North American import? Charting the origins of an enigmatic Trypanosoma cruzi domestic genotype
Parasites & Vectors volume 5, Article number: 226 (2012)
Trypanosoma cruzi, the agent of Chagas disease, is currently recognized as a complex of six lineages or Discrete Typing Units (DTU): TcI-TcVI. Recent studies have identified a divergent group within TcI - TcIDOM. TcIDOM. is associated with a significant proportion of human TcI infections in South America, largely absent from local wild mammals and vectors, yet closely related to sylvatic strains in North/Central America. Our aim was to examine hypotheses describing the origin of the TcIDOM genotype. We propose two possible scenarios: an emergence of TcIDOM in northern South America as a sister group of North American strain progenitors and dispersal among domestic transmission cycles, or an origin in North America, prior to dispersal back into South American domestic cycles. To provide further insight we undertook high resolution nuclear and mitochondrial genotyping of multiple Central American strains (from areas of México and Guatemala) and included them in an analysis with other published data.
Mitochondrial sequence and nuclear microsatellite data revealed a cline in genetic diversity across isolates grouped into three populations: South America, North/Central America and TcIDOM. As such, greatest diversity was observed in South America (Ar = 4.851, π = 0.00712) and lowest in TcIDOM (Ar = 1.813, π = 0.00071). Nuclear genetic clustering (genetic distance based) analyses suggest that TcIDOM is nested within the North/Central American clade.
Declining genetic diversity across the populations, and corresponding hierarchical clustering suggest that emergence of this important human genotype most likely occurred in North/Central America before moving southwards. These data are consistent with early patterns of human dispersal into South America.
Trypanosoma cruzi, the aetiological agent of Chagas disease, infects 6-8 million people in Latin America, while some 25 million more are at risk of acquiring the disease. Parasite transmission to mammal hosts, including humans, can occur through contact with the faeces of hematophagous triatomine bugs. However, non-vectorial routes are also recognized, including blood transfusion, organ transplantation, congenital transmission, and oral transmission via ingestion of meals contaminated with infected triatomine feces[2, 3].
T. cruzi (family Trypanosomatidae; Euglenozoa: Kinetoplastida) is most closely related to several widely dispersed species of bat trypanosomes. Salivarian trypanosomes including medically important Trypanosoma brucei subspecies, represent a more divergent group. The age of the split between the T. cruzi-containing and T. brucei-containing trypanosome lineages is thought to have been concurrent with the separation of Africa and South America/Antarctica/Australasia 100MYA, implying that T. cruzi and the other Schizotrypanum species evolved exclusively in South America. Others propose an alternative origin of T. cruzi from an ancestral bat trypanosome potentially capable of long range dispersal. Whilst the precise scenario for the arrival of ancestral Schizotrypanum lineages in South America is a matter for debate, the current continental distribution and genetic diversity of T. cruzi supports an origin within South America. Parasite transmission is maintained via hundreds of mammal and triatomine species in different biomes throughout South and Central America, as well as the southern states of the USA.
Biochemical and molecular markers support the existence of six lineages or Discrete Typing Units (DTU): TcI, - TcVI agreed by international consensus (. Each DTU can be loosely associated with a particular ecological and/or geographical framework. TcI is ubiquitous among arboreal sylvatic foci throughout the geographic distribution of T. cruzi and is the major agent of human Chagas disease in northern South America. Several molecular tools now identify substantial genetic diversity within TcI[11–14]. Importantly these new approaches consistently reveal the presence of a genetically divergent and homogeneous TcI group (henceforth TcIDOM – previously TcIa/VENDOM) associated with human infections from Venezuela to Northern Argentina, and largely absent from wild mammals and vectors sampled to date. The origin of this clade is unclear, although recent work supports a sister group relationship with TcI circulating in North America (e.g.[12, 13]).
In this manuscript we have set out to evaluate the genetic diversity of TcI in North/Central America, undertaking a comparison with TcI diversity in South America, including TcIDOM. Our aim was to examine hypotheses describing the origin of the TcIDOM clade. We propose two possible scenarios: an emergence of TcIDOM in northern South America as a sister group of North American strains and dispersal among domestic transmission cycles, or an origin in North America, prior to dispersal back into South American domestic cycles, possibly anthropically. To provide further insight into this question we undertook high resolution nuclear and mitochondrial genotyping of multiple Central American strains (from areas of México and Guatemala) and included them in an analysis with other published data[11–13].
A panel of 72 TcI isolates and clones was assembled for analysis (Table1)[11–16]. Of these, existing sequences and microsatellite data were available for 46 isolates[11, 12]. Isolates were classified into three populations: TcINORTH-CENT, TcISOUTH and TcIDOM. TcINORTH-CENT includes samples from the USA, México, Guatemala and Honduras; TcISOUTH corresponds to South America (Argentina, Bolivia, Colombia, Venezuela and Brazil) and TcIDOM with exclusively domestic isolates from Colombia and Venezuela, already known to correspond to a genotype with restricted genetic diversity: TcIa, as previously described by Herrera et al., (2007) and VENDom, as described by Llewellyn et al., (2009). Additional DTU isolates (TcIII-TcIV) were included as out-groups in the mitochondrial analysis.
Isolates from México and Guatemala were characterized to DTU level via the amplification and sequencing of glucose-6-phosphate isomerase (GPI) as previously described by Lauthier et al., (2012). Subsequently, nine maxicircle gene fragments were amplified, sequenced and concatenated from the Méxican and Guatemalan strains according to Messenger et al., 2012 (excluding ND4). Phylogenetic analysis was also conducted as in Messenger et al., 2012. Nineteen nuclear microsatellite loci previously described by Llewellyn et al., 2009, were selected based on their level of TcI intra-lineage resolution. Microsatellite loci were amplified across 21 unpublished biological stocks from México and Guatemala. Reaction conditions were as described previously. Dendrograms based on multilocus allele profiles were constructed also according to Llewellyn et al., 2009.
Maxicircle nucleotide diversity (π) was calculated for TcINORTH-CENT, TcISOUTH and TcIDOM respectively in DNAsp v5. Nuclear allelic diversity was calculated for the same populations using allelic richness (Ar) in FSTAT. The resulting values are shown in Figure1.
Nucleotide sequences per gene fragment are available from GenBank under the accession numbers MURF1 (fragment a): JX431060 - JX431084; MURF1 (fragment b): JX431085 - JX431109; ND1: JX431110 - JX431134; ND5 (fragment a): JX431135 - JX431159; ND5 (fragment b): JX431160 - JX431184; 9S rRNA: JX431185 - JX431209; 12S rRNA: JX431210 - JX431234; COII: JX431235 - JX431259 and CYT b: JX431260 - JX431284.
Across the 3,449 bp final concatenated alignment (including outgroups), a total of 374 variable sites were found. The mitochondrial phylogeny supported the presence of significant diversity among the isolates examined (Figure2). TcIDOM strains formed a monophyletic clade [60% ML BS/0.98 BPP]. The principal division in the phylogeny was between TcISOUTH and TcIDOM/TcINORTH-CENT (98% ML BS/0.98 BPP). However, this division is incomplete, such that a subset of South American strains is also grouped with TcIDOM and TcINORTH-CENT. Thus, it is not possible to conclude that TcIDOM maxicircle sequences nest uniquely among those from TcINORTH-CENT strains. Conversely, a basal relationship of the TcINORTH-CENT to TcIDOM is suggested at the level of nucleotide diversity by population (Figure1), whereby TcIDOM<TcINORTH-CENT<TcISOUTH. Low standard errors about the mean in all three populations, but especially in TcIDOM and TcINORTH-CENT, suggest that sample size had little impact on the accuracy of estimation between populations.
Distance-based clustering using the microsatellite dataset indicated the presence of several well defined clades (Figure3). Importantly in this case the monophyly of North-Central American isolates was corroborated, and TcIDOM clustered firmly within them (bootstrap 65%). By contrast, South American isolates fall into a divergent but diverse clade. Thus the nuclear data provide stronger support for divergence of TcIDOM from within TcINORTH-CENT than the maxicircle phylogeny. Sample size-corrected allelic richness estimates are consistent with hierarchical patterns of clustering based on pair-wise genetic distances. As with the maxicircle dataset, there is a pronounced cline in diversity across the populations studied - Ar TcIDOM< Ar TcINORTH-CENT< Ar TcISouth (Figure1).
TcI dispersion into Central and North America
Using a 100 MYA biogeographic calibration point, molecular clock analyses point to the origin of T. cruzi (sensu stricto) 5 – 1 MYA[21–23] and a most recent common ancestor for TcI at 1.3-0.2 MYA. Reduced genetic diversity among North-Central American isolates by comparison to their southern counterparts is powerful evidence in support of others who suggest that TcI originated in South America[13, 24]. The emergence of TcI in the South occurred prior to either migration across the Isthmus of Panama alongside didelphid marsupials during the Great American Interchange, or perhaps prior to northerly dispersal via volant mammals (e.g. bats).
Origin of TcIDOM
Recent findings indicate a close resemblance between TcIDOM isolates from the northern region of South America and parasite populations from Central and North America by the use of nuclear and mitochondrial markers[11–13]. Indeed SL-IR genotyping suggests a distribution for TcIDOM that now extends as far south as the Argentine Chaco, where multiple sequences have been identified from human and domestic vector sources. Llewellyn et al., (2009) originally hypothesised that a distinct human/domestic clade could be maintained despite the presence of nearby infective sylvatic strains due to the low parasite transmission efficiency by the vector. In this case multiple feeds by domestic vector nymphs are required to infect individuals, as such human – human transmission is far more common than reservoir host - human transmission. Originally this hypothesis was developed to explain the epidemiology of Chagas disease in Venezuela. However, TcIDOM is clearly widespread and recent data propose a date for its emergence 23,000 ± 12,000 years ago. This corresponds to the earliest human colonisation of the Americas. Thus it seems that TcIDOM may be as ancient as humans in South America. Crucially, our data, which show that TcIDOM is nested among North and Central American strains, suggest that this widespread domestic T. cruzi genotype may actually have made first contact with man in North–Central America.
The expansion of limited diversity genotypes into domestic transmission cycles is a familiar story in T. cruzi. This phenomenon seems to have occurred almost simultaneously with TcIDOM (<60,000 YA) in the Southern Cone region but involving DTUs TcV and TcVI. Nonetheless, static human population densities sufficient to support a sustained domestic cycle are presumably vital. For TcIDOM, patterns of genetic diversity suggest early colonizing Amerindians may have been responsible for its southerly migration and dispersal from North/Central America. However, such early settler populations were probably small, dynamic, and inherently unsuitable to sustain transmission of such a genotype. Many questions, therefore, remain unanswered regarding its emergence. Insight could perhaps be drawn from a better understanding of the current distribution and diversity of TcIDOM (including samples from the Southern Cone), patterns of vector population migrations, and even from analysis of ancient DNA (e.g.). We hope this report serves to galvanize efforts towards this understanding, especially among researchers in Central and North America, where many of the answers lie.
Rassi A, Rassi A, Marin-Neto JA: Chagas disease. Lancet. 2010, 375 (9723): 1388-1402. 10.1016/S0140-6736(10)60061-X.
Carlier Y, Torrico F, Sosa-Estnai S, Russomando G, Luquetti A, Frelij H, Albajar Vinas P: Congenital chagas disease: recommendations for diagnosis, treatment and control of newborns, siblings and pregnant women. PLoS Negl Trop Dis. 2011, 5: e1250-10.1371/journal.pntd.0001250.
Alarconde Noya B, Diaz-Bello Z, Colmenares C, Ruiz-Guevara R, Mauriello L, Zavala-Jaspe R, Suarez JA, Abate T, Naranjo L, Paiva M, Rivas L, Castro J, Marques J, Mendoza I, Acquatella H, Torres J, Noya O: Large urban outbreak of orally acquired acute Chagas disease at a school in Caracas, Venezuela. J Infect Dis. 2010, 201 (9): 1308-1315. 10.1086/651608.
Lima L, Silva FM, Neves L, Attias M, Takata CS, Campaner M, de Souza W, Hamilton PB, Teixeira MM: Evolutionary Insights from Bat Trypanosomes: Morphological, Developmental and Phylogenetic Evidence of a New Species, Trypanosoma (Schizotrypanum) erneyi sp. nov., in African Bats Closely Related to Trypanosoma (Schizotrypanum) cruzi and Allied Species. Protist. 2012, 163: 856-872. 10.1016/j.protis.2011.12.003.
Hamilton PB, Stevens JR, Gaunt MW, Gidley J, Gibson WC: Trypanosomes are monophyletic: evidence from genes for glyceraldehyde phosphate dehydrogenase and small subunit ribosomal RNA. Int J Parasitol. 2004, 34 (12): 1393-1404. 10.1016/j.ijpara.2004.08.011.
Stevens JR, Noyes HA, Dover GA, Gibson WC: The ancient and divergent origins of the human pathogenic trypanosomes. Trypanosoma brucei and T. cruzi. Parasitology. 1999, 118 (Pt 1): 107-116.
Hamilton PB, Teixeira MM, Stevens JR: The evolution of Trypanosoma cruzi: the 'bat seeding' hypothesis. Trends Parasitol. 2012, 28 (4): 136-141. 10.1016/j.pt.2012.01.006.
Yeo M, Acosta N, Llewellyn M, Sanchez H, Adamson S, Miles GA, Lopez E, Gonzalez N, Patterson JS, Gaunt MW, de Arias AR, Miles MA: Origins of Chagas disease: Didelphis species are natural hosts of Trypanosoma cruzi I and armadillos hosts of Trypanosoma cruzi II, including hybrids. Int J Parasitol. 2005, 35 (2): 225-233. 10.1016/j.ijpara.2004.10.024.
Zingales B, Andrade SG, Briones MR, Campbell DA, Chiari E, Fernandes O, Guhl F, Lages-Silva E, Macedo AM, Machado CR, Miles MA, Romanha AJ, Sturm NR, Tibayrenc M, Schijman AG: A new consensus for Trypanosoma cruzi intraspecific nomenclature: second revision meeting recommends TcI to TcVI. Mem Inst Oswaldo Cruz. 2009, 104 (7): 1051-1054. 10.1590/S0074-02762009000700021.
Miles MA, Llewellyn MS, Lewis MD, Yeo M, Baleela R, Fitzpatrick S, Gaunt MW, Mauricio IL: The molecular epidemiology and phylogeography of Trypanosoma cruzi and parallel research on Leishmania: looking back and to the future. Parasitology. 2009, 136 (12): 1509-1528. 10.1017/S0031182009990977.
Ramírez J, Guhl F, Messenger L, Lewis M, Montilla M, Cucunuba Z, Miles M, Llewellyn M: Contemporary cryptic sexuality in Trypanosoma cruzi. Mol Ecol. 2012, 21: 4216-26. 10.1111/j.1365-294X.2012.05699.x.
Messenger LA, Llewellyn MS, Bhattacharyya T, Franzen O, Lewis MD, Ramirez JD, Carrasco HJ, Andersson B, Miles MA: Multiple mitochondrial introgression events and heteroplasmy in Trypanosoma cruzi revealed by maxicircle MLST and next generation sequencing. PLoS Negl Trop Dis. 2012, 6 (4): e1584-10.1371/journal.pntd.0001584.
Llewellyn MS, Miles MA, Carrasco HJ, Lewis MD, Yeo M, Vargas J, Torrico F, Diosque P, Valente V, Valente SA, Gaunt MW: Genome-scale multilocus microsatellite typing of Trypanosoma cruzi discrete typing unit I reveals phylogeographic structure and specific genotypes linked to human infection. PLoS Pathog. 2009, 5 (5): e1000410-10.1371/journal.ppat.1000410.
Cura CI, Mejia-Jaramillo AM, Duffy T, Burgos JM, Rodriguero M, Cardinal MV, Kjos S, Gurgel-Goncalves R, Blanchet D, De Pablos LM, Tomasini N, da Silva A, Russomando G, Cuba CA, Aznar C, Abate T, Levin MJ, Osuna A, Gurtler RE, Diosque P: Trypanosoma cruzi I genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes. Int J Parasitol. 2010, 40 (14): 1599-1607. 10.1016/j.ijpara.2010.06.006.
Pennington PM, Paiz C, Grajeda LM, Cordon-Rosales C: Short report: concurrent detection of Trypanosoma cruzi lineages I and II in domestic Triatoma dimidiata from Guatemala. Am J Trop Med Hyg. 2009, 80 (2): 239-241.
Bucio MI, Cabrera M, Segura EL, Zenteno E, Salazar-Schettino M: Identification of immunodominant antigens in Mexican strains of Trypanosoma cruzi. Immunol Invest. 1999, 28 (4): 257-268. 10.3109/08820139909060860.
Herrera C, Bargues MD, Fajardo A, Montilla M, Triana O, Vallejo GA, Guhl F: Identifying four Trypanosoma cruzi I isolate haplotypes from different geographic regions in Colombia. Infect Genet Evol. 2007, 7 (4): 535-539. 10.1016/j.meegid.2006.12.003.
Lauthier JJ, Tomasini N, Barnabe C, Rumi MM, D'Amato AM, Ragone PG, Yeo M, Lewis MD, Llewellyn MS, Basombrio MA, Miles MA, Tibayrenc M, Diosque P: Candidate targets for Multilocus Sequence Typing of Trypanosoma cruzi: validation using parasite stocks from the Chaco Region and a set of reference strains. Infect Genet Evol. 2012, 12 (2): 350-358. 10.1016/j.meegid.2011.12.008.
Librado P, Rozas J: DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 2009, 25 (11): 1451-1452. 10.1093/bioinformatics/btp187.
Goudet J: FSTAT Version 1.2: a computer program to calculate F-statistics. J Heredity. 1995, 86: 485-486.
Flores-Lopez CA, Machado CA: Analyses of 32 loci clarify phylogenetic relationships among Trypanosoma cruzi lineages and support a single hybridization prior to human contact. PLoS Negl Trop Dis. 2011, 5 (8): e1272-10.1371/journal.pntd.0001272.
Lewis MD, Llewellyn MS, Yeo M, Acosta N, Gaunt MW, Miles MA: Recent, Independent and Anthropogenic Origins of Trypanosoma cruzi Hybrids. PLoS Negl Trop Dis. 2011, 5 (10): e1363-10.1371/journal.pntd.0001363.
Machado CA, Ayala FJ: Nucleotide sequences provide evidence of genetic exchange among distantly related lineages of Trypanosoma cruzi. Proc Natl Acad Sci U S A. 2001, 98 (13): 7396-7401. 10.1073/pnas.121187198.
Barnabe C, Yaeger R, Pung O, Tibayrenc M: Trypanosoma cruzi: a considerable phylogenetic divergence indicates that the agent of Chagas disease is indigenous to the native fauna of the United States. Exp Parasitol. 2001, 99 (2): 73-79. 10.1006/expr.2001.4651.
Marshall LG, Sempere T: Evolution of the neotropical Cenozoic land mammal fauna in its geochronologic, stratigraphic, and tectonic context. Biological relationships between Africa and South America. Edited by: Goldblatt P. 1993, Yale University Press, New Haven, 329-392.
Goebel T, Waters MR, O'Rourke DH: The late Pleistocene dispersal of modern humans in the Americas. Science. 2008, 319 (5869): 1497-1502. 10.1126/science.1153569.
Lima VS, Iniguez AM, Otsuki K, Fernando Ferreira L, Araujo A, Vicente AC, Jansen AM: Chagas disease in ancient hunter-gatherer population, Brazil. Emerg Infect Diseases. 2008, 14 (6): 1001-1002. 10.3201/eid1406.0707.
FZE received an MSc scholarship from the Méxican Council of Science and Technology (CONACyT), and financial support from the International Agency of National Public Health Institutes. LAM, MDL, MAM and MSL acknowledge support from the European FP7 Project ChagasEpiNet, Grant 223034. MSL would like to thank Juan David Ramirez and Prof. Felipe Guhl at the Universidad de los Andes, Colombia for constructive discussion.
The authors declare no competing financial interests. The funder played no role in the study design.
FZE wrote the article, performed the experiments and analysed the data. LAM analysed the data and wrote the article. MAM, TLO, PM, MDL contributed reagents and wrote the paper. CFL analysed the data. JMI, HC, MS contributed reagents. MSL conceived the experiments, analysed the data and wrote the article. All authors read and approved the final version of the manuscript.
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Zumaya-Estrada, F.A., Messenger, L.A., Lopez-Ordonez, T. et al. North American import? Charting the origins of an enigmatic Trypanosoma cruzi domestic genotype. Parasites Vectors 5, 226 (2012). https://doi.org/10.1186/1756-3305-5-226