- Letter to the Editor
- Open Access
Molecular evidence of Rickettsia raoultii, “Candidatus Rickettsia barbariae” and a novel Babesia genotype in marbled polecats (Vormela peregusna) at the China-Kazakhstan border
Parasites & Vectorsvolume 11, Article number: 450 (2018)
In this study, two road-killed marbled polecats (Vormela peregusna) were molecularly analysed for tick-borne pathogens. Rickettsia raoultii, “Candidatus Rickettsia barbariae” and a novel Babesia genotype have been identified, for the first time in marbled polecat. DNA of this Babesia sp. genotype was also present in four out of 15 Haemaphysalis erinacei ticks collected from the Babesia PCR-positive marbled polecat. Results of this study suggest that marbled polecats may serve as reservoirs for these bacteria and protozoans.
Letter to the Editor
The marbled polecat (Vormela peregusna) is a small carnivorous mammal (Carnivora: Mustelidae) with a broad geographical range, extending from southeast Europe, through southwest and central Asia, to Mongolia and northern China. This species is the only member of the genus and has been listed as globally vulnerable, due to substantially declining populations . Apart from the loss of steppe habitats and desertification, infection with various pathogens may contribute to its decreasing numbers. At the same time, data are very limited on the epidemiological role of the marbled polecat as a reservoir of pathogens with veterinary or medical significance [2, 3]. Therefore, molecular investigations for pathogens in this endangered species have multifold importance.
In May and June 2014, two road-killed, female marbled polecats, were found around wetlands of Ebinur Lake (189 m above sea level; coordinates: 82°48'51"E, 45°04'22"N) in northwestern China, in the border region near Kazakhstan. Previously, 21 Haemaphysalis erinacei ticks collected from the two animals (15 from polecat #1 and six from polecat #2) were molecularly characterised at 16S mitochondrial gene region. Two of 15 ticks (13.33%) collected from marbled polecat #1 were infected with Rickettsia raoultii . The two animals were brought to the Xinjiang Uygur Autonomous Region Wildlife Management Office and then sent to the Laboratory of High Incidence of Local and Ethnic Diseases in Xinjiang for necropsy analyses. DNA extractions from the liver and spleen were carried out using the TIANamp Genomic DNA Kit (TIANGEN, Beijing, China). The presence of DNA from tick-borne pathogens was investigated by PCR amplification and sequencing of parts of the following genes: the 17-kDa surface antigen gene (17 kDa gene) of Rickettsia spp., the 5S-23S rRNA gene of Borrelia spp., the 16S rRNA gene of Anaplasmataceae, and the 18S rRNA gene of Babesia spp., as described previously [5,6,7]. All samples were negative for Borrelia spp. and Anaplasmataceae. To confirm the results and to compare additional genetic markers, further PCR and sequencing were performed, to detect the outer membrane protein A (ompA) and cell surface antigen 1 (sca1) genes of Rickettsia spp.  and the cytochrome b (cytb) gene of Babesia spp. . All PCRs were performed including double distilled water (ddH2O) as a negative control. Sequences were compared with GenBank data using the nucleotide BLAST program (http://www.ncbi.nlm.nih.gov/BLAST/). All obtained sequences were deposited in the GenBank database [17-kDa: MG674917 (R. raoultii) and MG674918 (“Candidatus Rickettsia barbariae”); ompA: MG662380 (R. raoultii) and MG662381 (“Candidatus Rickettsia barbariae”); sca1: MG662382 (R. raoultii) and MG662383 (“Candidatus Rickettsia barbariae”); 18S rRNA: MG799848 and MG813565; cytb: MG832590 and MG832591 (Babesia sp. from marbled polecat and H. erinacei, respectively)].
However, the liver and spleen samples of both marbled polecats were PCR-positive for rickettsiae. In animal #1, the ompA and sca1 sequences showed 100% identities with R. raoultii strain KhabarovskT (GenBank: CP010969). This result is in line with previous findings, i.e. two out of the fifteen ticks (i.e. 13.33%) collected from animal #1 harboured R. raoultii. The phylogenetic analysis showed that the genetic pattern was the same between R. raoultii from a marbled polecat #1 and R. raoultii previously found in H. erinacei ticks collected from marbled polecat #1 (Fig. 1) . In animal #2, “Candidatus Rickettsia barbariae” was identified, with 99.8% (552/553 bp) and 100% (443/443 bp) similarities to sequences available in GenBank [from Vermipsylla alakurt in China: KT284718 (sca1) and KU645284 (ompA)]. Results of sequence alignments were confirmed by phylogenetic analyses, placing rickettsiae detected here into the cluster of either R. raoultii or “Candidatus Rickettsia barbariae” (Fig. 1 and Additional file 1: Table S1).
Both Rickettsia spp. were detected for the first time in marbled polecats. Spotted fever group rickettsiae (Proteobacteria: Rickettsiales) are obligatory intracellular, Gram-negative bacteria, which can cause zoonotic disease after transmission by a blood-sucking arthropod . Rickettsia raoultii is one of the causative agents of human rickettsioses (SENLAT/TIBOLA/DEBONEL ). Until recently, R. raoultii has not been reported from mammals other than humans , but during the past years, this species was detected in Mongolian gazelle  and dogs . The most important tick vectors of R. raoultii appear to be Dermacentor spp., with transstadial and transovarial maintenance of this agent . Accordingly, a previous study revealed R. raoultii as the predominant species of Rickettsia found in D. nuttalli from Mongolian regions and in D. silvarum ticks in the border region between China and Russia [15, 16]. Therefore, simultaneous detection of R. raoultii in a marbled polecat (as shown here) and in H. erinacei removed from the same animal justify further studies on the vector role of this tick species, which is outside the typical vector range of R. raoultii.
“Candidatus Rickettsia barbariae” has been detected and described from ticks of domestic and wild animals [17,18,19,20] and humans  in the Mediterranean region. Our previous work also showed the presence of DNA of “Candidatus Rickettsia barbariae” in the tick Rhipicephalus turanicus  and the flea Vermipsylla alakurt  collected from sheep around the Taklamakan Desert in Xinjiang, northwestern China. To the best of our knowledge, “Candidatus Rickettsia barbariae” was not detected in any vertebrate hosts of the above ectoparasites, further increasing the significance of the present findings.
In addition to the above results, Babesia sp. DNA was detected both in four H. erinacei ticks and the organs of their parasitised host (marbled polecat #1). The 18S rRNA gene sequences were 100% identical between the marbled polecat and its PCR-positive ticks. However, this Babesia genotype had only 97.12% (1517/1562 bp) 18S rRNA gene similarity to the closest genotype in GenBank, detected in the blood of a racoon in Japan (GenBank: AB935168). The cytb gene sequence comparisons supported the uniqueness of this Babesia sp. from marbled polecat because the gene sequence showed 85.74% (433/505 bp) similarity to that of Babesia gibsoni (no corresponding cytb gene sequence was found for Babesia sp. from a racoon in GenBank). The phylogenetic analysis confirmed these results: this novel Babesia genotype clustered separately from the above racoon-associated piroplasm (GenBank: AB935168) and belonged to a phylogenetic group including Babesia spp. from Caniformia or tick species (i.e. H. concinna and from the present study H. erinacei) frequently infesting Caniformia (Fig. 2). The separation of this phylogenetic group from a cat-associated piroplasm (B. hongkongensis) was strongly (85%) supported (Fig. 2).
To the best of our knowledge, this is the first report on the presence of Babesia DNA in marbled polecat and H. erinacei ticks. Babesia spp. (Apicomplexa: Piroplasmida) are intraerythrocytic parasites, which have been reported from birds and mammals (including wild carnivores) worldwide [7, 23] and have ixodid ticks as their principal vectors . The 18S rRNA gene is the most widely used genetic marker for the identification of babesiae . Based on 18S rRNA gene sequence data and the topology of the phylogenetic tree (Fig. 2), the Babesia sp. detected here in the marbled polecat, and the attendant ticks was most closely related to a Babesia sp. from a racoon (GenBank: AB935168: reported from Osaka, Japan) and differed from all other piroplasmid species. Taking into account that the 18S rRNA gene may have very few nucleotide substitutions between closely related species [e.g. only 0.2% difference delineating B. divergens (GenBank: FJ944825) and B. capreoli (GenBank: AY726009) ], this new Babesia genotype most likely represents a species different from the one detected in raccoon in Japan. Unfortunately, morphological characterisation of this novel Babesia genotype was not possible because samples were obtained from road-killed (i.e. not freshly dead) animals, and Babesia are known to undergo quick degradation and morphological changes post-mortem .
In conclusion, results of this study suggest that marbled polecats may serve as reservoirs for R. raoultii, “Candidatus Rickettsia barbariae” and a novel Babesia genotype. Further studies are needed to evaluate if rickettsemia in this host species is of sufficient magnitude and duration to infect ticks, which is a known prerequisite for effective transmission of other tick-borne rickettsiae .
Dermacentor-borne necrosis, eschar and lymphadenopathy
human granulocytic anaplasmosis
International Union for Conservation of Nature
outer membrane protein A
surface cell antigen 1
International Union for Conservation of Nature (IUCN). IUCN Red List of Threatened Species, 2008. http://www.iucnredlist.org/. Accessed 25 Apr 2018.
Faizulin FG. Cutaneous leishmaniasis in a mottled polecat (Vormela peregusna Cueld). Med Parazitol. 1967;36:494–5.
Gherman CM, Sándor AD, Kalmár Z, Marinov M, Mihalca AD. First report of Borrelia burgdorferi sensu lato in two threatened carnivores: the marbled polecat, Vormela peregusna and the European mink, Mustela lutreola (Mammalia: Mustelidae). BMC Vet Res. 2012;8:137.
Guo LP, Mu LM, Xu J, Jiang SH, Wang AD, Chen CF, et al. Rickettsia raoultii in Haemaphysalis erinacei from marbled polecats, China-Kazakhstan border. Parasit Vectors. 2015;8:461.
Zhao SS, Li HY, Yin XP, Liu ZQ, Chen CF, Wang YZ. First detection of Candidatus Rickettsia barbariae in the flea Vermipsylla alakurt from north-western China. Parasit Vectors. 2016;9:325.
Wielinga PR, Gaasenbeek C, Fonville M, Boer DA, Vries DA, Dimmers W, et al. Longitudinal analysis of tick densities and Borrelia, Anaplasma, and Ehrlichia infections of Ixodes ricinus ticks in different habitat areas in The Netherlands. Appl Environ Microbiol. 2006;72:7594–601.
Bartley PM, Wilson C, Innes EA, Katzer F. Detection of Babesia DNA in blood and spleen samples from Eurasian badgers (Meles meles) in Scotland. Parasitology. 2017;144:1203–10.
Tian Z, Luo J, Zheng J, Xie J, Shen H, Yin H, et al. Phylogenetic analysis of Babesia species in China based on cytochrome b (COB) gene. Infect Genet Evol. 2013;13:36–40.
Parola P, Paddock CD, Socolovschi C, Labruna MB, Mediannikov O, Kernif T, et al. Update on tick-borne rickettsioses around the world: a geographic approach. Clin Microbiol Rev. 2013;26:657–702.
Parola P, Rovery C, Rolain JM, Brouqui P, Davoust B, Raoult D. Rickettsia slovaca and R. raoultii in tick-borne rickettsioses. Emerg Infect Dis. 2009;15:1105–8.
Karbowiak G, Biernat B, Stańczak J, Szewczyk T, Werszko J. The role of particular tick developmental stages in the circulation of tick-borne pathogens affecting humans in central Europe. 3. Rickettsiae. Ann Parasitol. 2016;62:89–100.
Li Y, Chen Z, Liu Z, Liu J, Yang J, Li Q, et al. First report of Theileria and Anaplasma in the Mongolian gazelle, Procapra gutturosa. Parasit Vectors. 2014;7:614.
Liesner JM, Krücken J, Schaper R, Pachnicke S, Kohn B, Müller E, et al. Vector-borne pathogens in dogs and red foxes from the federal state of Brandenburg, Germany. Vet Parasitol. 2016;224:44–51.
Mediannikov O, Matsumoto K, Samoylenko I, Drancourt M, Roux V, Rydkina E, et al. Rickettsia raoultii sp. nov., a spotted fever group rickettsia associated with Dermacentor ticks in Europe and Russia. Int J Syst Evol Microbiol. 2008;58:1635–9.
Speck S, Derschum H, Damdindorj T, Dashdavaa O, Jiang J, Kaysser P, et al. Rickettsia raoultii, the predominant rickettsia found in Mongolian Dermacentor nuttalli. Ticks Tick Borne Dis. 2012;3:227–31.
Wen J, Jiao D, Wang JH, Yao DH, Liu ZX, Zhao G, et al. Rickettsia raoultii, the predominant rickettsia found in Dermacentor silvarum ticks in China-Russia border areas. Exp Appl Acarol. 2014;63:579–85.
Sousa RD, Barata C, Vitorino L, Santos-Silva M, Carrapato C, Torgal J, et al. Rickettsia sibirica isolation from a patient and detection in ticks, Portugal. Emerg Infect Dis. 2006;12:1103–8.
Mura A, Masala G, Tola S, Satta G, Fois F, Piras P, et al. First direct detection of rickettsial pathogens and a new rickettsia, “Candidatus Rickettsia barbariae”, in ticks from Sardinia, Italy. Clin Microbiol Infect. 2008;14:1028–33.
Chochlakis D, Ioannou I, Sandalakis V, Dimitriou T, Kassinis N, Papadopoulos B, et al. Spotted fever group rickettsiae in ticks in Cyprus. Microb Ecol. 2012;63:314–23.
Pereira A, Parreira R, Cotão AJ, Nunes M, Vieira ML, Azevedo F, et al. Tick-borne bacteria and protozoa detected in ticks collected from domestic animals and wildlife in central and southern Portugal. Ticks Tick Borne Dis. 2018;9:225–34.
Papa A, Xanthopoulou K, Kotriotsiou T, Papaioakim M, Sotiraki S, Chaligiannis I, et al. Rickettsia species in human-parasitising ticks in Greece. Trans R Soc Trop Med Hyg. 2016;110:299–304.
Guo LP, Jiang SH, Liu D, Wang SW, Chen CF, Wang YZ. Emerging spotted fever group rickettsiae in ticks, northwestern China. Ticks Tick Borne Dis. 2016;7:1146–50.
Kawabuchi T, Tsuji M, Sado A, Matoba Y, Asakawa M, Ishihara C. Babesia microti-like parasites detected in feral racoons (Procyon lotor) captured in Hokkaido, Japan. J Vet Med Sci. 2005;67:825–7.
Hunfeld KP, Hildebrandt A, Gray JS. Babesiosis: recent insights into an ancient disease. Int J Parasitol. 2008;38:1219–37.
Malandrin L, Jouglin M, Sun Y, Brisseau N, Chauvin A. Redescription of Babesia capreoli (Enigk and Friedhoff, 1962) from roe deer (Capreolus capreolus): isolation, cultivation, host specificity, molecular characterisation and differentiation from Babesia divergens. Int J Parasitol. 2010;40:277–84.
Demeter Z, Palade EA, Balogh E, Jakab C, Farkas R, Tánczos B, et al. Postmortem small Babesia-like morphology of Babesia canis. Acta Vet Hung. 2011;59:427–32.
Telford SR, Parola P. Arthropods and Rickettsiae. In: Parola P, Raoult D, editors. Rickettsial diseases, Infectious diseases and therapy collection. New York: Informa Healthcare; 2007.
The authors thank the contributions by the staff at the School of Medicine, Shihezi University.
This research was supported in part by the National Key Research & Development Program of China (2018ZX10101002, 2017YFD0500304 and 2016YFC1202002), National Natural Science Foundation of China (81560338 and U1503283), State Key Laboratory of Veterinary Etiological Biology (SKLVEB2017KEKT009), and International Scientific and Technological Cooperation in Bingtuan (2016AH001).
Availability of data and materials
The sequences obtained and analysed during the present study are deposited in the GenBank database, under the accession numbers MG674917, MG662380 and MG662382 (R. raoultii); MG674918, MG662381 and MG662383 (“Candidatus Rickettsia barbariae”); MG799848, MG813565, MG832590 and MG832591 (Babesia sp.). All other relevant data are included in the article.
This study was approved by the Animal Ethics Committee of Shihezi University (Approval No. AECSU2014-03).
Consent for publication
The authors declare that they have no competing interests.
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About this article
- “Candidatus Rickettsia barbariae”
- China-Kazakhstan border
- Haemaphysalis erinacei
- Marbled polecat
- Rickettsia raoultii