- Open Access
Exploring features and function of Ss-riok-3, an enigmatic kinase gene from Strongyloides stercoralis
© Wang et al.; licensee BioMed Central. 2014
- Received: 28 August 2014
- Accepted: 23 November 2014
- Published: 5 December 2014
Right open reading frame protein kinase 3 (RIOK-3) belongs to the atypical kinase family. Unlike the other two members, RIOK-1 and RIOK-2, which are conserved from Archaea to humans, RIOK-3 occurs only in multicellular organisms. Studies on HeLa cells indicate that human RIOK-3 is a component of the 40S small ribosome subunit and supports cancer cell growth and survival. However, almost nothing is known about the function of RIOK-3. We explored the functional role of RIOK-3 encoding gene from Strongyloides stercoralis, a parasitic nematode of humans and dogs.
To analyze the gene and promoter structure of Ss-riok-3, RACE-PCR and Genome-walker PCR were performed to isolate the full length cDNA, gDNA and promoter region of Ss-riok-3. RNA-seq was conducted to assess the transcript abundance of Ss-riok-3 in different stages of S. stercoralis. Transgenesis was employed to determine the anatomic expression patterns of Ss-riok-3.
The RIOK-3 protein-encoding gene (designated Ss-riok-3) of S. stercoralis was characterized. The full-length complementary and genomic DNAs of the RIOK-3 encoding gene (riok-3) were isolated from this nematode. The cDNA of Ss-riok-3 is 1,757 bp in length, including a 23 bp 5’-UTR, a 36 bp 3’-UTR and a 1,698 bp coding region encoding a protein of 565 amino acids (aa) containing a RIO kinase domain. RNA sequencing (RNA-seq) analysis revealed that Ss-riok-3 is transcribed in all developmental stages of S. stercoralis assessed, with transcripts being particularly abundant in parasitic females. Gene structure analysis revealed that Ss-riok-3 contains no intron. The putative promoter contains conserved promoter elements, including four TATA, two GATA, one inverse GATA and one inverse CAAT boxes. The promoter of Ss-riok-3 drives GFP expression in the head neuron, intestine and body wall muscle of transgenic S. stercoralis larvae, and the TATA boxes present in the 3’-UTR of the gene immediately upstream of Ss-riok-3 initiate transcription.
The characterization of the RIOK-3 encoding gene from S. stercoralis provides a sound foundation for investigating in detail its function in the development and reproduction of this important pathogen.
- Parasitic nematode
- Strongyloides stercoralis
Strongyloides stercoralis is a parasitic nematode of humans and dogs, affecting estimated 60–100 million people worldwide and causing strongyloidiasis . The life cycle of S. stercoralis is more complicated than those of most obligatory parasitic nematodes. Parasitic females (P Female) live in the intestine of the host and reproduce by mitotic parthenogenesis. They lay sexually differentiated eggs; these eggs hatch in the intestine, and post-parasitic first-stage larvae (PP L1) are either passed in the feces, or develop directly to auto-infective L3 (aiL3) within the host intestine. Then aiL3 invade somatic tissues and return to the intestine to produce a new generation of parasitic female adults (P Female). S. stercoralis undergoes low levels of autoinfection in immuno-competent hosts, however this process of autoinfection may proceed for sequential generations in an immuno-compromised host, with geometric expansion of parasite numbers and involvement of multiple body tissues, possibly leading to a fatal outcome . In immune-competent hosts, PP L1 pass in feces from the host, and female PP L1 develop either homogonically to infective third-stage larvae (iL3) or heterogonically to free-living female adults (FL Female), whereas the male PP L1 develop to free-living male adults (FL Male) invariably. The FL Female and FL Male mate and produce embryos, which develop to iL3 through post free-living L1 (PFL L1); iL3 infect the host by skin penetration and develop to P Female .
The complex life cycle of S. stercoralis and lack of knowledge about its developmental biology has hampered the progress to control strongyloidiasis, requiring us to elucidate key biological processes in the development. Studies of the essential molecules that regulate the development of parasitic nematodes, including S. stercoralis, could facilitate the discovery of new interventions to control strongyloidiasis and other nematodiases.
One group of molecules essential in eukaryotic organisms is the protein kinases. Such kinases are key enzymes required for the regulation of a wide range of cellular processes, including cell-cycle progression, transcription, DNA replication and/or metabolic processes . Based on their kinase domain sequences, protein kinases can be classified as eukaryotic protein kinases (ePKs) and atypical protein kinases (aPKs). In the human genome, 13 aPK families have been defined thus far, and one of them represents the family of the RIO protein kinases . Four members of the RIO protein kinase family have been reported, i.e. RIOK-1, RIOK-2, RIOK-3 and RIOK-B. RIOK-1 and RIOK-2 are conserved from Archaea to humans, whereas RIOK-3 is only present in multicellular eukaryotes and, interestingly, RIOK-B is found in some bacteria . RIOK-1, RIOK-2 and RIOK-3 contain a conserved RIO domain comprising multiple conserved functional motifs . However, the sequences of these motifs differ among the three RIO kinases with the RIO domain of RIOK-1 and RIOK-3 being more similar in sequence. External to their RIO domain, RIOK-2 and RIOK-3 contain an N-terminal domain which is absent from RIOK-1 . Consistent with these structural differences, the functions of the eukaryotic RIO kinases are also different. Although both RIOK-1 and RIOK-2 are involved in the maturation of the 40S ribosomal subunit including rRNA maturation and recycling other trans-acting factors involved in the maturation of 40S subunit, their functions therein are different, and they do not complement each other in yeast and human cells -.
The function of RIOK-3 appears to diverge markedly from those of RIOK-1 and RIOK-2. In spite of the report that human RIOK-3 is also a component of 40S ribosomal subunit particles, its function in ribosomal synthesis is still unknown . Human RIOK-3 is over-expressed in cancer cells - and interacts with caspase-10 to down-regulate NF-κB signaling by competing with receptor-interacting protein 1 (RIP1) and NF-κB-inducing kinase (NIK) . These findings suggest that RIOK-3 is necessary for human cancer cell growth and survival -. In spite of the functional importance of RIOK-3 in human cells, nothing is known about the function of this molecule in nematodes, except for the early studies from RNAi screens in free-living nematode Caenorhabditis elegans-, which have revealed no visible phenotypes including embryonic and larvae lethal, embryonic development variant, larvae developmental arrest, maternal sterile, organism morphology variant for the gene Ce-riok-3 (data from WormBase, http://www.wormbase.org/#01-23-6). This information suggests that the function of Ce-riok-3 in C. elegans is redundant. Whether riok-3 function can be replaced and/or supported by other genes in parasites is still unknown.
Although RNAi is an efficient tool for the analysis of gene function in C. elegans, this approach does not work well in parasitic nematodes of animals studied to date . Without effective RNAi-based methods, transgenesis constitutes an alternative approach for the functional genomic analysis of parasitic nematodes such as S. stercoralis-. In the present study, we explored RIOK-3 encoding gene Ss-riok-3 of S. stercoralis, and investigated its temporal and spatial expression patterns towards the ultimate goals of uncovering its function and understanding its role in S. stercoralis.
The S. stercoralis (UPD strain) was maintained in prednisolone-treated Beagles, in accordance with a protocol (permit no. SYXK-0029) approved by the Animal Ethics and Animal Experimentation Committee of Hubei Province. The care and maintenance of dogs were in strict accordance with the regulations for the Administration of Affairs Concerning Experimental Animals of P. R. China.
Parasite maintenance and culture
The UPD strain of S. stercoralis was maintained in immune-suppressed dogs ; iL3 were collected from 7-10 day-old copro-cultures (22°C) via Baermann funnel and washed with sterile buffered saline (BU) -. Free-living adults of S. stercoralis were collected via Baermann funnel from copro-cultures after 2 days of incubation at 22°C and then were placed on nematode growth medium (NGM) plates and maintained on Escherichia coli OP50.
Preparation of nucleic acids
Nucleic acids were isolated from 10,000–20,000 iL3s by a small-scale sodium proteinase K extraction  followed by purification using a mini-column (Wizard DNA Clean-Up System, Promega, USA). RNA was extracted from 20,000 ~ 30,000 iL3s using by TRizol reagent extraction (Life Technologies, USA). RNA yields were estimated spectrophotometrically (NanoDrop Technologies, Thermo Scientific, USA), and 1 μg of RNA was reverse transcribed to cDNA using a kit (Smart RACE Kit, Clontech, USA). Nucleic acids were stored at -80°C (RNA) or -20°C (DNA) until use.
Isolating cDNA and promoter elements of Ss-riok-3
Two primers 1 F and 2R (Additional file 1) were designed to the sequences of three expressed sequence tags (ESTs) for Ss-riok-3 (GenBank accession nos. BE224215, BE223756.1 and BG224329.1) from National Center for Biotechnology Information (NCBI, http://www.ncbi.nlm.nih.gov/). Using this primer pair, a region (255 bp) was PCR-amplified from cDNA transcribed from total RNA from iL3s of S. stercoralis, T/A-cloned into the vector pMD19T (Takara Biotechnology, Dalian, China) and directly sequenced. Based on the sequence obtained, gene-specific primers (3 F and 4R) were designed (Additional file 1). Using this pair of gene-specific primers and adaptor primers (from the RACE Amplification Kit, cat. no. 634923, Clontech), two partially overlapping cDNA fragments were produced from total RNA of S. stercoralis by 5’- and 3’-RACE, respectively. After sequencing the two partial cDNAs, three gene-specific primers (5 F, 6R and 8R) were designed to amplify the 5’- and 3’-termini of the Ss-riok-3 cDNA (Additional file 1). The complete cDNA of Ss-riok-3 was assembled using the sequences derived from the 5’-RACE and 3’-RACE amplicons and was searched and compared with the S. stercoralis genomic contigs (6 December 2011 draft; ftp://ftp.sanger.ac.uk/pub/pathogens/HGI/) by Geneious version 5.5.6 (http://www.geneious.com/). Subsequently, a pair of primers with restriction sites (Ss-riok3-Eco RI, Ss-riok3-Hind III) was designed to PCR-amplify the coding sequence (CDS) of Ss-riok-3 using the Advantage 2 Polymerase Mix (Clontech), employing the following cycling conditions: initial 94°C, 5 min; then 94°C, 30 s, 55°C, 30 s; 72°C, 90 s for 30 cycles; final extension at 72°C, 10 min. The resultant amplicon was then (T/A) cloned into pMD19-T and sequenced.
To identify the Ss-riok-3 promoter, four genomic DNA libraries were constructed using the Genome-Walker Kit (cat. no. 638904, BD Bioscience, Clontech) following the manufacturer’s instructions. Briefly, genomic DNA was digested with each of four restriction enzymes Dra I, EcoR V, Pvu II and Stu I (BD Biosciences, Clontech). Each of the four digested products was purified by phenol/chloroform extraction and then linked to an adapter with T4 DNA ligase. Then, PCR was performed using one adapter sense-primer and one gene-specific antisense-primer 10R (Additional file 1). All PCRs were conducted using the BD Advantage 2 Polymerase Mix (cat. no. 639201, Clontech) using the recommended touch-down PCR cycling protocol: 7 cycles at 94°C, 25 sec; 72°C, 3 min; 32 cycles at 94°C, 25 sec, 67°C, 3 min; final extension at 67°C for 7 min. The amplicons from all four genomic DNA libraries were examined separately on agarose gels; the largest products were purified employing TIANGEN Gel DNA purification kit (Tiangen Biotech, Beijing, China) and then (T/A) cloned into the vector pMD19-T (Takara Biotechnology) for subsequent sequencing.
To isolate the 3’ UTR of Ss-riok-3 upstream gene (Ss-rep-1), the 3’-RACE was performed with one gene specific sense primer (Ss-rep1-3 F, Additional file 1) and the adapter primer (RACE Amplification Kit, Clontech). The sense primer Ss-rep1-3 F was designed based on the sequence obtained by Genome-Walker PCR. 3’-RACE PCR was performed using the Advantage 2 Polymerase Mix (Clontech) with following protocol: initial 94°C, 5 min; then 94°C, 30 s, 55°C, 30 s; 72°C, 90 s for 30 cycles; final extension at 72°C, 10 min. The PCR product was (T/A) cloned into pMD-19 T and sequenced. After confirming the end of the 3’ UTR of Ss-rep-1 by aligning the cDNA with gDNA, two sense-primers, Ss-riok3pro1-Hind III and Ss-riok3pro2-Hind III and one anti-sense primer Ss-riok3pro-Sma I (Additional file 1) were designed to isolate the promoter region of Ss-riok-3, and the anti-sense primer Ss-riok3pro-Sma I was designed downstream (374 bp) of the start codon of Ss-riok-3 and positioned according to the 91 nucleotides including a 42 bp artificial intron (see Additional file 2) between Sma I restrict site and the start codon of gfp of the promoter-less plasmid pAJ 02, in order to match the codon order. Each of the two sense-primers was used with their respective anti-sense primer to PCR-amplify 683 bp or 881 bp of genomic DNA upstream of Ss-riok-3 (Additional file 2). The following cycling protocol was used: initial at 94°C for 3 min; then 94°C, 30 s; 72°C, 3 min for 5 cycles, followed by 94°C, 30 s; 67°C, 3 min for 25 cycles, and a final extension of 67°C, 7 min. Each amplicon was then (T/A) cloned into pMD19-T for sequencing.
The cDNA sequence of Ss-riok-3 was compared with publicly available sequence data in non-redundant databases from NCBI (http://www.ncbi.nlm.nih.gov/) using BlastX . The Ss-riok-3 cDNA was translated into a predicted amino acid (aa) sequence (Ss-RIOK-3) using the program BioEdit (http://www.mbio.ncsu.edu/BioEdit/bioedit.html#downloads). Protein motifs of Ss-RIOK-3 were predicted by interrogating the databases PROSITE  (www.expasy.ch/tools/scnpsit1.html) and Pfam  (www.sanger.ac.uk/Software/Pfam/). The sequence of Ss-RIOK-3 and its homologs from selected species were aligned using the program MAFFT 7.0  (http://mafft.cbrc.jp/alignment/software/). The functional domains and sub-domains were then identified in aligned RIOK3 sequences and then highlighted using the program Photoshop CS v.5.0.
The genomic sequence upstream of Ce-riok-3 was retrieved from WormBase (WS243, http://www.wormbase.org/); promoter elements of Ss-riok-3 and Ce-riok-3, and their respective upstream genes were predicted using the transcription element search system MatrixCatch (http://www.gene-regulation.com/cgi-bin/mcatch/MatrixCatch.pl) . The two putative promoter sequences were aligned using MAFFT 7.0 .
For phylogenetic analysis, Ss-RIOK-3 was searched in the non-redundant database of NCBI (http://www.ncbi.nlm.nih.gov/) and 18 homologous sequences were retrieved from six nematodes [Ascaris suum (ERG84434.1), Caenorhabditis brenneri (EGT33672.1), C. elegans (NP_499173.1), C. remanei (XP_003113164.1), Haemonchus contortus (ADW27446.1), Loa loa (XP_003136543.1)]; two arthropods [Aedes aegypti (XP_001660046.1) and Drosophila melanogaster (AAF50965.1)]; nine vertebrates [Canis lupus familiaris (XP_005623064.1), Danio rerio (NP_001003614.1), Homo sapiens (NP_003822.2, EAX01152.1), Mus musculus (NP_077144.2), Pan troglodytes (XP_003953313.1), Rattus norvegicus (NP_001101893.1), Salmo salar (NP_001133982.1), Xenopus laevis (NP_001083392.1) and X. tropicalis (NP_001004996.1)]; and yeast [Schizosaccharomyces pombe (CAA15723.2)]. Sequences of these 18 RIOK-3 homologs were then aligned with that of Ss- RIOK-3 using Clustal X and manually adjusted. Phylogenetic analyses were conducted using the maximum likelihood (ML), maximum parsimony (MP) and neighbor-joining (NJ) methods based on the Jones-Taylor-Thornton (JTT) model in the software package MEGA v.5.0 . Confidence limits were assessed by bootstrapping using 1,000 pseudo-replicates for ML, MP and NJ; other settings were obtained using the default values in MEGA v.5.0 . A 50% cut-off value was implemented for the consensus tree.
The S. stercoralis (PV001 line), derived from a single female worm of the UPD strain, was maintained as described previously ,-. Transcription profiles of Ss-riok-3 were assessed in seven developmental stages of the PV001 line of S. stercoralis, including free-living females (FL Female), post-free-living first-stage larvae (PFL L1), infective third-stage larvae (iL3), in vivo activated third-stage larvae (L3+), parasitic females (P Female), post-parasitic first-stage larvae (PP L1) and post-parasitic third-stage larvae (PP L3). The parasites from different stages were isolated as described previously ,. Transcription was quantified using RNA-seq . Raw RNA-seq data for the seven developmental stages are available at http://www.ebi.ac.uk/arrayexpress/ under accession numbers E-MTAB-2192 (iL3) and E-MTAB-1164 (FL Female, PFL L1, L3+, P Female, PP L1, and PP L3). Ss-riok-3-specific transcript abundances were calculated as fragments per kilobase of coding exon per million fragments mapped (FPKM), and significant differences in FPKM values between developmental stages were determined using Cufflinks v.2.0.2 (http://cufflinks.cbcb.umd.edu/) ,-; p-values <0.05 were considered statistically significant, and p-values <0.001 were considered statistically highly significant. FPKM and 95% confidence intervals were plotted in Prism version 5.01 (GraphPad Software, Inc., http://www.graphpad.com/).
Transformation constructs and transformation of S. stercoralis
To make reporter transgene constructs, alternative promoter regions of Ss-riok-3 (Additional file 2), 683 bp and 881 bp, respectively, were digested with the endonucleases Hind III and Sam I (Thermo Fisher Scientific, USA) from the (T/A) cloned recombinant pMD19T plasmids and gel-purified using the Tiangen Gel Purification Kit (Tiangen Biotech). Each of the purified products was then sub-cloned into the promoter-less plasmid pAJ 02 , upstream of the gfp CDS, to create two plasmids: pRP2: Ss-riok-3p (683 bp)::gfp::Ss-era-1 3’-UTR and pRP4: Ss-riok-3p (881 bp)::gfp::Ss-era-1 3’-UTR (Additional file 2). The inserts of the plasmids were sequenced. After confirming the sequences, the two constructs were isolated using the TIANpure Midi Plasmid Kit (Tiangen Biotech) and each of them was diluted to 50 ng/μl and stored at -20°C. FL Females were transformed by gonadal micro-injection as previously described . Briefly, solutions containing 50 ng/μL of plasmid pRP2 or pRP4 were injected into the gonads of FL Females. Transformed FL Females were then paired with one or two FL adult male(s) on an NGM + OP50 plates, and incubated at 22°C for subsequent egg laying. F1 post-free-living progeny were screened for fluorescence at 24, 48 and 72 h, respectively, after microinjection. S. stercoralis larvae were screened for expression of fluorescent reporter transgenes using a stereomicroscope (SZX12, Olympus) with epifluorescence. Then the S. stercoralis worms with GFP expression were placed on 2% agarose pad (Lonza, Basel, Switzerland), anesthetized using 50 mM levamisole (Sigma-Aldrich, USA) to examine in detail using a compound microscope (BX60, Olympus) with Nomarski Differential Interference Contrast (DIC) optics and epifluorescence. Larvae were imaged with a Spot RT Color digital camera and Spot Advanced image analysis software (Diagnostic Instruments Inc., USA). Captured images were processed using Photoshop CS v.5.0 as previously described . Image-processing algorithms, limited to brightness and contrast adjustments, were all applied in linear fashion across the entire image.
Characterization of Ss-riok-3 cDNA
The full-length cDNA of Ss-riok-3 is 1,757 bp in length comprising a 23 bp 5’-UTR, a 36 bp 3’-UTR and a 1698 bp coding sequence encoding 565 aa. A poly A tract, followed the 3’-UTR and the polyadenylation signal (AAUAAA) was found at the eighteenth nucleotide from the poly A tail. The predicted Ss-RIOK-3 protein exhibits low sequence identities (21.4–37.5%) to RIOK-3 s from various organisms, including other nematodes, fish, amphibians, vertebrates, with the highest identity (37.54%) being to As-RIOK-3 (ERG84434) from A. suum.
Evolutionary relationship of the predicted protein Ss-RIOK-3 to homologs from other species
Characterisation of the organization of the gene Ss-riok-3
Analysis of the predicted promoter region of Ss-riok-3
Transcriptional analysis of Ss-riok-3 in different developmental stages
Anatomic expression pattern
RIOKs are atypical protein kinases, with the first representative (RIOK-1) discovered in yeast in 1997 . All species contain at least two members of RIOK (i.e. RIOK-1 and RIOK-2), but multicellular, eukaryotic organisms studied to date also possess RIOK-3 . In human cells and yeast, RIOK-1 and RIOK-2 play important roles in the process of ribosomal biology, including recycling of the trans-acting factors and/or rRNA cleavage -,. Compared with RIOK-1 and RIOK-2, much less is known about RIOK-3. The function of RIOK-3 is different from those of RIOK-1 and RIOK-2 in human cells, with RIOK-3 controlling tumor cell invasiveness, suppressing the activity of NF-κB and supporting type I-interferon production ,,,. Recently, human RIOK-3 was discovered in ribosomal 40S subunit particles .
Currently, nothing is known about the function of RIOK-3 in nematodes. We have begun to rectify this by investigating the structure, transcription patterns and anatomic expression of Ss-riok-3, the RIOK-3-encoding gene from S. stercoralis. We identified only one transcript of Ss-riok-3; however, in C. elegans, there are two transcript variants (isoforms) of Ce-riok-3, with the same coding sequence but with 5’-UTRs of differing lengths (http://www.wormbase.org/). The human genome also encodes an additional riok-3 transcript variant, but the relevant locus is predicted to be a pseudo-gene . Consistent with our findings for S. stercoralis, riok-3 genes of other parasitic nematodes, including A. suum, Dirofilaria immitis and H. contortus, appear to produce only one transcript.
All four reported members from the RIO protein kinase family (i.e. RIOK-1, RIOK-2, RIOK-3 and RIOK-B) contain the conserved RIO domain, including an ATP binding motif, a flexible loop, a hinge, an active site and a metal-binding motif . The RIO domain is similarly conserved in Ss-RIOK-3. Like the RIOK-1 s and RIOK-2 s, RIOK-3 s also lacks the substrate-binding motif present in ePKs, but has an inserted flexible loop ,. It is interesting that the predicted peptide sequence of the ATP-binding motif of Ss- RIOK-3 has two aa substitutions compared with those of the other species studied herein. For example, the sequence is ASGKES in S. stercoralis and S. ratti, ATGKES in the other nematodes and STGKES in the non-nematode species selected. Analyses of RIOK-1 and RIOK-2 in a complex with ATP , reveal that the putative ATP binding motif, along with the beta strand-three, actually participate in binding ATP and that the Lys (K) located in the beta strand-three is required for this interaction. Site mutation changing the Lys (K) in the beta strand-three to Ala (A) in the ATP binding motifs of RIOK-2 and RIOK-3 in yeast and human cells ablate the ATP binding activity and affect the kinase functions of these enzymes ,. Although the Lys (K) from the ATP binding loop does not directly interact with ATP, it is essential for RIOK-2 to bind with pre-40S subunit to participate in ribosomal biogenesis . Whether the aa substitutions in the ATP-binding motif of Ss-RIOK-3 influence its affinity for ATP or AMP is unknown and warrants future investigation.
An alignment of predicted aa sequences (Figure 1) also revealed that RIOK-3 s from selected species, including Ss-RIOK-3, contain a N-terminal region that is much larger than the N-terminal regions of RIOK-1 s and RIOK-2 s. This finding is consistent with a comparative analysis of the predicted structures for RIOKs from C. elegans and parasitic nematodes for which extensive genomic and transcriptomic data sets are available . The precise function of this N-terminal region of RIOK-3 s is unknown; however, findings for RIOK-3 in human cells support that the N-terminal region of this RIOK-3 is involved in the binding of polyubiquitin and the suppression of TNFα-mediated NF-κB activity ,. In addition, bioinformatic analysis predicts that the N-terminal region of Ce-RIOK-3 is involved in transmembrane transport . In the present study, the Strongyloides RIOK-3 s (Ss-RIOK-3 and Sr-RIOK-3) contained a larger N-terminus than RIOK-3 s from other selected species; this largely reflects the low sequence identity between Strongyloides RIOK-3 and its homologs. This difference suggests that the N-terminus of Ss-RIOK-3 might have a unique function.
The analysis of the S. stercoralis transcriptome reveals that the transcription of Ss-riok-3 is high in the parasitic life stages of this worm, with the highest level in P Females. It also shows that, with the exception of iL3, transcripts of Ss-riok-3 are more abundant in FL Females than in post parasitic and post free-living larvae. The abundance of Ss-riok-3 transcripts in female worms suggests that Ss-riok-3 plays a functional role in reproduction. Due to the absence of transcripts from FL Males, we could not compare transcript abundance between FL Females and FL Males. However, as S. stercoralis is parthenogenetic, there is no male adult within the host. Nonetheless, the peak transcript abundance in P Females still suggests a key function for Ss-RIOK-3 in this developmental stage. In other parasitic nematodes (A. suum, B. malayi and H. contortus) riok-3 is transcribed at high levels in eggs but does not show significant differences between female and male adults in these three parasites . This information is consistent with trends of riok-3 transcription in embryos of C. elegans and parasitic nematodes, supporting the proposal of constitutive expression of this gene during embryogenesis ,.
Notably, Ss-riok-3 transcription is also enriched in L3+, which have invaded the host and resumed development to the P Female stage. The abundance of Ss-riok-3 transcript in the parasitic stages of S. stercoralis might indicate that Ss-RIOK-3 participates in sexual maturation of L3s developing to P Females. In C. elegans, analysis of gene expression cluster during sexual differentiation revealed that Ce-riok-3 is enriched in mature hermaphrodites . This trend is echoed by the enrichment of Ss-riok-3 transcripts in the P Females and FL Females of S. stercoralis, suggesting its possible roles in female reproductive biology of this nematode. The analyses of transcript abundance did not show transcriptional difference between FL Females and iL3, however, the promoter activity also shifts from intestine in PFL L1 to body wall muscle in iL3, which suggest that Ss-RIOK-3 could be involved in maintaining the activity of body wall muscle of iL3 to support host-finding and infection.
Ce-riok-3 is present in an operon (CEOP3628) . A similar genomic context is not supported for Ss-riok-3, as the donor SL1 and SL2 snoRNA is absent from the 5’ end of Ss-riok-3 cDNA, indicating no association with an operon; the gene immediately upstream of Ss-riok-3 (Ss-rep-1) is not found in the 5’-flanking region of Ce-riok-3. The intergenic region between the stop codon of Ss-rep-1 and the start codon of Ss-riok-3 is 423 bp. In the initial gene localization analysis, the 309 bp genomic DNA, comprising 23 bp of 5’-UTR of Ss-riok-3 and the 286 bp of intergenic region between the start codon of Ss-riok-3 and the end of 3’-UTR of Ss-rep-1 (Additional file 2), was predicted to be the promoter region of Ss-riok-3 and was thus cloned into the reporter gene plasmid to make a transformation construct pRP2 (Additional file 2). However, this region did not drive GFP expression in PFL L1-L2s (data not shown). Only the transformation construct (pRP4) comprising 423 bp genomic DNA region, including the 23 bp of 5’-UTR of Ss-riok-3, the 286 bp of intergenic genomic DNA and the 114 bp of 3’-UTR of Ss-rep-1, drove strong GFP expression in the intestine and head neuron of PFL L1s and L2s of S. stercoralis. The analysis of the potential promoter regions of Ss-riok-3 revealed that three of the four TATA boxes in the 5’-flanking region of Ss-riok-3 are actually located in the 3’-UTR of Ss-rep-1 which is immediately upstream of Ss-riok-3. Failure of the 683 bp promoter element in pRP2, which lacks these three TATA boxes, to drive gfp expression and the contrasting, robust reporter expression under control of the 881 bp promoter element in pRP4, which includes them, demonstrates that, despite their location within the regulatory sequences of another gene, the three TATA boxes in the 3’-UTR of Ss-rep-1 are essential for initiating transcription of Ss-riok-3 in vivo. The TATA box is a common promoter element, which is recognized by RNA polymerase II during transcription initiation in most protein-coding genes ,. Also present in the putative Ss-riok-3 promoter are four GATA and two inverse GATA boxes. The GATA transcription factor (TF) family consists of GATA 1 to 6 that contain zinc finger motif capable of binding to target DNA . GATA transcription factors regulate gene expression and play important roles in eukaryotic development -. In C. elegans, GATA TFs regulate gut-specific gene transcription, and ELT-2 TF predominantly controls the development of the intestine ,. The present results showed that the sequences of GATA boxes (TGATAA) in the predicted promoter region of Ss-riok-3 are consistent with ELT-2 binding sequence in C. elegans, which indicate GATA TFs regulate Ss-riok-3 transcription and expression in the intestine of PFL L1s.
The activity of the Ss-riok-3 promoter in the intestine of PFL L1-L2s is consistent with results of a high-throughput in vivo-analysis of gene expression in C. elegans, revealing that Ce-riok-3 is expressed in the intestine of larvae and adults ,. In C. elegans, the intestine is a relatively large and important organ whose functions include not only digestion of food and absorption of nutrients, but also synthesis and storage of macromolecules, initiation of innate immune response to pathogens as well as the production of egg yolk protein -. In C. elegans, the intestine develops from a single cell lineage, beginning with progenitor cell E. During this development, GATA TFs regulate and activate intestine-specific gene expression which supports intestinal differentiation and maintenance ,. Distinctive localization of Ce-riok-3 to larval and adult intestine and its enrichment in hermaphrodites  could indicate that Ce-RIOK-3 supports intestinal development and function, and suggests that RIOK-3 plays a role in the reproductive biology of C. elegans. Similarly, the abundance of Ss-riok-3 transcripts in P Females and FL Females of S. stercoralis as well as the intestinal localization of Ss-riok-3 promoter activity suggest that Ss-RIOK-3 supports intestinal function during development from larvae to adults, and possibly reproductive functions in adult females of this parasitic nematode.
In addition to the intestine of S. stercoralis, the head neuron of PFL L1 - L2s also had GFP expression that is unique to S. stercoralis compared with expression pattern for Ce-riok-3 in C. elegans, indicating a functional difference between Ss-RIOK-3 and Ce-RIOK-3, and could suggest central and distinctive roles for RIOK-3 in the S. stercoralis, which warrant further investigation. Also, the shift of Ss-riok-3 promoter activity from intestine in PFL L1-L2s to body wall muscle in iL3s, which is also found in the promoter activity of Ss-riok-1 from neurons in PFL L1 to body wall muscle in iL3 , suggests a remolding of tissues in iL3 to prepare the parasite for infecting the host animal .
In conclusion, we have identified the RIOK-3 encoding gene Ss-riok-3 in S. stercoralis. Ss-RIOK-3 is predicted to contain conserved RIO domain with two aa substitutions in the ATP-binding motif compared with RIOK-3 s from other nematodes, and from other selected species of invertebrates and vertebrates. Ss-riok-3 transcripts are most abundant in the parasitic stages of S. stercoralis, and the Ss-riok-3 promoter is active in the intestine and some cephalic neurons of PF L1-L2s, suggesting that Ss-riok-3 supports the development of the intestine of larvae and could be involved in the reproductive biology of female adults. The shift of Ss-riok-3 promoter activity during the development of S. stercoralis from PFL L1 to iL3 suggests a potential role of Ss-riok-3 in morphogenesis of iL3. The finding that the TATA boxes located in the 3’-UTR of Ss-rep-1, the gene immediately upstream of Ss-riok-3, are essential regulatory elements to initiate the transcription of Ss-riok-3 also underscores the functional overlap of regulatory sequences in genes that are closely linked in the S. stercoralis genome. Whether Ss-RIOK-3 participates in the reproductive biology in female adults still needs further investigation.
Sincere thanks to Hongguang Shao and Xinshe Li for assistance with gonad injection in S. stercoralis. This study was supported by the Special Fund for Agro-scientific Research in the Public Interest (Grant No. 201303037) and the Fundamental Research Funds for the Central Universities (Program no. 2011PY118) to MH, and by grants from the National Institutes of Health (NIH), USA (AI-050688, AI-082548 and AI-105856) to JBL. Funds from the National Health and Medical Research Council (NHMRC) of Australia and the Australian Research Council (ARC) are gratefully acknowledged (RBG); this study was also supported by a Victorian Life Sciences Computation Initiative (VLSCI) grant number VR0007 on its Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government (RBG). Other support from the Australian Academy of Science and the Alexander von Humboldt Foundation is gratefully acknowledged (RBG).
- Croker C, Reporter R, Redelings M, Mascola L: Strongyloidiasis-related deaths in the United States, 1991-2006. Am J Trop Med Hyg. 2010, 83 (2): 422-426. 10.4269/ajtmh.2010.09-0750.PubMed CentralView ArticlePubMedGoogle Scholar
- Schad G: Morphology and life history of Strongyloides stercoralis. Strongyloidiasis: a Major Roundworm Infection of Man. 1989, Taylor & Francis, Philadelphia, 85-104.Google Scholar
- Hanks SK, Quinn AM, Hunter T: The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science. 1988, 241 (4861): 42-52. 10.1126/science.3291115.View ArticlePubMedGoogle Scholar
- Manning G, Whyte DB, Martinez R, Hunter T, Sudarsanam S: The protein kinase complement of the human genome. Science. 2002, 298 (5600): 1912-1934. 10.1126/science.1075762.View ArticlePubMedGoogle Scholar
- LaRonde-LeBlanc N, Wlodawer A: A family portrait of the RIO kinases. J Biol Chem. 2005, 280 (45): 37297-37300. 10.1074/jbc.R500013200.View ArticlePubMedGoogle Scholar
- LaRonde-LeBlanc N, Guszczynski T, Copeland T, Wlodawer A: Structure and activity of the atypical serine kinase Rio1. FEBS J. 2005, 272 (14): 3698-3713. 10.1111/j.1742-4658.2005.04796.x.View ArticlePubMedGoogle Scholar
- Vanrobays E, Gleizes PE, Bousquet-Antonelli C, Noaillac-Depeyre J, Caizergues-Ferrer M, Gelugne JP: Processing of 20S pre-rRNA to 18S ribosomal RNA in yeast requires Rrp10p, an essential non-ribosomal cytoplasmic protein. EMBO J. 2001, 20 (15): 4204-4213. 10.1093/emboj/20.15.4204.PubMed CentralView ArticlePubMedGoogle Scholar
- Geerlings TH, Faber AW, Bister MD, Vos JC, Raue HA: Rio2p, an evolutionarily conserved, low abundant protein kinase essential for processing of 20 S Pre-rRNA in Saccharomyces cerevisiae. J Biol Chem. 2003, 278 (25): 22537-22545. 10.1074/jbc.M300759200.View ArticlePubMedGoogle Scholar
- Widmann B, Wandrey F, Badertscher L, Wyler E, Pfannstiel J, Zemp I, Kutay U: The kinase activity of human Rio1 is required for final steps of cytoplasmic maturation of 40S subunits. Mol Biol Cell. 2012, 23 (1): 22-35. 10.1091/mbc.E11-07-0639.PubMed CentralView ArticlePubMedGoogle Scholar
- Baumas K, Soudet J, Caizergues-Ferrer M, Faubladier M, Henry Y, Mougin A: Human RioK3 is a novel component of cytoplasmic pre-40S pre-ribosomal particles. RNA Biol. 2012, 9 (2): 162-174. 10.4161/rna.18810.View ArticlePubMedGoogle Scholar
- Roesch A, Vogt T, Stolz W, Dugas M, Landthaler M, Becker B: Discrimination between gene expression patterns in the invasive margin and the tumour core of malignant melanomas. Melanoma Res. 2003, 13 (5): 503-509. 10.1097/00008390-200310000-00009.View ArticlePubMedGoogle Scholar
- Giri U, Ashorn CL, Ramdas L, Stivers DN, Coombes K, El-Naggar AK, Ang KK, Story MD: Molecular signatures associated with clinical outcome in patients with high-risk head-and-neck squamous cell carcinoma treated by surgery and radiation. Int J Radiat Oncol Biol Phys. 2006, 64 (3): 670-677. 10.1016/j.ijrobp.2005.08.032.View ArticlePubMedGoogle Scholar
- Kimmelman AC, Hezel AF, Aguirre AJ, Zheng H, Paik JH, Ying H, Chu GC, Zhang JX, Sahin E, Yeo G, Ponugoti A, Nabioullin R, Deroo S, Yang S, Wang X, McGrath JP, Protopopova M, Ivanova E, Zhang J, Feng B, Tsao MS, Redston M, Protopopov A, Xiao Y, Futreal PA, Hahn WC, Klimstra DS, Chin L, DePinho RA: Genomic alterations link Rho family of GTPases to the highly invasive phenotype of pancreas cancer. Proc Natl Acad Sci U S A. 2008, 105 (49): 19372-19377. 10.1073/pnas.0809966105.PubMed CentralView ArticlePubMedGoogle Scholar
- Shan J, Wang P, Zhou J, Wu D, Shi H, Huo K: RIOK3 interacts with caspase-10 and negatively regulates the NF-kappaB signaling pathway. Mol Cell Biochem. 2009, 332 (1–2): 113-120. 10.1007/s11010-009-0180-8.View ArticlePubMedGoogle Scholar
- Stephens P, Edkins S, Davies H, Greenman C, Cox C, Hunter C, Bignell G, Teague J, Smith R, Stevens C, O'Meara S, Parker A, Tarpey P, Avis T, Barthorpe A, Brackenbury L, Buck G, Butler A, Clements J, Cole J, Dicks E, Edwards K, Forbes S, Gorton M, Gray K, Halliday K, Harrison R, Hills K, Hinton J, Jones D: A screen of the complete protein kinase gene family identifies diverse patterns of somatic mutations in human breast cancer. Nat Genet. 2005, 37 (6): 590-592. 10.1038/ng1571.View ArticlePubMedGoogle Scholar
- Gonczy P, Echeverri C, Oegema K, Coulson A, Jones SJ, Copley RR, Duperon J, Oegema J, Brehm M, Cassin E, Hannak E, Kirkham M, Pichler S, Flohrs K, Goessen A, Leidel S, Alleaume AM, Martin C, Ozlu N, Bork P, Hyman AA: Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III. Nature. 2000, 408 (6810): 331-336. 10.1038/35042526.View ArticlePubMedGoogle Scholar
- Rual JF, Ceron J, Koreth J, Hao T, Nicot AS, Hirozane-Kishikawa T, Vandenhaute J, Orkin SH, Hill DE, van den Heuvel S, Vidal M: Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library. Genome Res. 2004, 14 (10B): 2162-2168. 10.1101/gr.2505604.PubMed CentralView ArticlePubMedGoogle Scholar
- Sonnichsen B, Koski LB, Walsh A, Marschall P, Neumann B, Brehm M, Alleaume AM, Artelt J, Bettencourt P, Cassin E, Hewitson M, Holz C, Khan M, Lazik S, Martin C, Nitzsche B, Ruer M, Stamford J, Winzi M, Heinkel R, Roder M, Finell J, Hantsch H, Jones SJ, Jones M, Piano F, Gunsalus KC, Oegema K, Gonczy P, Coulson A: Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans. Nature. 2005, 434 (7032): 462-469. 10.1038/nature03353.View ArticlePubMedGoogle Scholar
- Geldhof P, Visser A, Clark D, Saunders G, Britton C, Gilleard J, Berriman M, Knox D: RNA interference in parasitic helminths: current situation, potential pitfalls and future prospects. Parasitology. 2007, 134 (Pt 5): 609-619. 10.1017/S0031182006002071.View ArticlePubMedGoogle Scholar
- Castelletto ML, Massey HC, Lok JB: Morphogenesis of Strongyloides stercoralis infective larvae requires the DAF-16 ortholog FKTF-1. PLoS Pathog. 2009, 5 (4): e1000370-10.1371/journal.ppat.1000370.PubMed CentralView ArticlePubMedGoogle Scholar
- Li X, Massey HC, Nolan TJ, Schad GA, Kraus K, Sundaram M, Lok JB: Successful transgenesis of the parasitic nematode Strongyloides stercoralis requires endogenous non-coding control elements. Int J Parasitol. 2006, 36 (6): 671-679. 10.1016/j.ijpara.2005.12.007.View ArticlePubMedGoogle Scholar
- Shao H, Li X, Nolan TJ, Massey HC, Pearce EJ, Lok JB: Transposon-mediated chromosomal integration of transgenes in the parasitic nematode Strongyloides ratti and establishment of stable transgenic lines. PLoS Pathog. 2012, 8 (8): e1002871-10.1371/journal.ppat.1002871.PubMed CentralView ArticlePubMedGoogle Scholar
- Lok JB, Massey HC: Transgene expression in Strongyloides stercoralis following gonadal microinjection of DNA constructs. Mol Biochem Parasitol. 2002, 119 (2): 279-284. 10.1016/S0166-6851(01)00414-5.View ArticlePubMedGoogle Scholar
- Lok JB:Strongyloides stercoralis: a model for translational research on parasitic nematode biology. WormBook. 2007, 1-18.Google Scholar
- Hawdon J, Schad G: Long term storage of hookworm infective larvae in buffered saline solution maintains larval responsiveness to host signals. J Helm Soc Wash. 1991, 58: 140-142.Google Scholar
- Bowman DD, Lynn RC: Georgi's Parasitology for Veterinarians. WB Saunders Company. 1995, W B Saunders Co, Saint Louis, Missouri, U.S.AGoogle Scholar
- Gasser RB, Chilton NB, Hoste H, Beveridge I: Rapid sequencing of rDNA from single worms and eggs of parasitic helminths. Nucleic Acids Res. 1993, 21 (10): 2525-2526. 10.1093/nar/21.10.2525.PubMed CentralView ArticlePubMedGoogle Scholar
- Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25 (17): 3389-3402. 10.1093/nar/25.17.3389.PubMed CentralView ArticlePubMedGoogle Scholar
- Bairoch A: The PROSITE dictionary of sites and patterns in proteins, its current status. Nucleic Acids Res. 1993, 21 (13): 3097-3103. 10.1093/nar/21.13.3097.PubMed CentralView ArticlePubMedGoogle Scholar
- Bateman A, Birney E, Durbin R, Eddy SR, Howe KL, Sonnhammer EL: The Pfam protein families database. Nucleic Acids Res. 2000, 28 (1): 263-266. 10.1093/nar/28.1.263.PubMed CentralView ArticlePubMedGoogle Scholar
- Katoh K, Standley DM: MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol. 2013, 30 (4): 772-780. 10.1093/molbev/mst010.PubMed CentralView ArticlePubMedGoogle Scholar
- Deyneko IV, Kel AE, Kel-Margoulis OV, Deineko EV, Wingender E, Weiss S: MatrixCatch–a novel tool for the recognition of composite regulatory elements in promoters. BMC Bioinformatics. 2013, 14: 241-10.1186/1471-2105-14-241.PubMed CentralView ArticlePubMedGoogle Scholar
- Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S: MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011, 28 (10): 2731-2739. 10.1093/molbev/msr121.PubMed CentralView ArticlePubMedGoogle Scholar
- Stoltzfus JD, Massey HC, Nolan TJ, Griffith SD, Lok JB:Strongyloides stercoralis age-1: a potential regulator of infective larval development in a parasitic nematode. PLoS One. 2012, 7 (6): e38587-10.1371/journal.pone.0038587.PubMed CentralView ArticlePubMedGoogle Scholar
- Stoltzfus JD, Minot S, Berriman M, Nolan TJ, Lok JB: RNAseq analysis of the parasitic nematode Strongyloides stercoralis reveals divergent regulation of canonical dauer pathways. PLoS Negl Trop Dis. 2012, 6 (10): e1854-10.1371/journal.pntd.0001854.PubMed CentralView ArticlePubMedGoogle Scholar
- Nolan TJ, Megyeri Z, Bhopale VM, Schad GA:Strongyloides stercoralis: the first rodent model for uncomplicated and hyperinfective strongyloidiasis, the Mongolian gerbil (Meriones unguiculatus). J Infect Dis. 1993, 168 (6): 1479-1484. 10.1093/infdis/168.6.1479.View ArticlePubMedGoogle Scholar
- Schad GA, Hellman ME, Muncey DW:Strongyloides stercoralis: hyperinfection in immunosuppressed dogs. Exp Parasitol. 1984, 57 (3): 287-296. 10.1016/0014-4894(84)90103-6.View ArticlePubMedGoogle Scholar
- Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B: Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods. 2008, 5 (7): 621-628. 10.1038/nmeth.1226.View ArticlePubMedGoogle Scholar
- Trapnell C, Roberts A, Goff L, Pertea G, Kim D, Kelley DR, Pimentel H, Salzberg SL, Rinn JL, Pachter L: Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. Nat Protoc. 2012, 7 (3): 562-578. 10.1038/nprot.2012.016.PubMed CentralView ArticlePubMedGoogle Scholar
- Trapnell C, Hendrickson DG, Sauvageau M, Goff L, Rinn JL, Pachter L: Differential analysis of gene regulation at transcript resolution with RNA-seq. Nat Biotechnol. 2013, 31 (1): 46-53. 10.1038/nbt.2450.View ArticlePubMedGoogle Scholar
- Junio AB, Li X, Massey HC, Nolan TJ, Todd Lamitina S, Sundaram MV, Lok JB:Strongyloides stercoralis: cell- and tissue-specific transgene expression and co-transformation with vector constructs incorporating a common multifunctional 3' UTR. Exp Parasitol. 2008, 118 (2): 253-265. 10.1016/j.exppara.2007.08.018.PubMed CentralView ArticlePubMedGoogle Scholar
- Yuan W, Lok JB, Stoltzfus JD, Gasser RB, Fang F, Lei WQ, Fang R, Zhou YQ, Zhao JL, Hu M: Toward understanding the functional role of Ss-riok-1, a RIO protein kinase-encoding gene of Strongyloides stercoralis. PLoS Negl Trop Dis. 2014, 8 (8): e3062-10.1371/journal.pntd.0003062.PubMed CentralView ArticlePubMedGoogle Scholar
- Angermayr M, Roidl A, Bandlow W: Yeast Rio1p is the founding member of a novel subfamily of protein serine kinases involved in the control of cell cycle progression. Mol Microbiol. 2002, 44 (2): 309-324. 10.1046/j.1365-2958.2002.02881.x.View ArticlePubMedGoogle Scholar
- Blaxter ML, De Ley P, Garey JR, Liu LX, Scheldeman P, Vierstraete A, Vanfleteren JR, Mackey LY, Dorris M, Frisse LM, Vida JT, Thomas WK: A molecular evolutionary framework for the phylum Nematoda. Nature. 1998, 392 (6671): 71-75. 10.1038/32160.View ArticlePubMedGoogle Scholar
- Campbell BE, Boag PR, Hofmann A, Cantacessi C, Wang CK, Taylor P, Hu M, Sindhu ZU, Loukas A, Sternberg PW, Gasser RB: Atypical (RIO) protein kinases from Haemonchus contortus - promise as new targets for nematocidal drugs. Biotechnol Adv. 2011, 29 (3): 338-350. 10.1016/j.biotechadv.2011.01.006.View ArticlePubMedGoogle Scholar
- Andres DA, Seabra MC, Brown MS, Armstrong SA, Smeland TE, Cremers FP, Goldstein JL: cDNA cloning of component A of Rab geranylgeranyl transferase and demonstration of its role as a Rab escort protein. Cell. 1993, 73 (6): 1091-1099. 10.1016/0092-8674(93)90639-8.View ArticlePubMedGoogle Scholar
- Nonet ML, Staunton JE, Kilgard MP, Fergestad T, Hartwieg E, Horvitz HR, Jorgensen EM, Meyer BJ:Caenorhabditis elegans rab-3 mutant synapses exhibit impaired function and are partially depleted of vesicles. J Neurosci. 1997, 17 (21): 8061-8073.PubMedGoogle Scholar
- Novick P, Zerial M: The diversity of Rab proteins in vesicle transport. Curr Opin Cell Biol. 1997, 9 (4): 496-504. 10.1016/S0955-0674(97)80025-7.View ArticlePubMedGoogle Scholar
- Mahoney TR, Liu Q, Itoh T, Luo S, Hadwiger G, Vincent R, Wang ZW, Fukuda M, Nonet ML: Regulation of synaptic transmission by RAB-3 and RAB-27 in Caenorhabditis elegans. Mol Biol Cell. 2006, 17 (6): 2617-2625. 10.1091/mbc.E05-12-1170.PubMed CentralView ArticlePubMedGoogle Scholar
- Blumenthal T, Steward K: RNA Processing and Gene Structure. In C elegans II. 2nd edition. Edited by Riddle DL, Blumenthal T, Meyer BJ, Priess JR. Cold Spring Harbor (NY): 1997.Google Scholar
- Angermayr M, Bandlow W: The type of basal promoter determines the regulated or constitutive mode of transcription in the common control region of the yeast gene pair GCY1/RIO1. J Biol Chem. 1997, 272 (50): 31630-31635. 10.1074/jbc.272.50.31630.View ArticlePubMedGoogle Scholar
- Vanrobays E, Gelugne JP, Gleizes PE, Caizergues-Ferrer M: Late cytoplasmic maturation of the small ribosomal subunit requires RIO proteins in Saccharomyces cerevisiae. Mol Cell Biol. 2003, 23 (6): 2083-2095. 10.1128/MCB.23.6.2083-2095.2003.PubMed CentralView ArticlePubMedGoogle Scholar
- Fenner BJ, Scannell M, Prehn JH: Identification of polyubiquitin binding proteins involved in NF-kappaB signaling using protein arrays. Biochim Biophys Acta. 2009, 1794 (7): 1010-1016. 10.1016/j.bbapap.2009.02.013.View ArticlePubMedGoogle Scholar
- Feng J, De Jesus PD, Su V, Han S, Gong D, Wu NC, Tian Y, Li X, Wu T-T, Chanda SK: RIOK3 is an adaptor protein required for IRF3-mediated antiviral type I interferon production. J Virol. 2014, JVI: 00614-00643.Google Scholar
- LaRonde-LeBlanc N, Wlodawer A: Crystal structure of A. fulgidus Rio2 defines a new family of serine protein kinases. Structure. 2004, 12 (9): 1585-1594. 10.1016/j.str.2004.06.016.View ArticlePubMedGoogle Scholar
- Ferreira-Cerca S, Sagar V, Schafer T, Diop M, Wesseling AM, Lu H, Chai E, Hurt E, LaRonde-LeBlanc N: ATPase-dependent role of the atypical kinase Rio2 on the evolving pre-40S ribosomal subunit. Nat Struct Mol Biol. 2012, 19 (12): 1316-1323. 10.1038/nsmb.2403.PubMed CentralView ArticlePubMedGoogle Scholar
- Breugelmans B, Jex AR, Korhonen PK, Mangiola S, Young ND, Sternberg PW, Boag PR, Hofmann A, Gasser RB: Bioinformatic exploration of RIO protein kinases of parasitic and free-living nematodes. Int J Parasitol. 2014, 44 (11): 827-836. 10.1016/j.ijpara.2014.06.005.View ArticlePubMedGoogle Scholar
- Levin M, Hashimshony T, Wagner F, Yanai I: Developmental milestones punctuate gene expression in the Caenorhabditis embryo. Dev Cell. 2012, 22 (5): 1101-1108. 10.1016/j.devcel.2012.04.004.View ArticlePubMedGoogle Scholar
- Reinke V, Gil IS, Ward S, Kazmer K: Genome-wide germline-enriched and sex-biased expression profiles in Caenorhabditis elegans. Development. 2004, 131 (2): 311-323. 10.1242/dev.00914.View ArticlePubMedGoogle Scholar
- Blumenthal T, Evans D, Link CD, Guffanti A, Lawson D, Thierry-Mieg J, Thierry-Mieg D, Chiu WL, Duke K, Kiraly M, Kim SK: A global analysis of Caenorhabditis elegans operons. Nature. 2002, 417 (6891): 851-854. 10.1038/nature00831.View ArticlePubMedGoogle Scholar
- Breathnach R, Chambon P: Organization and expression of eucaryotic split genes coding for proteins. Annu Rev Biochem. 1981, 50: 349-383. 10.1146/annurev.bi.50.070181.002025.View ArticlePubMedGoogle Scholar
- Corden J, Wasylyk B, Buchwalder A, Sassone-Corsi P, Kedinger C, Chambon P: Promoter sequences of eukaryotic protein-coding genes. Science. 1980, 209 (4463): 1406-1414. 10.1126/science.6251548.View ArticlePubMedGoogle Scholar
- Patient RK, McGhee JD: The GATA family (vertebrates and invertebrates). Curr Opin Genet Dev. 2002, 12 (4): 416-422. 10.1016/S0959-437X(02)00319-2.View ArticlePubMedGoogle Scholar
- Abel T, Michelson AM, Maniatis T: A Drosophila GATA family member that binds to Adh regulatory sequences is expressed in the developing fat body. Development. 1993, 119 (3): 623-633.PubMedGoogle Scholar
- Arceci RJ, King AA, Simon MC, Orkin SH, Wilson DB: Mouse GATA-4: a retinoic acid-inducible GATA-binding transcription factor expressed in endodermally derived tissues and heart. Mol Cell Biol. 1993, 13 (4): 2235-2246.PubMed CentralView ArticlePubMedGoogle Scholar
- Kelley C, Blumberg H, Zon LI, Evans T: GATA-4 is a novel transcription factor expressed in endocardium of the developing heart. Development. 1993, 118 (3): 817-827.PubMedGoogle Scholar
- Laverriere AC, MacNeill C, Mueller C, Poelmann RE, Burch JB, Evans T: GATA-4/5/6, a subfamily of three transcription factors transcribed in developing heart and gut. J Biol Chem. 1994, 269 (37): 23177-23184.PubMedGoogle Scholar
- Maeda M: Gastric proton pump (H+/K(+)-ATPase): structure and gene regulation through GATA DNA-binding protein(s). J Biochem. 1994, 115 (1): 6-14.PubMedGoogle Scholar
- Britton C, McKerrow JH, Johnstone IL: Regulation of the Caenorhabditis elegans gut cysteine protease gene cpr-1: requirement for GATA motifs. J Mol Biol. 1998, 283 (1): 15-27. 10.1006/jmbi.1998.2093.View ArticlePubMedGoogle Scholar
- McGhee JD, Fukushige T, Krause MW, Minnema SE, Goszczynski B, Gaudet J, Kohara Y, Bossinger O, Zhao Y, Khattra J, Hirst M, Jones SJ, Marra MA, Ruzanov P, Warner A, Zapf R, Moerman DG, Kalb JM: ELT-2 is the predominant transcription factor controlling differentiation and function of the C. elegans intestine, from embryo to adult. Dev Biol. 2009, 327 (2): 551-565. 10.1016/j.ydbio.2008.11.034.PubMed CentralView ArticlePubMedGoogle Scholar
- McKay SJ, Johnsen R, Khattra J, Asano J, Baillie DL, Chan S, Dube N, Fang L, Goszczynski B, Ha E, Halfnight E, Hollebakken R, Huang P, Hung K, Jensen V, Jones SJ, Kai H, Li D, Mah A, Marra M, McGhee J, Newbury R, Pouzyrev A, Riddle DL, Sonnhammer E, Tian H, Tu D, Tyson JR, Vatcher G, Warner A: Gene expression profiling of cells, tissues, and developmental stages of the nematode C. elegans. Cold Spring Harb Symp Quant Biol. 2003, 68: 159-169. 10.1101/sqb.2003.68.159.View ArticlePubMedGoogle Scholar
- Hunt-Newbury R, Viveiros R, Johnsen R, Mah A, Anastas D, Fang L, Halfnight E, Lee D, Lin J, Lorch A, McKay S, Okada HM, Pan J, Schulz AK, Tu D, Wong K, Zhao Z, Alexeyenko A, Burglin T, Sonnhammer E, Schnabel R, Jones SJ, Marra MA, Baillie DL, Moerman DG: High-throughput in vivo analysis of gene expression in Caenorhabditis elegans. PLoS Biol. 2007, 5 (9): e237-10.1371/journal.pbio.0050237.PubMed CentralView ArticlePubMedGoogle Scholar
- Kimble J, Sharrock WJ: Tissue-specific synthesis of yolk proteins in Caenorhabditis elegans. Dev Biol. 1983, 96 (1): 189-196. 10.1016/0012-1606(83)90322-6.View ArticlePubMedGoogle Scholar
- Schulenburg H, Kurz CL, Ewbank JJ: Evolution of the innate immune system: the worm perspective. Immunol Rev. 2004, 198: 36-58. 10.1111/j.0105-2896.2004.0125.x.View ArticlePubMedGoogle Scholar
- Pauli F, Liu Y, Kim YA, Chen PJ, Kim SK: Chromosomal clustering and GATA transcriptional regulation of intestine-expressed genes in C. elegans. Development. 2006, 133 (2): 287-295. 10.1242/dev.02185.View ArticlePubMedGoogle Scholar
- McGhee JD: The C. elegans intestine. WormBook. 2007, 1-36.Google Scholar
- Zhu J, Hill RJ, Heid PJ, Fukuyama M, Sugimoto A, Priess JR, Rothman JH: End-1 encodes an apparent GATA factor that specifies the endoderm precursor in Caenorhabditis elegans embryos. Genes Dev. 1997, 11 (21): 2883-2896. 10.1101/gad.11.21.2883.PubMed CentralView ArticlePubMedGoogle Scholar
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