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Further evaluation and validation of the VETSCAN IMAGYST: in-clinic feline and canine fecal parasite detection system integrated with a deep learning algorithm

Parasites & Vectors volume 14, Article number: 89 (2021) Cite this article

Abstract

Background

Fecal examinations in pet cats and dogs are key components of routine veterinary practice; however, their accuracy is influenced by diagnostic methodologies and the experience level of personnel performing the tests. The VETSCAN IMAGYST system was developed to provide simpler and easier fecal examinations which are less influenced by examiners’ skills. This system consists of three components: a sample preparation device, an automated microscope scanner, and analysis software. The objectives of this study were to qualitatively evaluate the performance of the VETSCAN IMAGYST system on feline parasites (Ancylostoma and Toxocara cati) and protozoan parasites (Cystoisospora and Giardia) and to assess and compare the performance of the VETSCAN IMAGYST centrifugal flotation method to reference centrifugal and passive flotation methods.

Methods

To evaluate the diagnostic performance of the scanning and algorithmic components of the VETSCAN IMAGYST system, fecal slides were prepared by the VETSCAN IMAGYST centrifugal flotation technique with pre-screened fecal samples collected from dogs and cats and examined by both an algorithm and parasitologists. To assess the performance of the VETSCAN IMAGYST centrifugal flotation technique, diagnostic sensitivity and specificity were calculated and compared to those of conventional flotation techniques.

Results

The performance of the VETSCAN IMAGYST algorithm closely correlated with evaluations by parasitologists, with sensitivity of 75.8–100% and specificity of 93.1-100% across the targeted parasites. For samples with 50 eggs or less per slide, Lin’s concordance correlation coefficients ranged from 0.70 to 0.95 across the targeted parasites. The results of the VETSCAN IMAGYST centrifugal flotation method correlated well with those of the conventional centrifugal flotation method across the targeted parasites: sensitivity of 65.7–100% and specificity of 97.6–100%. Similar results were observed for the conventional passive flotation method compared to the conventional centrifugal flotation method: sensitivity of 56.4–91.7% and specificity of 99.4–100%.

Conclusions

The VETSCAN IMAGYST scanning and algorithmic systems with the VETSCAN IMAGYST fecal preparation technique demonstrated a similar qualitative performance to the parasitologists’ examinations with conventional fecal flotation techniques. Given the deep learning nature of the VETSCAN IMAGYST system, its performance is expected to improve over time, enabling it to be utilized in veterinary clinics to perform fecal examinations accurately and efficiently.

Graphical Abstract

Background

Domestic cat ownership in the USA has been increasing at the same time as an overall rise in pet ownership. In 2017–2018, up to 47.1 million households in the USA owned at least one cat, with 76% of owners considering their cats to be “family members” [1]. However, cats are generally less medicalized or served by veterinarians compared to dogs [2], even though studies have reported that gastrointestinal parasitism in cats is common [3,4,5,6,7,8]. A recent retrospective study demonstrated a significant increase in the prevalence of intestinal parasitic infections in client-owned cats during a 12-year period (19.0% in 2007 vs. 32.5% in 2018; P < 0.0001) [7]. The majority of people acquire cats from shelters or as rescues and through adoption of strays [1], although these cat populations harbor a high prevalence of parasitic infections, ranging between 31.8 and 67.2% [3,4,5, 7, 8]. The most prevalent nematodes identified in cats are Ancylostoma and Toxocara cati [3,4,5,6,7,8,9]. The main species of feline Ancylostoma found in North America are Ancylostoma tubaeforme and Ancylostoma braziliense [10]. Since feline Ancylostoma and T. cati are zoonotic parasites [11,12,13,14,15,16,17,18], it is important to conduct routine fecal examinations of cats, and treat them as necessary to maintain their wellness and that of their owners.

The most commonly detected protozoan parasites in domestic cats and dogs in North America are Cystoisospora (formally Isospora) and Giardia [3, 5,6,7,8, 19,20,21,22,23,24,25]. Studies evaluating feline fecal samples have reported the prevalence of Cystoisospora and Giardia to range between 3.8–26.0% and 1.2–9.9%, respectively, with the highest prevalence in shelter cats [3, 5,6,7,8, 19, 20, 24, 25]. The prevalence of Cystoisospora and Giardia in dogs has been reported to range between 0.5–10.4% and 3.3–13%, respectively [8, 19, 21,22,23,24, 26,27,28,29,30,31]. Cystoisospora oocysts and Giardia cysts/trophozoites are small in size and can be challenging to detect by microscopy, especially when fecal samples contain a low number of oocysts, cysts, or trophozoites. In addition, intermittent shedding of Giardia cysts/trophozoites makes it more difficult to diagnose giardiasis [10, 32,33,34,35].

Fecal examinations in dogs and cats to detect evidence of gastrointestinal parasitism are widely recognized as an important component of routine veterinary care, both for maintaining pet health and for identifying parasites of zoonotic significance [36]. However, the accuracy and usefulness of fecal testing can be influenced by many factors, such as differences in methodology and the experience level of personnel conducting the tests [36,37,38]. Of note, Giardia has been identified as a particularly difficult parasite to diagnose by coprology in veterinary practice [36]. Computer-based algorithms to identify parasites in fecal examinations have recently been developed and introduced with the aim of improving the accuracy and consistency of diagnosing parasitic diseases in dogs and cats [39,40,41,42].

The novel VETSCAN IMAGYST system evaluates fecal samples for evidence of parasitic infections in an organized and uncomplicated fashion that does not depend greatly on an examiner’s level of experience. This system was able to reliably detect four targeted parasites (genera Ancylostoma, Toxocara, Trichuris, and family Taeniidae) in fecal samples of 84 dogs and 16 cats, and the results closely correlated with those reported by a parasitologist following fecal examination of the animals (Pearson correlation coefficients 0.83–0.99 across the four targeted parasites) [40]. The VETSCAN IMAGYST system consists of three main components: a sample preparation device, an automated commercially available microscope scanner, and a data analysis process using deep neural networks (Fig. 1). The VETSCAN IMAGYST system applies a deep learning object detection algorithm which uses a convolutional neural network to identify convolutional layers that automatically learn the most discriminating features between classes (Fig. 2). The algorithm assigns a probability score to each image recognized within each sample preparation as being that of an egg belonging to a parasite genus/group that the software has been previously trained/calibrated to recognize. A mature algorithm model, which can perceive and distinguish the morphology of individual parasite eggs and non-parasite objects on fecal flotation slides, is developed through a training process that utilizes samples characterized by an expert. Once the model is sufficiently mature, it is tested against several evaluation datasets to ensure that the final most promising model will be generalizable to other similar domains.

Fig. 1
figure 1

The VETSCAN IMAGYST system consists of three main components: a sample preparation device, a digital microscope scanner, and data analysis and reporting

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Fig. 2
figure 2

Diagram of the VETSCAN IMAGYST algorithm’s convolution neural network and object detection process

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The present study is an extension of a previously published VETSCAN IMAGYST study [40], with two additional main objectives. First, to qualitatively evaluate the diagnostic performance of the VETSCAN IMAGYST system to recover and correctly identify feline Ancylostoma eggs, Toxocara cati eggs, Cystoisospora oocysts, and Giardia cysts in feces of naturally infected cats and dogs, compared to manual identification by experts with conventional sample preparation methods. Second, to compare the diagnostic performance of the VETSCAN IMAGYST centrifugal preparation method to those of standard centrifugal and passive flotation methods for the four targeted parasites: feline Ancylostoma, Toxocara cati, Cystoisospora, and Giardia.

Methods

Fecal sample collections

Fecal samples from client-owned and shelter cats and dogs submitted to the Oklahoma Animal Disease Diagnostic Laboratory of Oklahoma State University were processed for egg, oocyst, and cyst recovery using a Wisconsin fecal egg counting method, as previously described [10, 40]. A total of 100 fecal samples were collected weighing a minimum of 8 g and confirmed positive for at least one of the targeted parasites: (1) feline Ancylostoma, (2) Toxocara cati, (3) feline Cystoisospora, and (4) canine and feline Giardia; as well as 10 feline samples negative for any targeted parasites. Samples were included as positives or negatives for the relevant analyses; samples containing multiple targeted parasites were counted as a positive for more than one analysis, and some samples were counted as a negative for more than one analysis. For the Cystoisospora analysis, 100 additional fecal samples from a second collection separated from the first by several months, with the same sample acquisition criteria including canine samples, were included. Table 1 summarizes the number of screened positive and negative samples assessed for each evaluation. Different reference methods were used for the VETSCAN IMAGYST algorithm and sample preparation assessments; therefore, the total number of positives and negatives varies between evaluations. To maintain morphological integrity of the diagnostic forms of parasite elements, all samples were preserved at 4 °C until the study. For 38 samples, a low level of formalin solution was added as a fixative to ensure integrity of the parasite elements.

Table 1 Summary of fecal samples included in this study by targeted parasites based upon initial characterization
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VETSCAN IMAGYST scanner and algorithm

The VETSCAN IMAGYST system has previously been described [39]. Briefly, slides with fecal samples were read by the Motic EasyScan One digital slide scanner (Motic, Kowloon Bay, Hong Kong), which provided 40× effective resolution. The scanned images were then automatically uploaded and analyzed in the cloud with an updated deep learning objective detection algorithm, version 3033, which was developed based on the You only look once (YOLOv3) model [43] using the adaptive moment estimation (Adam; an algorithm for first-order gradient-based optimization of stochastic objective functions) optimizer [44] (Techcyte, Lindon, UT). Version 3033 was selected after several trainings of eight epochs with approximately 2,000–5,500 images that were previously captured by the VETSCAN IMAGYST system and reserved to improve the algorithm performance. Twice during each epoch, a 416 × 416-pixel area was randomly translated around every label to be used for further trainings. After localization and classification of the objects of interest, the resulting images were then available for viewing on the VETSCAN IMAGYST platform, and a downloadable portable document format report was generated (Fig. 1). The VETSCAN IMAGYST analysis software also has the quantitative ability to count the parasites of interest. In the present study, this quantitative capability was partly evaluated in the samples with ≤ 50 parasite elements/g of feces, as our main objective was to qualitatively evaluate the diagnostic performance of the VETSCAN IMAGYST system recovering and correctly identifying targeted parasites.

Assessment of VETSCAN IMAGYST algorithm performance

To qualitatively evaluate the ability of the scanning and algorithmic components of the VETSCAN IMAGYST system to identify diagnostic forms of the targeted parasites, slides were prepared with the VETSCAN IMAGYST centrifugal flotation technique for each pre-screened fecal sample, as described previously by Nagamori et al. [40]. Briefly, pre-screened fecal samples were examined randomly. The VETSCAN IMAGYST fecal preparation device, which was specifically redesigned and produced from Apacor mini Parasep SF (Apacor, Wokingham, UK), was utilized to perform the VETSCAN IMAGYST centrifugal method. The VETSCAN IMAGYST fecal preparation device consists of two tubes: the sample tube with a sample scoop, which can hold approximately 1 g of feces; and the collection tube containing two types of flotation solution, zinc sulfate solution (specific gravity, 1.18) for Giardia samples and sugar solution (specific gravity, 1.25) for the rest of the samples, including Ancylostoma, Toxocara, Cystoisospora, and negatives. Sugar solution was selected for the current analysis as it is more effective than some of the salt solutions used to float dense parasite eggs, is inexpensive, and allows easier maintenance of fecal slides since it does not crystalize quickly [10]. The sample tube and collection tube were firmly screwed together, shaken and centrifuged for 2 min at 300-500 relative centrifugal force (rcf). Following centrifugation, the sample tube was unscrewed from the collection tube. The transfer loop was used to collect diagnostic forms of parasites from the top of the flotation solution and transfer them to a microscope slide (Fig. 3). The coverslip marked with the IMAGYST label was placed such that the IMAGYST label could be read correctly, ensuring that the marked coverslip was accurately placed on the slide. The slide was placed in a slide tray, which was then inserted into an automated microscope scanner, and the resulting digitally scanned image uploaded to a cloud-based server for analysis and result generation.

Fig. 3
figure 3

VETSCAN IMAGYST sample preparation materials

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The VETSCAN IMAGYST fecal preparation was performed by two technicians. After the slides were analyzed by the VETSCAN IMAGYST system, two parasitologists and one technician, all well experienced in diagnostic parasitology, evaluated each slide microscopically at 100×, 200×, and 400× magnification. Identification of the parasites was based on the morphology of eggs, oocysts, and cysts [10]. During the manual examinations by the parasitologists and technician, counts of the entire slide were performed when up to 50 eggs, oocysts, or cysts per each targeted parasite were present. The numbers were estimated where burdens were high (> 50) and, for eggs, were recorded as “medium” for slides with 51-250 eggs per gram (EPG), and “high” for slides with > 250 EPG. The VETSCAN IMAGYST algorithm, on the other hand, provided counts regardless of egg burden. Results from the VETSCAN IMAGYST algorithm and microscopic examinations by experts were compared and statistically analyzed. For Giardia fecal samples, an additional analysis of the subset of samples with > 10 cysts per gram (CPG) was conducted.

Assessment of sample preparation methods

Performance (sensitivity and specificity) of the VETSCAN IMAGYST centrifugal technique was assessed by comparing it to conventional centrifugal flotation and passive flotation methods using visual microscopy. For each fecal sample, slides were prepared by 3 different sample preparation techniques: VETSCAN IMAGYST centrifugal flotation, conventional centrifugal flotation, and conventional passive flotation using the OVASSAY Plus Kit Fecal Flotation Device (Zoetis, Parsippany Troy Hills, NJ). Two technicians prepared the slides for the VETSCAN IMAGYST centrifugal flotation technique as described above. The process for the reference centrifugal flotation technique has previously been described [40]. Briefly, 33% zinc sulfate solution (specific gravity, 1.18) for Giardia samples and Sheather’s sugar solution (specific gravity, 1.25) for the rest of the samples were used to suspend 1 g of feces, which was then strained and placed in a 15-ml centrifuge tube. Flotation solution was added to the tube until a convex meniscus was formed, then a coverslip was added, and the samples centrifuged in a Centra CL2 centrifuge (Thermo Fisher Scientific, Waltham, MA) at approximately 440 rcf for 5 min. After removing the coverslip and placing it on a glass slide, a microscopic examination was performed [10]. The OVASSAY Plus Kit Fecal Flotation Device with 33% zinc sulfate solution (specific gravity, 1.18) was used to perform the passive fecal flotation test, following the manufacturer’s instructions [45]. Several student workers prepared the fecal slides with conventional centrifugal and passive fecal flotation methods, and all the slides were microscopically examined by three experts as described above. The duration of the microscopic examinations by the experts was not restricted or recorded. The diagnostic performance of the VETSCAN IMAGYST centrifugal flotation method was compared with both reference methods, conventional centrifugal and OVASSAY passive flotation.

Statistical analysis

Samples for which any parasite elements were observed, or for which in a separate analysis > 10 CPG for Giardia were recorded, were considered positive. Two by two tables were constructed, and sensitivity and specificity calculated with 95% Jeffreys confidence interval estimates. Lin’s concordance correlation coefficients (ρc) were calculated when ≤ 50 eggs, oocysts or cysts per slide were counted microscopically by the three experts. When > 50 eggs/oocysts/cysts were estimated per slide, the data were not included in the correlation analysis. SAS version 9.4M6 (SAS Institute, Cary, NC) was used for the statistical analysis.

Results

Algorithm performance

Comparisons between the results generated by the VETSCAN IMAGYST system and those recorded by the experts were made to assess the performance of the VETSCAN IMAGYST scanner and algorithm in the identification of eggs, oocysts, and cysts of targeted parasites (Table 2 and Fig. 4). The diagnostic sensitivity and specificity of the VETSCAN IMAGYST system in comparison to the expert assessments ranged from 75.8–100% and 93.1–100%, respectively, for the four targeted parasites. Twelve of 33 (36%) Giardia samples contained a very low number of cysts, i.e. ≤ 10 CPG. The diagnostic sensitivity for Giardia dramatically increased to 95.2% (95% confidence interval: 79.8–99.5%) after the Giardia samples with ≤ 10 CPG were excluded from the analysis. Quantitative comparisons for samples with ≤ 50 EPG, OPG, or CPG were performed, and the VETSCAN IMAGYST diagnostic results closely matched those of the experts for each targeted parasite (ρc of 0.95 for feline Ancylostoma, 0.89 for Toxocara cati, 0.80 for Cystoisospora, and 0.70 for Giardia).

Table 2 Algorithm performance analysis: diagnostic sensitivity and specificity comparing the results reported by an expert (reference) versus by the VETSCAN IMAGYST scanner and algorithm for samples prepared with the VETSCAN IMAGYST centrifugal flotation method
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Fig. 4
figure 4

Algorithm performance analysis: quantitative visualization of egg identification [eggs per gram (EPG)] by an expert parasitologist (x-axis) and the VETSCAN IMAGYST scanner/algorithm (y-axis) for the samples with ≤ 50 EPG prepared by the VETSCAN IMAGYST centrifugal flotation method

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Sample preparation performance

When the performance of the VETSCAN IMAGYST centrifugal flotation (test method) and the conventional centrifugal flotation (reference method) were compared, the diagnostic sensitivity and specificity of the VETSCAN IMAGYST ranged from 65.7–100% and 97.6–100%, respectively, across the four targeted parasites (Table 3). When the sample preparation performance of the OVASSAY passive flotation method was compared to the conventional centrifugation flotation method, the diagnostic sensitivity and specificity of the former ranged from 56.4–91.7% and 99.4–100%, respectively (Table 3).

Table 3 Sample preparation analysis: diagnostic sensitivity and specificity comparing the VETSCAN IMAGYST centrifugal flotation (VS-Ic) and OVASSAY passive flotation (O-pf) methods versus a conventional centrifugal flotation method (reference); all slides were read by an expert
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Discussion

This is the second study demonstrating that the VETSCAN IMAGYST system integrated with a deep learning object detection algorithm can successfully recognize and identify diagnostic forms of gastrointestinal parasites in dogs and cats on fecal flotation slides scanned by an automated microscope. Whereas our previous study evaluated the VETSCAN IMAGYST system for the detection of eggs of Ancylostoma, Toxocara, Trichuris, and Taeniidae in 84 canine and 16 feline fecal samples [40], this study assessed the ability of the system to detect eggs of the feline nematodes Ancylostoma and Toxocara cati, and oocysts of the protozoan parasite Cystoisospora and cysts of the protozoan parasite Giardia, in 104 canine and 96 feline fecal samples, making it a more comprehensive analysis of this novel system.

Although both domestic dogs (Canis lupus familiaris) and cats (Felis catus) belong to the order Carnivora, dogs are classified into the superfamily Canoidea and cats are classified into the superfamily Feloidea [46, 47]. Diets of canids can vary from herbivorous to omnivorous; however, all felids require a strictly carnivorous diet [47]. A high-protein diet of animal origin is essential for domestic cats to obtain some of their nutritional requirements, such as taurine, as well as arachidonic acid and vitamin A [47]. Due to their diet, fecal samples of cats commonly contain a large amount of fat and are soft, sticky and clay-like in consistency, which often makes it more difficult, or sometimes impossible, to read fecal slides since more debris floats with fats, especially when a viscous sugar solution is used in a centrifugal flotation technique. Modification of fecal flotation procedures, involving an initial water wash where the supernatant is discarded after the initial spin and the sediment resuspended with a flotation solution to remove excess fat, mucus, and debris (double centrifugal fecal flotation technique), can be used [10]. In the present study, the VETSCAN IMAGYST centrifugal flotation method recovered parasite elements from feline feces, and the VETSCAN IMAGYST scanner and algorithm successfully captured and identified targeted parasites (Fig. 5).

Fig. 5
figure 5

Images of targeted parasites captured by the VETSCAN IMAGYST system

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To our knowledge, this is the first report demonstrating the ability of the VETSCAN IMAGYST system to recover and accurately detect protozoan parasites, i.e. Cystoisospora oocysts and Giardia cysts. Although coccidiosis is generally considered a self-limiting infection in mature dogs and cats due to their rapid development of immunity [18], Cystoisospora is an ubiquitous and important pathogen in puppies and kittens, with infection often resulting in diarrhea, abdominal pain, anorexia, bloody diarrhea, anemia, and even mortality in severe cases [10, 17, 48]. Since Cystoisospora undergo fast replication in the pathogenic intestinal stage and a high number of oocysts are excreted in host feces, causing environmental contamination, it is considered critical and thus highly recommended to conduct a fecal examination with centrifugation for puppies and kittens at least four times during the first year of life for treatment of Cystoisospora at an early stage of infection [48, 49]. Different species of Cystoisospora are commonly diagnosed in dogs and cats: Cystoisospora canis and Cystoisospora ohioensis in dogs, and Cystoisospora felis and Cystoisospora rivolta in cats. Oocysts of C. canis and C. felis are slightly bigger, at approximately 38-51 × 27-39 µm in size, than those of C. ohioensis and C. rivolta, which are approximately 17-27 x 15-24 µm in size [10]. Due to the smaller size of coccidian oocysts compared to helminth eggs, Cystoisospora can be easily overlooked, especially when the number of oocysts on a fecal slide is low and an inaccurate microscopic focus is used for examination. The VETSCAN IMAGYST system correctly identified oocysts of all four Cystoisospora species in canine and feline fecal samples and successfully reported them as Cystoisospora (coccidia) (Fig. 5).

The diagnostic sensitivity and specificity of the VETSCAN IMAGYST scanner and algorithm for the Giardia samples compared with the results reported by the experts were 75.8 and 97.0%, respectively (Table 2). As previously discussed, a common challenge for many object detection algorithm models is to precisely localize and distinguish small objects such as Giardia cysts [40]. The nature of a deep learning algorithm, however, means that its performance continues to improve with further training. It is important to note that the diagnostic sensitivity was dramatically increased to 95.2% by removing the 12 Giardia samples that had ≤ 10 CPG from the analysis; detecting such a low number of cysts is extremely demanding when slides are examined by visual microscopy. Additionally, the examination and counts of CPG on these Giardia slides were carefully performed by a well-trained diagnostic parasitologist with no time limit, which most likely resulted in a much higher diagnostic performance compared to that usually achieved by technicians in daily veterinary practice.

The detection of Giardia cysts and triphozoites by microscopic examination is generally considered the most sensitive technique for the diagnosis of giardiasis, and therefore, a great deal of training and experience is required for the confident diagnosis of this disease [10]. It is challenging to identify Giardia infection by fecal examination because, in addition to their small size and transparency, Giardia cysts and trophozoites are intermittently shed in feces, and multiple fecal examinations may be necessary to rule out infection. Fresh fecal samples, obtained preferably within 30 min of defecation, are often required to detect motile trophozoites; however, Giardia cysts and trophozoites are fairly fragile and their shape easily distorted in flotation solution [10]. A 33% zinc sulfate solution (specific gravity, 1.18) is preferred and recommended for the detection of Giardia cysts, as other flotation solutions can rapidly cause osmotic damage to them, which increases the difficulty of perceiving them on fecal slides [10, 34, 50, 51]. During the present study, the VETSCAN IMAGYST system effectively recognized and identified both intact and collapsed Giardia cysts (Fig. 5). Testing for Giardia is recommended not only in symptomatic dogs and cats, but also in dogs and cats newly introduced to homes which have other pets that are free of infection, as many Giardia infections can be asymptomatic [10, 32, 48]. Since there is no perfect flotation solution for the recovery of all the different types of parasites [10], it is important to consider the advantages and disadvantages of each individual solution when selecting one for general use. Some experts recommend performing two centrifugal flotation tests by using both Sheather’s sugar and 33% zinc sulfate solutions to achieve a broader range of gastrointestinal parasite detections. In cases where Giardia is suspected, analysis using the sugar flotation solution should also be performed on fecal samples to check for other parasites.

The detection of Giardia is also possible with Giardia-specific coproantigen detection assays [35, 48]. However, when not used in conjunction with a traditional microscopic technique, antigen testing may provide a false positive result in an animal that is no longer infected with Giardia due to persistent antigen excretion for several weeks or even months after parasite elimination [52, 53]. Given the shortcomings of current in-house diagnostic methods for Giardia, utilizing a deep learning algorithm platform, such as the VETSCAN IMAGYST system, could provide clinicians with an excellent additional or alternative diagnostic tool to help identify Giardia cases that would otherwise be missed.

Evaluation of the performance of the VETSCAN IMAGYST centrifugal flotation sample preparation method was limited in this study due to the modest numbers of true positives for the four targeted parasites and the inherent subsampling variability in non-homogenous fecal samples, which has been well documented in previous publications [54]. Kochanowski et al. [54] observed a wide range of coefficients of variation, between 31 and 254%, for Toxocara and Trichuris samples with a low number of egg counts, i.e. ≤ 50 EPG. Despite these limitations, the performance of the VETSCAN IMAGYST centrifugal flotation method in the present study was comparable to a conventional centrifugal flotation method, with diagnostic sensitivity and specificity of the comparisons ranging from 65.7 to 100% and 97.6–100%, respectively, across the four targeted parasites (Table 3). Additionally, one potential modification considered for the VETSCAN IMAGYST centrifugal flotation method to increase its diagnostic sensitivity is to lengthen the duration of centrifugation. Previous studies reported that egg recoveries with centrifugation at 264 × g were significantly improved when the duration of centrifugation was increased from 1 and 3 min to 4 or 5 min at the same speed, although no change was observed in egg recovery when the time of centrifugation was extended to 10 or 20 min [37, 55].

As shown in Table 3, the diagnostic sensitivity and specificity of the VETSCAN IMAGYST centrifugal technique slightly surpassed those of the OVASSAY passive flotation method. Despite the fact that centrifugation significantly increases the sensitivity of fecal examinations, passive flotation continues to be the most commonly used technique in veterinary private practice due to its convenience [10, 19, 36, 51, 56,57,58]. Given that the VETSCAN IMAGYST system reliably recovers and detects parasite elements in fecal samples, does not largely depend on the experience level of examiners, and has previously been shown to provide results in around 10 min with the VETSCAN IMAGYST centrifugal flotation method [40], it has the potential to replace the conventional passive flotation method used in veterinary practice.

The most distinctive and unique feature of the VETSCAN IMAGYST system is its deep learning object detection algorithm. To the best of our knowledge, the VETSCAN IMAGYST system is the only automated diagnostic system that is integrated with a deep learning object detection algorithm and applied to veterinary medicine. Compared to shallow learning systems, which do not have any structural information on the function to be learned, deep learning algorithms exploit the advantage of locality at each level of the layered hierarchy, enabling the system to ignore the aspects that make computer vision brittle [59, 60]. The layered hierarchy also facilitates the system to continuously adapt to new data and apply these to new output classes with fewer examples [60, 61]. The deep learning characteristic, along with the YOLOv3 object detection model [43], which incorporates localization and classification features, results in a decrease of background errors and high agreement between the VETSCAN IMAGYST system and expert examinations. Another benefit of this system is its ability to store images and reports on a secure, cloud-based server system, allowing easy sharing of information by parasitologists as well as members of the veterinary and academic communities for patient care, research, and teaching.

The present study did not evaluate the usability of the VETSCAN IMAGYST system; however, our previous analysis showed that the VETSCAN IMAGYST system with the VETSCAN IMAGYST centrifugal flotation method could produce results in around 10 min, which is comparable to conventional fecal flotation tests. This time frame included the time to prepare the sample, i.e. approximately 3.5 min including the 2-min centrifugal incubation time, and the time to scan the images, i.e. approximately 6–7 min [40]. Data from the present study add to the body of evidence demonstrating the performance of the VETSCAN IMAGYST system in detecting intestinal parasite elements recovered from fecal samples. In addition to identifying the protozoan parasites Cystoisospora and Giardia, results from our present and previous studies show the system’s reliable performance in detecting four different genera/group of gastrointestinal parasites (Ancylostoma, Toxocara, Trichuris, Taeniidae) in dogs and cats [40]. With further training, the VETSCAN IMAGYST system will gain the ability to identify other parasites. The quantitative capability of the VESTSCAN IMAGYST system is currently under development. It is predicted that the algorithm will be able to perform a fecal egg counting test in the future.

Conclusions

The VETSCAN IMAGYST system effectively recovered and identified feline Ancylostoma eggs, Toxocara cati eggs, Cystoisospora oocysts, and Giardia cysts in feline and canine fecal samples. Given the deep learning nature of the VETSCAN IMAGYST system, its performance is expected to improve over time, enabling it to be utilized in veterinary clinics to perform fecal examinations both accurately and efficiently.

Availability of data and materials

All the data generated or analyzed during this study are included in this published article.

References

  1. Animal Sheltering/the Humane Society of the United States, 2020. Pets by the numbers. https://www.animalsheltering.org/page/pets-by-the-numbers. Accessed 15 Apr 2020.

  2. American Veterinary Medical Association (AVMA). Pet ownership stable, veterinary care variable. https://www.avma.org/javma-news/2019-01-15/pet-ownership-stable-veterinary-care-variable Accessed 16 July 2020.

  3. Hoggard KR, Jarriel DM, Bevelock TJ, Verocai GG. Prevalence survey of gastrointestinal and respiratory parasites of shelter cats in northeastern Georgia, USA. Vet Parasitol Reg Stud Rep. 2019;16:100270. https://doi.org/10.1016/j.vprsr.2019.100270.

    Article  Google Scholar 

  4. Little S, Adolph C, Downie K, Snider T, Reichard M. High prevalence of covert infection with gastrointestinal helminths in cats. J Am Anim Hosp Assoc. 2015;51(6):359–64. https://doi.org/10.5326/JAAHA-MS-6221.

    Article  PubMed  Google Scholar 

  5. Lucio-Forster A, Bowman DD. Prevalence of fecal-borne parasites detected by centrifugal flotation in feline samples from two shelters in upstate New York. J Feline Med Surg. 2011;13(4):300–3. https://doi.org/10.1016/j.jfms.2010.12.013.

    Article  PubMed  Google Scholar 

  6. Nagamori Y, Payton ME, Duncan-Decocq R, Johnson EM. Fecal survey of parasites in free-roaming cats in northcentral Oklahoma, United States. Vet Parasitol Reg Stud Rep. 2018;14:50–3. https://doi.org/10.1016/j.vprsr.2018.08.008.

    Article  Google Scholar 

  7. Nagamori Y, Payton ME, Looper E, Apple H, Johnson EM. Retrospective survey of parasitism identified in feces of client-owned cats in North America from 2007 through 2018. Vet Parasitol. 2020;277:109008. https://doi.org/10.1016/j.vetpar.2019.109008.

    Article  CAS  PubMed  Google Scholar 

  8. Villeneuve A, Polley L, Jenkins E, Schurer J, Gilleard J, Kutz S, et al. Parasite prevalence in fecal samples from shelter dogs and cats across the Canadian provinces. Parasites Vectors. 2015;8:281. https://doi.org/10.1186/s13071-015-0870-x.

    Article  PubMed  PubMed Central  Google Scholar 

  9. Loftin CM, Donnett UB, Schneider LG, Varela-Stokes AS. Prevalence of endoparasites in northern Mississippi shelter cats. Vet Parasitol Reg Stud Rep. 2019;18:100322. https://doi.org/10.1016/j.vprsr.2019.100322.

    Article  Google Scholar 

  10. Zajac AM, Conboy GA. Veterinary clinical parasitology. 8th ed. Ames, IA: Blackwell Publishing; 2012.

    Google Scholar 

  11. Companion Animal Parasite Council (CAPC), 2020. CAPC guidelines: hookworms. https://capcvet.org/guidelines/hookworms/. Accessed 21 Apr 2020.

  12. Fisher M. Toxocara cati: an underestimated zoonotic agent. Trends Parasitol. 2003;19(4):167–70. https://doi.org/10.1016/s1471-4922(03)00027-8.

    Article  PubMed  Google Scholar 

  13. Furtado LFV, Dias LTO, Rodrigues TO, Silva VJD, Oliveira V, Rabelo EML. Egg genotyping reveals the possibility of patent Ancylostoma caninum infection in human intestine. Sci Rep. 2020;10(1):3006. https://doi.org/10.1038/s41598-020-59874-8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Lee AC, Schantz PM, Kazacos KR, Montgomery SP, Bowman DD. Epidemiologic and zoonotic aspects of ascarid infections in dogs and cats. Trends Parasitol. 2010;26(4):155–61. https://doi.org/10.1016/j.pt.2010.01.002.

    Article  PubMed  Google Scholar 

  15. Loukas A, Hotez PJ, Diemert D, Yazdanbakhsh M, McCarthy JS, Correa-Oliveira R, et al. Hookworm infection. Nat Rev Dis Primers. 2016;2:16088. https://doi.org/10.1038/nrdp.2016.88.

    Article  PubMed  Google Scholar 

  16. Macpherson CN. The epidemiology and public health importance of toxocariasis: a zoonosis of global importance. Int J Parasitol. 2013;43(12–13):999–1008. https://doi.org/10.1016/j.ijpara.2013.07.004.

    Article  PubMed  Google Scholar 

  17. Taylor MA, Coop RL, Wall RL. Parasites of dogs and cats. In: Taylor MA, Coop RL, Wall RL, editors. Veterinary parasitology. West Sussex: Wiley; 2016. p. 599–677.

    Google Scholar 

  18. Bowman DD. Helminths. In: Bowman DD, editor. Georgis’ parasitology for veterinarians. St. Louis, Missouri: Elsevier Health Sciences; 2014. p. 122–240.

    Google Scholar 

  19. Gates MC, Nolan TJ. Endoparasite prevalence and recurrence across different age groups of dogs and cats. Vet Parasitol. 2009;166(1–2):153–8. https://doi.org/10.1016/j.vetpar.2009.07.041.

    Article  PubMed  PubMed Central  Google Scholar 

  20. Hoopes JH, Polley L, Wagner B, Jenkins EJ. A retrospective investigation of feline gastrointestinal parasites in western Canada. Can Vet J. 2013;54(4):359–62.

    PubMed  PubMed Central  Google Scholar 

  21. Jacobs SR, Forrester CP, Yang J. A survey of the prevalence of Giardia in dogs presented to Canadian veterinary practices. Can Vet J. 2001;42(1):45–6.

    CAS  PubMed  PubMed Central  Google Scholar 

  22. Joffe D, Van Niekerk D, Gagne F, Gilleard J, Kutz S, Lobingier R. The prevalence of intestinal parasites in dogs and cats in Calgary Alberta. Can Vet J. 2011;52(12):1323–8.

    PubMed  PubMed Central  Google Scholar 

  23. Nagamori Y, Payton ME, Looper E, Apple H, Johnson EM. Retrospective survey of endoparasitism identified in feces of client-owned dogs in North America from 2007 through 2018. Vet Parasitol. 2020;282:109137. https://doi.org/10.1016/j.vetpar.2020.109137.

    Article  CAS  PubMed  Google Scholar 

  24. Olson ME, Leonard NJ, Strout J. Prevalence and diagnosis of Giardia infection in dogs and cats using a fecal antigen test and fecal smear. Can Vet J. 2010;51(6):640–2.

    PubMed  PubMed Central  Google Scholar 

  25. Wyrosdick HM, Chapman A, Martinez J, Schaefer JJ. Parasite prevalence survey in shelter cats in Citrus County, Florida. Vet Parasitol Reg Stud Rep. 2017;10:20–4. https://doi.org/10.1016/j.vprsr.2017.07.002.

    Article  Google Scholar 

  26. Companion Animal Parasite Council (CAPC), 2020. Giardia prevalence. https://capcvet.org/maps/#2020/all/giardia/dog/united-states/ Accessed 19 July 2020.

  27. Blagburn BL, Lindsay DS, Vaughan JL, Rippey NS, Wright JC, Lynn RC, et al. Prevalence of canine parasites based on fecal flotation. The compendium on continuing education for the practicing veterinarian (USA). 1996. https://www.researchgate.net/publication/279867899_Prevalence_of_canine_parasites_based_on_fecal_flotation.

  28. Jordan HE, Mullins ST, Stebbins ME. Endoparasitism in dogs: 21,583 cases (1981–1990). J Am Vet Med Assoc. 1993;203(4):547–9.

    CAS  PubMed  Google Scholar 

  29. Little SE, Johnson EM, Lewis D, Jaklitsch RP, Payton ME, Blagburn BL, et al. Prevalence of intestinal parasites in pet dogs in the United States. Vet Parasitol. 2009;166(1–2):144–52. https://doi.org/10.1016/j.vetpar.2009.07.044.

    Article  PubMed  Google Scholar 

  30. Nolan TJ, Smith G. Time series analysis of the prevalence of endoparasitic infections in cats and dogs presented to a veterinary teaching hospital. Vet Parasitol. 1995;59(2):87–96. https://doi.org/10.1016/0304-4017(94)00742-u.

    Article  CAS  PubMed  Google Scholar 

  31. Stafford K, Kollasch TM, Duncan KT, Horr S, Goddu T, Heinz-Loomer C, et al. Detection of gastrointestinal parasitism at recreational canine sites in the USA: the DOGPARCS study. Parasites Vectors. 2020;13(1):275. https://doi.org/10.1186/s13071-020-04147-6.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Companion Animal Parasite Council (CAPC), 2020. CAPC Guidelines: Giardia. https://capcvet.org/guidelines/giardia/. Accessed 20 Apr 2020.

  33. Little SE, Lindsay DS. Laboratory diagnosis of protozoal Infections. In: Sykes JE, Greene CE, editors. Infectious diseases of the dog and cat. 4th ed. St. Louis, Missouri: Elsevier; 2012. p. 711–5.

    Google Scholar 

  34. Kirkpatrick CE. Feline giardiasis: a review. J Small Anim Pract. 1986;27(2):69–80. https://doi.org/10.1111/j.1748-5827.1986.tb02124.x.

    Article  Google Scholar 

  35. McHardy IH, Wu M, Shimizu-Cohen R, Couturier MR, Humphries RM. Detection of intestinal protozoa in the clinical laboratory. J Clin Microbiol. 2014;52(3):712–20. https://doi.org/10.1128/JCM.02877-13.

    Article  PubMed  PubMed Central  Google Scholar 

  36. Dryden MW, Payne PA, Ridley RK, Smith VE. Gastrointestinal parasites: the practice guide to accurate diagnosis and treatment. Suppl Compend Contin Educ Vet. 2006;28:8.

    Google Scholar 

  37. Ballweber LR, Beugnet F, Marchiondo AA, Payne PA. American Association of Veterinary Parasitologists’ review of veterinary fecal flotation methods and factors influencing their accuracy and use—is there really one best technique? Vet Parasitol. 2014;204(1–2):73–80. https://doi.org/10.1016/j.vetpar.2014.05.009.

    Article  CAS  PubMed  Google Scholar 

  38. Gates MC, Nolan TJ. Comparison of passive fecal flotation run by veterinary students to zinc-sulfate centrifugation flotation run in a diagnostic parasitology laboratory. J Parasitol. 2009;95(5):1213–4. https://doi.org/10.1645/GE-2058.1.

    Article  PubMed  Google Scholar 

  39. Inacio SV, Ferreira Gomes J, Xavier Falcao A, Nagase Suzuki CT, Bertequini Nagata W, Nery Loiola SH, et al. Automated diagnosis of canine gastrointestinal parasites using image analysis. Pathogens. 2020;9:2. https://doi.org/10.3390/pathogens9020139.

    Article  Google Scholar 

  40. Nagamori Y, Hall Sedlak R, DeRosa A, Pullins A, Cree T, Loenser M, et al. Evaluation of the VETSCAN IMAGYST: an in-clinic canine and feline fecal parasite detection system integrated with a deep learning algorithm. Parasites Vectors. 2020;13(1):346. https://doi.org/10.1186/s13071-020-04215-x.

    Article  PubMed  PubMed Central  Google Scholar 

  41. Scare JA, Slusarewicz P, Noel ML, Wielgus KM, Nielsen MK. Evaluation of accuracy and precision of a smartphone based automated parasite egg counting system in comparison to the McMaster and Mini-FLOTAC methods. Vet Parasitol. 2017;247:85–92. https://doi.org/10.1016/j.vetpar.2017.10.005.

    Article  CAS  PubMed  Google Scholar 

  42. Slusarewicz P, Pagano S, Mills C, Popa G, Chow KM, Mendenhall M, et al. Automated parasite faecal egg counting using fluorescence labelling, smartphone image capture and computational image analysis. Int J Parasitol. 2016;46(8):485–93. https://doi.org/10.1016/j.ijpara.2016.02.004.

    Article  PubMed  Google Scholar 

  43. Redmon J, Farhadi A. YOLOv3: an incremental improvement. arXiv preprint. 2018;1804.02767. https://pjreddie.com/media/files/papers/YOLOv3.pdf.

  44. Kingma DP, Ba JL. ADAM: a method for stochastic optimazation. arXiv preprint. 2017;1412.6980. https://arxiv.org/pdf/1412.6980.pdf.

  45. Zoetis, OVASSAY® plus kit fecal flotation device, https://www.zoetisus.com/products/diagnostics/OVASSAY®-plus-kit-fecal-flotation-devices.aspx.

  46. MacDonald ML, Rogers QR, Morris JG. Nutrition of the domestic cat, a mammalian carnivore. Annu Rev Nutr. 1984;4:521–62. https://doi.org/10.1146/annurev.nu.04.070184.002513.

    Article  CAS  PubMed  Google Scholar 

  47. Salaun F, Blanchard G, Le Paih L, Roberti F, Niceron C. Impact of macronutrient composition and palatability in wet diets on food selection in cats. J Anim Physiol Anim Nutr (Berl). 2017;101(2):320–8. https://doi.org/10.1111/jpn.12542.

    Article  CAS  Google Scholar 

  48. European Scientific Counsel Companion Animal Parasites (ESCCAP), 2018. Control of intestinal protozoa in dogs and cats. https://www.esccap.org/uploads/docs/xnqpgri2_0701_ESCCAP_Guideline_GL6_v7_1p.pdf. Accessed 20 Apr 2020.

  49. Companion Animal Parasite Council (CAPC), 2020. General guidelines. https://capcvet.org/guidelines/general-guidelines/ Accessed 27 July 2020.

  50. Kirkpatrick CE. Giardiasis. Vet Clin North Am Small Anim Pract. 1987;17(6):1377–87. https://doi.org/10.1016/s0195-5616(87)50007-9.

    Article  CAS  PubMed  Google Scholar 

  51. Zajac AM, Johnson J, King SE. Evaluation of the importance of centrifugation as a component of zinc sulfate fecal flotation examinations. J Am Anim Hosp Assoc. 2002;38(3):221–4. https://doi.org/10.5326/0380221.

    Article  PubMed  Google Scholar 

  52. Garcia LS, Arrowood M, Kokoskin E, Paltridge GP, Pillai DR, Procop GW, et al. Laboratory diagnosis of parasites from the gastrointestinal tract. Clin Microbiol Rev. 2018. https://doi.org/10.1128/CMR.00025-17.

    Article  PubMed  Google Scholar 

  53. Rishniw M, Liotta J, Bellosa M, Bowman D, Simpson KW. Comparison of 4 Giardia diagnostic tests in diagnosis of naturally acquired canine chronic subclinical giardiasis. J Vet Intern Med. 2010;24(2):293–7. https://doi.org/10.1111/j.1939-1676.2010.0475.x.

    Article  CAS  PubMed  Google Scholar 

  54. Kochanowski M, Dabrowska J, Karamon J, Cencek T, Osinski Z. Analysis of the accuracy and precision of the McMaster method in detection of the eggs of Toxocara and Trichuris species (Nematoda) in dog faeces. Folia Parasitol (Praha). 2013;60(3):264–72. https://doi.org/10.14411/fp.2013.030.

    Article  PubMed  Google Scholar 

  55. Egwang TG, Slocombe JO. Evaluation of the Cornell-Wisconsin centrifugal flotation technique for recovering trichostrongylid eggs from bovine feces. Can J Comp Med. 1982;46(2):133–7.

    CAS  PubMed  PubMed Central  Google Scholar 

  56. Broussard JD. Optimal fecal assessment. Clin Tech Small Anim Pract. 2003;18(4):218–30. https://doi.org/10.1016/S1096-2867(03)00076-8.

    Article  PubMed  Google Scholar 

  57. Dryden MW, Payne PA, Ridley R, Smith V. Comparison of common fecal flotation techniques for the recovery of parasite eggs and oocysts. Vet Ther. 2005;6(1):15–28.

    CAS  PubMed  Google Scholar 

  58. O’Grady MR, Slocombe JO. An investigation of variables in a fecal flotation technique. Can J Comp Med. 1980;44(2):148–57.

    CAS  PubMed  PubMed Central  Google Scholar 

  59. Krizhevsky A, Sutskever I, Hinton G. ImageNet classification with deep convolutional neural networks. In NeurIPS. 2012. https://papers.nips.cc/paper/4824-imagenet-classification-with-deep-convolutional-neural-networks.pdf

  60. Poggio T, Mhaskar H, Rosasco L, Miranda B, Liao Q. Why and when can deep-but not shallow-networks avoid the curse of dimensionality: a review. Int J Autom Comput. 2017;14(5):503–19. https://doi.org/10.1007/s11633-017-1054-2.

    Article  Google Scholar 

  61. Yosinski J, Clune J, Bengio Y, Lipson H. How transferable are features in deep neural networks? In: Advances in sneural information processing systems. 2014. https://arxiv.org/pdf/1411.1792.pdf.

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Acknowledgements

The authors thank Dr. Ruth Scimeca and Emily Looper for helping to prepare the fecal samples and read the flotation slides. The authors also thank all the personnel who were involved in collecting the fecal samples from dogs and cats for this study. Litto Communications, LLC assisted in preparing the manuscript.

Funding

The studies reported here were funded by Zoetis.

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Authors and Affiliations

  1. Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK, 74078, USA

    Yoko Nagamori

  2. Veterinary Medicine Research and Development, Zoetis, 333 Portage Street, Kalamazoo, MI, 49007, USA

    Ruth Hall Sedlak, Andrew DeRosa, Aleah Pullins & Travis Cree

  3. Global Diagnostics, Zoetis, 10 Sylvan Way, Parsippany, NJ, 07054, USA

    Michael Loenser, Cory Penn & Richard Goldstein

  4. Petcare, Zoetis, 10 Sylvan Way, Parsippany, NJ, 07054, USA

    Yoko Nagamori

  5. Techcyte, Incorporated, 384 S 400 W #125, Lindon, UT, 84042, USA

    Benjamin S. Larson & Richard Boyd Smith

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Contributions

YN performed the fecal egg analysis and was a major contributor to the writing of the manuscript. RHS, AD, AP, ML, CP, RG developed the experimental protocols, analyzed the data, and contributed to the writing of the manuscript. TC performed the data summary and analysis. BL and RBS contributed to the technical execution of the study and the manuscript review. All the authors read and approved the final manuscript.

Corresponding author

Correspondence to Yoko Nagamori.

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Competing interests

RHS, AD, AP, ML, CP, RG, and TC are currently employees of Zoetis. BL and RBS are currently employees of Techcyte. YN was an employee of Oklahoma State University at the time that this study was conducted, and is currently an employee of Zoetis.

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Nagamori, Y., Sedlak, R.H., DeRosa, A. et al. Further evaluation and validation of the VETSCAN IMAGYST: in-clinic feline and canine fecal parasite detection system integrated with a deep learning algorithm. Parasites Vectors 14, 89 (2021). https://doi.org/10.1186/s13071-021-04591-y

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Parasites & Vectors

ISSN: 1756-3305

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