Not “out of Nantucket”: Babesia microti in southern New England comprises at least two major populations
© Goethert and Telford; licensee BioMed Central. 2014
Received: 17 October 2014
Accepted: 18 November 2014
Published: 10 December 2014
Deer tick-transmitted human babesiosis due to Babesia microti appears to be expanding its distribution and prevalence in the northeastern United States. One hypothesis for this emergence is the introduction of parasites into new sites from areas of long-standing transmission, such as Nantucket Island, Massachusetts.
We developed a typing system based on variable number tandem repeat loci that distinguished individual B. microti genotypes. We thereby analyzed the population structure of parasites from 11 sites, representing long-standing and newly emerging transmission in southern New England (northeastern United States), and compared their haplotypes and allele frequencies to determine the most probable number of B. microti populations represented by our enzootic collections. We expected to find evidence for a point source introduction across southern New England, with all parasites clearly derived from Nantucket, the site with the most intense longstanding transmission.
B. microti in southern New England comprises at least two major populations, arguing against a single source. The Nantucket group comprises Martha's Vineyard, Nantucket and nearby Cape Cod. The Connecticut/Rhode Island (CT/RI) group consists of all the samples from those states along with samples from emerging sites in Massachusetts.
The expansion of B. microti in the southern New England mainland is not due to parasites from the nearby terminal moraine islands (Nantucket group), but rather from the CT/RI group. The development of new B. microti foci is likely due to a mix of local intensification of transmission within relict foci across southern New England as well as long distance introduction events.
KeywordsBabesia microti New England Epidemiology VNTR Emergence
Babesiosis due to Babesia microti (Apicomplexa:Sporozoea) was first recognized as a zoonosis in 1969 when a resident of Nantucket Island, Massachusetts, sustained a malaria-like illness. Nearly a dozen cases were identified from Nantucket by 1976, leading to its colloquial name at the time, “Nantucket fever”. The white-footed mouse, Peromyscus leucopus, was incriminated as a main reservoir, with deer ticks (Ixodes dammini; also known as the northern population of Ixodes scapularis) as the vector ,. Classic epidemiological and ecological studies by Spielman and Piesman described the enzootic cycle of B. microti on Nantucket and elsewhere in coastal New England, subsequently providing the basis for our rapid understanding of that of the spirochetal agent of Lyme disease (Borrelia burgdorferi), once I. dammini was incriminated as the vector of that bacterial zoonosis. Other animals (shrews, voles, chipmunks, squirrels) have been found infected by B. microti but their reservoir capacity remains largely undescribed ,. The potential for enzootic transmission of B. microti would seem to rest mainly on the presence of a suitable Ixodes sp. vector and small rodents or insectivores.
For the first two decades after the index case of Nantucket fever, babesiosis was considered a rare infection that affected residents of the terminal moraine sites of New England and New York (Nantucket, Martha’s Vineyard, Cape Cod, Block Island, eastern Long Island), as well as a focus in the upper Midwest . In addition, babesiosis was thought to affect only those people who were elderly, immune compromised or asplenic, thereby limiting its perceived public health significance despite the fact that those individuals sustained severe complications with a case fatality rate of 6- 9% . In young, healthy individuals, infection with B. microti is usually asymptomatic and treatment may not be indicated. However, asymptomatic infection in healthy individuals threatens our blood supply, and more than 160 babesiosis cases have been acquired by transfusion. Many transfusion recipients are immunocompromised or have comorbidities and thus severe disease may result; about 20% of these cases are fatal. Babesiosis is now considered the greatest threat to our blood supply due to an infectious agent . Accordingly, our perception of the clinical significance of babesiosis has changed over the last two decades.
Within the last decade, the northeastern U.S. has experienced a rising incidence of disease, although there is some confounding due to the fact that few states mandated reporting, and babesiosis only became nationally reportable in 2011. There has been a marked expansion of the geographic range of human disease. Cases have now been diagnosed in all New England states, north into Maine, west into the upper Hudson River valley, eastern Pennsylvania and New Jersey and as far south as Maryland -. This expansion has not been limited to the northeastern U.S. The Midwestern foci in Minnesota and Wisconsin has also intensified in transmission and expanded in distribution ,. The biological basis for the expanded distribution remains undescribed. The public health burden of Lyme disease has changed significantly within the last 30 years, generally as a result of the expanded range and increased density of the tick vector, a function of dispersal by birds, habitat fragmentation, and burgeoning deer herds. However, the dramatic change in Lyme disease risk over time was not accompanied by that of babesiosis, at least until the last decade. For example, Westchester County, perhaps the epicenter of Lyme disease risk since the late 1980s, did not identify babesiosis in residents there until 2001 . This lag in risk relative to Lyme disease was thought to be consistent with the mode of dispersal of the two infections, because B. burgdorferi could be transported by reservoir competent birds and subadult I. dammini infesting them; B. microti would inefficiently be introduced to new sites because birds are likely not reservoirs for this protozoan and transported nymphs would not seek competent hosts as adult ticks. The empiric evidence for a recently expanded geographic distribution of zoonotic B. microti is a paradox given these considerations. To determine whether the expanded distribution of zoonotic B. microti reflects the introduction of parasites from longstanding sites of transmission into new sites, we compared the genetic diversity of parasites from Nantucket and Martha’s Vineyard with that of those from mainland New England sites, including more recently established sites of transmission. In particular, we developed variable number tandem repeat (VNTR) markers based on the published B. microti genome , and used the resulting allele frequencies to determine the most probable number of B. microti populations represented by our enzootic collections. Limited genetic diversity across our sampling sites would provide evidence for a “transport-introduction” hypothesis to explain the recently expanded distribution of B. microti babesiosis.
Sources of B. microti DNA
Archived collections of tick or small mammal blood samples were mainly from long-standing field studies of I. dammini population biology ,, in coastal New England. Martha's Vineyard and Nantucket Island were the sites first experiencing zoonotic babesiosis . B. microti was documented in rodents from Ipswich (Essex County), Sandy Neck and Great Island (Cape Cod, Barnstable County), Prudence Island and Block Island, Rhode Island; and coastal Connecticut by the 1980s -. In contrast, Dover (Norfolk County) and Grafton (Worcester County) in central Massachusetts are newly emergent transmission sites: the first cases of likely authochthonous babesiosis occurred in Norfolk County in the mid 2000s and, to date, there have been none documented in Grafton, even though there has been low level enzootic transmission there since at least 2002 (unpublished). Rodent blood was stored at -20C or dried on filter paper. Drag sampled host seeking nymphal deer ticks were stored desiccated, in 70% ethanol, or frozen until analysis; extracted DNA was also archived. DNA was extracted from blood using a DNEasy kit (Qiagen). Ticks were macerated individually and then extracted using a HOTSHOT protocol . Extracted DNA was screened for the presence of B. microti DNA by PCR using the BmITS1F/BmITS1R primer set .
Identification and evaluation of tandem repeat markers
The B. microti genome (GenBank FO082871, FO08272 and FO082874) was searched for tandem repeats using the Tandem Repeats Finder (http://tandem.bu.edu/trf/trf.basic.submit.html). Primers targeting the flanking sequence were designed using Primer3 . Amplification was done using Picomaxx (Agilent Technologies) high fidelity taq polymerase using a step-down cycling protocol; the concentration of each primer was 0.5 micromolar in each 15 uL reaction. Template concentration was not measured; 1.5 uL was added directly from the HOTSHOT extract. Amplification cycles were started at 65C, decreasing by 1 degree each cycle until 55C then 55 cycles were run at the 55C annealing temperature. The forward primers were fluorescently labeled using either FAM or HEX (Integrated DNA Technologies). Amplicons were then mixed with either the GeneScan500 (Applied Biosystems) or MapMarker1000 (BioVentures) ladders, depending on the expected size range, and sent to University of Maine Sequencing Facility at Orono, ME for accurate sizing on a capillary-based sequencer. The resulting data was analyzed by hand using PeakScanner (Applied Biosystems) and STRand software .
The amplicon from each potential tandem repeat locus was assessed for size differences between laboratory B. microti strains originating from Spooner, Wisconsin and Nantucket Island, Massachusetts, as well as the expected size from the genome posted on GenBank. If the marker was invariant among these samples, it was rejected. Markers were also rejected for failure to amplify well or for amplification of multiple bands. The final 9 loci were multiplexed for amplification as follows: BMV1 and 2, BMV 4 and 5, BMV 8 and 10, and BMV 13 and 23. BMV 20 was amplified alone. Samples that did not amplify well with the multiplex assays were repeated with each locus individually before being deemed a failure. The ability to detect multiple peaks was evaluated by mixing DNA from 2 samples with different genotypes together at varying concentrations. Minor peaks were counted if they were greater than 40% of the major peak. Samples that had more than 2 loci that failed to amplify to were excluded from further analysis. Babesia microti is haploid in the mammalian host but has diploid stages in its tick host . As with recent malarial population structure studies (- among others), we simplified the genetic analysis by assuming that parasites are haploid. Samples that had multiple peaks were assumed to be due to infection with two separate parasites and were treated as such. Twenty five samples had 2 peaks in more than one locus. These were excluded from the analysis because we were unable to definitively identify the genotype of each haploid.
The fidelity of the markers was evaluated in 2 ways. In the laboratory, the well characterized GI (Harvard) strain  has been continuously cycled from hamster to subadult deer tick to hamster since its isolation from a Nantucket human case in 1981. Parasites at each stage (bloodstream infection, engorged larvae, molted infected nymphs) were typed to determine whether scoring the markers was sensitive to changes in ploidy over the transmission cycle. To determine the long term stability of the markers in the field, field samples (host-seeking ticks or rodent blood) collected on Nantucket Island from 1986 to 2013 were compared. Diversity indices and diversity permutation tests were calculated using PAST  and expressed with the 95% confidence interval around the index.
Identification of population clusters
The most probable number of populations (K) was estimated using the program Structure . Structure uses a Bayesian approach in which it assigns individuals probabilistically to a predefined number of populations based on allele frequencies and without prior knowledge of geographic sampling. It is not straight forward to determine the optimal number of populations (K) for a given data set. We therefore calculated delta K, which is the second order rate of change in the likelihood of K. Simulation studies have shown that the modal value of this distribution represents the uppermost level of structure or the "true" K of a given set of data . The height of the modal value indicates the strength of the signal detected by Structure. The probable evolutionary relationships (lineages) among our samples were analyzed using Phyloviz . This program uses the eBURST algorithm to attempt to identify groups of related organisms and their founding genotype. It then creates a radial diagram predicting the patterns of descent to the other genotypes in the group.
In all years, mammals were collected under permits issued by the respective state divisions of fish and wildlife. Prior to 2003, when we were at Harvard University, mammalogy fieldwork did not require institutional animal care and use committee approval; all fieldwork since 2003 was conducted under research protocols approved by the Tufts University IACUC.
The VNTR loci used in the study and the observed (or predicted for the genome) amplicon sizes from the initial screen for variability
Primers (5' to 3')
Observed size (bp)
AGTTCT or AGGTCA
The diversity of the B. microti VNTRs on Nantucket Island, MA
Size range (bp)
No. failed amplification (%)
0.69 [0.63, 0.75]
0.45 [0.37, 0.52]
0.01 [0.01, 0.05]
0.28 [0.22, 0.41]
0.08 [0.04, 0.15]
Field sites sampled and the Simpson's index of diversity for identified genotypes
No. with multiple types
Established sites of transmission
0.98 [0.97, 0.98]
0.89 [0.85, 0.93]
0.51 [0.37, 0.63]
0.86 [0.79, 88]
0.80 [0.56, 0.85]
0.90 [0.84, 0.92]
0.90 [0.78, 0.90]
Mainland Rhode Island
Emerging sites of transmission
0.76 [0.51, 0.83]
0.71 [0.53, 0.83]
0.97 [0.96, 0.98]
Our capacity to describe the population structure of B. microti has been hindered by the conserved nature of its genes. Sequencing of B. microti 18SrDNA, beta-tubulin, and ITS have demonstrated limited sequence diversity globally  and we previously were not able to reliably discriminate strains from Nantucket and Spooner, Wisconsin. With the availability of the B. microti genome , we developed an assay for typing B. microti based on variable number tandem repeats (VNTRs). Tandem repeat regions (or minisatellites) are portions of genes which rapidly evolve due to slip strand errors in replication, and have been used as the basis for typing diverse organisms, providing valuable insight into the ecology and epidemiology of the related Babesia bovis, Theileria parva, and Plasmodium falciparum,. Our VNTR markers appear to be ideal for molecular epidemiologic studies to analyze the zoonotic B. microti epidemic over time and space. Leveraging an extensive archive of Nantucket field samples we find that two markers, BMV1 and BMV2 did not change at all (no apparent production of new alleles) over 26 years. In contrast, BMV4 was highly variable yielding 16 different alleles (Simpson's index of diversity = 0.69). This balance of stability and variability in our VNTR marker set allows for measuring diversity without the loss of genotypic characterization due to homoplasy.
Since the recognition of B. microti babesiosis on Nantucket Island during the early 1970s and its subsequent characterization as “Nantucket fever”, this island has reported a large proportion of all nationally recognized cases ,. The early force of B. microti transmission there stimulated the hypothesis that the guild of I. dammini-maintained microbes was originally to be found in the terminal moraine sites that were once contiguous with (given lower sea levels) or quickly reinvaded from southern refugia, likely Georges’ Island, after the retreat of the Laurentide ice sheet  about 6,000-12,000 years before present. Any newly recognized sites, particularly on mainland New England, were thought to represent introduction from original transmission sites such as Nantucket. Our analysis, however, clearly refutes anecdotal suggestions that Nantucket Island itself is the source of the recent geographic expansion of B. microti in New England. Parasites from the sites with emerging populations, Dover and Grafton, are more closely related to those from Connecticut and Rhode Island than those from Nantucket Island, a consistent finding by both methods of analysis (Structure and Phyloviz).
The haplotypes of B. microti collected across New England comprise at least two distinct populations. Parasites from the coastal islands of Massachusetts, Martha's Vineyard and Nantucket, along with samples from Great Island (25 km north of Nantucket, on Cape Cod) are grouped together (Nantucket group). Distinctly separate from these are all the parasites from Connecticut and Rhode Island, which includes samples from their coastal islands, Block Island and Prudence Island, (CT/RI group). The island of Martha's Vineyard yielded the most diverse population of parasites. Most of the samples clustered closely with those from Nantucket. However, Structure analysis groups some individual samples from Martha's Vineyard with CT/RI group, and for some samples no clear decision was made. Based on haplotypes, Phyloviz places these samples as singlets or doublets (unique haplotypes or those found only twice) with no clear connection to either main group. The presence of greater parasite diversity on Martha’s Vineyard may relate to the continuous survival of large tracts of oak woodland and scrubland for the past 1000 to 2000 years , whereas virtually all of Nantucket had been converted to sheep pasture by the mid 1800s. The deer tick was not present on either island during the first third of the 20th century. An arthropod survey on Nantucket  lists only Dermacentor variabilis and the description of I. muris (Nantucket is the type locality) does not mention other ticks . Intensive D. variabilis control programs on Martha’s Vineyard by Harvard’s Burt Wolbach during the late 1920s, continued by Marshall Hertig into the early 1940s , document the presence of “I. scapularis” on adjacent Naushon Island but not on the Vineyard, where I. muris was present. Indeed, in a seminal report describing “Cytoecetes microti”, now known as Anaplasma phagocytophilum, Tyzzer noted the presence of Babesia in splenectomized voles from Martha’s Vineyard, suggesting its enzootic transmission by I. muris. Parasites maintained by the nidicolous tick, I. muris, would have been limited in their local distribution with few opportunities for recombination, due to the confinement of all 3 stages on rodents or within their nests. With the establishment of the 3-host tick I. dammini, presumably from nearby Naushon , small isolated natural foci could expand due to dispersal by the more diverse I. dammini hosts; such foci would then coalesce across the adaptive landscape of each island, as we have suggested for the agent of tularemia on Martha’s Vineyard . Then too, the Nantucket landscape has changed even within the last 30 years, with greater portions of low-lying heathland overgrown by scrub oak thickets, thereby promoting denser populations of ticks and mice.
The history of zoonotic babesiosis due to B. microti is a function of the expanded distribution of dense infestations of I. dammini. (It is noted that many consider I. dammini to simply be I. scapularis , but northern populations are genetically more homogenous than are those from more southerly sites - which represent ticks with limited anthropophily ). The stability and intensity of populations of deer ticks depend on that of deer . Deer had largely been extirpated in New England by the late 1800s and forests were replaced by farmland  where the deer tick likely became very rare. Hence, early explanations of the origins of the Lyme disease and babesiosis epidemic emphasized the early report from Wolbach’s group  of the presence of “I. scapularis” on the Elizabeth Islands just north of Martha’s Vineyard because deer and forests had been protected there since the first days of colonization ; Naushon, the largest of the Elizabeth Islands, was thought to be the main postcolonial deer tick refugium until deer returned in numbers to other sites. Deer were, in fact, not completely extinct elsewhere in New England. Small remnant populations remained in numerous sites across New England, on Cape Cod, in the Berkshires, as well as sites in Connecticut and New York State . These sites could have maintained unnoticed deer tick populations with their microbial guild; the absence of reports of collections of deer ticks from elsewhere does not imply evidence of absence in such sites. Isolated collections of I. dammini were in fact documented from Long Island, New Haven CT, and Cape Cod MA in the 1930s, and as far inland as Walpole, MA in 1949 . Accordingly, the presence of a second major group of B. microti haplotypes (CT/RI) on the mainland should not be considered surprising. Infestations of deer ticks may have remained unrecognized until deer became locally abundant. Human babesiosis would emerge as the local force of transmission changed as a function of tick density, although increased suburbanization also was required to expose humans to infected ticks. A similar scenario of widely distributed relict populations across the northeastern U.S., with recent expansion from such cryptic sites as a result of greater deer tick density due to deer abundance and habitat change, has also been suggested for B. burgdorferi. Inasmuch as our study was limited to convenience samples from southern New England, it is likely that additional distinct populations will be found across the range of enzootic B. microti in the Northeast. It would be particularly illuminating to analyze parasites from Shelter Island (eastern Long Island), where babesiosis cases were first identified at nearly the same time as those on Nantucket and Martha’s Vineyard.
Interestingly, parasites from Sandy Neck, a 7 km long x 1 km wide barrier beach 8 km due north of Great Island, were quite unique and did not consistently group with either population. Based on allele frequences, Structure placed Sandy Neck parasites with the CT/RI population (Figure 4). However, the haplotypes found there were unique and significantly different than those found elsewhere (Figure 5). Sandy Neck formed no more than 4,000 years ago  from accretion on the Barnstable moraine and comprises old stands of maritime pitch pine and oak forest. It was one of the early Massachusetts sites with a stable infestation of I. dammini and ecological studies of the tick began there in 1983. The unique B. microti alleles suggest isolation from Cape Cod (the barrier beach is connected at one side and physically separated for most of its length by a great salt marsh) and southern New England in general. However, we only analyzed a limited number of samples from that site and none that were recently acquired, and thus additional sampling may disprove the hypothesis of allopatry.
The fact that Ipswich yielded B. microti haplotypes from both populations is puzzling. The Ipswich area is adjacent to a major stopover for migratory birds (Parker River National Wildlife Refuge ) and indeed Ipswich was the northernmost zoonotic site (Lyme disease and babesiosis) in New England during the late 1980s , with a large distributional gap between that site and southern Massachusetts. Northward transport of infected nymphal ticks by migratory birds would be the most logical hypothesis to explain the development of foci north of the coastal infestations. However, if such ticks fed to repletion and developed to adults in the new site, they would not feed on reservoir competent hosts because adult deer ticks have never been found on rodents or insectivores. Although the reservoir competence of mustelids and carnivores (both possible hosts for adult deer ticks) for B. microti has not been formally explored, we have never detected natural infection in skunks, raccoons (these two hosts have their own seemingly specific Babesia spp. ), domestic dogs, and cats (unpublished) and they are unlikely candidates to initiate transmission in a new site. Deer are not susceptible to B. microti infection  and co-feeding transmission on such an incompetent host would be unlikely unless babesial sporozoites are pluripotent and could quickly develop to gametocytes. Cottontail rabbits are infected by B. microti, and uncommonly serve as host for adult I. dammini,. It is possible that they could serve as the “bridging” host for introduction. Alternatively, infected nymphal deer ticks may not feed to repletion on a bird, detach, and infest rodents to complete feeding, thereby introducing B. microti to competent reservoirs in new sites. It is also possible that infected rodents or even infected host-seeking ticks are inadvertently transported within or on luggage or household goods of vacationers, introducing infection to their yards. Finally, avian piroplasms have been described  and we cannot exclude the hypothesis that certain birds may indeed be reservoir competent for B. microti without a formal experimental test. Given that there are several possible means of introduction, it is likely that the currently expanding pattern of zoonotic B. microti risk in southern New England reflects both geological and demographic history as well as transport. Additional analyses are required, with more comprehensive sampling schemes, to fully describe the basis for the expanded distribution of human babesiosis due to B. microti.
We demonstrate that enzootic B. microti in southern New England is comprised of two distinct populations. The Nantucket population is limited to Martha's Vineyard, Cape Cod and Nantucket Island in Massachusetts, and is not the source for newly emergent mainland sites of B. microti transmission. The CT/RI population is found in Connecticut and Rhode Island, including their coastal islands of Prudence and Block, as well as Ipswich, Massachusetts and newly emergent sites in mainland Massachusetts. Geology, postcolonial faunal and floral changes, and recent long distance transport events have all contributed to the current distribution of enzootic B. microti, but their relative contributions remain to be fully described.
Our work over the years has been generously supported by the National Institutes of Health (AI19693 to the late Andrew Spielman; AI 37993, 39002, 064218), the Evelyn Lilly Lutz Foundation, and the Dorothy Harrison Egan Foundation. We thank Dr. Tim Lepore of the Nantucket Cottage Hospital; Jim Lentowski of the Nantucket Conservation Foundation; the late Dr. Wesley N. Tiffney Jr. and Dr. Sarah Oktay of the UMass Field Station; Richard Johnson and the Martha’s Vineyard Tick Borne Disease Initiative; Dr. Barbara Roth-Schecter; Dr. Peter Auger; Dr. Mark Wilson; Dr. Tom Mather; and many field assistants over 30 years for their help in providing tick or rodent samples, or facilitating our work in the field. We dedicate this paper to Joseph Piesman, D.Sc., who recently retired as Chief of Lyme Disease Vector Activity at the Center for Disease Control and Prevention, Bacterial Zoonoses Branch, Fort Collins. Joe’s thesis work on the new babesiosis epidemic on Nantucket during the late 1970s served as the foundation for what we know today about its epidemiology and ecology. He taught the senior author babesiology and passed on his knowledge, Babesia isolates, data, and Nantucket field sites. This is a contribution of the University of Massachusetts Nantucket Field Station.
- Piesman J, Spielman A: Host-associations and seasonal abundance of immature Ixodes dammini in southeastern Massachusetts. Ann Entomol Soc Am. 1979, 72: 829-832.View ArticleGoogle Scholar
- Spielman A, Etkind P, Piesman J, Ruebush TK, Juranek DD, Jacobs MS: Reservoir hosts of human babesiosis on Nantucket Island. Am J Trop Med Hyg. 1981, 30: 560-565.PubMedGoogle Scholar
- Telford SR, Mather TN, Adler GH, Spielman A: Short-tailed shrews as reservoirs of the agents of Lyme disease and human babesiosis. J Parasitol. 1990, 76: 681-683.PubMedGoogle Scholar
- Telford SR, Gorenflot A, Brasseur P, Spielman A: Babesial infections of humans and wildlife. Parasitic Protozoa. Volume 5. 1993, Academic Press, New York, 1-47. 2Google Scholar
- Vannier E, Krause PJ: Human babesiosis. N Engl J Med. 2012, 366: 2397-2407. 10.1056/NEJMra1202018.View ArticlePubMedGoogle Scholar
- Stramer SL, Hollinger FB, Katz LM, Kleinman S, Metzel PS, Gregory KR, Dodd RY: Emerging infectious disease agents and their potential threat to transfusion safety. Transfusion (Paris). 2009, 49: 1S-29S. 10.1111/j.1537-2995.2009.02279.x.View ArticleGoogle Scholar
- Acosta MEP, Ender PT, Smith EM, Jahre JA:Babesia microti infection, Eastern Pennsylvania, USA. Emerg Infect Dis. 2013, 19: 1105-1107. 10.3201/eid1907.121593.PubMed CentralView ArticlePubMedGoogle Scholar
- Herwaldt BL, McGovern PC, Gerwel MP, Easton RM, MacGregor RR: Endemic babesiosis in another eastern state: New Jersey. Emerg Infect Dis. 2003, 9: 184-10.3201/eid0902.020271.PubMed CentralView ArticlePubMedGoogle Scholar
- Stafford KC, Williams SC, Magnarelli LA, Bharadwaj A, Ertel S-H, Nelson RS: Expansion of zoonotic babesiosis and reported human cases, Connecticut, 2001–2010. J Med Entomol. 2014, 51: 245-252. 10.1603/ME13154.View ArticleGoogle Scholar
- Pfeiffer CD, Kazmierczak JJ, Davis JP: Epidemiologic features of human babesiosis in Wisconsin, 1996–2005. WMJ Off Publ State Med Soc Wis. 2007, 106: 191-195.Google Scholar
- Tonnetti L, Thorp AM, Deisting B, Bachowski G, Johnson ST, Wey AR, Hodges JS, Leiby DA, Mair D: Babesia microti seroprevalence in Minnesota blood donors. Transfusion (Paris). 2013, 53: 1698-1705. 10.1111/j.1537-2995.2012.03948.x.View ArticleGoogle Scholar
- Joseph JT, Roy SS, Shams N, Visintainer P, Nadelman RB, Hosur S, Nelson J, Wormser GP: Babesiosis in lower Hudson Valley, New York, USA. Emerg Infect Dis. 2011, 17: 843-847. 10.3201/eid1705.101334.PubMed CentralView ArticlePubMedGoogle Scholar
- Cornillot E, Hadj-Kaddour K, Dassouli A, Noel B, Ranwez V, Vacherie B, Augagneur Y, Brès V, Duclos A, Randazzo S, Carcy B, Debierre-Grockiego F, Delbecq S, Moubri-Ménage K, Shams-Eldin H, Usmani-Brown S, Bringaud F, Wincker P, Vivarès CP, Schwarz RT, Schetters TP, Krause PJ, Gorenflot A, Berry V, Barbe V, Mamoun CB: Sequencing of the smallest Apicomplexan genome from the human pathogen Babesia microti. Nucleic Acids Res. 2012, 40: 9102-9114. 10.1093/nar/gks700.PubMed CentralView ArticlePubMedGoogle Scholar
- Matyas BT, Nieder HS, Telford SR: Pneumonic tularemia on Martha’s Vineyard. Ann N Y Acad Sci. 2007, 1105: 351-377. 10.1196/annals.1409.013.View ArticlePubMedGoogle Scholar
- Spielman A, Wilson ML, Levine JF, Piesman J: Ecology of Ixodes dammini-borne human babesiosis and Lyme disease. Annu Rev Entomol. 1985, 30: 439-460. 10.1146/annurev.en.30.010185.002255.View ArticlePubMedGoogle Scholar
- Dammin GJ, Spielman A, Benach JL, Piesman J: The rising incidence of clinical Babesia microti infection. Hum Pathol. 1981, 12: 398-400. 10.1016/S0046-8177(81)80020-2.View ArticlePubMedGoogle Scholar
- Anderson JF, Magnarelli LA, Kurz J: Intraerythrocytic parasites in rodent populations of Connecticut: Babesia and Grahamella Species. J Parasitol. 1979, 65: 599-604. 10.2307/3280327.View ArticlePubMedGoogle Scholar
- Piesman J, Mather TN, Donahue J, Levine J, Campbell JD, Karakashian SJ, Spielman A: Comparative prevalence of Babesia microti and Borrelia burgdorferi in four populations of Ixodes dammini in eastern Massachusetts. Acta Trop. 1986, 43: 263-270.PubMedGoogle Scholar
- Piesman J: Ixodes dammini: Its role in transmitting Babesia microti to man. 1980, Doctoral thesis, Harvard School of Public Health, Boston, MAGoogle Scholar
- Truett GE, Heeger P, Mynatt RL, Truett AA, Walker JA, Warman ML: Preparation of PCR quality mouse genomic DNA with hot sodium hydroxide and Tris (HotSHOT). Biotechniques. 2000, 29: 52-54.PubMedGoogle Scholar
- Ohmori S, Kawai A, Takada N, Saito-Ito A: Development of real-time PCR assay for differential detection and quantification for multiple Babesia microti-genotypes. Parasitol Int. 2011, 60: 403-409. 10.1016/j.parint.2011.06.021.View ArticlePubMedGoogle Scholar
- Rozen S, Skaletsky H: Primer3. ; 1998.Google Scholar
- Toonen RJ, Hughes S: Increased throughput for fragment analysis on an ABI PRISM 377 automated sequencer using a membrane comb and STRand software. BioTechniques. 2001, 31: 1320-1324.PubMedGoogle Scholar
- Rudzinska MA, Spielman A, Lewengrub S, Trager W, Piesman J: Sexuality in piroplasms as revealed by electron microscopy in Babesia microti. Proc Natl Acad Sci. 1983, 80: 2966-2970. 10.1073/pnas.80.10.2966.PubMed CentralView ArticlePubMedGoogle Scholar
- Tibayrenc M, Ayala FJ: The clonal theory of parasitic protozoa: 12 years on. Trends Parasitol. 2002, 18: 405-410. 10.1016/S1471-4922(02)02357-7.View ArticlePubMedGoogle Scholar
- Ross A, Koepfli C, Li X, Schoepflin S, Siba P, Mueller I, Felger I, Smith T: Estimating the numbers of malaria infections in blood samples using high-resolution genotyping data. PLoS One. 2012, 7: e42496-10.1371/journal.pone.0042496.PubMed CentralView ArticlePubMedGoogle Scholar
- Van den Eede P, Van der Auwera G, Delgado C, Huyse T, Soto-Calle VE, Gamboa D, Grande T, Rodriguez H, Llanos A, Anne J, Erhart A, D’Alessandro U: Multilocus genotyping reveals high heterogeneity and strong local population structure of the Plasmodium vivax population in the Peruvian Amazon. Malar J. 2010, 9: 151-10.1186/1475-2875-9-151.PubMed CentralView ArticlePubMedGoogle Scholar
- Piesman J, Karakashian SJ, Lewengrub S, Rudzinska MA, Spielman A: Development of Babesia microti sporozoites in adult Ixodes dammini. Int J Parasitol. 1986, 16: 381-385. 10.1016/0020-7519(86)90118-9.View ArticlePubMedGoogle Scholar
- Hammer Ø, Harper DAT, Ryan PD: PAST: paleontological statistics software package for education and data analysis. Palaeontol Electron. 2001, 4: 1-9.Google Scholar
- Pritchard JK, Stephens M, Donnelly P: Inference of population structure using multilocus genotype data. Genetics. 2000, 155: 945-959.PubMed CentralPubMedGoogle Scholar
- Evanno G, Regnaut S, Goudet J: Detecting the number of clusters of individuals using the software structure: a simulation study. Mol Ecol. 2005, 14: 2611-2620. 10.1111/j.1365-294X.2005.02553.x.View ArticlePubMedGoogle Scholar
- Francisco AP, Vaz C, Monteiro PT, Melo-Cristino J, Ramirez M, Carriço JA: PHYLOViZ: phylogenetic inference and data visualization for sequence based typing methods. BMC Bioinformatics. 2012, 13: 87-10.1186/1471-2105-13-87.PubMed CentralView ArticlePubMedGoogle Scholar
- Goethert HK, Telford SR: What is Babesia microti?. Parasitology. 2003, 127: 301-309. 10.1017/S0031182003003822.View ArticlePubMedGoogle Scholar
- Simuunza M, Bilgic H, Karagenc T, Syakalima M, Shiels B, Tait A, Weir W: Population genetic analysis and sub-structuring in Babesia bovis. Mol Biochem Parasitol. 2011, 177: 106-115. 10.1016/j.molbiopara.2011.02.002.View ArticlePubMedGoogle Scholar
- Muleya W, Namangala B, Simuunza M, Nakao R, Inoue N, Kimura T, Ito K, Sugimoto C, Sawa H: Population genetic analysis and sub-structuring of Theileria parva in the northern and eastern parts of Zambia. Parasit Vectors. 2012, 5: 1-11. 10.1186/1756-3305-5-1.View ArticleGoogle Scholar
- Anderson TJC, Haubold B, Williams JT, Estrada-Franco§ JG, Richardson L, Mollinedo R, Bockarie M, Mokili J, Mharakurwa S, French N, Whitworth J, Velez ID, Brockman AH, Nosten F, Ferreira MU, Day KP: Microsatellite markers reveal a spectrum of population structures in the malaria parasite Plasmodium falciparum. Mol Biol Evol. 2000, 17: 1467-1482. 10.1093/oxfordjournals.molbev.a026247.View ArticlePubMedGoogle Scholar
- Gunawardena S, Karunaweera ND, Ferreira MU, Phone-Kyaw M, Pollack RJ, Alifrangis M, Rajakaruna RS, Konradsen F, Amerasinghe PH, Schousboe ML, Galappaththy GNL, Abeyasinghe RR, Hartl DL, Wirth DF: Geographic structure of Plasmodium vivax: microsatellite analysis of parasite populations from Sri Lanka, Myanmar, and Ethiopia. Am J Trop Med Hyg. 2010, 82: 235-242. 10.4269/ajtmh.2010.09-0588.PubMed CentralView ArticlePubMedGoogle Scholar
- Herwaldt BL, Montgomery S, Woodhall D, Bosserman E: Babesiosis surveillance — 18 states, 2011. Morb Mortal Wkly Rep. 2012, 61: 505-509.Google Scholar
- Massachusetts Department of Public Health: www.mass.gov/eohhs/docs/dph/cdc/babesiosis-surveillance-2013.pdf, accessed November 1, 2014
- Telford SR, Spielman A: Enzootic transmission of Babesia microti. Tick Borne Pathog Host-Vector Interface. 1992, University of Minnesota College of Agriculture, St. Paul, MN, 259-264.Google Scholar
- Foster D, Motzkin G: Historical Influences on the Landscape of Martha’s Vineyard: Perspecitves on the Management of the Manuel F. Correllus State Forest. 1999, Harvard University: [Harvard Forest Paper, vol. #23], Petersham, MassachusettsGoogle Scholar
- Johnson C: A list of the insect fauna of Nantucket, MA. Proc Maria Mitchell Assoc Nantucket 1930, III.,Google Scholar
- Bishopp FC, Smith CN: A new species of Ixodes from Massachusetts. Proceeding Entomol Soc Wash. 1937, 39: 133-138.Google Scholar
- Hertig M, Smiley D: The problem of controlling wood ticks on Martha’s Vineyard. The Vineyard Gazette. 1937, 92 (36): 1-10.Google Scholar
- Telford SR, Dawson JE, Katavolos P, Warner CK, Kolbert CP, Persing DH: Perpetuation of the agent of human granulocytic ehrlichiosis in a deer tick-rodent cycle. Proc Natl Acad Sci. 1996, 93: 6209-6214. 10.1073/pnas.93.12.6209.PubMed CentralView ArticlePubMedGoogle Scholar
- Tyzzer EE:Cytoecetes microti, n. g., n. sp., a parasite developing in granulocytes and infective for small rodents. Parasitology. 1938, 30: 242-257. 10.1017/S0031182000025774.View ArticleGoogle Scholar
- Spielman A, Clifford CM, Piesman J, Corwin MD: Human babesiosis on Nantucket Island, USA: description of the vector, Ixodes (Ixodes) dammini, n. sp. (Acarina: Ixodidae). J Med Entomol. 1979, 15: 218-234.View ArticlePubMedGoogle Scholar
- Goethert HK, Shani I, Telford SR: Genotypic diversity of Francisella tularensis infecting Dermacentor variabilis ticks on Martha’s Vineyard, Massachusetts. J Clin Microbiol. 2004, 42: 4968-4973. 10.1128/JCM.42.11.4968-4973.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Sakamoto JM, Goddard J, Rasgon JL: Population and demographic structure of Ixodes scapularis Say in the eastern United States. PLoS One. 2014, 9: e101389-10.1371/journal.pone.0101389.PubMed CentralView ArticlePubMedGoogle Scholar
- Rich SM, Caporale DA, Telford SR, Kocher TD, Hartl DL, Spielman A: Distribution of Ixodes ricinus-like ticks in eastern North America. Proc Natl Acad Sci U S A. 1995, 92: 6284-6288. 10.1073/pnas.92.14.6284.PubMed CentralView ArticlePubMedGoogle Scholar
- Rosenthal BM, Spielman A: Reduced variation among northern deer tick populations at an autosomal microsatellite locus. J Vector Ecology. 2004, 29: 227-235.Google Scholar
- Telford S: The name Ixodes dammini epidemiologically justified. Emerg Infect Dis. 1998, 4: 132-134. 10.3201/eid0401.980126.PubMed CentralView ArticlePubMedGoogle Scholar
- Telford SR: Deer tick transmitted zoonoses in the eastern United States. Conserv Med Ecol Health Pract. 2002, Oxford University Press, New York, 310-324.Google Scholar
- Cronon W: Changes in the Land. 1983, Hill and Wang, New YorkGoogle Scholar
- Larrousse F, King AG, Wolbach SB: The overwintering in Massachusetts of Ixodiphagus caucurtei. Science. 1928, 67: 351-353. 10.1126/science.67.1735.351.View ArticlePubMedGoogle Scholar
- Spielman A: Lyme disease and human babesiosis: evidence incriminating vector and reservoir hosts. Pp 147–165. Biology of Parasitism: A Molecular and Immunological Approach. Edited by: Englund PT, Sher A, Alan R. 1988, Liss Inc, NY,Google Scholar
- Severinghaus CW, Brown CP: History of the white-tailed deer in New York. N Y Fish Game J. 1956, 3: 129-Google Scholar
- Hoen AG, Margos G, Bent SJ, Diuk-Wasser MA, Barbour A, Kurtenbach K, Fish D: Phylogeography of Borrelia burgdorferi in the eastern United States reflects multiple independent Lyme disease emergence events. Proc Natl Acad Sci. 2009, 106: 15013-15018. 10.1073/pnas.0903810106.PubMed CentralView ArticlePubMedGoogle Scholar
- Redfield A: Ontogeny of a salt marsh estuary. Science. 1965, 147: 50-55. 10.1126/science.147.3653.50.View ArticlePubMedGoogle Scholar
- Carson RL: Parker river: a national wildlife refuge. Conservation in Action. 1947, 2: 1-15.Google Scholar
- Lastavica CC, Wilson ML, Berardi VP, Spielman A, Deblinger RD: Rapid emergence of a focal epidemic of Lyme disease in coastal Massachusetts. N Engl J Med. 1989, 320: 133-137. 10.1056/NEJM198901193200301.View ArticlePubMedGoogle Scholar
- Piesman J, Spielman A, Etkind P, Ruebush TK, Juranek D: Role of deer in the epizootiology of Babesia microti in Massachusetts, USA. J Med Entomol. 1979, 15: 537-540.View ArticlePubMedGoogle Scholar
- Goethert HK, Telford SR: Enzootic transmission of Babesia divergens among cottontail rabbits on Nantucket Island, Massachusetts. Am J Trop Med Hyg. 2003, 69: 455-460.PubMedGoogle Scholar
- Telford SR, Spielman A: Enzootic transmission of the agent of Lyme disease in rabbits. Am J Trop Med Hyg. 1989, 41: 482-490.PubMedGoogle Scholar
- Peirce MA:Nuttallia França, 1909 (Babesiidae) preoccupied by Nuttallia Dall, 1898 (Psammobiidae): a re-appraisal of the taxonomic position of the avian piroplasms. Int J Parasitol. 1975, 5: 285-287. 10.1016/0020-7519(75)90075-2.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.