- Open Access
Time-of-day of blood-feeding: effects on mosquito life history and malaria transmission
Parasites & Vectors volume 12, Article number: 301 (2019)
Biological rhythms allow organisms to compartmentalise and coordinate behaviours, physiologies, and cellular processes with the predictable daily rhythms of their environment. There is increasing recognition that the biological rhythms of mosquitoes that vector parasites are important for global health. For example, whether perturbations in blood foraging rhythms as a consequence of vector control measures can undermine disease control. To address this, we explore the impacts of altered timing of blood-feeding on mosquito life history traits and malaria transmission.
We present three experiments in which Anopheles stephensi mosquitoes were fed in the morning or evening on blood that had different qualities, including: (i) chemical-induced or (ii) Plasmodium chabaudi infection-induced anaemia; (iii) Plasmodium berghei infection but no anaemia; or (iv) stemming from hosts at different times of day. We then compared whether time-of-day variation in blood meal characteristics influences mosquito fitness proxies relating to survival and reproduction, and malaria transmission proxies.
Mosquito lifespan is not influenced by the time-of-day they received a blood meal, but several reproductive metrics are affected, depending on other blood characteristics. Overall, our data suggest that receiving a blood meal in the morning makes mosquitoes more likely to lay eggs, lay slightly sooner and have a larger clutch size. In keeping with previous work, P. berghei infection reduces mosquito lifespan and the likelihood of laying eggs, but time-of-day of blood-feeding does not impact upon these metrics nor on transmission of this parasite.
The time-of-day of blood-feeding does not appear to have major consequences for mosquito fitness or transmission of asynchronous malaria species. If our results from a laboratory colony of mosquitoes living in benign conditions hold for wild mosquitoes, it suggests that mosquitoes have sufficient flexibility in their physiology to cope with changes in biting time induced by evading insecticide-treated bed nets. Future work should consider the impact of multiple feeding cycles and the abiotic stresses imposed by the need to forage for blood during times of day when hosts are not protected by bed nets.
Daily rhythms are a ubiquitous feature of life . For example, circadian clocks are thought to enable organisms to coordinate with environmental periodicity in factors such as light/dark, humidity, UV exposure . Interactions with predators, prey and hosts (in the case of parasites) also follow daily rhythms [3,4,5]. How daily rhythms, whether they are clock-controlled or direct responses to rhythmic environmental cues, shape, and are shaped by interactions between organisms is poorly understood. We address this by examining the consequences of daily rhythms in the interactions between vectors, their hosts, and their parasites. Specifically, we ask how the time-of-day that mosquitoes blood feed combines with the timing (phase) of rhythms in hosts and with malaria infection to shape vector fitness and disease transmission. Given reports that some mosquito populations have altered the time-of-day they bite (likely in response to the use of insecticide-treated bed nets) [6,7,8,9,10,11,12,13], exploring the consequences of perturbed blood foraging rhythms for mosquito fitness and malaria transmission is urgently required.
Mosquitoes exhibit periodicity in many fitness determining activities, including sugar feeding, the formation of mating swarms, insecticide resistance and blood-feeding [14, 15]. In keeping with this, ~ 20% of the Anopheles gambiae genome is expressed in patterns following daily rhythms . Thus, the circadian clock enables mosquitoes to coordinate the timing of the physiological, cellular and molecular processes that underpin behaviours, with rhythms in the abiotic environment and/or other internal processes [2, 17]. For example, Anopheline mosquitoes are primarily night-biters [15, 18, 19] and processes associated with being active and foraging at night, including glycolysis, energy sensing and nutrient mobilization are upregulated in concert [16, 20]. Many genes, however, are not clock-regulated but still follow daily rhythms (including some An. gambiae odorant-binding proteins) and are driven by a direct response to light or dark . Indeed in both An. gambiae and Aedes aegypti, more rhythmic genes are detected under light:dark conditions than dark:dark conditions [21, 22].
A key benefit of clock-control is that organisms can anticipate dawn/dusk and prepare in advance by up- or downregulating physiological processes. For example, processes required to cope with a blood meal are upregulated in the mosquito’s active phase (night time for Anopheles sp.) [14, 16]. This includes catalase and other factors used to detoxify reactive oxygen species (ROS) generated as a product of blood (heme) digestion, and members of the V-ATPase complex which drive water excretion to minimise the 3-fold increase in volume that a blood meal brings [23, 24]. Exposure to ROS increases mortality and reduces clutch size of mosquitoes [23, 25, 26]. Further, as a consequence of the detoxification of blood meal induced ROS, there is a proliferation of mosquito gut microbiota  which have complex interactions with parasite infection  that may vary in line with time-of-day a blood meal is taken. In addition to rhythms in processes associated with foraging, the activities and locations of immune effectors cycle throughout the day. For example, immune defences are upregulated during the day in diurnal insects, such as Drosophila [29, 30]. Whether immune defences peak at night in nocturnal mosquitoes is unknown but some immune genes implicated in interactions with malaria parasites are expressed with circadian rhythms . How circadian rhythms in insect immune defences relate to protection from infection or the severity of disease is unclear. For instance, Drosophila challenged with Pseudomonas aeruginosa at night are more likely to survive the infection than those challenged in the day. However, perturbation of clock genes to generate arrhythmic mutant flies can result in both decreased survival or enhanced survival depending on the specific genes modified . Further, there are complex consequences of challenging An. stephensi with E. coli or the malaria parasite P. chabaudi at different times of day [32, 33].
Given the potential for circadian rhythms to influence the ability of mosquitoes to cope with a blood meal and with parasites, the time-of-day that mosquitoes forage has implications for both mosquito fitness and disease transmission. These consequences are likely to be complex . If feeding in the daytime means that mosquitoes are less able to cope with the osmotic and oxidative costs of blood, their fecundity and survival should suffer. Indeed, mosquitoes in poor condition as a consequence of feeding in the day may have compromised immune defence and this might explain recent observations that day-fed An. stephensi harbour higher densities of P. chabaudi than night-fed mosquitoes (although parasite rhythms also mediate this effect) . Alternatively, ROS is a key player in insect immune responses and so, if day-fed mosquitoes do not manage their ROS efficiently, they may suffer collateral damage but also benefit from enhanced parasite defence. Furthermore, it is also necessary to recognise that mosquitoes feed on hosts that have their own circadian rhythms . This includes rhythms in red blood cell composition and density, hematocrit, amino acid composition and immune effectors [34,35,36,37,38,39]. Thus, rhythms in the composition of mammalian blood could exacerbate (or reduce) the effects of a daytime blood meal on mosquito survival and fecundity.
Clearly, predicting the net effects of how host rhythms and vector rhythms interact to shape malaria transmission is challenging but important. Such interactions could shape the probability and intensity of infection in mosquitoes as well as mosquito population dynamics. Here, three experiments are described that probe the consequences, under a variety of scenarios, of time-of-day-specific blood-feeding for proxies estimating the fitness of mosquitoes and malaria parasites. The aims are to determine: (i) if the timing of a blood meal affects mosquito survival and fecundity; (ii) whether the effects of time-of-day are exacerbated by other characteristics of host blood or malaria infection; and (iii) the consequences of blood-feeding at different times of day for malaria transmission.
All experiments examine metrics of mosquito fecundity and lifespan in response to perturbing the time-of-day (morning) or (evening) that mosquitoes receive a blood meal, but differ in the following respects. The first experiment (“blood quality and host time”, Fig. 1a) includes the effects of both host time-of-day and feeding on blood from anaemic versus control mice. To further probe a role for blood quality, the second experiment (“blood quality”, Fig. 1b) uses a different approach to examine the effects of feeding on anaemic blood but does not consider host time-of-day. The third experiment (“infection,” Fig. 1c) focuses on Plasmodium berghei infection of mosquitoes.
For all experiments, hosts were 10–12-week-old MF1 male mice housed at 21 °C with ad libitum food and drinking water supplemented with 0.05% para-aminobenzoic acid (to supplement parasite growth). Mice were housed in groups of five in either 12:12 light:dark (LD; lights on at 07:00 GMT, lights off at 19:00 GMT) or inverted dark:light photocycle (DL; lights on at 19:00 GMT, lights off at 07:00 GMT) depending on the experiment. Mice were entrained to their respective light schedules for at least 21 days prior to mosquito blood feeds. Prior to donating a blood meal, each mouse was anaesthetized (17% Dormitor, 13% Vetelar, 70% PBS administered at 4 µl/g) and then exposed to a single cage of mosquitoes.
All Anopheles stephensi mosquitoes were maintained under standard insectary conditions of 27 ± 1 °C, 70% relative humidity and a 12:12 light:dark photocycle, with lights on at 07:00 GMT (ZT0) and lights off at 19:00 GMT (ZT12) (ZT0, Zeitgeber Time 0, is defined as time of lights on). Larvae were reared at a density of ~ 250 larvae per 1.5 l of distilled water. Between 12 and 14 days after hatching, pupae were transferred to emergence cages in incubators (27 ± 1 °C, 60 ± 5% relative humidity) with one-hour light ramping to simulate a dawn (starting at 07:00 GMT; ZT0) and dusk (19:00 GMT; ZT12). Mosquitoes were supplied with ad libitum access to 10% fructose solution supplemented with 0.05% paraminobenzoic acid. In the second experiment only, mosquitoes were treated with antibiotics (0.05% gentamicin) administered via their fructose solution 4–5 days before blood meals. For all experiments, female mosquitoes were randomly selected from 3–4 emergence cages, transferred to 2 l holding cages and starved of fructose solution for 24 h before their blood meals. Cages contained 15–85 mosquitoes (depending on the sampling regime of each experiment). Regardless of mosquito number, all mosquitoes were able to blood feed until satiated. For all feeds, each cage of females was exposed to an anaesthetized mouse for 30 min in a light setting that matched the mosquito time-of-day (i.e. morning-fed mosquitoes were fed during lights on and evening feeds were performed under dim red light). Unfed females were removed from the cages (< 5 per cage in all cases). After feeding, mosquitoes were housed in incubators at temperatures of either 20.5 or 26.0 °C (± 0.5 °C), depending on the experiment.
Experiment 1: blood quality and host time
Mosquito cages were randomly assigned to receive a blood meal in their morning 09:00 GMT (ZT2) or evening 21:00 GMT (ZT14). These feed times are analogous to the mosquito resting period (morning) or active period (evening) as evident from wild caught and laboratory-based studies (Fig. 2, [40,41,42]). Within each feeding time, cages were allocated to a further four groups, based on host treatment (anaemic or control mice) and host time-of-day [morning mice (ZT2) or evening mice (ZT14)]. The availability of mice experiencing their morning or evening to feed to mosquitoes in their morning or evening was achieved by housing mice in room with LD and DL lighting schedules. This resulted in an experiment with a 2 × 2 × 2 design: eight groups varying by feed time (morning/evening), host blood treatment (anaemic/control), and host time (morning/evening) (Fig. 1a). Note, this is the only experiment that perturbs host time-of-day.
Anaemia was induced in half of the mice by intraperitoneal injection of 125 mg/kg of phenylhydrazine 3 days before feeding to mosquitoes. The control mice received a sham injection of 100 µl PBS. On the day of feeding, red blood cell (RBC) counts (×109 ml−1) for control mice (7.48 ± 0.13 SE) were almost 2-fold higher than for phenylhydrazine treated hosts (3.80 ± 0.12 SE; t = 21.27, df = 45.67, P < 0.001). Each cage contained 50 mosquitoes and each of the 8 treatment groups contained 6 cages. On day 2 post-blood meal (PBM), mosquitoes were allocated to individual housing (50 ml falcon tubes with ad libitum access to 10% glucose solution via a 1.5 ml microcentrifuge tube feeder). Fecundity and mortality were tracked for 20 mosquitoes from each cage (960 total). Each female was given a 30 mm diameter Petri dish lined with filter paper and 3 mm depth of distilled water as an oviposition habitat. Mosquitoes were checked daily and if eggs were present, the egg dish was replaced. Egg bowls were photographed at the time of removal (for clutch size counts) incubated for 6 days and then photographed again so that all hatched larvae (alive and dead) could be counted (to estimate hatch rate). For all females (that did or did not lay) egg bowls were removed on day 9 PBM.
Additionally, the volume and density of blood meals were estimated for 10 randomly chosen mosquitoes from each cage (480 total) 2 hours after their blood meal. The right wing of each mosquito was photographed, and the abdomen removed and homogenised in 500 µl drabkins solution for ~ 30 min . Samples were split into two 200 µl sub-samples and optical density (OD) read by a spectrophotometer at 540 nm (each mosquito was read in duplicate, and an average taken). To generate control series for each cage, 8 µl of blood was removed from each mouse used to feed mosquitoes at the time of feeding and used to generate 4 µl, 1 µl, 0.8 µl and 0.4 µl standards. Host RBC density readings (cells per µl) were also obtained at the time of feeding to calculate the RBC density of the blood meal. Wing length was obtained from the photographs, converted to mm and used to control for any potential differences in blood meal volume and density due to variation in body size (using the software package ImageJ ).
Experiment 2: blood quality
Here, instead of phenylhydrazine treatment, blood quality was perturbed by using malaria infection to generate anaemia. Mosquito cages were randomly assigned to morning 09:00 GMT (ZT2) or evening 21:00 GMT (ZT14) feed times. At each feeding time, half the cages were exposed to anaemic or (uninfected) control mice. This resulted in an experiment with a 2 × 2 design: four groups varying by the timing of their blood meal (morning/evening) and blood treatment (anaemic/control) (Fig. 1b). Note, host time-of-day was standardised by housing mice in two rooms with inverted light schedules (DL and LD), enabling both the morning- and evening-fed mosquitoes to feed on hosts experiencing their evening (host ZT14). Six cages were fed, at each time point, on control mice and nine cages, at each time point, on anaemic mice. Each cage exposed to control mice contained 15 mosquitoes and each cage exposed to anaemic mice contained 20 mosquitoes. Mortality was tracked as for Experiment 1 (but for 10 individuals per cage; 300 total) and egg dishes were provided until day 14 PBM.
All feeds occurred on mice at day 11 post-infection (PI) after infection with 1 × 106 P. chabaudi CR parasitized RBCs or sham infection (controls; 100 µl PBS). Plasmodium chabaudi has a synchronous asexual cycle so donor mice were used from each room (DL and LD) to ensure that all hosts were infected with rings (i.e. parasite and host rhythms were phase matched; ). By day 11 PI, significant anaemia had occurred (mean RBC density × 109 ml−1: Control = 7.88 (± 0.16 SE), anaemic = 4.44 (± 0.10 SE); t = 18.77, df = 19.28, P < 0.001) and hosts were mounting strong immune responses, so the parasite was not able to establish an infection in mosquitoes (parasite mating is very vulnerable to suboptimal conditions in the blood meal ). Thus, mosquitoes received poor quality blood as a result of a more ecologically realistic perturbation than PHZ, without the confounding effects of becoming infected themselves. This was verified by examining 10 randomly selected mosquitoes from each cage exposed to anaemic mice on day 14 PBM. Specifically, the midgut of each mosquito was dissected, stained for two minutes in 0.5% mercurochrome, washed in PBS and total oocysts per midgut were counted via microscopy. No oocysts were detected.
Experiment 3: infection
Mosquito cages were randomly assigned to four groups. Two groups received their blood meal in the morning 10:00 GMT (morning, ZT3) and the others were fed in the evening at 20:00 GMT (evening, ZT13). At each feed time, half of the cages were exposed to Plasmodium berghei infected mice or naïve (uninfected) control mice. This resulted in an experiment with a 2 × 2 design: four groups varying by the timing of their blood meal (morning/evening) and blood treatment (infected/uninfected) (Fig. 1c). Note that time-of-day for parasites/hosts and mosquitoes is synonymous; morning-fed mosquitoes received blood from hosts also experiencing their morning, and vice versa for evening-fed mosquitoes. At each time point, six cages were fed on infected mice and four fed cages on uninfected mice.
All feeds occurred on mice at day 6 PI after inoculation with 1 × 105 P. berghei parasitized RBCs or sham infection (controls; 100 µl PBS). Infections (and sham injections) were staggered by 10 h to ensure that morning- and evening-fed mosquitoes were exposed to infections of the same age (144 h). Plasmodium berghei was chosen because its asexual cycle is asynchronous, ensuring that morning- and evening-fed mosquitoes did not receive significantly different stage distributions of asexual parasites (feed time:parasite stage; χ24 = 1.28, P = 0.29; Fig. 3a) or gametocyte densities/ages (mean gametocyte density × 107 ml−1: morning = 3.93 (± 0.14 SE), evening = 3.80 (± 0.1 SE); t = 0.77, df = 10, P = 0.46; Fig. 3b). On day 6 PI, P. berghei had not significantly reduced the RBC density of hosts (mean RBC density × 109 ml−1: Control = 8.17 (± 0.08 SE), Infected = 7.85 (± 0.18 SE); t = 1.64, df = 14.71, P = 0.12).
At the time of feeding, each cage contained 85 mosquitoes. After the blood meal, 15 mosquitoes from cages fed on infected mice were removed (180 total) and used to monitor oocyst prevalence and density as for Experiment 2. To track mosquito fecundity and mortality, a subset of 15 randomly selected females were removed from each cage (300 total) 2 days PBM and housed individually in 200 ml cups with ad libitum access to 10% fructose solution. On day 3 PBM, each female was given a 30 mm diameter Petri dish lined with filter paper and 3 mm depth of distilled water as an oviposition habitat. Mosquitoes were checked daily until death, if eggs were present the egg dish was replaced (up until day 21 PBM).
R version 2.6.1  was used for all analyses. Model simplification was carried out by stepwise deletion of the least significant term and only minimal models are reported. Measurements made from mice at the time of feeding (red blood cell counts and parasite stage composition and densities), and time-of-day differences in infection load for mosquitoes were analysed with Studentʼs t-test. Mosquito fecundity metrics, proportion of females that laid and hatch rate, were analysed using generalised linear mixed-effects models with binomial error structures. Clutch size and blood meal measures were analysed using linear mixed effect models. In both types of linear models, identity of the mosquito cage was included as a random effect. All models met model assumptions: independence of data points, normality of residuals and homogeneity of variances (confirmed through assessing the model plots, the Shapiro–Wilk test and Bartlett’s test). Cox proportional hazard models with mosquito identity nested within cage as random effects (frailty model) were used to estimate the effects of feed time and host blood manipulations on the time taken to lay and lifespan (coxme package in R ). All Cox models model met the proportional hazards assumptions based on Schoenfeld’s residuals (evaluated using the ‘cox.zph’ function R; P > 0.1 for all variables). Clutch size of mosquitoes that laid and its interactions with experimental treatments was also controlled for because the data indicated considerable heterogeneity in clutch size, and trade-offs between survival and reproduction have been reported [49, 50] and may depend on resource availability, which may vary as a consequence of perturbations of blood quality. For this reason, mosquitoes that did not lay eggs were excluded from time to lay, clutch size and lifespan analyses. For all analyses, main effects and two-way interactions were investigated.
We carried out three experiments to determine how the timing of receiving a blood meal affects aspects of mosquito survival and fecundity, and whether qualities of host blood or malaria infection modulate the effects of the time-of-day that mosquitoes feed.
Experiment 1: blood quality and host time
This experiment (Fig. 1a) recognises that hosts have circadian rhythms in blood composition and was designed to address if host time-of-day and blood quality (chemical induced anaemia) interact with mosquito feeding time-of-day to shape the following parameters (see Table 1 for a summary).
Blood meal: volume and density
There was no significant effect of feed time (χ27 = 1.02, P = 0.31), host time (χ25 = 0.01, P = 0.91), or their interaction (χ29 = 0.40, P = 0.53) on the volume of the blood meal. The effect of host blood quality was not significantly influenced by interactions with feed time (χ28 = 0.75, P = 0.39) or host time (χ26 = 2.29, P = 0.13). However, mosquitoes that fed on anaemic hosts took up a greater volume of blood than those that fed on control hosts (mean ± SE blood meal volume (µl) per mm wing length: control = 0.26 ± 0.01), anaemic = 0.33 ± 0.01; χ24 = 17.90, P < 0.0001; Fig. 4a). There was also a borderline significant interaction between host time and host blood quality on the RBC density of the blood meal (χ26 = 4.30, P = 0.038; Fig. 4b). Specifically, mosquitoes that fed on control hosts consumed more RBCs than those that fed on anaemic hosts, especially when fed on hosts that experienced their morning (mean ± SE × 106; control hosts: morning = 2.11 ± 0.94, evening = 1.77 ± 0.75; anaemic hosts: morning = 1.24 ± 0.54, evening = 1.29 ± 0.60). There was no significant effect of feed time (χ27 = 0.66, P = 0.42), nor its interactions with host blood quality (χ29 = 0.65, P = 0.42) or host time (χ28 = 3.25, P = 0.07) on the RBC of the blood meal.
Reproduction: proportion laid
Neither host blood quality (χ25 = 1.60, P = 0.21), host time (χ24 = 2.44, P = 0.12), or their interaction (χ28 = 0.02, P = 0.89) significantly affected the probability each mosquito laid. However, feed time did matter, with mosquitoes that fed in the morning more likely to lay than those that fed in the evening (mean ± SE proportion of females that laid: morning = 0.82 ± 0.02, evening = 0.65 ± 0.02; χ23 = 27.56, P < 0.0001; Fig. 5a). However, feed time did not significantly interact with either host blood quality (χ26 = 0.41, P = 0.52) or host time (χ27 = 0.05, P = 0.83).
Reproduction: time to lay
For mosquitoes that laid, neither host blood quality (z = 0.73, P = 0.47) or host time (z = 0.95, P = 0.34) influenced the time it took mosquitoes to lay eggs. Feed time did have an effect with mosquitoes that fed in the morning laying sooner than those that fed in the evening (mean ± SE days taken to lay since egg bowls were provided: morning = 1.15 ± 0.03, evening = 1.44 ± 0.04; evening:morning HR = 0.64 ± 0.08, z = − 5.35, P < 0.001; Fig. 5a).
Reproduction: clutch size
Clutch size was shaped by a borderline interaction between feed time and host blood quality (χ26 = 4.13, P = 0.042; Fig. 5b). Mosquitoes fed on control mice had higher clutch sizes than those that fed on anaemic hosts, and this difference was greatest when mosquitoes fed in the morning (mean ± SE clutch size: morning-fed: control hosts = 65.57 ± 1.77, anaemic hosts = 51.06 ± 1.70; evening-fed: control hosts = 61.88 ± 1.97, anaemic hosts = 55 ± 2.09). There was also a non-significant tendency for mosquitoes that fed on hosts experiencing their morning to have higher clutch size (mean ± SE clutch size: morning hosts = 56.36 ± 1.37, evening hosts = 59.99 ± 1.34; χ27 = 3.70, P = 0.054). However, this trend for an effect of host time was not modulated by feed time (χ28 = 3.06, P = 0.08) or host blood quality (χ29 = 0.03, P = 0.87).
Reproduction: hatch rate
Neither host blood quality (χ24 = 0.12, P = 0.73) or host time (χ26 = 0.02, P = 0.89) or their interaction (χ28 = 0.10, P = 0.75) influenced egg hatch rate. Likewise, there was no significant influence of feed time (χ23 = 1.31, P = 0.25) and its interactions with host blood quality (χ25 = 2.12, P = 0.15) and host time (χ27 = 0.40, P = 0.53). Mean hatch rate for all clutches was 0.69 (± 0.01 SE).
For mosquitoes that laid, neither host blood quality (z = 0.28, P = 0.78), host time (z = 0.75, P = 0.45) or feed time (z = − 0.10, P = 0.92) significantly influenced mortality rate (Fig. 5c–d). Clutch size was negatively associated with survival hazard (clutch HR = 0.996, z = − 2.54, P = 0.011), with smaller clutches (< 60 eggs) associated with a greater hazard than larger clutches (> 60 eggs). The median lifespan for all mosquitoes (that laid) was 13 days post-blood meal.
Experiment 2: blood quality
Experiment 1 suggested that blood quality and mosquito time-of-day of feeding shaped some mosquito reproductive measures (tendency to lay and clutch size). Experiment 2 (Fig. 1b) further investigated time-of-day of feeding and blood quality by using P. chabaudi malaria infection to generate anaemia (see Table 1 for a summary). Host time-of-day was not investigated further because Experiment 1 revealed that it did not significantly shape mosquito reproduction or lifespan (host time-of-day only remained in an interaction with borderline significance for blood meal density).
Reproduction: proportion laid
Neither host blood quality (χ24 = 0.73, P = 0.39), feed time (χ23 = 1.59, P = 0.21) nor their interaction (χ25 = 1.07, P = 0.30) significantly influenced the proportion of females that laid (Fig. 6a). The mean proportion of females that laid per cage was 0.48 (± 0.04 SE).
Reproduction: time to lay
For mosquitoes that laid, neither host blood treatment (z = 0.52, P = 0.60), feed time (z = 0.99, P = 0.32) nor their interaction (z = 0.74, P = 0.46) significantly affected the time taken to lay (Fig. 6a). The average number of days to lay since eggs bowls were provided was 2.63 (± 0.17 SE).
Reproduction: clutch size
Host blood quality significantly affected clutch size, with mosquitoes fed on control blood laying larger clutches than mosquitoes that received anaemic blood (mean ± SE clutch size: control hosts = 90.36 ± 5.06, anaemic hosts = 66.41 ± 3.06; χ24 = 8.62, P = 0.003; Fig. 6b). Feed time did not influence clutch size (χ25 = 1.84, P = 0.17) or modulate the effect of blood quality (χ26 = 1.81, P = 0.18).
For mosquitoes that laid, neither host blood quality (z = 1.05, P = 0.29), feed time (z = − 0.98, P = 0.33) or clutch size (z = − 1.81, P = 0.07) influenced mortality rates (Fig. 6c). The median lifespan was 39 days post-blood meal.
Experiment 3: infection
Having investigated blood quality and host time-of-day in the previous experiments, we switched focus to consider the effects of malaria infection by feeding mosquitoes on blood with infectious P. berghei parasites at different times of day (Fig. 1c and Table 1). In addition to the effects on mosquito reproduction and lifespan, the performance of parasites was also examined.
No mosquitoes that fed on control hosts became infected but 94 (± 2%) mosquitoes that fed on infected hosts contained oocysts. Within mosquitoes fed on infected hosts, feed time did not influence infection prevalence (t(10) = 0.598, P = 0.56; Fig. 7a) or the intensity of infection (mean ± SE oocysts = 171.3 ± 9.84; t(178) = − 1.442, P = 0.15; Fig. 7b).
Reproduction: proportion laid
A significantly greater proportion of uninfected than infected mosquitoes laid eggs (mean ± SE proportion laid: uninfected = 0.59 ± 0.04, infected = 0.44 ± 0.04; χ23 = 5.44, P = 0.0197; Fig. 8a). The influence of infection was not modulated by feed time (χ25 = 0.009, P = 0.93) but there was a trend in which mosquitoes fed in the morning were more likely to lay (mean ± SE morning = 0.56 ± 0.04, evening = 0.44 ± 0.05; χ24 = 3.66, P = 0.056; Fig. 8a).
Reproduction: time to lay
For mosquitoes that laid, those that were infected laid two days sooner than uninfected individuals and at any time point, were ~ 70% more likely to lay (mean ± SE days taken to lay since egg bowls were provided: uninfected = 6.88 ± 0.48, infected = 5.05 ± 0.35; infected:uninfected Hazard Ratio (HR) = 1.71 (± 0.171, z = 3.12, P = 0.002; Fig. 8a). Neither the interaction between infection status and feed time (z = 0.28, P = 0.78) nor feed time alone (z = − 1.82, P = 0.07) influenced time to lay.
Reproduction: clutch size
Neither host blood treatment (χ24 = 0.59, P = 0.44), feed time (χ25 = 0.07, P = 0.79) nor their interaction (χ26 = 0.30, P = 0.59) significantly influenced clutch size (Fig. 8b). Females laid an average of 98 (± 2.6 SE) eggs per clutch.
For mosquitoes that laid, infection had a negative effect on lifespan with infected mosquitoes dying sooner than uninfected mosquitoes (median lifespan: infected = 21 days, uninfected = 24 days). Further, infected mosquitoes had a ~ 50% higher overall hazard of dying (infected:uninfected HR = 1.53 (± 0.178 SE), z = 2.4, P = 0.016; Fig. 8c). Neither feed time (z = − 0.98, P = 0.33) nor clutch size (z = − 0.56, P = 0.57) influenced lifespan.
Here, we examine whether the fitness of female An. stephensi mosquitoes is affected by the time-of-day they receive a blood meal, either directly or through interactions with perturbations of blood quality and malaria infection. Specifically, we compared mosquitoes fed on control or anaemic hosts (using two different manipulations of anaemia) in which host time-of-day also varied, and whether mosquitoes were uninfected or infected with P. berghei malaria. The results of the three experiments are summarised in Table 2 from the perspective of the mosquitoes’ time-of-day of feeding. Overall, we found few effects of time-of-day of feeding. First, morning-fed mosquitoes appeared > 25% more likely to lay than evening-fed (Figs. 5a, 6a, 8a). Secondly, in response to chemically induced anaemia, morning-fed mosquitoes laid 0.3 of a day sooner (Fig. 5a) and produced ~ 15 (30%) more eggs than evening-fed mosquitoes (Fig. 5b). However, time-of-day of feeding did not substantially influence longevity of mosquitoes or the prevalence and intensity of P. berghei infection (Fig. 7). We also found mixed results for the other variables manipulated in the experiments. Host time-of-day did not influence any of the mosquito fitness metrics we measured. The effects of blood quality were similar across both of the experiments in which it was perturbed (Experiments 1 and 2); only clutch size varied in response, in which mosquitoes fed on anaemic blood laid ~ 20 (~ 22%) fewer eggs (Figs. 5b, 6b). Infection status also correlated with fitness metrics; infected mosquitoes had shorter lifespans (21 days post-blood meal for infected vs 24 days for uninfected; Fig. 8c), were less likely to lay eggs (44% vs 60% laid) and laid sooner (~2 days) than uninfected mosquitoes (Fig. 8a).
We expected that mosquitoes receiving a blood meal at an unexpected time-of-day (i.e. morning) would experience fitness costs in the form of reduced lifespan and/or loss of reproduction. However, we found no effect on lifespan and the effects on reproduction were not consistent with costs; a higher probability of laying eggs and a modest increase in fecundity appear to be fitness benefits from morning feeding (Table 2). Laying sooner may be a fitness cost if it results in poor quality eggs or trade-off against immune defence . However, we found no evidence of a quantity-quality reproductive trade-off because eggs from females in all groups (Experiment 1) hatched at a similar rate. In Experiments 2 and 3 we saw little difference between morning- and evening-fed mosquitoes in the time taken to lay despite the 10-hour ‘head-start’ of morning-fed mosquitoes. If egg maturation takes a fixed window of time since feeding, this suggests morning-fed mosquitoes are deliberately delaying their oviposition or waiting until the next ‘gate’ to oviposit if oviposition is clock-controlled. Mark-recapture studies with wild An. farauti show that an earlier feed time is associated with irregularities in oviposition cycle, sometimes lengthening or shortening by a day [52,53,54]. This demonstrates flexibility in the day of oviposition post-blood meal, but whether there is additional flexibility for time-of-day requires further investigation [55, 56]. Given daily mortality risk for mosquitoes (estimated to be around 10% for An. gambiae ), intuition suggests it would be adaptive to lay as soon as they are able.
A lack of costs of morning feeding could have several non-mutually exclusive explanations. First, costs were expected because the expression of numerous genes involved in processes required to neutralise the ROS produced by blood digestion is rhythmic . However, transcriptional circadian phases do not always reflect protein abundance rhythms [58, 59]. Nine of the 12 V-ATPase subunits of the vesicular type H+ ATPase (V-ATPase), which is associated with maintaining osmotic balance during the increase in volume resulting from a blood meal, are rhythmic at the protein level (peaking at dusk in An. gambiae ). This has led to the suggestion that water excretion is compromised in mosquitoes feeding in the daytime and so, they should compensate by taking smaller blood meals . However, we found no evidence of feeding time-of-day affecting blood meal volume or density. Secondly, immune responses are suggested to be timed to defend against pathogens acquired during foraging . However, for mosquitoes, there may be an acute need for immune control of the proliferation of gut microbiota that expand upon an influx of blood . ROS favours pathogen defence and a combination of digestion-related and immune-related ROS might erode rhythmicity in ROS levels, or defences may be upregulated as a direct response to feeding, rather than in a time-of-day dependent manner. Thirdly, when only comparing two time points on a symmetrical curve, there is a risk of picking the same intercept as the curve ascends and descends (“shoulder problem”). However, this is unlikely to be the case in our experiments because mosquitoes were in their rest phase in morning feeds and their active phase in evening feeds . Fourthly, if feeding at the wrong time-of-day has only minor negative fitness consequences, manipulating feeding time-of-day over multiple blood-feeding and oviposition cycles might be required to detect costs, or keeping mosquitoes in a more stressful and ecologically realistic manner.
Many nutrients and amino acids in the blood that are essential to mosquito egg development (e.g. isoleucine)  exhibit circadian periodicity [34,35,36] but we found no evidence that host time-of-day matters for mosquitoes feeding on either healthy or anaemic mice. Mice take their largest meal around lights off, and so, by carrying out feeds on mice several hours into their active versus rest phases the difference in blood meal composition due to metabolic processes should have been considerable. Perhaps these factors are not limiting at any point in their rhythms, especially for mosquitoes receiving blood from well-fed laboratory mice. Further work could consider investigating the role host time-of-day in more dramatic manipulations of blood composition, for example, during infection and under food-limited conditions.
Our perturbations of anaemia did affect mosquito reproduction; clutch size was reduced in mosquitoes feeding on anaemic blood. Inducing anaemia with phenylhydrazine causes oxidative damage to red blood cells which are then cleared from circulation . ROS damages mosquitoes [23, 25, 26], but by feeding mosquitoes three days after phenylhydrazine administration, the ROS it causes should have been neutralised. Thus, the main difference between blood from control and phenylhydrazine-treated mice is the age structure and density of RBC. Our data suggest that mosquitoes take up a larger volume of blood from phenylhydrazine-treated mice (perhaps facilitated by lower viscosity of anaemic blood ), but that this does not fully compensate and equalise blood meal RBC densities to those from feeds on control mice (Fig. 4). There may be additional differences in blood quality between chemical- and infection-induced anaemia. However, given their similar impacts, the ability to garner fewer resources from anaemic blood could explain the reduction in clutch size we observed. This is supported by previously revealed positive correlations between haematin content of blood and clutch size [65, 66]. Additionally, we found that hatch rate is a decreasing function with lay day (χ25 = 12.58, P < 0.001) but only in those mosquitoes that fed on anaemic hosts. This result is similar to that reported in infected mosquitoes  suggesting that this result may be an effect of blood quality rather than parasite infection.
Our results contrast with recent work showing that mosquitoesʼ blood-feeding in the daytime are more likely to become infected after feeding on P. chabaudi infected mice, although P. chabaudi oocyst burdens did not differ between feed times . Compared to P. berghei, P. chabaudi generally transmits with far lower prevalence and burden, which may facilitate detection of subtle time-of-day effects. An alternative possibility is that mosquito time-of-day effects are driven by an interaction with parasite time-of-day and so, are only observed in infections with synchronously developing parasites such as P. chabaudi (in which a specific age of gametocytes is present in blood meals), or in asynchronous species such as P. relictum in which parasite abundance in the blood (rather than age) is rhythmic . In contrast to the effects of our other perturbations in the experiments presented here, we found negative effects of infection on lifespan. Costs of malaria infection on mosquito lifespan have been observed in other malaria model systems (reviewed in ) as well as an advancement of egg laying . The advanced laying of infected mosquitoes may be a form of terminal investment because organisms with low survival prospects rush to reproduce before dying [70,71,72,73]. If our mosquitoes adopted terminal investment, it is necessary to explain why uninfected mosquitoes do not benefit from early reproduction. This could be because advancing reproduction also results in reduced clutch size (but we did not observe this), lower hatch rate (Experiment 1 suggests this does not occur either), trade-offs against anti-parasite immune responses, or reduces the probability or size of future clutches . Alternatively, mosquitoes may restrict essential lipid resources available to parasites by allocating them to eggs as quickly as possible  or since mounting an immune response is costly to fecundity, laying early may be a compromise for both fecundity and survival .
In summary, we found that taking a blood meal in the morning compared to the evening has no, or minor negative, effects on the fitness of mosquitoes, nor impacts upon on P. berghei malaria infection. If our results from a laboratory colony of mosquitoes living in benign conditions hold for wild mosquitoes, it suggests that mosquitoes have sufficient flexibility in their physiology to cope with changes in biting time induced by evading insecticide-treated bed nets. Future work should consider the impact of multiple feeding cycles and the abiotic stresses imposed by the need to forage for blood when hosts are not protected by bed nets.
Availability of data and materials
The datasets supporting the conclusions of this article are available in the Edinburgh DataShare repository, https://doi.org/10.7488/ds/2485.
Edgar RS, Green EW, Zhao Y, van Ooijen G, Olmedo M, Qin X, et al. Peroxiredoxins are conserved markers of circadian rhythms. Nature. 2012;485:459–64.
Vaze KM, Sharma VK. On the adaptive significance of circadian clocks for their owners. Chronobiol Int. 2013;30:413–33.
Reece SE, Prior KF, Mideo N. The life and times of parasites: rhythms in strategies for within-host survival and between-host transmission. J Biol Rhythms. 2017;32:516–33.
Martinez-Bakker M, Helm B. The influence of biological rhythms on host-parasite interactions. Trends Ecol Evol. 2015;30:314–26.
Parecis-Silva PV, Nuvoloni FM, Feres RJ. Day vs. night: the importance of the circadian cycle over metacommunities and predator–prey densities. Int J Acarol. 2016;42:141–8.
Wamae PM, Githeko AK, Otieno GO, Kabiru EW, Duombia SO. Early biting of the Anopheles gambiae s.s. and its challenges to vector control using insecticide treated nets in western Kenya highlands. Acta Trop. 2015;150:136–42.
Cooke MK, Kahindi SC, Oriango RM, Owaga C, Ayoma E, Mabuka D, et al. ‛A bite before bedʼ: exposure to malaria vectors outside the times of net use in the highlands of western Kenya. Malar J. 2015;14:259.
Sougoufara S, Diedhiou SM, Doucoure S, Diagne N, Sembene PM, Harry M, et al. Biting by Anopheles funestus in broad daylight after use of long-lasting insecticidal nets: a new challenge to malaria elimination. Malar J. 2014;13:125.
Yohannes M, Boelee E. Early biting rhythm in the afro-tropical vector of malaria, Anopheles arabiensis, and challenges for its control in Ethiopia. Med Vet Entomol. 2012;26:103–5.
Moiroux N, Gomez MB, Pennetier C, Elanga E, Djenontin A, Chandre F, et al. Changes in Anopheles funestus biting behavior following universal coverage of long-lasting insecticidal nets in Benin. J Infect Dis. 2012;206:1622–9.
Russell TL, Govella NJ, Azizi S, Drakeley CJ, Kachur SP, Killeen GF. Increased proportions of outdoor feeding among residual malaria vector populations following increased use of insecticide-treated nets in rural Tanzania. Malar J. 2011;10:80.
Taylor B. Changes in feeding behavior of a malaria vector, Anopheles farauti Lav, following use of DDT as a residual spray in houses in British Solomon-Islands Protectorate. Trans R Entomol Soc Lond. 1975;127:277–92.
Takken W. Do insecticide-treated bednets have an effect on malaria vectors? Trop Med Int Health. 2002;7:1022–30.
Rund SSC, O’Donnell AJ, Gentile JE, Reece SE. Daily rhythms in mosquitoes and their consequences for malaria transmission. Insects. 2016;7:14.
Duffield GE, Acri DJ, George GF, Sheppard AD, Beebe NW, Ritchie SA, et al. Diel flight activity of wild-caught Anopheles farauti (s.s.) and An. hinesorum malaria mosquitoes from northern Queensland, Australia. Parasit Vectors. 2019;12:48.
Rund SSC, Hou TY, Ward SM, Collins FH, Duffield GE. Genome-wide profiling of diel and circadian gene expression in the malaria vector Anopheles gambiae. Proc Natl Acad Sci USA. 2011;108:E421–30.
Sharma VK. Adaptive significance of circadian clocks. Chronobiol Int. 2003;20:901–19.
Bockarie MJ, Alexander N, Bockarie F, Ibam E, Barnish G, Alpers M. The late biting habit of parous Anopheles mosquitoes and pre-bedtime exposure of humans to infective female mosquitoes. Trans R Soc Trop Med Hyg. 1996;90:23–5.
Rund SS, Bonar NA, Champion MM, Ghazi JP, Houk CM, Leming MT, et al. Daily rhythms in antennal protein and olfactory sensitivity in the malaria mosquito Anopheles gambiae. Sci Rep. 2013;3:2494.
Balmert NJ, Rund SSC, Ghazi JP, Zhou P, Duffield GE. Time-of-day specific changes in metabolic detoxification and insecticide resistance in the malaria mosquito Anopheles gambiae. J Insect Physiol. 2014;64:30–9.
Rund SS, Gentile JE, Duffield GE. Extensive circadian and light regulation of the transcriptome in the malaria mosquito Anopheles gambiae. BMC Genom. 2013;14:218.
Leming MT, Rund SS, Behura SK, Duffield GE, O’Tousa JE. A database of circadian and diel rhythmic gene expression in the yellow fever mosquito Aedes aegypti. BMC Genom. 2014;15:1128.
Graca-Souza AV, Maya-Monteiro C, Paiva-Silva GO, Braz GRC, Paes MC, Sorgine MHF, et al. Adaptations against heme toxicity in blood-feeding arthropods. Insect Biochem Mol Biol. 2006;36:322–35.
Williams JC, Hagedorn HH, Beyenbach KW. Dynamic changes in flow-rate and composition of urine during the post-bloodmeal diuresis in Aedes aegypti (L). J Comp Physiol. 1983;153:257–65.
DeJong RJ, Miller LM, Molina-Cruz A, Gupta L, Kumar S, Barillas-Mury C. Reactive oxygen species detoxification by catalase is a major determinant of fecundity in the mosquito Anopheles gambiae. Proc Natl Acad Sci USA. 2007;104:2121–6.
Magalhaes T, Brackney DE, Beier JC, Foy BD. Silencing an Anopheles gambiae catalase and sulfhydryl oxidase increases mosquito mortality after a blood meal. Arch Insect Biochem Physiol. 2008;68:134–43.
Oliveira JH, Goncalves RL, Lara FA, Dias FA, Gandara AC, Menna-Barreto RF, et al. Blood meal-derived heme decreases ROS levels in the midgut of Aedes aegypti and allows proliferation of intestinal microbiota. PLoS Pathog. 2011;7:e1001320.
Dong Y, Manfredini F, Dimopoulos G. Implication of the mosquito midgut microbiota in the defense against malaria parasites. PLoS Pathog. 2009;5:e1000423.
McDonald MJ, Rosbash M. Microarray analysis and organization of circadian gene expression in Drosophila. Cell. 2001;107:567–78.
Stone EF, Fulton BO, Ayres JS, Pham LN, Ziauddin J, Shirasu-Hiza MM. The circadian clock protein timeless regulates phagocytosis of bacteria in Drosophila. PLoS Pathog. 2012;8:e1002445.
Lee JE, Ederyl I. Circadian regulation in the ability of Drosophila to combat pathogenic infections. Curr Biol. 2008;18:195–9.
Murdock CC, Moller-Jacobs LL, Thomas MB. Complex environmental drivers of immunity and resistance in malaria mosquitoes. Proc R Soc B Biol Sci. 2013;280:20132030.
Schneider P, Rund SSC, Smith NL, Prior KF, O’Donnell AJ, Reece SE. Adaptive periodicity in the infectivity of malaria gametocytes to mosquitoes. Proc R Soc B Biol Sci. 2018;285:20181876.
Feigin RD, Dangerfield HG, Beisel WR. Circadian periodicity of blood amino-acids in normal and adrenalectomized mice. Nature. 1969;221:94–5.
Fernstrom JD, Larin F, Wurtman RJ. Daily variations in the concentrations of individual amino acids in rat plasma. Life Sci I. 1971;10:813–9.
Wurtman RJ, Rose CM, Chou C, Larin FF. Daily rhythms in the concentrations of various amino acids in human plasma. N Engl J Med. 1968;279:171–5.
Clark RH, Korst DR. Circadian periodicity of bone marrow mitotic activity and reticulocyte counts in rats and mice. Science. 1969;166:236–7.
Scheiermann C, Kunisaki Y, Frenette PS. Circadian control of the immune system. Nat Rev Immunol. 2013;13:190–8.
Touitou Y, Touitou C, Bogdan A, Reinberg A, Auzeby A, Beck H, et al. Differences between young and elderly subjects in seasonal and circadian variations of total plasma proteins and blood volume as reflected by hemoglobin, hematocrit, and erythrocyte counts. Clin Chem. 1986;32:801–4.
Korgaonkar NS, Kumar A, Yadav RS, Kabadi D, Dash AP. Mosquito biting activity on humans & detection of Plasmodium falciparum infection in Anopheles stephensi in Goa, India. Indian J Med Res. 2012;135:120–6.
Reisen W, Aslamkhan M. Biting rhythms of some Pakistan mosquitoes (Diptera: Culicidae). Bull Entomol Res. 1978;68:313–30.
Rowland M, Boersma E. Changes in the spontaneous flight activity of the mosquito Anopheles stephensi by parasitization with the rodent malaria Plasmodium yoelii. Parasitology. 1988;97:221–7.
Briegel H, Lea AO, Klowden MJ. Hemoglobinometry as a method for measuring blood meal sizes of mosquitoes (Diptera: Culicidae). J Med Entomol. 1979;15:235–8.
Rueden CT, Schindelin J, Hiner MC, DeZonia BE, Walter AE, Arena ET, et al. Image J2: ImageJ for the next generation of scientific image data. BMC Bioinform. 2017;18:529.
OʼDonnell AJ, Schneider P, McWatters HG, Reece SE. Fitness costs of disrupting circadian rhythms in malaria parasites. Proc Biol Sci. 2011;278:2429–36.
Ramiro RS, Alpedrinha J, Carter L, Gardner A, Reece SE. Sex and death: the effects of innate immune factors on the sexual reproduction of malaria parasites. PLoS Pathog. 2011;7:e1001309.
R Development Core Team. R: a language and environment for statistical computing. Vienna: R Foundation for Statistical Computing; 2018. https://www.R-project.org/.
Therneau TM. coxme: mixed effects Cox models. R package version 2.2-10. 2018. https://cran.r-project.org/web/packages/coxme/index.html. Accessed 3 Dec 2018.
Hurd H. Evolutionary drivers of parasite-induced changes in insect life-history traits from theory to underlying mechanisms. Adv Parasitol. 2009;68:85–110.
Vezilier J, Nicot A, Gandon S, Rivero A. Plasmodium infection decreases fecundity and increases survival of mosquitoes. Proc Biol Sci. 2012;279:4033–41.
Ahmed AM, Hurd H. Immune stimulation and malaria infection impose reproductive costs in Anopheles gambiae via follicular apoptosis. Microbes Infect. 2006;8:308–15.
Charlwood J, Graves P, Marshall TDC. Evidence for a ‘memorized’ home range in Anopheles farauti females from Papua New Guinea. Med Vet Entomol. 1988;2:101–8.
Charlwood JD, Graves PM. The effect of permethrin-impregnated bednets on a population of Anopheles farauti in coastal Papua New Guinea. Med Vet Entomol. 1987;1:319–27.
Hii JL, Birley MH, Kanai L, Foligeli A, Wagner J. Comparative effects of permethrin-impregnated bednets and DDT house spraying on survival rates and oviposition interval of Anopheles farauti No. 1 (Diptera:Culicidae) in Solomon Islands. Ann Trop Med Parasitol. 1995;89:521–9.
Fritz ML, Huang J, Walker ED, Bayoh MN, Vulule J, Miller JR. Ovipositional periodicity of caged Anopheles gambiae individuals. J Circadian Rhythms. 2008;6:2.
McCrae AW. Oviposition by African malaria vector mosquitoes. I. Temporal activity patterns of caged, wild-caught, freshwater Anopheles gambiae Giles sensu lato. Ann Trop Med Parasitol. 1983;77:615–25.
Arifin SM, Zhou Y, Davis GJ, Gentile JE, Madey GR, Collins FH. An agent-based model of the population dynamics of Anopheles gambiae. Malar J. 2014;13:424.
Lee C, Etchegaray JP, Cagampang FR, Loudon AS, Reppert SM. Posttranslational mechanisms regulate the mammalian circadian clock. Cell. 2001;107:855–67.
Reddy AB, Karp NA, Maywood ES, Sage EA, Deery M, O’Neill JS, et al. Circadian orchestration of the hepatic proteome. Curr Biol. 2006;16:1107–15.
Shirasu-Hiza MM, Dionne MS, Pham LN, Ayres JS, Schneider DS. Interactions between circadian rhythm and immunity in Drosophila melanlogaster. Curr Biol. 2007;17:R353–5.
Pumpuni CB, Demaio J, Kent M, Davis JR, Beier JC. Bacterial population dynamics in three Anopheline species: the impact on Plasmodium sporogonic development. Am J Trop Med Hyg. 1996;54:214–8.
Uchida K. Balanced amino acid composition essential for infusion-induced egg development in the mosquito (Culex pipiens pallens). J Insect Physiol. 1993;39:615–21.
Jain SK, Hochstein P. Generation of superoxide radicals by hydrazine: its role in phenylhydrazine-induced hemolytic anemia. Biochim Biophys Acta Gen Subj. 1979;586:128–36.
Hurd H. Manipulation of medically important insect vectors by their parasites. Annu Rev Entomol. 2003;48:141–61.
Hogg JC, Hurd H. Malaria induced reduction of fecundity during the first gonotrophic cycle of Anopheles stephensi mosquitoes. Med Vet Entomol. 1995;9:176–80.
Hogg JC, Hurd H. The effects of natural Plasmodium falciparum infection on the fecundity and mortality of Anopheles gambiae sl in north east Tanzania. Parasitology. 1997;114:325–31.
Vezilier J, Nicot A, Gandon S, Rivero A. Plasmodium infection brings forward mosquito oviposition. Biol Lett. 2015;11:20140840.
Pigeault R, Caudron Q, Nicot A, Rivero A, Gandon S. Timing malaria transmission with mosquito fluctuations. Evol Lett. 2018;2–4:378–89.
Ferguson HM, Read AF. Why is the effect of malaria parasites on mosquito survival still unresolved? Trends Parasitol. 2002;18:256–61.
Minchella DJ, Loverde PT. A cost of increased early reproductive effort in the snail Biomphalaria glabrata. Am Nat. 1981;118:876–81.
Agnew P, Koella JC, Michalakis Y. Host life history responses to parasitism. Microbes Infect. 2000;2:891–6.
Garza-Hernández JA, Rodríguez-Pérez MA, Salazar MI, Russell TL, Adeleke MA, de Luna-Santillana EdJ, et al. Vectorial capacity of Aedes aegypti for dengue virus type 2 is reduced with co-infection of Metarhizium anisopliae. PLoS Negl Trop Dis. 2013;7:e2013.
Almeida F, Moura AS, Cardoso AF, Winter CE, Bijovsky AT, Suesdek L. Effects of Wolbachia on fitness of Culex quinquefasciatus (Diptera; Culicidae). Infect Genet Evol. 2011;11:2138–43.
Rono MK, Whitten MM, Oulad-Abdelghani M, Levashina EA, Marois E. The major yolk protein vitellogenin interferes with the anti-plasmodium response in the malaria mosquito Anopheles gambiae. PLoS Biol. 2010;8:e1000434.
Costa G, Gildenhard M, Eldering M, Lindquist RL, Hauser AE, Sauerwein R, et al. Non-competitive resource exploitation within-mosquito shapes evolution of malaria virulence. bioRxiv. 2017.
Ahmed AM, Baggott SL, Maingon R, Hurd H. The costs of mounting an immune response are reflected in the reproductive fitness of the mosquito Anopheles gambiae. Oikos. 2002;97:371–7.
We thank Aidan McHall-Hagan, Iona Brian, Caroline Rodger and G. K. P. Barra for assistance, and Jess Metcalf for help with statistical analysis.
The Royal Society (202769/Z/16/Z; 204511/Z/16/Z), the Human Frontier Science Programme (RGP0046/2013), and the Wellcome (202769/Z/16/A) funded the work.
Ethics approval and consent to participate
All procedures were carried out in accordance with the UK Animals (Scientific Procedures) Act 1986 (PPL 70/8546).
Consent for publication
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
O’Donnell, A.J., Rund, S.S.C. & Reece, S.E. Time-of-day of blood-feeding: effects on mosquito life history and malaria transmission. Parasites Vectors 12, 301 (2019) doi:10.1186/s13071-019-3513-9
- Biological rhythm
- Circadian rhythm
- Plasmodium berghei
- Plasmodium chabaudi
- Anopheles stephensi