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Revealing Annexin A2 and ARF-6 enrollment during Trypanosoma cruzi extracellular amastigote-host cell interaction
Parasites & Vectors volume 8, Article number: 493 (2015)
Invasion of host cells by Trypanosoma cruzi extracellular amastigotes is host actin polymerization-dependent. However, the role of proteins related to actin dynamics during invasion by amastigotes remains to be investigated. Here we describe the role of Annexin A2 and ARF-6 during extracellular amastigote-mammalian cell interactions.
Our results showed ARF-6 accumulation in the amastigote-containing parasitophorous vacuole containing amastigote forms; demonstrated ARF-6 and Annexin A2 critical impact over parasite cell invasion and revealed the effect of Annexin A2 expression on intracellular parasite multiplication.
ARF-6 and Annexin A2 are involved in invasion of mammalian cells by T. cruzi amastigotes.
The Annexin family proteins are implicated in a wide range of cellular responses triggered by increased cytoplasmatic calcium levels . Annexin A2 has an essential role in actin-based macropinocytic rocketing  and is expressed at the interface between F-actin and membranes enriched in phosphatidylinositol 4,5,-biphosphate (PIP2) . Moreover, Annexin A2 is recruited to membrane structures enriched in F-actin during enterophatogenic Escherichia coli host cell adhesion .
The ARF family of small GTPases regulates membrane trafficking and actin cytoskeleton rearrangements . ADP-ribosylation factor 6 (ARF-6) participates during Chlamydia caviae and Yersinia pseudotuberculosis host cell invasion by acting on actin cytoskeleton polymerization [6–8]. In this context, ARF-6 showed an important role during Toxoplasma gondii cell invasion activating PI3-kinase signaling pathway and mobilizing PIP2 and PIP3 to the parasite parasitophorous vacuole .
Invasion of host cells by Trypanosoma cruzi extracellular amastigotes (EA) is host actin polymerization-dependent. EAs induce the formation of crater or cup-like structures enriched in F-actin reliant on host cell type . Here we aimed to study the role of actin polymerization-related proteins, Annexin A2 and ARF-6, during EA host cell interaction in vitro.
Annexin A2 knockout cells displayed an important reduction in EA cell invasion. In contrast, lack of Annexin A2 expression favored EA intracellular multiplication (Fig. 1a and b). Furthermore, ARF-6 knock-down fibroblasts also showed significantly lower number of internalized parasites compared to control cells (Fig. 2a and b). Also, our results demonstrated the accumulation of ARF-6 (Fig. 2c) around EA parasitophorous vacuole. Unfortunately, due to the high recovery dynamics of ARF-6 turnover, we could not evaluate the impact of ARF-6 reduced expression over parasite intracellular multiplication using siRNA treatment.
Although not a common property within the annexin family, several annexins have been shown to interact directly with polymerized actin in vitro, which correlates with the functions that have been proposed for these annexins in mediating, stabilizing and/or regulating membrane–actin interactions . Annexin A2, both as a monomer or in the heterotetrameric complex with S100A10, was the first annexin shown to be capable of binding to and also bundling actin filaments in a Ca2+-dependent manner . Despite its ability to bundle actin filaments, annexin A2 is not found to be associated with prominent actin bundles or cables within cells, such as microvilli or stress fibers. However, more dynamic actin structures, in particular those associated with cellular membranes during phagocytosis, pinocytosis and cell migration contain annexin 2 . Annexin A2 participates in lipid raft formation  and is a PIP2-interacting protein, thereby explaining its affinity to lipid membranes . Taken together, it has been shown that T. cruzi EAs induce host cell calcium signaling  and mobilize lipid raft domains during host cell invasion .
Authors have shown that Annexin A2 functions as a platform for actin remodeling in the vicinity of dynamic cellular membranes . In this context, lack of Annexin A2 expression may have disorganized the actin cytoskeleton which allowed higher parasite multiplication in knockout cells compared to the wild-type ones. Accordingly, Mott et al. (2009)  suggested a physical picture in which an intact, stiff, and rapidly remodeling cytoskeleton facilitates early stages of T. cruzi invasion and parasite retention, followed by subsequent softening and disassembly of the cytoskeleton to accommodate intracellular replication of parasites. These results pointed to actin cytoskeleton as a potential target for novel therapies in order to restrain parasite multiplication and disease progression.
ARF-6 regulates membrane trafficking and interactions of actin cytoskeleton with the plasma membrane. It is involved in membrane trafficking during receptor-mediated endocytosis, endosomal recycling and exocytosis of secretory granules. It is also implicated in the formation of actin-rich membrane protrusions and ruffles. Trafficking of rafts seems to be a major regulatory pathway by which ARF-6 controls Rac1 activation and cell spreading. Induction of PIP2-enriched ruffles and PIP2-positive actin-coated vacuoles by ARF-6 leads to a concomitant accumulation of the annexin 2-p11(S100A10) complex . In this sense, we speculate that during EA-host cell interaction Annexin A2 and ARF-6 may act in a convergent manner.
In summary, our findings demonstrated that ARF-6 and Annexin A2 are involved in the invasion of mammalian cells by T. cruzi amastigotes. Also, ARF-6 was recruited to the amastigote-containing parasitophorous vacuole-containing amastigote. We postulated that ARF-6 and Annexin A2 participate in the reorganization of the actin cytoskeleton during amastigote cell invasion but the exact mechanism remains to be elucidated.
Hayes MJ, Merrifield CJ, Shao D, Ayala-Sanmartin J, Schorey CD, Levine TP, et al. Annexin 2 binding to phosphatidylinositol 4,5-bisphosphate on endocytic vesicles is regulated by the stress response pathway. J Biol Chem. 2004;279:14157–64.
Merrifield CJ, Rescher U, Almers W, Proust J, Gerke V, Sechi AS, et al. Annexin 2 has an essential role in actin-based macropinocytic rocketing. Curr Biol. 2001;11:1136–41.
Hayes MJ, Shao DM, Grieve A, Levine T, Bailly M, Moss SE. Annexin A2 at the interface between F-actin and membranes enriched in phosphatidylinositol 4,5,-biphosphate. Biochim Biophys Acta. 2009;1793:1086–95.
Zobiack N, Rescher U, Laarmann S, Michgehl S, Schmidt MA, Gerke V. Cell-surface attachment of pedestal-forming enteropathogenic E. coli induces a clustering of raft components and a recruitment of annexin 2. J Cell Sci. 2002;115:91–8.
Donaldson JG. Multiple roles for Arf6: sorting, structuring, and signaling at the plasma membrane. J Biol Chem. 2003;278:41573–6.
Niedergang F, Colucci-Guyon E, Dubois T, Raposo G, Chavrier P. ADP ribosylation factor 6 is activated and controls membrane delivery during phagocytosis in macrophages. J Cell Biol. 2003;161:1143–50.
Wong KW, Isberg RR. Arf6 and phosphoinositol-4-phosphate-5-kinase activities permit bypass of the Rac1 requirement for beta1 integrin-mediated bacterial uptake. J Exp Med. 2003;198:603–14.
Blañá ME, Niedergang F, Subtil A, Alcover A, Chavrier P, Dautry-Varsat A. ARF6 GTPase controls bacterial invasion by actin remodeling. J Cell Sci. 2005;118:2201–10.
da Silva CV, da Silva EA, Cruz MC, Chavrier P, Mortara RA. ARF6, PI3-kinase and host cell actin cytoskeleton in Toxoplasma gondii cell invasion. Biochem Biophys Res Commun. 2009;378:656–61. doi:10.1016/j.bbrc.2008.11.108.
Mortara RA, Andreoli WK, Taniwaki NN, Fernandes AB, Silva CV, Fernandes MC, et al. Mammalian cell invasion and intracellular trafficking by Trypanosoma cruzi infective forms. An Acad Bras Cienc. 2005;77:77–94.
Hayes MJ, Rescher U, Gerke V, Moss SE. Annexin-actin interactions. Traffic. 2004;5:571–6.
Lajoie P, Goetz JG, Dennis JW, Nabi IR. Lattices, rafts, and scaffolds: domain regulation of receptor signaling at the plasma membrane. J Cell Biol. 2009;185:381–5.
Rescher U, Ruhe D, Ludwig C, Zobiack N, Gerke V. Annexin 2 is a phosphatidylinositol (4,5)-bisphosphate binding protein recruited to actin assembly sites at cellular membranes. J Cell Sci. 2004;117:3473–80.
Fernandes AB, Neira IT, Ferreira AT, Mortara RA. Cell invasion by Trypanosoma cruzi amastigotes of distinct infectivities: studies on signaling pathways. Parasitol Res. 2006;100:59–68.
Fernandes MC, Cortez M, Geraldo Yoneyama KA, Straus AH, Yoshida N, Mortara RA. Novel strategy in Trypanosoma cruzi cell invasion: implication of cholesterol and host cell microdomains. Int J Parasitol. 2007;37:1431–41.
Grieve AG, Moss SE, Hayes MJ. Annexin A2 at the interface of actin and membrane dynamics: A focus on its roles in endocytosis and cell polarization. Int J Cell Biol. 2012;2012:852430. http://dx.doi.org/10.1155/2012/852430.
Mott A, Lenormand G, Costales J, Fredberg JJ, Burleigh BA. Modulation of host cell mechanics by Trypanosoma cruzi. J Cell Physiol. 2009;218:315–22.
This study was supported by grants and fellowships from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Process: 23038.005295/2011-40) and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Processes: APQ-00305-12; APQ-00913-13).
The authors declare that they have no competing interests exist.
TLT and LC: Performed experiments, analyzed data and helped draft the manuscript. RAM: analyzed data and helped draft the manuscript. CVS: analyzed data, supervised experiments, secured grants, helped draft the manuscript. All authors read and approved the final manuscript.
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Teixeira, T.L., Cruz, L., Mortara, R.A. et al. Revealing Annexin A2 and ARF-6 enrollment during Trypanosoma cruzi extracellular amastigote-host cell interaction. Parasites Vectors 8, 493 (2015). https://doi.org/10.1186/s13071-015-1097-6
- Trypanosoma cruzi
- Extracellular amastigotes
- Annexin A2
- Cell invasion
- Actin cytoskeleton