supplied by the vector sequence and the ProBond Purification System (Life Technologies) using ProBondTM nickel-chelating resin under native conditions, initially according to manufacturer’s instructions. We wished to preserve the native protein structure, thus we did not use urea, SDS, or heat in the purification steps. However, the purified protein presented solubility problems upon freezing and thawing and we adapted the ProBond purification steps to utilize buffer (50 mM NaH2PO4 pH = 8.0, 300 mM NaCl, 2 mM β-mercaptoethanol, 0.4% β -D-1-thioglucopyranoside) plus 10 mM imidazole for binding, the same buffer plus 30 mM imidazole for washing, and buffer plus 300 mM imidazole for elution . Eluted protein was concentrated using Amicon Ultra-15 centrifugation units (Millipore) and, following concentration, the solution was made 50% v/v glycerol and stored at -20°C. This protein solution was used to prepare the vaccine. Protein concentration was quantified by the BioRad Protein Assay Kit I with bovine plasma gamma globulin protein standards, and purity of the protein solution verified by gel electrophoresis as described above using the NuPAGE® Electrophoresis System and NuPAGE® 4-12% Bis-Tris gels in the XCell SureLockTM Mini-Cell.
Protein identity was verified by mass spectrometry analysis and Western blotting, taking advantage of the c-myc and 6X-His tag epitopes on the recombinant protein that are provided by the expression vector sequence. The WesternBreeze Chromogenic Kit and Anti-myc-HRP and Anti-His(C-term)-HRP antibodies (Life Technologies) were utilized with standard protocols provided by the supplier. The supplier-provided alkaline phosphatase-conjugated secondary antibody was utilized to enhance sensitivity. The mass spectrometry analysis was done by Protea Bioscience Group (Morgantown, WV). The recombinant protein (in 50% glycerol solution described above) was purified by 1-D acrylamide gel electrophoresis, extracted from the gel matrix, and digested with trypsin. The resulting peptides were analyzed by LC-MS/MS using an ABSciex5500 Series QTRAP for tandem MS data acquisition followed by a search for peptide matches to the expected sequence of purified antigen.
Controlled pen trials were conducted to evaluate the immunogenic and protective capacity of the aquaporin antigen adjuvated with Montanide ISA 61 VG (Seppic, Paris) into doses of 2 ml containing 100 μg of the recombinant protein and phosphate-buffered saline (PBS). One-year-old Holstein calves were randomly distributed into groups of six animals each. Negative controls were injected with adjuvant prepared with PBS alone. The animals were injected intramuscularly three times with two week intervals between injections. Serum samples were taken from each animal before the first immunization and weekly thereafter. Twenty-one days after the last injection the animals were challenged with 15,000 larvae of the Campo Grande tick strain. These larvae were delivered in three applications of 5,000 larvae each during one week, placed in separate vials onto the back of the animals. As engorged female ticks detached, they were collected once a day, pooled, and weighed. This sampling was initiated upon the first day detachment started and continued until tick detachment ceased, which was 19 days for Trial 1 and 16 days for Trial 2. Twenty females from each day’s collection were pooled and incubated at 29°C and 85% humidity until egg laying was complete. Eggs collected from each pool were weighed and incubated at 29°C and 85% humidity until hatching was completed to determine the hatch percentage for each pool.
Bovine serum collection and analysis
Bovine blood was sampled weekly and separated serum frozen until analyzed by ELISA. For the ELISA, sera from all animals in each group were pooled according to day of collection. Microtiter plates were coated with antigen (50 μL per well of a 1 μg antigen/ml solution in 20 mM sodium carbonate buffer, pH 9.6) and incubated overnight at 4°C. Blocking with 2% bovine serum albumin in PBS pH 7.4 containing 0.05% Tween 20 was followed by washing five times with PBS pH 7.4. The plates were incubated for 45 min at 37°C with 100 μL per well of immunized bovine serum diluted to 1:100 in PBS. After washing in PBS pH 7.4, 50 μL of rabbit anti-bovine IgG peroxidase conjugate (Sigma, St. Louis, MO, USA) diluted 1:20,000 was added and the plate incubated for 30 min at room temperature. After washing in PBS pH 7.4, 50 μL of 1.0 mM chromogenic substrate o-phenylenediamine was added and the reaction was stopped after 15 min by adding 50 μL of 0.2 M NaOH. A microplate reader was used to assess the results with absorbance set at 490 nm.
Efficacy assessment and statistics
Reductions associated with immunization relative to the unvaccinated group were determined for numbers of adult female ticks, egg production, and larval hatching. Vaccine efficacy was calculated as 100 × [1 - (NET × EWPF × H)], where NET, EWPF, and H represent the fraction of the relevant tally in the immunized group relative to that in the control group of the total number of adult female ticks, total weight of eggs per female, and % hatch of eggs, respectively.
The La Minita ticks used for the transcript discovery study were reared at The University of Idaho Holm Research Center (Moscow, ID, USA) following protocols approved by the University of Idaho Institutional Animal Care and Use Committee (IACUC). The Deutch ticks used for the gene expression studies were reared at the USDA- ARS Cattle Fever Tick Research Laboratory (Edinburg, TX, USA) with protocols approved by that Laboratory’s IACUC. The cattle vaccine studies were conducted at EMBRAPA Beef Cattle (Campo Grande, MS, Brazil) under protocols approved by the EMBRAPA review board.
Aquaporin-like sequences from the cattle tick
Gene expression of RmAQP1 in various tissues of R. microplus
Relative expression level
Normalized to 18S
Normalized to β-actin
Adult male ticks
Adult female ticks
Production of recombinant aquaporin as vaccine antigen
Cattle pen tests for aquaporin antigen efficacy against R. microplus
Data from cattle stall trials evaluating RmAQP1-derived protein for efficacy as anti- R. microplus vaccine antigen
Overall tick yield
Tests with eggs
Total Wt (g)
Tick Wt (g)
Egg Wt (g)
The efficacy of the aquaporin-derived antigen vaccine against R. microplus in our tests was substantial enough to warrant further investigation as a potential control technology against this parasite. Prior to the two pen trials described here, we had conducted a cattle pen trial using a DNA vaccine approach and an expression vector encoding the aquaporin-derived antigen described herein . We obtained approximately 50% efficacy against R. microplus (data not shown) while a rBmiTI antigen had approximately 30% efficacy as reported by Andreotti et al. . Additionally, during the vaccination trials reported here, we also had other antigens being evaluated for efficacy against R. microplus. For example, in Trial 1 a salivary gland antigen and in Trial 2 a Bm86-Campo Grande antigen was evaluated as a separate group in the pen tests and showed 28% and 49% efficacy, respectively (data not shown). Thus, the aquaporin-derived vaccine was shown to outperform the other vaccines in both our pen trials. The vaccine’s major impact on R. microplus was to drastically reduce the yield of adult ticks (Table 3). Effects on average detached female tick weight, average egg mass weight, and hatch were absent or minor (Additional file 2: Table S2).
An aquaporin from I. ricinus, IrAQP1 (EMBL Accession Number FN178519), was evaluated for efficacy using in vivo feeding assays following dsRNA interference . In contrast to our results, the effects from the IrAQP1 tests were manifested in significant weight reduction in treated ticks, due to reduced blood ingestion. However, reductions in adult tick mortality were not seen. There are a number of reasons that might explain the differences between these aquaporin efficacy tests. There are extensive differences between these two tick species. For example, I. ricinus is a three-host tick with an extended life cycle while R. microplus parasitizes a single host with a relatively fast life cycle. Also, IrAQP1 and RmAQP1 could be members of different aquaporin families as they have different expression patterns in different tick tissues. RmAQP1 was expressed most highly in the synganglia of both males and females (Table 2), while IrAQP1 was not detected in male adult I. ricinus or the synganglia of adult female I. ricinus. Ball et al.  characterized the RsAQP1 from R. sanguineus and found highly similar amino acid sequence and a similar tissue expression pattern as IrAQP1. They described the tick aquaporins as falling into two families based on phylogenetic analysis of the existing aquaporin sequences at the time. Our phylogenetic analysis (Figure 3) maintains the relationships between the aquaporins of R. appendiculatus, R. sanguineus, I. ricinus, and D. variabilis noted by Ball et al.  with two families of aquaporins noted. However, in our phylogenetic analysis the inclusion of the additional 3 aquaporins from R. microplus discovered in our studies and our use of a different I. scapularis aquaporin appears to break out an additional aquaporin family that includes RmAQP1. This is consistent with our tissue expression results, as RmAQP1 is the first reported tick aquaporin that has substantial expression in synganglion tissue. We attempted to determine a classification of the RmAQP1-3 in conjunction with the human aquaporin classifications to perhaps learn more about the aquaporins from R. microplus. Using PSI-BLAST with 4 iterations against the NCBI nr protein database entries for Homo sapiens, all 3 R. microplus aquaporins had highest sequence similarity to several HsAQP7-like transcripts, an aquaglyceroporin (data not shown). However, there was also significant similarity to other aquaporin families, including HsAQP3, HsAQP10, HsAQP9, HsAQP4. Thus, this approach did not shed much light upon the transport capabilities of the aquaporins of R. microplus.
We have identified 3 aquaporin-like full length ORFs from R. microplus transcriptome datasets and a large part of one of those aquaporins, RmAQP1, was discovered to be an efficacious vaccine antigen in Brazilian Holstein calves infested with larvae from the Campo Grande strain of R. microplus. Further work is underway to evaluate the general effectiveness of this vaccine in different breeds of cattle and different geographical locations.
FDG conducted the transcriptomic experiments that discovered the AQPs, designed the vaccine antigen and expression strategies, and led the drafting of the manuscript. RA led the design and implementation of the cattle pen tests and helped draft and revise the manuscript. KGB conducted the various PCR studies, produced the recombinant antigen, and helped draft and revise the manuscript. RCC conducted the cattle pen tests and the ELISA study. RJM and KY performed the statistical analysis of the cattle pen test data and revised the manuscript. AAPL helped design the cattle pen tests and revise the manuscript. All authors approved the final version of the manuscript submitted for publication.
Embrapa Beef Cattle, CNPq, and Fundect are gratefully acknowledged for financial support. This article reports the results of research only. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation of endorsement by the U.S. Department of Agriculture. F.D. Guerrero, K.G. Bendele and A.A. Pérez de León are funded by USDA-ARS appropriated project 6205-32000-031-00D. The U.S. Department of Agriculture is an equal opportunity provider and employer.
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