Modelling the potential of focal screening and treatment as elimination strategy for Plasmodium falciparum malaria in the Peruvian Amazon Region
© Rosas-Aguirre et al.; licensee BioMed Central. 2015
Received: 21 November 2014
Accepted: 21 April 2015
Published: 7 May 2015
Focal screening and treatment (FSAT) of malaria infections has recently been introduced in Peru to overcome the inherent limitations of passive case detection (PCD) and further decrease the malaria burden. Here, we used a relatively straightforward mathematical model to assess the potential of FSAT as elimination strategy for Plasmodium falciparum malaria in the Peruvian Amazon Region.
A baseline model was developed to simulate a scenario with seasonal malaria transmission and the effect of PCD and treatment of symptomatic infections on the P. falciparum malaria transmission in a low endemic area of the Peruvian Amazon. The model was then adjusted to simulate intervention scenarios for predicting the long term additional impact of FSAT on P. falciparum malaria prevalence and incidence. Model parameterization was done using data from a cohort study in a rural Amazonian community as well as published transmission parameters from previous studies in similar areas. The effect of FSAT timing and frequency, using either microscopy or a supposed field PCR, was assessed on both predicted incidence and prevalence rates.
The intervention model indicated that the addition of FSAT to PCD significantly reduced the predicted P. falciparum incidence and prevalence. The strongest reduction was observed when three consecutive FSAT were implemented at the beginning of the low transmission season, and if malaria diagnosis was done with PCR. Repeated interventions for consecutive years (10 years with microscopy or 5 years with PCR), would allow reaching near to zero incidence and prevalence rates.
The addition of FSAT interventions to PCD may enable to reach P. falciparum elimination levels in low endemic areas of the Amazon Region, yet the progression rates to those levels may vary substantially according to the operational criteria used for the intervention.
KeywordsMalaria Plasmodium falciparum Focal Screening and Treatment (FSAT) Modelling Elimination Peru
Despite several decades of intense control efforts, malaria remains an important public health problem in Peru, especially in the Amazon region where most (85%) of the malaria cases occur, mainly due to P. vivax . Increased financial support from international donors, e.g., the Global Fund-PAMAFRO Project (2005–2010), allowed for the scaling-up of comprehensive malaria control strategies such as the use of artemisinin-based combination therapy (ACT) and the distribution of long-lasting insecticidal mosquito nets (LLINs) . During this period, malaria declined drastically in Peru from 87,805 reported clinical cases in 2005 to 29,355 and 23,075 cases in 2010 and 2011, respectively. Since 2011, following the reduction of financial support allocated to malaria control activities, malaria incidence started to increase again with a doubling of clinical cases in 2013, highlighting the potential risk of rapid malaria resurgence in the Amazon Region .
In contrast to the classical statement that the development of acquired malaria immunity requires long-term exposure and intense transmission, recent studies in endemic countries of South America have reported a large proportion of asymptomatic malaria infections [3-12] suggesting that clinical immunity may develop as well in low-transmission settings of the subcontinent. The wider use of molecular diagnostics in epidemiological studies allows for a better knowledge on the extent of these asymptomatic infections, thus uncovering a “hidden” human infectious reservoir likely to contribute in maintaining transmission in endemic areas [9,13]. Indeed, cross-sectional surveys conducted in rural communities surrounding Iquitos city in Peru reported prevalence of asymptomatic parasite carriers ranging from 5 to 14%, mostly detectable only by PCR (sub-microscopic infections) [3-5].
National malaria surveillance in the Amazon Region relies on passive case detection (PCD) with microscopy . Patients presenting with fever or any other symptoms compatible with malaria are systematically tested by microscopy at peripheral health facilities and treated following national guidelines. However, the major limitation of PCD is that asymptomatic individuals do not seek medical care and therefore remain undetected and untreated [4,15], extending the period of parasite carriage . Other described limitations of PCD include the quality of microscopy-based diagnosis , the access to health services with available microscopy , and social and cultural factors that influence treatment seeking behaviour at community level .
Active case detection (ACD) has recently been recommended in low transmission and malaria-elimination settings to overcome the inherent limitations of PCD and target the asymptomatic parasite reservoir . According to the World Health Organization (WHO), ACD is defined as the detection of malaria infections through household visits in population groups that are considered to be at risk. In the Peruvian Amazon, focal screening and treatment (FSAT), has recently been introduced by the National Malaria Control Program (NMCP) to target malaria hotspots and further decrease the malaria burden. Malaria hotspots are well-delimited villages in rural areas or neighborhoods in periurban areas in which the cumulative malaria incidence over the past 3–5 years is the highest. These hotspots represent ~10-20% of the total malaria reporting-localities and account for more than 80% of the total cases in the Region. The intervention consists of systematic finger prick blood sampling of the whole population (100–1000 inhabitants) during the period of highest incidence (usually between February and May) and the treatment of all microscopically positive infections, irrespective of the presence of symptoms . Following the national treatment guidelines, P. falciparum infections are treated with an ACT -mefloquine and artesunate (MQ-AS)-, and P. vivax infections with chloroquine (CQ) and primaquine (PQ) . However, due to the lack of evidence-based guidelines [21,22], the criteria and procedures to implement FSAT interventions in the Amazon Region have often been arbitrarily decided and are subject to annual variations in logistical and financial resources.
Mathematical models of various levels of complexity have been used for understanding malaria transmission dynamics and informing decision-making in malaria control and elimination efforts [23-26]. Here, we used a model to assess the long term impact of FSAT on the P. falciparum malaria transmission intensity (MIT) in a low endemic area of the Peruvian Amazon Region. Retrospective data from a cohort study in a rural community of the Peruvian Amazon were used to estimate infection rates and a time-variant seasonal vector parameter, and then to evaluate the model. Other malaria-transmission parameters were obtained from previously published studies in the Amazon region. Eventually, the model was used to predict the potential reduction in malaria transmission following the addition of FSAT to the ongoing PCD, allowing for a simulation of the effect size according to the diagnostic tools used, as well as the timing and frequency of FSAT interventions.
While a constant fraction (y) of symptomatic infections of all newly infected individuals is routinely detected by PCD and treated with an ACT of known effectiveness (ε) within the treated state (T), the remaining fraction (1-y*ε), including asymptomatic individuals and previously treated individuals with parasitological treatment failure, remains infectious in an untreated state (I) until the “spontaneous cure” of the disease (i.e. clearance of all parasite forms) unless they are actively detected and treated. Once recovering from compartments T and I, individuals return to the susceptible state (S). While recovery rate for treated individuals (1/r 1 ) is the reciprocal of average human infectious period (i.e. length of time gametocytes remain in the bloodstream) with effective treatment, recovery for untreated individuals requires they pass through two sub-states before becoming recovered: those who have not yet (I1) and those who have spontaneously cleared the asexual blood stage parasites (I2). Then, the rate of movement of individuals from I1 to I2 (1/r 2a ), and from I2 to S (1/r 2b ) are the reciprocal of the average time for the spontaneous clearance of asexual parasites from blood (r 2a ), and the remaining average infectious period after the clearance of blood asexual parasites (r 2b ), respectively in untreated individuals.
Regarding the mosquito population (M), the model includes an initial susceptible state (U) for all mosquitoes, becoming infected at a rate determined by the following three parameters: human feeding rate per mosquito (a), fraction of infectious individuals in the human population ((T + I)/N), and the mosquito susceptibility to infection after biting an infectious individual (c). Infected mosquitoes remain in a latent state (L) for a specific time period before developing sporozoites and becoming infectious (V). The estimation of surviving mosquitoes by the end of the latent period requires an adjustment by the factor e -g*n which incorporates a constant mosquito mortality rate (g) and the average mosquito latent period (n). The model assumes that mosquitoes remain infectious until they die.
The main assumptions of this model are the following: i) human and mosquito populations remain constant (number of births and deaths are equal at any time); ii) malaria-attributable deaths are not explicitly incorporated (malaria mortality rate is similar to general mortality rate); iii) no individual differences in transmission parameters between subjects; iv) super-infections do not occur ; v) all infected individuals develop mature gametocytes immediately after they leave the latent sub-state E2; vi) any gametocytemia represents potential for transmission; and vii) there is no prophylactic effect of the drugs post recovery.
Retrospective data on malaria cohort study
The model was fine-tuned by using retrospective data from a cohort study carried out between 2003 and 2008 in four rural communities of San Juan district, south of Iquitos city in the Peruvian Amazon [4,28,29]. Malaria transmission in this area typically starts in January and finishes in July with a peak of clinical malaria cases between February and March; P. vivax and P. falciparum infections occurring at a ratio 4:1. Previous reports from this area indicated that more than two thirds of P. falciparum infections were asymptomatic with low parasite density [3,4]. Anopheles darlingi is the major malaria vector with a strong anthropophilic behavior [30,31]. The initial cross-sectional survey conducted in April 2003 among cohort participants showed that most of them reported sleeping under bed nets, although none of them mentioned the use of insecticide treated nets or repellents in their homes . From 2004 to 2008, biannual community screening campaigns were carried out (beginning-end of each malaria season), and in between, weekly ACD for P. falciparum infections was conducted, sampling each month a new group of individuals living within a 100-m radius of houses where at least one P. falciparum infection was detected during the previous month (index houses), either through the surveys, ACD or the routine PCD at health facilities . Since high coverage during screening campaigns was achieved only in Ninarumi community, local model parameters were estimated from this community only, and taking into account malariometric indices from the year 2004 only, which was the first full year of ACD in the cohort study. The prevalence of P. falciparum infections by microscopy in Ninarumi was measured at 6.5% and 1.1%, respectively in January and August 2004 (Additional file 2: Table S1); and during that year a total of 139 new P. falciparum infections were detected (0.28 infections/person/year), 91 (65.4%) of them being symptomatic either at the time of sampling or during the following week. Of the additional 48 infections which remained asymptomatic during the follow-up, 19 were confirmed only by retrospective PCR analysis on blood samples collected on filter paper from individuals during weekly ACDs. The detailed procedures for the PCR analysis are described elsewhere . All individuals enrolled in the cohort study gave signed informed consent or assent. Ethical clearance was received from New York University (NYU Institutional Review Board [IRB] approval no. 08–982), the University of Alabama at Birmingham, and the Peruvian Ministry of Health and National Institutes of Health Internal Ethical Review Boards .
Transmission parameter values
Baseline model parameters
Mosquito density (ratio female mosquitoes/humans)
Assumed value within published range
Human feeding rate, bites per mosquito per day
0.035 - 0.32
Monthly estimations from fitting cohort data; values within published range
Susceptibility of an individual to infection after being bitten by an infectious mosquito
Estimation using cohort and published data
Mosquito susceptibility to infection after biting an infectious individual
Duration of human latent period for Pf* in d]’ cays (l = l 1 + l 2 )
Duration from sporozoite inoculation to development of blood asexual parasites in days
Duration from asexual blood stage infection to development of gametocytes in days
Duration of mosquito latent period in days
Daily human mortality rate
Daily mosquito mortality rate
Fraction of symptomatic infections
Duration of infectious period in treated humans with ACT (in days)
Duration of infectious period in untreated humans in days (r 2 = r 2a + r 2b )
Duration of presence of blood asexual parasites in untreated humans in days
Duration of infectious period in untreated humans after clearance of blood asexual parasites in days
Coverage of FSAT
Duration of FSAT in days
Sensitivity of microscopy for detecting Pf* asexual stages
Sensitivity of microscopy for detecting Pf*gametocytes
Sensitivity of highly sensitive diagnostic tests for detecting asexual stages
Sensitivity of highly sensitive diagnostic tests for detecting gametocytes
FSAT model and assessment of interventions
In the scenario with FSAT interventions, the baseline model was improved by allowing movements from sub-states E2, I1 and I2 to the treated state (T) (red dotted lines in Figure 1), at specific rates that are determined by the coverage (∆), the reciprocal of duration of the intervention (1/v), the sensitivity of the diagnostic test used for malaria screening (s), and the effectiveness of treatment in confirmed infections (ε).
After setting initial conditions for human and mosquito compartments based on the average observed prevalence in the 2004 Ninarumi surveys and the reported A. darlingi sporozoite rates in the area , the baseline model was run until reaching steady levels (baseline scenario), indicated by the stable cumulative number of P. falciparum infections recorded for 20 more simulated years. Then, the model was run for an additional year (year 0) after which interventions based on FSAT (intervention scenario) were added to the routine PCD (baseline scenario). A first intervention scenario was modeled with the following assumptions: one FSAT during the peak of the high transmission season (only in February of year 1); FSAT covers 100% of population (∆ = 1) in one day (v = 1); microscopy used in FSAT allows for the detection of 50% of asexual blood-stage infections by P. falciparum (sub-states E2 and I1), and 10% of infections with only sexual stages (gametocytes) in blood (sub-state I2) ; and all detected P. falciparum infections are treated with a 98% effectiveness (ε = 0.98).
Additional intervention scenarios were modeled to assess the impact of different operational criteria for FSAT implemented during only one year (i.e. timing, number and time intervals between consecutive interventions) on the predicted cumulative incidence and prevalence of P. falciparum infections. Besides microscopy, a field PCR was also considered as diagnostic test during malaria screening, assuming sensitivities of 95%, 95% and 80% for detecting infections in sub-states E2, I1, and I2, respectively. The predicted cumulative incidence (i.e. cumulative number of new symptomatic and asymptomatic infections) was recorded over one-year and five-year periods from the start of the first FSAT in each intervention scenario, and was compared with the cumulative incidence found with the baseline PCD scenario. The predicted impact of each intervention was determined as the percentage difference between the cumulative number of new P. falciparum infections with baseline and with the respective intervention scenarios (cumulative number of infections averted). In addition, minimum and maximum predicted prevalence were determined during both the first and the fifth year after the onset of FSAT interventions. Finally, operational criteria for implementing FSAT interventions during only one year which had the strongest impact were used again to assess the effect of the same interventions if implemented during several consecutive years.
Malaria transmission scenarios were simulated in R software version 2.15.3 (function “dede” in deSolve package) allowing to solve delay differential equations to take into account latent periods for infected individuals and mosquitoes before being infectious (see R code for baseline model in Additional file 4: Text S2) . Baseline model performance was examined by comparing predicted and observed P. falciparum incidence and prevalence from the cohort study. A Wilcoxon matched-pairs signed-ranks test was used to test differences between observed and predicted monthly incidence, with p < 0.05 indicating statistical significance. Different initial values for the A. darlingi sporozoite rate (V/M) were tested and did not affect the results. Moreover, a one-at-a-time sensitivity analysis was conducted to determine the effect of the following parameters on the baseline model predictions : the mosquito density (m), the human feeding rate (a), the fraction of symptomatic infections (y), and the treatment effectiveness (ε). Changes (increase and decrease) of 20% in parameter values were considered in the analysis. Finally, only human parameters (y, ε) were used later to assess their impact on the predictions of the intervention scenarios with FSAT.
The strongest decrease in P. falciparum malaria incidence for interventions implemented during only one year was observed with three consecutive FSAT starting at the beginning of LTS and separated by time intervals of 7 to 60 days (Additional file 5: Table S2), resulting in reductions of more than 40% and 30%, respectively after 1 and 5 years when using microscopy, and of more than 80% and 75%, respectively when using PCR. The impact of the number of consecutive FSAT at the start of LTS (one to three FSAT during only one year, separated by intervals of 30 days) on the predicted P. falciparum prevalence is shown in the Figure 4B. While three consecutive FSATs using PCR would have the largest effect on the predicted prevalence, one FSAT alone using PCR would result in a similar reduction compared to three consecutive FSATs using microscopy. In all simulations including PCR, annual P. falciparum incidence and prevalence would return slowly to the initial levels after 10 years, if FSAT interventions are implemented only during one year.
Using a relatively straightforward mathematical model, our analysis showed that targeting the asymptomatic parasite reservoir with FSAT interventions, in addition to routine PCD, would significantly reduce P. falciparum malaria in low transmission areas of the Peruvian Amazon. The strongest reduction was observed if three consecutive FSAT were implemented annually at the beginning of low transmission season, and if malaria diagnosis was done with a highly sensitive field PCR.
Modelling the effects of interventions that target the asymptomatic malaria reservoir requires a good knowledge of the burden of asymptomatic infections and their transmission dynamics. Cross-sectional surveys in the Peruvian Amazon reported that more than two thirds of malaria infections were asymptomatic [3,4]; however these studies did not take into account the incubation period for some of them, hence the potential development of symptoms at a later stage . Therefore data from the cohort study conducting in peri-Iquitos communities including weekly monitoring of symptoms for one month of all infected individuals, enabled to overcome the limitations of previous survey results. Indeed, an important proportion of P. falciparum infections which were asymptomatic at the time of detection (either by microscopy or by retrospective PCR) developed malaria symptoms (reported and/or measured fever) after one week of follow-up , resulting in only 35% of asymptomatic infections among all confirmed cases in the Ninarumi community in 2004. Another study conducted in the Brazilian Amazon in 2004–2005 found similar results after combining PCD and ACD over a 14-month period, i.e. a total of 326 malaria infections were identified of which 96 (29.4%) were asymptomatic– a much lower proportion than reported in any of the cross-sectional surveys carried out during the same period .
The cohort study also allowed for an accurate measurement of seasonal variations in the incidence of symptomatic and asymptomatic P. falciparum infections in the area, and these seasonal dynamics were well reproduced by the baseline model. In addition, the model predicted not only a 2–3 times higher prevalence of infections during the peak of HTS than during the LTS, but also temporal differences in the proportion of asymptomatic infections, which accounted for about 60% of all prevalent infections during the peak of HTS while they represented almost all infections in the middle of LTS. Moreover, the proportion of prevalent infections detectable by microscopy (“patent infections”) varied also with season, i.e. from almost 50% (6.2% of a total prevalence of 12.3%) in the HTS to 20% (0.7% of a total prevalence of 3.4%) in the LTS. Those predictions suggest that in areas of low and seasonal transmission such as the Peruvian Amazon, non-detectable asymptomatic P. falciparum infections are able to persist during the LTS being potentially infectious to mosquitoes emerging after the start of the rains, as previously described in other areas with markedly seasonal transmission [36,37].
Since symptomatic P. falciparum infections are more likely to be identified and treated during PCD, and the duration of treated infections is considerably shorter than untreated infections; it is anticipated that potential changes in the fraction of symptomatic infections as well as in the effectiveness of case management, may significantly impact the malaria prevalence as shown by the sensitivity analysis. Indeed, simulations showed that a decrease in the fraction of symptomatic infections from 0.65 to 0.5 or a decrease in the treatment effectiveness from 0.98 to 0.8 resulted in similar effects as changes in entomological parameters (ratio female mosquitoes to humans from 75 to 90, human feeding rate by 20%) which are known as being important determinants for the vectorial capacity and the malaria transmission . Asymptomatic infections are becoming increasingly common at low transmission levels [3-12,21], enlarging the human infectious reservoir which will enable onward transmission. On the other hand, treatment effectiveness can decrease due to the spread of drug-resistant strains of P. falciparum parasites and the lack of systems that assure the quality of both the antimalarial drugs and the case management procedures (e.g. poor prescribing practices by health workers, low patient adherence rates to treatment regimens) [22,39].
In contrast to the current strategy of the National Malaria Control Program consisting of adding FSAT with microscopy to PCD during HTS in prioritized localities of the Peruvian Amazon , our simulations showed that conducting a single FSAT at the beginning of LTS would not only result in a greater reduction in P. falciparum incidence and prevalence in the short term, but also decrease the progression rates of these indicators to the initial levels. Furthermore, the effect of FSAT would be maximal if three successive FSAT at time intervals of 7–60 days, would be implemented at the start of the LTS. Kern et al. found a similar impact on the P. falciparum transmission with a single FSAT with microscopy during the dry season using a seasonally-forced malaria model which incorporated periodically mosquito birth rates through a sinusoidal function and different levels of infectiousness for gametocyte carriers; however, while in areas of simulated low disease burden (entomological inoculation rates (EIR) < 10 infective bites/person/year) such as the Peruvian Amazon, the effect was sustained for over three years after the intervention, in areas of high endemicity (EIR > 200) the incidence rates of infection returned to their initial level in the subsequent year . Moreover, the study also indicated that three FSAT scheduled at monthly intervals starting in the dry season had the greatest positive impact on transmission. Successive FSATs would not only allow for identifying infected individuals who were in a latent state at the time of the previous FSAT, but also would increase the coverage of the intervention in real-life situations by including individuals who were absent or not eligible (illness, pregnancy) during the previous FSAT. The relatively wide time interval (i.e. 7–60 days) for a subsequent intervention suggested by our model would facilitate the planning and logistics of mobile teams in charge of FSAT in the targeted communities.
Beside timing and frequency of a given intervention, the other important determinant of impact is the duration (number of years) the intervention needs to be applied to reach sustainable elimination levels. Our model suggested that three successive FSAT with microscopy for ten consecutive years, would result in a sustained declining P. falciparum prevalence and incidence and eventually reach elimination levels. Long-term effects of FSAT has been previously described by Griffin et al. using a complex modelling approach (including immunity, super-infections, and heterogeneity in transmission intensity) fed with published transmission parameters and local epidemiological data of different endemic areas of Africa . The results showed that in low-to-moderate transmission settings, 80% coverage of annual single round of FSAT using rapid diagnostic tests (RDTs) over 15 years, together with 80% coverage of insecticide treated nets (ITNs), would reduce the absolute parasite prevalence by 5–12%. On the other hand, in high transmission areas, the simulated impact of FSAT alone was marginal, the ultimate outcomes strongly depending on the initial transmission level, the frequency and duration of the intervention and the coverage of vector control (i.e. either ITN or indoor residual spraying) .
As a consequence of increased use of molecular tests for detecting submicroscopic malaria infections in both low and high-malaria transmission settings, as well as of recent studies reporting failures of FSAT to reduce transmission when using microscopy or RDTs , it has been suggested that PCR based strategies need to be deployed if such interventions are to be successful [43,44]. Our model simulations assessed the impact of FSAT interventions using an assumed field PCR with high sensitivity, under the assumption that any level of gametocyte density was potentially infectious . Repeated FSAT interventions with PCR for several consecutive years would not only have a stronger impact on P. falciparum malaria transmission (halving time to reach elimination levels), but would also enable to sustain the impact despite eventual reductions in the proportion of symptomatic infections, treatment effectiveness and/or coverage of FSAT. Although these results support the importance of detecting and treating sub-microscopic infections at point of care for successful elimination strategies [22,46], implementing FSAT using PCR as routine strategy in prioritized localities of the Peruvian Amazon may not be feasible since current PCR tests are resource-intensive, time-consuming and expensive . Recently, some studies have shown high sensitivity of non-conventional molecular tests based on loop-mediated isothermal amplification (LAMP) with no requirement for specialist staff and expensive equipment ; these molecular tests need to be optimized quickly to be used in the field for species-specific identification of Plasmodium parasites.
Like with other mathematical models for malaria transmission, our predictions should be interpreted taking into account the underlying assumptions. Some assumptions were intended to simplify the complex malaria dynamics (e.g. constant mosquito and human populations, no individual differences in transmission parameters between subjects), while others attempted to fill the knowledge gap in the P. falciparum malaria transmission cycle (e.g. the development of mature gametocytes in all infected individuals, potential for transmission of any infectious individual independently of its gametocyte density in blood). Further research is still needed to define to what extend asymptomatic and submicroscopic malaria infections contribute to the infectious reservoir ; this information would considerably improve our model. In addition, after the development of a more complex model for P. vivax transmission using data of a recent cohort carried in the Peruvian Amazon , it will help to further fine-tune the model and define operational criteria for FSAT interventions to maximize the impact of the intervention on both species.
Our model suggested that FSAT combined to PCD enables to reach elimination levels in an area of low transmission of the Amazon Region, but the progression rates to those levels vary substantially according to the operational criteria used for the intervention (i.e., timing, number, coverage, and duration of FSAT) and the sensitivity of the diagnostic test used for malaria screening. Despite its limitations, the modelling approach can contribute to design new field studies in the Peruvian Amazon to assess the effectiveness of FSAT and other strategies to further decrease malaria transmission. Even before easy-to-use molecular tests will be available at the point of care, as well as reliable evidence of the effectiveness of FSAT, the current strategy applied by the NMCP in Peru should be revised in the light of our results in order to be more cost effective. Programmatic FSAT interventions with microscopy (minimum of two successive FSAT) can be conducted at the beginning of the LTS in areas with known high risk of P. falciparum malaria transmission, trying to reach the maximum population coverage (avoiding areas with highly mobile populations). Alternatively to reach the areas of mobile populations, resources could be invested to implement a “smart surveillance system” based on reactive case detection guided by information from the PCD.
The study was funded by the Directorate General for Development Cooperation (DGCD) of the Belgian Government within the Third Framework Agreement of the Institutional Collaboration between the Institute of Tropical Medicine “Alexander von Humboldt” - Universidad Peruana Cayetano Heredia, Lima and the Institute of Tropical Medicine in Antwerp, Belgium (FA3-II project, 2011–2013)). Data of a cohort study in a rural community of the Peruvian Amazon were collected as part of the Malaria Immunology and Genetics project (MIGIA) which was funded by National Institute of Allergy and Infectious Diseases-National Institutes of Health (NIAID-NIH, R01 AI64831).
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