Transmembrane protein 147 (TMEM147): another partner protein of Haemonchus contortus galectin on the goat peripheral blood mononuclear cells (PBMC)
- Yan Li†1,
- Cheng Yuan†1,
- LiKun Wang1,
- MingMin Lu1,
- YuJian Wang1,
- YuLing Wen1,
- RuoFeng Yan1,
- LiXin Xu1,
- XiaoKai Song1 and
- XiangRui Li1Email author
© The Author(s). 2016
Received: 18 January 2016
Accepted: 13 June 2016
Published: 23 June 2016
Recombinant galectins of male and female Haemonchus contortus (rHco-gal-m/f) have been recognized as significant regulators of the functions of goat peripheral blood mononuclear cells (PBMC). In previous research, transmembrane protein 63A (TMEM63A) was identified as a partner protein in the regulation associated with H. contortus infection. However, in the identification of binding partners for galectins of male and female H. contortus (Hco-gal-m/f) by yeast two-hybrid (YTH) screening, it was found that the transmembrane protein 147 (TMEM147) could also bind to Hco-gal-m/f. In this study, the functions of TMEM147 in the regulations of H. contortus galectin on the goat PBMC were investigated.
To identify Hco-gal-m/f-interacting proteins, a yeast two-hybrid system to detect interactions was used. Co-immunoprecipitation and immunoblotting were used to validate the interaction between recombinant galectins of male H. contortus (rHco-gal-m) and candidate binding protein. The localization of TMEM147 in PBMC was explored by immunofluorescence in confocal imaging studies. Flow cytometry was used to determine the distribution of TMEM147 in T cells, B cells and monocytes in PBMC. The modulatory effects of rHco-gal-m and TMEM147 on cell proliferation, phagocytosis, nitric oxide production, migration, apoptosis and cytokine mRNA transcription were observed by co-incubation of rHco-gal-m and knockdown of the tmem147 gene.
In this research, it was demonstrated that TMEM147 could bind to rHco-gal-m/f. Immunofluorescence assays showed that TMEM147 was localized to the cell membrane and within the cell membrane in goat PBMC. Flow cytometric analysis revealed that TMEM147 was expressed in all B cells and monocytes in goat PBMC. However, 3.8 % of T cells did not express this protein. Knockdown of the tmem147 gene using RNA interference (RNAi) showed that the interaction of galectin with TMEM147 mainly mediated cell proliferation, cell apoptosis, transcription of interleukin-10 (IL-10) and transforming growth factor-β1 (TGF-β1) of goat PBMC. This membrane protein, together with TMEM63A, was also related to the regulation of galectin on phagocytosis and nitric oxide production of goat PBMC. However, it might not be involved in the regulation of galectin on the migration and interferon-γ (IFN-γ) transcription of goat PBMC.
Our results showed that TMEM147 was a binding partner of Hco-gal-m/f and mediated the immunological regulation of Hco-gal-m/f on goat PBMC in a manner different to that of TMEM63A.
Haemonchus contortus is a gastrointestinal parasitic nematode in ruminants (notably goats and sheep) that feeds on blood in the abomasum . Infections with H. contortus can lead to anaemia, weight loss and death, and causes substantial economic losses to livestock production worldwide . Galectins are an evolutionarily ancient family of proteins, closely related to carbohydrate-binding proteins, and located either intracellularly or extracellularly [3–6]. To date, 15 mammalian galectins (galectin-1 to 15) have been cloned and functionally characterized . They can function as important immunological mediators of homeostasis and disease regulation, and display a remarkable functional diversity by participating in the regulation of cell differentiation, proliferation, migration, activation, apoptosis and cytokine production [4–6, 8].
Galectins can be produced by both the parasite and the host. Subsequent studies suggested that parasite galectin might play an important role in host-parasite interactions . It was demonstrated that galectin-1 might mediate Trichomonas vaginalis adherence to human cervical epithelial cells by binding to T. vaginalis lipophosphoglycan (LPG) , and modulate macrophage apoptosis during infection with Trypanosoma cruzi . Galectin-3 was reported to be involved in biological processes that affected the replication of Plasmodium yoelii [12, 13]. Furthermore, galectin-3 could significantly alter the pathogenic course of Toxoplasma gondii , Trypanosoma cruzi  and Leishmania major . In additional to galectin-1 and galectin-3, other galectins are also involved in host-parasite interactions. For example, galectin-9 could recognize L. major by binding to the L. major-specific polygalactosyl epitope , and negatively regulate helper T cell 2-mediated (Th2-mediated) eosinophilic lung inflammation during infection with Ascaris suum . Alternatively, transcription and expression of galectin-11 was increased in the abomasal mucosa following Ostertagia ostertagi infection . Galectin-14 was identified in the mucosal wash from ovine abomasum following Teladorsagia circumcincta larval challenge , and was significantly negatively correlated with worm burden following a challenge infection with H. contortus third-stage larvae (L3s) .
In addition to the above functions, galectins from mammals could also bind to appropriate receptors on the cell surface and participate in a number of biological processes. It has been reported that binding of galectin-3 to mucin 1 (MUC1) could promote tumor cell malignancy  and the cell surface interaction of annexin A2 and galectin-3 could also modulate epidermal growth factor receptor signaling in epidermal growth factor receptor-2 (Her-2) negative breast cancer cells . However, there are few reports regarding the binding partners of these galectins in the host. Therefore, research into the molecular mechanisms that govern the interactions between these galectins and host molecules will shed a new light on galectin-mediated immunomodulation.
In previous research, galectins of male H. contortus (Hco-gal-m) (Acc. No. AY253330) and galectins of female H. contortus (Hco-gal-f) (Acc. No. AY253331) were demonstrated to be two isoforms of galectin derived from male and female H. contortus, respectively . It has also been suggested that recombinant Hco-gal-m/f (rHco-gal-m/f) could bind to the surface of goat PBMC in vitro, and induce biological effects, including promoting cell apoptosis and altering the transcription of interleukin-1β (IL-1β), interleukin-4 (IL-4), IL-10, TGF-β1, IFN-γ and tumor necrosis factor-α (TNF-α) mRNA [25–27]. Furthermore, transcriptional and proteomic analyses revealed that rHco-gal-m/f could modulate several signaling cascades in vitro, including the activation of vascular endothelial growth factor pathway, free radical producing pathway, nuclear factor-kappa B (NFkB) pathway and the ubiquitin-proteasome pathway .
In our previous study, TMEM63A of goat PBMC was identified to be a receptor of Hco-gal-m/f and knockdown of the tmem63a gene by RNAi altered cell proliferation, phagocytosis, nitric oxide production and cytokine mRNA transcription of goat PBMC induced by Hco-gal-m/f . However, in the identification of binding partners for Hco-gal-m and Hco-gal-f by yeast two-hybrid (YTH) screening, it was found that TMEM147 and TMEM63A simultaneously bound to Hco-gal-m/f. However, the functions of TMEM147 in the regulation of Hco-gal-m/f on the PBMC remain unclear and whether TMEM147 is another receptor or partner protein of Hco-gal-m/f on goat PBMC is also unknown. In this study, the functions of TMEM147 in the regulation of H. contortus galectin on the goat PBMC were observed and the results showed that TMEM147 was another partner protein of Hco-gal-m/f. It mediated the functions of goat PBMC induced by Hco-gal-m/f differently with that of TMEM63A.
Animals and cells
Local crossbred goats (3–6 month-old) from the teaching and research flock at Nanjing Agricultural University were housed indoors in pens. They were fed with hay and whole shelled corn and provided with water ad libitum. All goats were dewormed twice at 2 weekly intervals with levamisole (8 mg/kg bodyweight) orally at the time of housing to remove naturally acquired strongylid infections . According to standard parasitological techniques, after two weeks, the helminth eggs in fecal samples from each goat were detected by microscopy. Goats evincing no eggs were used in the subsequent study and the health was observed daily throughout the experiment. The isolation and culture of goat PBMCs and monocytes were performed as previously described .
Identification of binding partners for Hco-gal-m and Hco-gal-f by yeast two-hybrid (YTH) screening
The identification of binding partners for Hco-gal-m/f was performed as previously described using YTH screening .
Validation of the interaction between rHco-gal-m and TMEM147 by co-immunoprecipitation (co-IP) and immunoblotting
To validate the interaction between rHco-gal-m and candidate binding proteins, the forward IP and reverse IP experiments were performed independently as previously described . The goat PBMC stimulated with rHco-gal-m for 12 h were pelleted and lysed. After preclearing treatment, triplicate 1 mg lysates were each incubated separately overnight at 4 °C with the following: rat anti-TMEM147-O immunoglobulin G (IgG) for input samples, rat anti-Hco-gal IgG for IP samples and normal rat IgG (Santa Cruz Biotechnology, Dallas, Texas, USA) for negative control samples in forward IP; rat anti-Hco-gal IgG for input samples, rat anti-TMEM147-O IgG for IP samples, normal rat IgG for negative control samples in reverse IP. Immune complexes were precipitated by 20 μl protein A/G PLUS-Agarose beads (Santa Cruz Biotechnology, Texas, USA) following the manufacturer’s protocol.
Immunoblotting assays were performed following co-IP according to the methods previously described . The membrane was incubated with the respective primary antibodies: rat anti-TMEM147-O IgG for forward IP experiment or rat anti-Hco-gal IgG for reverse IP experiment overnight at 4 °C. Antibodies and the production of antibodies used in these experiments are described in Additional file 1: Protocols (Production of antibody); Additional file 2: Table S1; and Additional file 3: Figures S1–S4.
Detection of the localization of TMEM147 in PBMC by immunofluorescence (IF)
The IF assay was performed according to previous research . The fixed and permeabilized PBMC (105 cells/sample) were incubated with 0.5 μg of the respective primary antibodies, rat anti-TMEM147-O IgG or negative rat IgG (for negative control) and incubated with the secondary antibody coupled to Cyanine dyes 3 (Cy3) fluorescent dye (Beyotime Biotechnology, Haimen, Jiangsu, China) (1:300). Cells were then stained with 5 μM 3,3'-Dioctadecyloxacarbocyanine (DiOC18(3); Beyotime Biotechnology, Haimen, Jiangsu, China) and 1.5 μM 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI; Sigma, St. Louis, Missouri, USA), respectively, for 6 min each. Protein localization was observed with a 100× oil objective lens on a laser scanning confocal microscope (LSM710, Zeiss, Jena, Thuringia, Germany). Digital images were captured using the Zeiss microscope software package ZEN 2012 (Zeiss, Jena, Thuringia, Germany).
Detection of the distribution of TMEM147 in T cells, B cells and monocytes in PBMC by flow cytometry
The distribution of TMEM147 in T cells and monocytes in PBMC were detected as previously described using flow cytometry . PBMC (106 cells/reaction), isolated as described above, were firstly stained with antibodies: 1 μg mouse anti-bovine cluster of differentiation 2-fluorescein isothiocyanate (CD2-FITC), 1 μg mouse anti-bovine cluster of differentiation 21-fluorescein isothiocyanate (CD21-FITC) and 1 μg mouse anti-bovine cluster of differentiation 14-fluorescein isothiocyanate (CD14-FITC) (AbDSerotec, Bio-Rad Laboratories, Hercules, California, USA), respectively, at 4 °C for 30 min. After permeabilization and washing, cells were then incubated with 1 μg rat anti-TMEM147-O IgG for 1 h and chicken anti-rat immunoglobulin G-phycoerythrin (IgG-PE) (1:300, Santa Cruz Biotechnology, Dallas, Texas, USA) for 30 min at room temperature. Normal mouse immunoglobulin G1-fluorescein isothiocyanate (IgG1-FITC) (Santa Cruz Biotechnology, Dallas, Texas, USA) and negative rat IgG (1 μg) were used to set a ‘fluorescence minus one’ control. Samples were examined on a FACS Calibur™ flow cytometer (BD Biosciences, San Jose, California, USA). Data were analyzed using FlowJo 7.6 software (Tree Star, Ashland, Oregon, USA).
Small interfering RNA and cell treatment
Three small interfering RNAs (siRNA) were designed to knockdown the tmem147 gene (Additional file 2: Table S2). TMEM147-siRNA-1, with the highest interference efficiency, was selected for further experiments (Additional file 1: Protocols (Transfection procedures for siRNA) and Additional file 3: Figure S5). The siRNAs used in this study were chemically synthesized by Invitrogen (Life Technologies, Shanghai, China) and dissolved in RNase-free water to 20 μM. The time required for knockdown was also determined (Additional file 1: Protocols (Transfection procedures for siRNA) and Additional file 3: Figure S5). The non-specific siRNA (ns siRNA) sequences used in the experiment are listed in Additional file 2: Table S2.
Cells were treated with siRNA transfection and then rHco-gal-m was added as stimulation. The concentration of rHco-gal-m (40 μg/ml) has been demonstrated to produce a distinct biological response without causing toxicity to the cells by previous dose response studies [26, 29, 30]. Cells for the RNAi period are shown as a blank group (group 1), ns siRNA group (group 2), ns siRNA/g group (group 3), 147-siRNA group (group 4) and 147-siRNA/g group (group 5), which were incubated with an equal volume of reduced serum medium (group 1), ns siRNA (groups 2 and 3) and TMEM147-siRNA-1 (groups 4 and 5), for 60 h at a concentration of 1 × 106 cells/ml. rHco-gal-m in all RNAi groups (groups 3 and 5) was added 12 h before the end of the RNAi period .
Cell proliferation assay
At the end of the RNAi period, cell proliferation assay of PBMC was performed as previously described using a cell counting kit-8 assay reagent (Beyotime Biotechnology, Haimen, Jiangsu, China) . Cells in the blank group served as controls and the OD450 was set as 100 %. The cell proliferation index was calculated using the formula: OD450 group/OD450 control.
Cell phagocytosis assay
Monocytes were collected at the end of the RNAi period and incubated with 1 mg/ml fluorescein isothiocyanate-dextran (FITC-dextran) (Sigma, St. Louis, Missouri, USA) in Roswell Park Memorial Institute 1640 (RPMI 1640; GIBCO, Grand Island, New York, USA) at 37 °C for 1 h . The reaction was stopped using cold phosphate buffered saline (PBS) containing 2 % fetal bovine serum. Cells were washed three times and resuspended in PBS containing 2 % paraformaldehyde. The FITC-dextran internalization of monocytes was analyzed by flow cytometry (BD Biosciences, San Jose, California, USA). Data were analyzed using FlowJo 7.6 software (Tree Star, Ashland, Oregon, USA) and the cell phagocytosis index was calculated by considering the statistical data of Median Fluorescence Intensity (MFI) values in the blank group as 100 %.
Measurement of nitric oxide (NO) production
According to a previous study using Total Nitric Oxide Assay Kit (Beyotime Biotechnology, Haimen, Jiangsu, China) , the NO production assay was performed by detecting intracellular nitrite in the PBMC with the Griess assay . Absorbance was determined using a plate reader (Bio-Rad Laboratories, Hercules, California, USA) at 540 nm (OD540) and then converted to micromoles per liter using a standard curve generated by the addition of 0 to 80 μmol/l of sodium nitrite to fresh culture media.
Cell migration assay
At the end of the RNAi period, the migration assay of PBMC was performed as previously described using Millicell® insert with 8.0 μm pores (Merck Millipore, Darmstadt, Hessen, Germany) . The cells that migrated through the membrane into the lower chamber were determined with a Neubauer counting chamber. The results were presented as percentages of the seeded PBMC. Each experiment was performed in triplicate.
The Annexin V-FITC kit (Miltenyi Biotec, Bergisch Gladbach, Nordrhein-Westfalen, Germany) was used to estimate cell apoptosis, according to the manufacturer’s instructions. At the end of RNAi period, cells (1 × 106 cells/ml) were harvested and used to check the apoptosis using the flow cytometer (BD Biosciences, San Jose, California, USA.). Data were analyzed using FlowJo 7.6 software (Tree Star, Ashland, Oregon, USA).
Detection of cytokine transcription
40 μl of vehicle (PBS, unstimulated negative control) or rHco-gal-m at 1 μg/μl was added to the PBMC to yield a final volume of 1 ml per well and incubated for 12 h before the end of the RNAi period. The detection of cytokine transcription was performed as previously described by real-time PCR . The reactions and conditions of real-time PCR are detailed in Additional file 1: Protocols (Real-time PCR reactions and conditions), and the primers for real-time PCR are listed in Additional file 2: Table S3. The amplification efficiencies of all targets and endogenous reference genes were verified to be similar by real-time PCR (Additional file 2: Table S3). Raw cycle thresholds (Ct), obtained from the ABI Prism 7500 software (version 2.0.6; Applied Biosystems, Foster City, California, USA), were used for the comparative Ct (2-ΔΔCt) method .
Statistical analysis for significant differences was performed using the Graphpad Premier 6.0 software package (Graphpad Prism, San Diego, California, USA) at P < 0.001. Data were expressed as the mean ± the standard deviation (SD).
TMEM147 is a novel binding protein for Hco-gal-m and -f
YTH screening assays were performed to find the binding partners of Hco-gal-m and -f from a goat PBMC cDNA library constructed previously . Following two rounds of YTH screening, 20 clones encoding proteins were found to interact with the Hco-gal-m and -f proteins in yeast cells. Using DNA sequencing and BLASTn of GenBank, four genes products were identified. Besides TMEM63A, one of the products was TMEM147 (NCBI accession number JQ923484). Moreover, the amino acid sequences of TMEM147 were used to predict the transmembrane structure by TMHMM Server v.2.0 (http://www.cbs.dtu.dk/services/TMHMM/), and the results detailed in Additional file 3: Figure S4 indicate that TMEM147 has 7 transmembrane domains and largely resides within the membrane. As the same candidate proteins were identified using either Hco-gal-m or Hco-gal-f as bait, only rHco-gal-m was used in subsequent experiments as a representative protein [27, 28].
Co-IP assays demonstrated that rHco-gal-m could bind to TMEM147
TMEM147 was localized to the cell membrane and within the cell membrane in PBMC
TMEM147 was expressed in T cells, B cells and monocytes of goat PBMC
rHco-gal-m affected PBMC proliferation by the interaction with TMEM147
rHco-gal-m affected monocytes phagocytosis by the interaction with TMEM147
rHco-gal-m affected PBMC nitric oxide production by the interaction with TMEM147
The interaction of rHco-gal-m with TMEM147 might not mediate PBMC migration
rHco-gal-m affected the apoptosis of goat PBMC by the interaction with TMEM147
The interaction of rHco-gal-m with TMEM147 affected the transcription of cytokines in goat PBMC
Transcription of IL-10 (ANOVA, F (3, 8) = 199.2, P < 0.0001) and TGF-β1 (ANOVA, F (3, 8) = 364.3, P < 0.0001) in rHco-gal-m-treated cells were significantly diminished by TMEM147 siRNA-treatment, but unaffected (ANOVA, F (3, 8) = 199.2, P = 0.1505 for IL-10; F (3, 8) = 364.3, P = 0.0080 for TGF-β1) in PBS-treated cells (Fig. 9b, c). Notably, the transcription of IFN-γ in TMEM147 siRNA group were all prominently decreased (ANOVA, F (3, 8) = 949.5, P < 0.0001) compared to the non-specific siRNA group by the same stimuli (Fig. 9a).
TMEM147 is a highly conserved membrane protein amongst mammals and widely expressed in many peripheral and central tissues whose function is still being elucidated. Rosemond et al.  reported that TMEM147 is a binding protein of M3R M3 muscarinic acetylcholine receptor and might act as a potent negative regulator of M3R function by changing the stimulatory effects of carbachol on H508 human colon cancer cell proliferation and p90 ribosomal S Kinase (p90RSK) activation. Dettmer et al.  suggested that TMEM147 was also a binding partner of a membrane protein complex, the Nicastrin-like protein-Nodal modulator (Nicalin-NOMO) complex. Nodal factors can initiate signaling cascades and drive the transcription of specific genes which affect cell proliferation . Combined, these indicate that TMEM147 may have important functional roles in physiological processes. In this study, YTH screening and co-IP assays demonstrated that TMEM147 could bind to Hco-gal-m and Hco-gal-f. After knockdown of the tmem147 gene by RNAi, the influence of rHco-gal-m on proliferation, phagocytosis, nitric oxide production, apoptosis and cytokine expression of the siRNA-treated cells were all changed. This indicated that TMEM147 mediated the regulation of rHco-gal-m on the goat PBMC.
Rosemond et al.  and Dettmer et al.  reported that TMEM147 localized to the endoplasmic reticulum (ER) membranes of co-transfected mammalian cells (COS-7 cells) and was enriched in ER membranes in HeLa cells. However, in this study, it was found that TMEM147 localized to both the cell surface and the inside of cells (Fig. 2). It was therefore suspected that TMEM147 might have different localizations in different kinds of cells. Our data suggest that TMEM147 was expressed by all B cells, monocytes and most T cells in goat PBMC (Fig. 3). Wang et al.  reported that different PBMC subsets had distinct responses to stimulation of rHco-gal-m/f. Hence, the specific functions of TMEM147 expressed by specific cell subsets and the nature and functions of these TMEM147-negative cells needs to be further investigated.
It has been suggested that TMEM147 was implicated in the regulation of cell proliferation [34, 35], and rHco-gal-m inhibited the proliferation of T cells in goat PBMC in vitro [27, 28]. In this study, it was found that the co-incubation with rHco-gal-m significantly suppressed the PBMC proliferation in the ns siRNA-treated cells, while the PBMC proliferation in rHco-gal-m-treated cells was significantly increased after TMEM147 siRNA-treatment (Fig. 4). It indicated that TMEM147 played a key role in the regulation of rHco-gal-m on the PBMC proliferation. In a previous study, TMEM63A specific siRNA-treatment could not effectively reverse the inhibition of rHco-gal-m on the PBMC proliferation . So the modulation of cell proliferation in goat PBMC by rHco-gal-m was mainly mediated by TMEM147, but not TMEM63A.
It was demonstrated that phagocytosis could make a contribution to the clearance of damaged cells and host defense against pathogens, and a number of galectin family members have been shown to play an important role in phagocytosis . In this study, the phagocytosis of monocytes in the ns siRNA/g group was significantly suppressed by rHco-gal-m (Fig. 5). After TMEM147 siRNA-treatment, the phagocytosis of monocytes in the 147 siRNA/g group was significantly increased compared to the 147 siRNA group and ns siRNA/g group (Fig. 5). It is therefore expected that the interaction between Hco-gal-m and TMEM147 plays an important role in the modulation of cell phagocytosis. In previous study , it was shown that the effective potency of TMEM63A was the same to TMEM147. However, the real functions of these two proteins or their collaboration in the regulation of phagocytosis still needs further research.
Nitric oxide (NO) has been implicated in host non-specific defence against a variety of infections and play roles in a number of parasitic infections such as malaria, toxoplasmosis, leishmaniosis, trypanosomosis and schistosomosis . In this study, nitric oxide production in 147 siRNA/g group was significantly increased compared to the 147 siRNA group and ns siRNA/g group (Fig. 6). In the study of TMEM63A, the nitric oxide production in the 63A siRNA/g group was significantly increased compared to the ns siRNA/g group, while the production in 63A siRNA/g group was still significantly lower than that in the 63A siRNA group . These findings revealed that rHco-gal-m inhibited the nitric oxide production by interactions with TMEM147 and TMEM63A. However, TMEM147 might play a greater role than TMEM63A in the rHco-gal-m-mediated regulation of nitric oxide production on goat PBMC.
It was demonstrated that rHco-gal-m/f significantly decreased the ability of cell migration in goat PBMC . This description was further supported by our data. However, the significant decrease induced by rHco-gal-m was not changed by the TMEM147 siRNA-treatment (Fig. 7). In the study of TMEM63A, the suppression of cell migration induced by rHco-gal-m in the ns siRNA/g group was significantly changed by TMEM63A siRNA-treatment . These results indicated that it was TMEM63A, not TMEM147, involved in the rHco-gal-m-mediated regulation of cell migration on goat PBMC.
It was reported that many members of galectin family were implicated in the regulation of cell apoptosis [39–41], and rHco-gal-m could induce cell apoptosis in goat PBMC [26, 28]. In this study, TMEM147 siRNA-treatment could significantly decrease cell apoptosis induced by rHco-gal-m (Fig. 8). Our data strongly suggest that TMEM147 was involved in the regulation of PBMC apoptosis induced by rHco-gal-m.
Cytokines play an important role in regulating development, differentiation and expression of functional effecters of the immune system, and the profile of immune reactions depends on cytokine contents at the recognition of parasite [42–45]. Recent studies reported that galectin-1 could affect the Th1/Th2 balance by mediating Th1 cell apoptosis and stimulating Th2 cytokine secretion , and IL-10 played important roles in mediating the immunosuppressive activities of galectin-1 . Our previous studies demonstrated that rHco-gal-m increased the mRNA transcript levels of IL-10 and TGF-β1, whilst decreasing the transcription levels of IFN-γ [27, 29]. In this study, TMEM147 siRNA-treatment significantly decreased the transcript levels of IFN-γ either in the rHco-gal-m treated group or in the PBS control group (Fig. 9). In the study of TMEM63A, TMEM63A siRNA-treatment could significantly enhance the transcription of IFN-γ in both rHco-gal-m treated and PBS control groups . It was suggested that the inhibition of IFN-γ transcription induced by rHco-gal-m was mainly mediated by TMEM63A. The results also showed that the enhancement of IL-10 and TGF-β1 transcription induced by rHco-gal-m in the ns siRNA groups were reversed by TMEM147 siRNA-treatment (Fig. 9), while slightly decreased by TMEM63A siRNA-treatment . These indicated that the rHco-gal-m enhanced the transcription of IL-10 and TGF-β1 mainly through the interaction with TMEM147. The real regulation mechanisms of cytokines requires further research.
Our data strongly suggest that TMEM147 is a binding partner or a membrane receptor for Hco-gal-m/f in goat PBMC. The interaction of galectin with TMEM147 mainly mediated cell proliferation, cell apoptosis, transcription of IL-10 and TGF-β1 of goat PBMC. This membrane protein, together with TMEM63A, was also related to the regulation of galectin on phagocytosis and nitric oxide production of goat PBMC. It is worth noting that TMEM63A might play a greater role than TMEM147 in the regulation of galectin in the migration and IFN-γ transcription of goat PBMC. These findings delivered new clues to the elucidation of the mechanisms involved in immune evasion by nematodes and in parasite-host interactions. However, the detailed functions of TMEM147 and TMEM63A in the regulation of galectin on the goat PBMC are still needed for further research.
CD14-FITC, cluster of differentiation 14-fluorescein isothiocyanate; CD21-FITC, cluster of differentiation 21-fluorescein isothiocyanate; CD2-FITC, cluster of differentiation 2-fluorescein isothiocyanate; co-IP, co-immunoprecipitation; Cy3, Cyanine dyes 3; DAPI, 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; DiOC18(3), 3,3'′-Dioctadecyloxacarbocyanine; Hco-gal-m/f, galectins of male and female Haemonchus contortus; Her-2, epidermal growth factor receptor-2; IFN-γ, interferon-γ; IgG, immunoglobulin G; IgG-PE, immunoglobulin G-phycoerythrin; IL-10, interleukin-10; IL-1β, interleukin-1β; IL-4, interleukin-4; L3s, third-stage larvae; LPG, Lipophosphoglycan; MFI, Median Fluorescence Intensity; MUC1, mucin 1; NFkB, nuclear factor-kappa B; Nicalin-NOMO, Nicastrin-like protein-Nodal modulator; Ns, non-specific; p90RSK, p90 ribosomal S Kinase; PBMC, peripheral blood mononuclear cells; PBS, phosphate buffered saline; rHco-gal-m, recombinant galectins of male Haemonchus contortus; rHco-gal-m/f, recombinant galectins of male and female Haemonchus contortus; RNAi, RNA interference; siRNA, small interfering RNA; TGF-β1, transforming growth factor-β1; Th1, helper T cell 1; Th2, helper T cell 2; TMEM147, transmembrane protein 147; TMEM63A, transmembrane protein 63A; TNF-α, tumor necrosis factor-α; YTH, yeast two-hybrid.
We gratefully thank Muhammad Ehsan and ZhenChao Zhang for valuable suggestions.
This work was funded by grants from the National Natural Science Foundation of P.R. China (Grant No.31172308), the National Key Basic Research Program (973 Program) of P.R. China (Grant No.2015CB150300) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
Availability of data and material
LXR directed the project and participated in the coordination and management of the study. LY and YC performed the laboratory tests and the data analysis and wrote the manuscript. WLK and LMM conducted flow cytometry and provided input into the experimental design. WYJ and WYL obtained blood samples and isolated the cells. YRF, SXK and XLX provided new analytical reagents and tools. All authors read and approved the final version of the manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
The treatments of animals in our research were in conformity with the guidelines of the Animal Ethics Committee, Nanjing Agricultural University, China. All animal experiments abided by the guidelines of the Animal Welfare Council of China. The protocols of our experiments were all approved by the Science and Technology Agency of Jiangsu Province. The approval ID is SYXK (SU) 2010–0005.
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- Kaminsky R, Ducray P, Jung M, Clover R, Rufener L, Bouvier J, et al. A new class of anthelmintics effective against drug-resistant nematodes. Nature. 2008;452(7184):176–80.View ArticlePubMedGoogle Scholar
- Nikolaou S, Gasser RB. Prospects for exploring molecular developmental processes in Haemonchus contortus. Int J Parasitol. 2006;36(8):859–68.View ArticlePubMedGoogle Scholar
- Troncoso MF, Elola MT, Croci DO, Rabinovich GA. Integrating structure and function of 'tandem-repeat' galectins. Front Biosci (Schol Ed). 2012;4:864–87.Google Scholar
- Arthur CM, Baruffi MD, Cummings RD, Stowell SR. Evolving mechanistic insights into galectin functions. Methods Mol Biol. 2015;1207:1–35.View ArticlePubMedGoogle Scholar
- Liu FT, Yang RY, Hsu DK. Galectins in acute and chronic inflammation. Ann N Y Acad Sci. 2012;1253:80–91.View ArticlePubMedGoogle Scholar
- Rabinovich GA, Rubinstein N. [Galectins: a novel family of proteins involved in the regulation of the immune response. Implications in immunopathological processes]. Medicina (B Aires). 2001;61(1):85–92.Google Scholar
- Vasta GR. Roles of galectins in infection. Nat Rev Microbiol. 2009;7(6):424–38.View ArticlePubMedPubMed CentralGoogle Scholar
- Rabinovich GA, Toscano MA, Ilarregui JM, Rubinstein N. Shedding light on the immunomodulatory properties of galectins: novel regulators of innate and adaptive immune responses. Glycoconj J. 2004;19(7-9):565–73.View ArticlePubMedGoogle Scholar
- Young AR, Meeusen EN. Galectins in parasite infection and allergic inflammation. Glycoconj J. 2004;19(7-9):601–6.View ArticlePubMedGoogle Scholar
- Okumura CY, Baum LG, Johnson PJ. Galectin-1 on cervical epithelial cells is a receptor for the sexually transmitted human parasite Trichomonas vaginalis. Cell Microbiol. 2008;10(10):2078–90.View ArticlePubMedPubMed CentralGoogle Scholar
- Zuniga E, Gruppi A, Hirabayashi J, Kasai KI, Rabinovich GA. Regulated expression and effect of galectin-1 on Trypanosoma cruzi-infected macrophages: modulation of microbicidal activity and survival. Infect Immun. 2001;69(11):6804–12.View ArticlePubMedPubMed CentralGoogle Scholar
- Toscano MA, Tongren JE, de Souza JB, Liu FT, Riley EM, Rabinovich GA. Endogenous galectin-3 controls experimental malaria in a species-specific manner. Parasite Immunol. 2012;34(7):383–7.View ArticlePubMedGoogle Scholar
- Oakley MS, Majam V, Mahajan B, Gerald N, Anantharaman V, Ward JM, et al. Pathogenic roles of CD14, galectin-3, and OX40 during experimental cerebral malaria in mice. PLoS One. 2009;4(8):e6793.View ArticlePubMedPubMed CentralGoogle Scholar
- Bernardes ES, Silva NM, Ruas LP, Mineo JR, Loyola AM, Hsu DK, et al. Toxoplasma gondii infection reveals a novel regulatory role for galectin-3 in the interface of innate and adaptive immunity. Am J Pathol. 2006;168(6):1910–20.View ArticlePubMedPubMed CentralGoogle Scholar
- Pineda MA, Cuervo H, Fresno M, Soto M, Bonay P. Lack of Galectin-3 prevents cardiac fibrosis and effective immune responses in a murine model of Trypanosoma cruzi infection. J Infect Dis. 2015;212(7):1160–71.View ArticlePubMedGoogle Scholar
- Sato S, Bhaumik P, St-Pierre G, Pelletier I. Role of galectin-3 in the initial control of Leishmania infection. Crit Rev Immunol. 2014;34(2):147–75.View ArticlePubMedGoogle Scholar
- Pelletier I, Hashidate T, Urashima T, Nishi N, Nakamura T, Futai M, et al. Specific recognition of Leishmania major poly-beta-galactosyl epitopes by galectin-9: possible implication of galectin-9 in interaction between L. major and host cells. J Biol Chem. 2003;278(25):22223–30.View ArticlePubMedGoogle Scholar
- Katoh S, Oomizu S, Niki T, Shimizu H, Obase Y, Korenaga M, et al. Possible regulatory role of galectin-9 on Ascaris suum-induced eosinophilic lung inflammation in mice. Int Arch Allergy Immunol. 2012;158 Suppl 1:58–65.View ArticlePubMedGoogle Scholar
- Hoorens P, Rinaldi M, Mihi B, Dreesen L, Grit G, Meeusen E, et al. Galectin-11 induction in the gastrointestinal tract of cattle following nematode and protozoan infections. Parasite Immunol. 2011;33(12):669–78.View ArticlePubMedGoogle Scholar
- Athanasiadou S, Pemberton A, Jackson F, Inglis N, Miller HR, Thevenod F, et al. Proteomic approach to identify candidate effector molecules during the in vitro immune exclusion of infective Teladorsagia circumcincta in the abomasum of sheep. Vet Res. 2008;39(6):58.View ArticlePubMedGoogle Scholar
- Robinson N, Pleasance J, Piedrafita D, Meeusen EN. The kinetics of local cytokine and galectin expression after challenge infection with the gastrointestinal nematode, Haemonchus contortus. Int J Parasitol. 2011;41(5):487–93.View ArticlePubMedGoogle Scholar
- Mori Y, Akita K, Yashiro M, Sawada T, Hirakawa K, Murata T, et al. Binding of Galectin-3, a beta-Galactoside-binding lectin, to MUC1 protein enhances phosphorylation of extracellular signal-regulated Kinase 1/2 (ERK1/2) and Akt, promoting tumor cell malignancy. J Biol Chem. 2015;290(43):26125–40.View ArticlePubMedGoogle Scholar
- Shetty P, Bargale A, Patil BR, Mohan R, Dinesh US, Vishwanatha JK, et al. Cell surface interaction of annexin A2 and galectin-3 modulates epidermal growth factor receptor signaling in Her-2 negative breast cancer cells. Mol Cell Biochem. 2016;411(1-2):221–33.View ArticlePubMedGoogle Scholar
- Chun-Hua LI. Cloning, Characterization and sequence analysis of galectin cDNA of female and male adult worms of Haemonchus contortus. J Agric Biotechnol. 2005;13:86–91.Google Scholar
- Wang W, Yuan C, Wang S, Song X, Xu L, Yan R, et al. Transcriptional and proteomic analysis reveal recombinant galectins of Haemonchus contortus down-regulated functions of goat PBMC and modulation of several signaling cascades in vitro. J Proteomics. 2014;98:123–37.View ArticlePubMedGoogle Scholar
- Sun YM, Yan RF, Muleke CI, Zhao GW, Xu LX, Li XR. Recombinant galectins of Haemonchus contortus parasite induces apoptosis in the peripheral blood lymphocytes of goat. Int J Pept Res Ther. 2007;13(3):387–92.View ArticleGoogle Scholar
- Yuan C, Zhang H, Wang W, Li Y, Yan RF, Xu LX, et al. Transmembrane protein 63A is a partner protein of Haemonchus contortus galectin in the regulation of goat peripheral blood mononuclear cells. Parasit Vectors. 2015;8:211. doi:10.1186/s13071-015-0816-3.
- Wang W, Wang S, Zhang H, Yuan C, Yan R, Song X, et al. Galectin Hco-gal-m from Haemonchus contortus modulates goat monocytes and T cell function in different patterns. Parasit Vectors. 2014;7:342.View ArticlePubMedPubMed CentralGoogle Scholar
- Sun YM, Yan RF, Li-Xin XU, Muleke CI, Xiang-Rui LI. Recombinant galectins of Haemonchus contortus inhibit goat cytokine mRNA transcription of peripheral blood mononuclear cells. Agric Sci China. 2007;6(10):1262–8.View ArticleGoogle Scholar
- Rathore DK, Suchitra S, Saini M, Singh BP, Joshi P. Identification of a 66 kDa Haemonchus contortus excretory/secretory antigen that inhibits host monocytes. Vet parasitol. 2006;138(3-4):291–300.View ArticlePubMedGoogle Scholar
- Chen WX, Zhang WY, Shen WB, Wang KC. Effects of the acid polysaccharide fraction isolated from a cultivated Cordyceps sinensis on macrophages in vitro. Cell Immunol. 2010;262(1):69–74.View ArticlePubMedGoogle Scholar
- Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15 N]nitrate in biological fluids. Anal Biochem. 1982;126(1):131–8.View ArticlePubMedGoogle Scholar
- Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. Methods. 2001;25(4):402–8.View ArticlePubMedGoogle Scholar
- Rosemond E, Rossi M, McMillin SM, Scarselli M, Donaldson JG, Wess J. Regulation of M(3) muscarinic receptor expression and function by transmembrane protein 147. Mol Phys. 2011;79(2):251–61.Google Scholar
- Dettmer U, Kuhn PH, Abou-Ajram C, Lichtenthaler SF, Kruger M, Kremmer E, et al. Transmembrane protein 147 (TMEM147) is a novel component of the Nicalin-NOMO protein complex. J Biol Chem. 2010;285(34):26174–81.View ArticlePubMedPubMed CentralGoogle Scholar
- Massague J. TGF-beta signal transduction. Annu Rev Biochem. 1998;67:753–91.View ArticlePubMedGoogle Scholar
- Chen HY, Weng IC, Li CS, Wan L, Liu FT. Examination of galectins in phagocytosis. Methods Mol Biol. 2015;1207:201–13.View ArticlePubMedPubMed CentralGoogle Scholar
- Elzbieta WN. The role of nitric oxide (NO) in parasitic infections. Wiad Parazytol. 2004;50(4):665–78.Google Scholar
- Sturm A, Lensch M, Andre S, Kaltner H, Wiedenmann B, Rosewicz S, et al. Human galectin-2: novel inducer of T cell apoptosis with distinct profile of caspase activation. J immunol. 2004;173(6):3825–37.View ArticlePubMedGoogle Scholar
- Hsu DK, Yang RY, Saegusa J, Liu FT. Analysis of the intracellular role of galectins in cell growth and apoptosis. Methods Mol Biol. 2015;1207:451–63.View ArticlePubMedPubMed CentralGoogle Scholar
- Kang CW, Dutta A, Chang LY, Mahalingam J, Lin YC, Chiang JM, et al. Apoptosis of tumor infiltrating effector TIM-3 + CD8+ T cells in colon cancer. Sci Rep. 2015;5:15659.View ArticlePubMedPubMed CentralGoogle Scholar
- Kikodze N, Pantsulaia I, Rekhviashvili K, Iobadze M, Dzhakhutashvili N, Pantsulaia N, et al. Cytokines and T regulatory cells in the pathogenesis of type 1 diabetes. Georgian Med News. 2013;222:29–35.PubMedGoogle Scholar
- Gigante M, Ranieri E. TGF-beta promotes immune suppression by inhibiting Treg cell apoptosis. Immunotherapy. 2010;2(5):608.View ArticlePubMedGoogle Scholar
- Huang H, Li Y, Qi X. Cytokine signaling in the differentiation of innate effector cells. Jak-Stat. 2013;2(1):e23531.View ArticlePubMedPubMed CentralGoogle Scholar
- Doligalska M. Regulation of defence and allergic reaction in infections with parasites. Wiad Parazytol. 2000;46(1):3–20.PubMedGoogle Scholar
- Tang D, Gao J, Wang S, Yuan Z, Ye N, Chong Y, et al. Apoptosis and anergy of T cell induced by pancreatic stellate cells-derived galectin-1 in pancreatic cancer. Tumour Biol. 2015;36(7):5617–26.View ArticlePubMedGoogle Scholar
- Blois SM, Ilarregui JM, Tometten M, Garcia M, Orsal AS, Cordo-Russo R, et al. A pivotal role for galectin-1 in fetomaternal tolerance. Nat Med. 2007;13(12):1450–7.View ArticlePubMedGoogle Scholar