- Open Access
Abscisic acid induces a transient shift in signaling that enhances NF-κB-mediated parasite killing in the midgut of Anopheles stephensi without reducing lifespan or fecundity
Parasites & Vectors volume 10, Article number: 333 (2017)
Abscisic acid (ABA) is naturally present in mammalian blood and circulating levels can be increased by oral supplementation. We showed previously that oral ABA supplementation in a mouse model of Plasmodium yoelii 17XNL infection reduced parasitemia and gametocytemia, spleen and liver pathology, and parasite transmission to the mosquito Anopheles stephensi fed on these mice. Treatment of cultured Plasmodium falciparum with ABA at levels detected in our model had no effects on asexual growth or gametocyte formation in vitro. However, ABA treatment of cultured P. falciparum immediately prior to mosquito feeding significantly reduced oocyst development in A. stephensi via ABA-dependent synthesis of nitric oxide (NO) in the mosquito midgut.
Here we describe the mechanisms of effects of ABA on mosquito physiology, which are dependent on phosphorylation of TGF-β-activated kinase 1 (TAK1) and associated with changes in homeostatic gene expression and activity of kinases that are central to metabolic regulation in the midgut epithelium. Collectively, the timing of these effects suggests a transient physiological shift that enhances NF-κB-dependent innate immunity without significantly altering mosquito lifespan or fecundity.
ABA is a highly conserved regulator of immune and metabolic homeostasis within the malaria vector A. stephensi with potential as a transmission-blocking supplemental treatment.
We showed previously that oral supplementation of mice with the isoprenoid abscisic acid (ABA) increased circulating levels of ABA and reduced parasitemia, gametocytemia, and disease pathology as well as transmission of Plasmodium yoelii 17XNL to Anopheles stephensi . In parallel studies, ABA had no direct effects on growth of cultured P. falciparum asexual stages or gametocyte development in vitro. In A. stephensi, ABA ingestion modestly induced expression of two antimicrobial peptide genes and markedly (13-fold) induced nitric oxide synthase (nos) midgut transcript levels in mosquitoes fed on supplemented P. falciparum culture relative to controls at 4–6 h post-feeding. Addition of the NOS inhibitor L-NAME confirmed that ABA-dependent NO synthesis in the mosquito midgut eliminated parasites prior to oocyst formation . Given that A. stephensi NO synthesis is regulated by multiple signaling pathways with broad effects on mosquito physiology [2,3,4], the timing and mechanism(s) whereby ABA induces NO synthesis could provide significant insights into the potential effects of ABA on other life history traits that are important to vectorial capacity, including lifespan and egg production .
ABA is an ancient signaling molecule, with known biology in plants, invertebrates, and mammals, likely acting through pathways that have been conserved over evolutionary time. In mammalian studies, ABA has been shown to stimulate insulin release from pancreatic beta cells . In A. stephensi, the insulin- and insulin-like growth factor (IIS) pathway can coordinately regulate anti-parasite defenses as well as mosquito lifespan and reproduction [7,8,9,10,11,12,13]. These effects are due, in part, to IIS-dependent changes in midgut intermediary metabolism, mitochondrial function, and epithelial homeostasis [10, 11, 13, 14]. ABA signaling in plants has been associated with activation of mitogen-activated protein kinases (MAPKs), which regulate pathogen sensing and developmental processes as well as senescence [15, 16]. Notably, nearly 25% of the 1500 ABA-regulated genes in plants derive from ABA-dependent activation of a single MAP2K known as MKK3 . ABA signaling also impacts intermediary metabolism in plants, with increasing evidence for anterograde and retrograde signaling as well as changes in mitochondrial function that can impact plant defenses [17,18,19]. In A. stephensi, p38 MAPK and extracellular signal-regulated kinase (ERK MAPK) are involved in pathogen sensing and NOS activation [20, 21]. Both ERK and p38 MAPK are known to be regulated by transforming growth factor-β-associated kinase 1 (TAK1), a MAP3K that also regulates NOS activation in A. stephensi [22,23,24,25]. Given that IIS and MAPK signaling control metabolism and mitochondrial function in A. stephensi, ABA signaling through these pathways could coordinately regulate defense, lifespan, and reproduction in the mosquito host.
Lifespan is a determinant of the total number of eggs laid by a female mosquito and the probability that she will survive the extrinsic incubation period, or the time required for development of infectious sporozoites, following a blood meal. Changes in mosquito lifespan have a fourth order effect on vectorial capacity, with senescence notably increasing this effect size [5, 26, 27]. Reduced egg production yields fewer adults in the next generation, which impacts biting rate and, therefore, parasite transmission. Accordingly, we sought to characterize ABA signaling in A. stephensi and the life history traits that are connected by these cellular pathways to better understand the effects of ABA on this important vector species.
Reagents and chemicals
Anti-phospho-AMPKα Thr172 (#2535), phospho-TAK1 Thr184 (#4537), phospho-GSK-3α/β Ser21/9 (#9331), and phospho-Akt Ser473 (#9271) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-phospho-p70S6K Thr412 (#07–018) and phospho-FOXO Thr32 (#07–694) were purchased from Millipore (Billerica, MA, USA). Anti-phospho-ERK (#M8159) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-phospho-JNK (#44-682G) was purchased from Biosource (Carlsbad, CA, USA). Anti-phospho-p38 MAPK Thr180/Tyr182 (#10009177) was purchased from Cayman Chemical. Anti-GAPDH (#ab36840) was purchased from Abcam (Cambridge, MA, USA). Goat anti-rabbit secondary antibody (#ALI4404) was purchased from Biosource. Rabbit anti-mouse secondary antibody (#A9044) was purchased from Sigma-Aldrich. Morpholinos were purchased from Gene Tools, LLC (Philomath, OR, USA).
Plasmodium falciparum infections
Three- to five-day-old A. stephensi were provided with P. falciparum-infected blood containing 100 nM ABA or a diluent control as previously described . Non-engorged individuals were removed immediately after feeding. Midguts were dissected at various time points for gene expression and protein analysis. Oocysts were counted on dissected midguts stained with 0.5% mercurochrome at 10 days post-feeding.
For TAK1-knockdown experiments mosquitoes were fed a saline-ATP meal with 10 μM A. stephensi TAK1-targeted (5′-GAT CCT TAT TAC GTT TCG CTT CGT A-3′) or control human beta-globin-targeted (5′-CCT CTT ACC TCA GTT ACA ATT TAT A-3′) vivo morpholinos as previously described 3 days before being provided with a P. falciparum-infected blood meal .
Transcript levels of P. falciparum a18s rDNA, pfs16 and pfs25 in infected mosquito midguts were determined by qRT-PCR . Data were normalized to transcript levels for A. stephensi ribosomal s7 protein, a18s rDNA, and control levels as previously described . nos, defensin, apl1, tep1, and lrim transcript levels were determined by qRT-PCR and analyzed as described previously .
Western blotting assays
Western blots were prepared and analyzed as described . Briefly, proteins were extracted from 10 pooled midguts, separated by gel electrophoresis on a 10% sodium dodecyl sulfate-polyacrylamide gel, transferred onto a nitrocellulose membrane (BioRad, Hercules, CA, USA) and blocked in 5% dry milk in Tris-buffered saline-0.1% Tween 20 (TBST) for 1 h at room temperature. Membranes were incubated overnight at 4 °C in primary (1°) and secondary (2°) antibodies diluted in 5% non-fat dry milk/TBST as follows: 1:1000 phospho-TAK1 and 1:10,000 goat anti-rabbit IgG; 1:1000 phospho-GSK-3 and 1;10,000 goat anti-rabbit IgG; 1:1000 phospho-p70S6K and 1:2000 goat anti-rabbit IgG; 1:1000 phospho-FOXO and 1:2000 goat anti-rabbit IgG; 1:1000 phospho-Akt and 1:5000 goat-anti-rabbit IgG; 1:10,000 phospho-ERK and 1:20,000 rabbit anti-mouse IgG; 1:1250 phospho-JNK and 1:20,000 goat anti-rabbit IgG; 1:1250 phospho-p38 MAPK and 1:20,000 goat-anti-rabbit IgG; 1:10,000 anti-GAPDH and 1:20,000 goat-anti-rabbit IgG. For detection of phospho-AMPK, membranes were incubated in 1:1000 goat anti-rabbit IgG in 5% milk (TBST) for 2 h at room temperature before imaging. All data were normalized to GAPDH levels in the same samples and to target protein levels in matched control midguts.
Lifespan and fecundity measurements
Uninfected female A. stephensi were given the opportunity to bloodfeed once per week on a 1:1 vol: vol mixture of washed human red blood cells (RBCs) and phosphate-buffered saline (PBS) supplemented with 100 nM ABA or with an equivalent volume of diluent control. Mosquitoes were maintained on 10% sucrose solution and allowed to oviposit once per week. Dead individuals were removed and counted three times per week. For analyses of lifespan under nutrient stress, mosquitoes were treated as above but maintained on 3% sucrose solution between weekly blood meals.
To analyze the effects of ABA on infected lifespan, 3–5 day old mosquitoes were provided with a P. falciparum-infected blood meal supplemented with 100 nM ABA or with an equivalent volume of diluent control. Mosquitoes that did not fully engorge were removed from the cartons. For the remainder of the infected lifespan study, mosquitoes were given the opportunity to feed once per week on uninfected blood with or without ABA. Mosquitoes were allowed to oviposit once per week and maintained on 10% sucrose solution between weekly blood meals. Dead individuals were counted every 24 h and each dead individual was placed in Trizol for subsequent RNA isolation. Infection status of each mosquito was determined by detection of transcript levels of P. falciparum mitochondrial cytochrome c oxidase subunit 1 (Pfcox1) gene (forward: 5′-TGC CAG GAT TAT TCG GAG GA-3′; reverse: 5′-CCA TCC AGT TCC ACC ACC AA-3′) by qRT-PCR .
For fecundity studies, 3–5 day old female A. stephensi were fed on uninfected RBCs in PBS (1:1 vol: vol) supplemented with or without 100 nM ABA. Fully engorged females were housed individually and provided oviposition cups at 2 days post-feeding. Two days after the provision of oviposition cups, eggs were counted and prepared for hatching in individual water cups. First instar larvae were provided with a 2% solution of 2:1 Sera Micron® powdered fish food (Sera North America, Montgomeryville, PA, USA) and baker’s yeast. First instar larvae were counted at 2 days after hatching.
Levels of phosphorylated proteins were analyzed by Student’s t-test. Nos transcript levels were analyzed by Wilcoxon matched-pairs signed rank test. All other qRT-PCR data were analyzed by Student’s t-test. Infection prevalence, egg laying rate, and egg hatch rate were analyzed using Fisher’s exact test. Median survival rate and clutch size were analyzed by Wilcoxon matched-pairs signed rank test and Student’s t-test, respectively. Lifespan data were analyzed using log rank test and Gehan-Breslow-Wilcoxon test. Differences were considered significant at P < 0.05.
Sexual stage commitment of P. falciparum is restricted by ABA treatment within 24 h of mosquito infection
The P. falciparum genes pfs16 and pfs25 are expressed in early gametocytes through zygotes and in gametocytes through oocyst stages, respectively [30, 31]. In ABA-treated mosquitoes, expression levels of pfs16 trended downward and pfs25 transcript levels were significantly lower than controls by 24 h post-infection (t = 2.311, df = 3, P = 0.05), suggesting that ABA decreased parasite numbers between 18 and 24 h post-infection, prior to parasite invasion of the midgut epithelium (Fig. 1) .
ABA reduced Plasmodium infection in the mosquito by increasing TAK1-regulated host immunity
We previously showed that ABA supplementation increased expression of defensin, apl1, and nos in the midgut of A. stephensi within hours of blood-feeding. These genes are regulated by multiple pathways, including those that are IIS-, MAPK- and NF-κB-dependent [12,13,14, 20, 21]. To determine whether ABA activates these pathways to regulate host immunity, we examined insulin-like peptide (ilp) gene expression, which is regulated by IIS , as well as activation of the MAPKs ERK, JNK, and p38 MAPK and the MAP3K TAK1 in the A. stephensi midgut. We focused our analyses on ILP3 and ILP4, which regulate P. falciparum development in A. stephensi through effects on midgut intermediary metabolism and mitochondrial function . In contrast to ABA enhancement of insulin secretion in mammalian cells, ABA significantly repressed expression of ilp3 mRNA at 8 h post-infection (t = 2.953, df = 6, P = 0.026) and ilp4 mRNA at 4 (t = 3.472, df = 5, P = 0.018), 6 (t = 13.42, df = 5, P < 0.0001), and 8 (t = 3.079, df = 6, P = 0.022) hours post-infection in the mosquito midgut relative to controls (Fig. 2). This repression was consistent with ABA’s effect on infection given that knockdown of ILP3 and ILP4 is also associated with reduced P. falciparum infection in A. stephensi . Addition of 100 nM ABA to a P. falciparum-infected blood meal had no effect on phosphorylation of ERK, JNK or p38 MAPK relative to controls (Fig. 3, Additional file 1: Figure S1), but ABA treatment rapidly increased phosphorylation of TAK1 with a significant 3-fold mean increase occurring within 30 min post-infection (t = 1.804, df = 9, P = 0.05) (Fig. 4a, Additional file 1: Figure S1).
To determine whether TAK1 phosphorylation mediated ABA-dependent immune gene expression and the effects of ABA on P. falciparum infection, we used a knockdown strategy based on supplementation of an infected blood meal with TAK1-targeted morpholino. Although phosphorylated TAK1 is expected to constitute a relatively small proportion of total TAK1 protein, TAK1 morpholino-treated, infected A. stephensi had significantly reduced midgut phospho-TAK1 levels in response to ABA relative to infected mosquitoes treated with control morpholino (Mann-Whitney test, U = 1, n 1 = 4, n 2 = 4, P = 0.029) (Fig. 4b), confirming TAK1 knockdown. In TAK1 morpholino-treated, infected mosquitoes, ABA-dependent nos expression trended downward (Wilcoxon test, W = -10, P = 0.06) (Fig. 4c) and expression levels of apl1 (t = 4.074, df = 4, P = 0.008), lrim (t = 2.23, df = 4, P = 0.023), and tep1 (t = 3.02, df = 4, P = 0.039) were significantly reduced relative to controls by 6–8 h post-infection (Fig. 4d). defensin expression, which was only modestly induced by ABA in earlier studies, was not altered by TAK1 knockdown (Fig. 4d).
These observations suggested that ABA signaling of TAK1 phosphorylation controlled NF-κB-dependent immunity in A. stephensi. To link this signaling to P. falciparum infection, we examined infection prevalence (the presence of at least one oocyst) in TAK1 morpholino-treated and control mosquitoes with and without ABA supplementation. In control morpholino-treated mosquitoes, ABA supplementation as expected significantly reduced infection prevalence relative to mosquitoes not treated with ABA (Fisher’s exact test, P = 0.001) (Fig. 5a). In TAK1 morpholino-treated mosquitoes, however, ABA supplementation did not significantly alter infection prevalence compared to controls that were not treated with ABA (Fig. 5b). Taken together, these observations suggested that ABA-induced TAK1 phosphorylation mediates ABA repression of P. falciparum infection. Importantly, infection prevalences in both control morpholino-treated and TAK1 morpholino-treated mosquitoes in the absence of ABA were not significantly different from control mosquitoes that were not fed morpholinos (Additional file 2: Figure S2). In the absence of ABA treatment, infection levels in TAK1 morpholino-treated mosquitoes trended lower than those in mosquitoes treated with control morpholinos (Additional file 2: Figure S2). These observations suggested that reducing TAK1 may activate a compensatory immune response that is independent of the ABA-induced response.
ABA transiently altered metabolism and homeostasis in the mosquito midgut
While ABA repression of ilp3 and ilp4 transcript levels in the A. stephensi midgut was consistent with the effects of ABA on P. falciparum infection, our previous studies of these peptides  as well as observed effects of ABA on mammals and plants [33,34,35,36] suggested that ABA may have broader effects on A. stephensi physiology. A major target of IIS is phosphorylation and inhibition of GSK-3, a pivotal mediator of metabolism, inflammation, cell death, and survival in mammalian cells . Given that IIS is activated in the mosquito midgut within minutes by P. falciparum glycosylphosphatidylinositols (GPIs) , it was not surprising to see a trend towards increased inhibitory phosphorylation of GSK-3 in the midgut within 30 min of feeding on P. falciparum-infected blood with ABA (Fig. 6, Additional file 1: Figure S1). However, by 1 h post-feeding, GSK-3 inhibitory phosphorylation was significantly repressed in the midgut of ABA-treated mosquitoes relative to controls (t = 3.897, df = 3, P = 0.03), suggesting a shift to positive regulation of GSK-3 activity by ABA as has been observed in plants .
GSK-3 functions as a monitor of cellular energy status and under energy-rich conditions promotes dephosphorylation of AMP kinase (AMPK) at Thr172 , which inhibits AMPK activity. Inhibition of AMPK prevents the activation of catabolic pathways that generate ATP, the induction of mitochondrial biogenesis, and the inhibition of biosynthetic pathways that consume ATP. Accordingly, we examined AMPK in the midgut and found that Thr172 phosphorylation was significantly reduced in ABA-treated A. stephensi relative to controls at both 30 min (t = 3.145, df = 5, P = 0.0175) and 4 h post-feeding (t = 3.852, df = 2, P = 0.03) (Fig. 6, Additional file 1: Figure S1). Sustained inactivation or deficiency of AMPK promotes mitochondrial dysfunction, which can lead to elevated levels of mitochondrial reactive oxygen species (ROS) and inflammatory levels of NO [41, 42]. ABA-TAK1 signaling (Fig. 4) and AMPK inhibition (Fig. 6), therefore, could additively generate high levels of NO that, based on our previous observations , would be damaging to cell health and autophagic repair in the midgut epithelium.
Based on these suppositions, we examined readouts of midgut epithelial homeostasis [10, 11, 14] in P. falciparum-infected A. stephensi with and without ABA supplementation. We measured transcript levels of prospero and escargot, markers of cell proliferation and differentiation, respectively, as well as atg6 and atg8, markers of autophagosome initiation and maturation, respectively [11, 14, 43,44,45]. Collectively, we have linked changes in thee markers to changes in ROS levels and midgut barrier integrity in the context of insulin-like growth factor treatment and Akt overexpression [11, 14]. In accordance with our observations of the transient effects on midgut signaling and gene expression (Figs. 2, 4), ABA significantly reduced expression of prospero (t = 9.26, df = 2, P = 0.0115) and escargot (t = 4.689, df = 2, P = 0.0426) at 48 h post-feeding and of atg6 and atg8 from 48 to 72 h (t = 9.181, df = 3, P = 0.0027; t = 3.938, df = 3, P = 0.0292) and 24–72 h post-feeding (t = 14.82, df = 2, P = 0.0045; t = 8.126, df = 3, P = 0.0039; t = 3.329, df = 3, P = 0.0447), respectively, with a return to baseline levels by 96 h post-feeding (Fig. 7).
ABA does not alter mosquito lifespan or fecundity
Although the effects of ABA on the midgut were transient, they were associated with a significant inflammatory response and notable effects on biomarkers of cellular homeostasis. Accordingly, we investigated the effect of ABA on mosquito lifespan and fecundity in a series of studies. Provision of 100 nM ABA in weekly, uninfected blood meals with maintenance between blood meals on 10% sucrose did not alter median mosquito survival although there was some variation among cohorts of mosquitoes (Fig. 8, Table 1, Additional file 3: Figure S3). Though ABA had no effect on lifespan under these conditions, we reasoned that ABA could alter lifespan when combined with nutrient stress (3% sugar) between blood meals [46, 47]. However, ABA had no effect on uninfected lifespan under nutrient stress (Fig. 9a).
Extension of infected lifespan can increase parasite transmission, but the relationship between age-dependent mortality and infection is complex . Accordingly, we conducted a lifespan study in which the first blood meal contained P. falciparum-infected RBCs with and without 100 nM ABA. Subsequent weekly blood meals were uninfected with and without ABA. As individual mosquitoes died they were collected and tested for parasite infection by detection of Pfcox1 by qRT-PCR. Mosquitoes that did not have detectable levels of Pfcox1 were considered uninfected and not included in the analysis. Even in the presence of parasite infection, however, ABA supplementation had no significant effect on the lifespan of A. stephensi (Fig. 9b).
In addition to lifespan, mosquito population size can have a large impact on disease transmission. We reasoned that the changes in ilp expression induced by ABA could have an effect on fecundity as observed in Aedes aegypti  and based on observations that injections of ABA reduced vitellogenesis in the flesh fly Sarcophaga bullata . However, ABA had no effect on egg laying rate, clutch size, and egg hatch rate relative to unsupplemented control A. stephensi following a single uninfected blood meal (Fig. 10).
The effects of ABA ingestion on mosquito midgut signaling and gene expression are both notable and transient (Fig. 11). An early transient response to ABA is also seen in plants where signaling through pathways, including the MAPK cascade, is induced within minutes of treatment and peaks within a few hours, followed by later transcriptional changes that control stress responses by maintaining redox and water balance . Factors that terminate ABA signaling in plants are poorly understood, although target-specific protein degradation appears to contribute to downregulation of induced stress responses . Although it is unclear whether inhibitory signaling cascades feedback to inhibit ABA signaling in A. stephensi or if these short-lived responses reflect limited retention of ABA or degradation of ABA or induced target proteins, the biological effects of ABA on the mosquito are nonetheless significant.
Within 30 min to 1 h post-feeding, ABA induced changes in phosphorylation and activity of major immune and metabolic regulators in the mosquito midgut, including TAK1, AMPK, and GSK-3. ABA supplementation increased levels of phosphorylated TAK1, a positive regulator of NF-κB signaling, and TAK1 knockdown in the mosquito midgut abrogated the effects of ABA on immune gene expression and P. falciparum infection prevalence. These data support the hypothesis that ABA enhances anti-parasite defenses by increasing TAK1-dependent expression of NF-κB-regulated immune genes, including nos. These data are also consistent with findings in mammalian cells, where ABA treatment can enhance NF-κB nuclear translocation and transcriptional activity in multiple cell types [53,54,55].
The changes in metabolic protein activity and gene expression in response to ABA suggest a temporary shift in bioenergetic regulation within the midgut that facilitates and perhaps enhances parasite clearance. Specifically, a reduction in AMPK phosphorylation within 30 min post-ingestion of ABA can promote mitochondrial NO production [41, 42], an effective anti-parasite defense in A. stephensi . Further, ABA-dependent activation of GSK3 by 1 h post-feeding would be predicted to sustain dephosphorylation of AMPK, enhancing NF-κB activity and NO production through alternate signaling pathways [40, 56,57,58,59,60,61]. Combined, these predicted synergistic effects on NO synthesis would support effective killing of P. falciparum sexual stages prior to invasion of the midgut epithelium. The repression of AMPK activity is also consistent with the repression of autophagy  observed 24–72 h post-feeding (Fig. 7). Interestingly, inhibition of autophagy can increase caspase-dependent apoptotic cell death [63, 64]. Together with high levels of NO synthesis, an increase in apoptosis in the midgut epithelium during ookinete invasion would be predicted to additively reduce oocyst formation in the A. stephensi midgut .
We have observed that inflammatory NO synthesis can also damage the A. stephensi midgut epithelium, with concomitant reductions in stem cell proliferation and differentiation (escargot, prospero). Increased NF-κB activity has also been linked with decreased expression of autophagy-related genes as well as decreased cell renewal and differentiation [66, 67], suggesting multiple mechanisms whereby ABA could alter midgut homeostasis. In A. stephensi engineered to overexpress Akt under a midgut-specific promoter , damage to midgut mitochondrial function and epithelial homeostasis was profound and sustained, resulting in a significant reduction in lifespan. Here we saw no effect of ingested ABA on lifespan, even under the stresses of nutrient limitation and P. falciparum infection, most likely because the effects of ABA on signaling and gene expression were transient. Similarly, ABA had no effect on egg laying or egg viability within the first gonotrophic cycle, providing further evidence that transient enhancement of host defenses by ABA does not significantly alter fitness. Importantly, since lifespan and reproduction in the first gonotrophic are also not increased, the use of ABA as a therapeutic with transmission blocking activity would not be expected to increase vectorial capacity in mosquitoes that might feed on treated individuals.
Overall, our studies have demonstrated that the effects of ABA on mosquito immunity and key metabolic regulatory kinases are consistent with effects that have been reported in plants and in mammals. Interestingly, in plants ABA can activate or inhibit AMPK signaling in a tissue dependent manner , suggesting that the effects of ABA in the mosquito could vary across tissues. Further, pro- and anti-inflammatory effects of ABA have been reported for acute infection and chronic diseases, respectively, in mammals  suggesting that the effects of ABA in mosquitoes could vary depending on the nature of the infecting pathogen or in response to changes in associated microbiota.
ABA is a highly conserved regulator of immune and metabolic homeostasis within the malaria vector A. stephensi with potential as a transmission-blocking supplemental treatment. Further investigation of ABA signaling will help determine its potential efficacy as a transmission-blocking antimalarial therapeutic and increase our understanding of a highly conserved mediator of immune and metabolic homeostasis.
Glennon EKK, Adams LG, Hicks DR, Dehesh K, Luckhart S. Supplementation with abscisic acid reduces malaria disease severity and parasite transmission. Am J Trop Med Hyg. 2016;96:1266–75.
Pakpour N, Riehle MA, Luckhart S. Effects of ingested vertebrate-derived factors on insect immune responses. Curr Opin Insect Sci. 2014;3:1–5.
Luckhart S, Pakpour N, Giulivi C. Host-pathogen interactions in malaria: cross-kingdom signaling and mitochondrial regulation. Curr Opin Immunol. 2015;36:73–9.
Pakpour N, Akman-Anderson L, Vodovotz Y, Luckhart S. The effects of ingested mammalian blood factors on vector arthropod immunity and physiology. Microbes Infect. 2013;15:243–54.
Brady OJ, Godfray HC, Tatem AJ, Gething PW, Cohen JM, McKenzie FE, et al. Vectorial capacity and vector control: reconsidering sensitivity to parameters for malaria elimination. Trans R Soc Trop Med Hyg. 2016;110:107–17.
Bruzzone S, Bodrato N, Usai C, Guida L, Moreschi I, Nano R, et al. Abscisic acid is an endogenous stimulator of insulin release from human pancreatic islets with cyclic ADP ribose as second messenger. J Biol Chem. 2008;283:32188–97.
Kang MA, Mott TM, Tapley EC, Lewis EE, Luckhart S. Insulin regulates aging and oxidative stress in Anopheles stephensi. J Exp Biol. 2008;211:741–8.
Pakpour N, Corby-Harris V, Green GP, Smithers HM, Cheung KW, Riehle MA, Luckhart S. Ingested human insulin inhibits the mosquito NF-κB-dependent immune response to Plasmodium falciparum. Infect Immun. 2012;80:2141–9.
Drexler A, Nuss A, Hauck E, Glennon E, Cheung K, Brown M, Luckhart S. Human IGF1 extends lifespan and enhances resistance to Plasmodium falciparum infection in the malaria vector Anopheles stephensi. J Exp Biol. 2013;216:208–17.
Hauck ES, Antonova-Koch Y, Drexler A, Pietri J, Pakpour N, Liu D, et al. Overexpression of phosphatase and tensin homolog improves fitness and decreases Plasmodium falciparum development in Anopheles stephensi. Microbes Infect. 2013;15:775–87.
Drexler AL, Pietri JE, Pakpour N, Hauck E, Wang B, Glennon EK, et al. Human IGF1 regulates midgut oxidative stress and epithelial homeostasis to balance lifespan and Plasmodium falciparum resistance in Anopheles stephensi. PLoS Pathog. 2014;10:e1004231.
Pietri JE, Pietri EJ, Potts R, Riehle MA, Luckhart S. Plasmodium falciparum suppresses the host immune response by inducing the synthesis of insulin-like peptides (ILPs) in the mosquito Anopheles stephensi. Dev Comp Immunol. 2015;53:134–44.
Pietri JE, Pakpour N, Napoli E, Song G, Pietri E, Potts R, et al. Two insulin-like peptides differentially regulate malaria parasite infection in the mosquito through effects on intermediary metabolism. Biochem J. 2016;473:3487–503.
Luckhart S, Giulivi C, Drexler AL, Antonova-Koch Y, Sakaguchi D, Napoli E, et al. Sustained activation of Akt elicits mitochondrial dysfunction to block Plasmodium falciparum infection in the mosquito host. PLoS Pathog. 2013;9:e1003180.
de Zelicourt A, Colcombet J, Hirt H. The role of MAPK modules and ABA during abiotic stress signaling. Trends Plant Sci. 2016;21:677–85.
Woo HR, Kim HJ, Nam HG, Lim PO. Plant leaf senescence and death - regulation by multiple layers of control and implications for aging in general. J Cell Sci. 2013;126:4823–33.
Berkowitz O, De Clercq I, Van Breusegem F, Whelan J. Interaction between hormonal and mitochondrial signalling during growth, development and in plant defence responses. Plant Cell Environ. 2016;39:1127–39.
Colombatti F, Gonzalez DH, Welchen E. Plant mitochondria under pathogen attack: a sigh of relief or a last breath? Mitochondrion. 2014;19:238–44.
Fuchs R, Kopischke M, Klapprodt C, Hause G, Meyer AJ, Schwarzländer M, et al. Immobilized subpopulations of leaf epidermal mitochondria mediate PENETRATION2-dependent pathogen entry control in Arabidopsis. Plant Cell. 2016;28:130–45.
Wang B, Pakpour N, Napoli E, Drexler A, Glennon EK, Surachetpong W, et al. Anopheles stephensi p38 MAPK signaling regulates innate immunity and bioenergetics during Plasmodium falciparum infection. Parasit Vectors. 2015;8:424.
Surachetpong W, Singh N, Cheung KW, Luckhart S. MAPK ERK signaling regulates the TGF-beta1-dependent mosquito response to Plasmodium falciparum. PLoS Pathog. 2009;5:e1000366.
Lee J, Mira-Arbibe L, Ulevitch RJ. TAK1 regulates multiple protein kinase cascades activated by bacterial lipopolysaccharide. J Leukoc Biol. 2000;68:909–15.
Sato S, Sanjo H, Takeda K, Ninomiya-Tsuji J, Yamamoto M, Kawai T, et al. Essential function for the kinase TAK1 in innate and adaptive immune responses. Nat Immunol. 2005;6:1087–95.
Shim JH, Xiao C, Paschal AE, Bailey ST, Rao P, Hayden MS, et al. TAK1, but not TAB1 or TAB2, plays an essential role in multiple signaling pathways in vivo. Genes Dev. 2005;19:2668–81.
Lim J. Induction of Anopheles stephensi nitric oxide synthase by Plasmodium-derived factor (s), PhD dissertation. Blackburg, VA: Virginia Polytechnical Institute; 2004. https://theses.lib.vt.edu/theses/available/etd-11082004-023754/
Novoseltsev VN, Michalski AI, Novoseltseva JA, Yashin AI, Carey JR, Ellis AM. An age-structured extension to the vectorial capacity model. PLoS One. 2012;7:e39479.
Styer LM, Carey JR, Wang JL, Scott TW. Mosquitoes do senesce: departure from the paradigm of constant mortality. Am J Trop Med Hyg. 2007;76:111–7.
Pietri JE, Cheung KW, Luckhart S. Knockdown of mitogen-activated protein kinase (MAPK) signaling in the midgut of Anopheles stephensi mosquitoes using antisense morpholinos. Insect Mol Biol. 2014;23:558–65.
Boissière A, Gimonneau G, Tchioffo MT, Abate L, Bayibeki A, Awono-Ambéné PH, et al. Application of qPCR assay in the investigation susceptibility to malaria infection of the M and S molecular forms of An. gambiae s.s. In Cameroon. PLoS One. 2013;8:e54820.
Moreira CK, Marrelli MT, Jacobs-Lorena M. Gene expression in Plasmodium: from gametocytes to sporozoites. Int J Parasitol. 2004;34:1431–40.
Berry A, Deymier C, Sertorio M, Witkowski B, Benoit-Vical F. Pfs 16 pivotal role in Plasmodium falciparum gametocytogenesis: a potential antiplasmodial drug target. Exp Parasitol. 2009;121:189–92.
Meis JF, Ponnudurai T. Ultrastructural studies on the interaction of Plasmodium falciparum ookinetes with the midgut epithelium of Anopheles stephensi mosquitoes. Parasitol Res. 1987;73:500–6.
Magnone M, Ameri P, Salis A, Andraghetti G, Emionite L, Murialdo G, et al. Microgram amounts of abscisic acid in fruit extracts improve glucose tolerance and reduce insulinemia in rats and in humans. FASEB J. 2015;29:4783–93.
Qi CC, Zhang Z, Fang H, Liu J, Zhou N, Ge JF, et al. Antidepressant effects of abscisic acid mediated by the downregulation of corticotrophin-releasing hormone gene expression in rats. Int J Neuropsychopharmacol. 2014;18:pyu006.
Leon J, Castillo MC, Coego A, Lozano-Juste J, Mir R. Diverse functional interactions between nitric oxide and abscisic acid in plant development and responses to stress. J Exp Bot. 2014;65:907–21.
Gomez-Cadenas A, Vives V, Zandalinas SI, Manzi M, Sanchez-Perez AM, Perez-Clemente RM, Arbona V. Abscisic acid: a versatile phytohormone in plant signaling and beyond. Curr Protein Pept Sci. 2015;16:413–34.
Maurer U, Preiss F, Brauns-Schubert P, Schlicher L, Charvet C. GSK-3 - at the crossroads of cell death and survival. J Cell Sci. 2014;127:1369–78.
Lim J, Gowda DC, Krishnegowda G, Luckhart S. Induction of nitric oxide synthase in Anopheles stephensi by Plasmodium falciparum: mechanism of signaling and the role of parasite glycosylphosphatidylinositols. Infect Immun. 2005;73:2778–89.
Youn JH, Kim TW. Functional insights of plant GSK3-like kinases: multi-taskers in diverse cellular signal transduction pathways. Mol Plant. 2015;8:552–65.
Suzuki T, Bridges D, Nakada D, Skiniotis G, Morrison SJ, Lin JD, et al. Inhibition of AMPK catabolic action by GSK3. Mol Cell. 2013;50:407–19.
Singh J, Suhail H, Giri S. Loss of AMP-activated protein kinase induces mitochondrial dysfunction and proinflammatory response in unstimulated Abcd1-knockout mice mixed glial cells. Mediat Inflamm. 2015;2015:176983.
Zaha VG, Qi D, Su KN, Palmeri M, Lee HY, Hu X, et al. AMPK is critical for mitochondrial function during reperfusion after myocardial ischemia. J Mol Cell Cardiol. 2016;91:104–13.
Micchelli CA, Perrimon N. Evidence that stem cells reside in the adult Drosophila midgut epithelium. Nature. 2005;439:475–9.
Ohlstein B, Spradling A. The adult Drosophila posterior midgut is maintained by pluripotent stem cells. Nature. 2005;439:470–4.
Suzuki K, Ohsumi Y. Molecular machinery of autophagosome formation in yeast Saccharomyces cerevisiae. FEBS Lett. 2007;581:2156–61.
Clifton ME, Noriega FG. Nutrient limitation results in juvenile hormone-mediated resorption of previtellogenic ovarian follicles in mosquitoes. J Insect Physiol. 2011;57:1274–81.
Canyon DV, Hii JLK, Muller R. Effect of diet on biting, oviposition, and survival of Aedes aegypti (Diptera: Culicidae). J Med Entomol. 1999;36:301–8.
Dawes EJ, Churcher TS, Zhuang S, Sinden S, Sinden RE, Basáñez MG. Anopheles mortality is both age- and Plasmodium-density dependent: implications for malaria transmission. Malar J. 2009;8:228.
Brown MR, Clark KD, Gulia M, Zhao Z, Garczynski SF, Crim JW, et al. An insulin-like peptide regulates egg maturation and metabolism in the mosquito Aedes aegypti. Proc Natl Acad Sci USA. 2008;105:5716–21.
De Man W, De Loof A, Briers T, Huybrechts R. Effect of abscisic acid on vitellogenesis in Sarcophaga bullata. Entomol Exp Appl. 1981;29:259–67.
Finkelstein RR, Rock CD. Abscisic acid biosynthesis and response. Arabidopsis Book. 2002;1:e0058.
Vanhee C, Zapotoczny G, Masquelier D, Ghislain M, Batoko H. The Arabidopsis multistress regulator TSPO is a heme binding membrane protein and a potential scavenger of porphyrins via an autophagy-dependent degradation mechanism. Plant Cell. 2011;23:785–805.
Magnone M, Bruzzone S, Guida L, Damonte G, Millo E, Scarfi S, et al. Abscisic acid released by human monocytes activates monocytes and vascular smooth muscle cell responses involved in atherogenesis. J Biol Chem. 2009;284:17808–18.
Magnone M, Sturla L, Jacchetti E, Scarfi S, Bruzzone S, Usai C, et al. Autocrine abscisic acid plays a key role in quartz-induced macrophage activation. FASEB J. 2012;26:1261–71.
Scarfi S, Fresia C, Ferraris C, Bruzzone S, Fruscione F, Usai C, et al. The plant hormone abscisic acid stimulates the proliferation of human hemopoietic progenitors through the second messenger cyclic ADP-ribose. Stem Cells. 2009;27:2469–77.
Medunjanin S, Schleithoff L, Fiegehenn C, Weinert S, Zuschratter W, Braun-Dullaeus RC. GSK-3β controls NF-kappaB activity via IKKγ/NEMO. Sci Rep. 2016;6:38553.
Salminen A, Hyttinen JM, Kaarniranta K. AMPK-activated protein kinase inhibits NF-κB signaling and inflammation: impact on healthspan and lifespan. J Mol Med. 2011;89:667–76.
Beurel E, Michalek SM, Jope RS. Innate and adaptive immune responses regulated by glycogen synthase kinase-3 (GSK3). Trends Immunol. 2010;31:24–31.
Chang YT, Chen CL, Lin CF, Lu SL, Cheng MH, Kuo CF, Lin YS. Regulatory role of GSK-3β on NF-κB, nitric oxide, and TNF-α in group a streptococcal infection. Mediat Inflamm. 2013;2013:720689.
Yuskaitis CJ, Jope RS. Glycogen synthase kinase-3 regulates microglial migration, inflammation, and inflammation-induced neurotoxicity. Cell Signal. 2009;21:264–73.
Chen H, Yang S, Yang Z, Ma L, Jiang D, Mao J, et al. Inhibition of GSK-3beta decreases NF-kappaB-dependent gene expression and impairs the rat liver regeneration. J Cell Biochem. 2007;102:1281–9.
Alers S, Löffler AS, Wesselborg S, Stork B. Role of AMPK-mTOR-Ulk1/2 in the regulation of autophagy: cross talk, shortcuts, and feedbacks. Mol Cell Biol. 2012;32:2–11.
Young MM, Takahashi Y, Khan O, Park S, Hori T, Yun J, et al. Autophagosomal membrane serves as platform for intracellular death-inducing signaling complex (iDISC)-mediated caspase-8 activation and apoptosis. J Biol Chem. 2012;287:12455–68.
Amir M, Zhao E, Fontana L, Rosenberg H, Tanaka K, Gao G, Czaja MJ. Inhibition of hepatocyte autophagy increases tumor necrosis factor-dependent liver injury by promoting caspase-8 activation. Cell Death Differ. 2013;20:878–87.
Kakani P, Suman S, Gupta L, Kumar S. Ambivalent outcomes of cell apoptosis: a barrier or blessing in malaria progression. Front Microbiol. 2016;7:302.
Tilstra JS, Clauson CL, Niedernhofer LJ, Robbins PD. NF-κB in aging and disease. Aging Dis. 2011;2:449–65.
Mehrpour M, Esclatine A, Beau I, Codogno P. Overview of macroautophagy regulation in mammalian cells. Cell Res. 2010;20:748–62.
Corby-Harris V, Drexler A, Watkins de Jong L, Antonova Y, Pakpour N, Ziegler R, et al. Activation of Akt signaling reduces the prevalence and intensity of malaria parasite infection and lifespan in Anopheles stephensi mosquitoes. PLoS Pathog. 2010;6:e1001003.
Crozet P, Margalha L, Confraria A, Rodrigues A, Martinho C, Adamo M, et al. Mechanisms of regulation of SNF1/AMPK/SnRK1 protein kinases. Front Plant Sci. 2014;5:190.
Sakthivel P, Sharma N, Klahn P, Gereke M, Bruder D. Abscisic acid: a phytohormone and mammalian cytokine as a novel pharmacon with potential for future development into clinical applications. Curr Med Chem. 2016;23:1549–70.
We thank Kong Wai Cheung for P. falciparum culture maintenance.
Funding for this study was provided by the UC Davis T32 training grant “Animal Models of Infectious Diseases” and by the Floyd and Mary Schwall Dissertation Year Fellowship in Medical Research, awarded to EKKG.
Availability of data and materials
All data generated or analyzed during this study are included in this published article.
Ethics approval and consent to participate
Consent for publication
SL and EKKG are authors on a patent. All other authors have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Representative western blots of phospho-JNK (46 kDa), phospho-ERK (44 kDa), phospho-p38 MAPK (43 kDa), phospho-TAK1 (82 kDa), phospho-AMPK (62 kDa), phospho-GSK3 (46 kDa), and GAPDH (37 kDa) in midguts of mosquitoes at 30 min post-infection with P. falciparum with or without 100 nM ABA. (PDF 872 kb)
P. falciparum infection prevalence in mosquitoes fed no morpholino, a control morpholino, or a TAK1-targeted morpholino. Data were analyzed by Fisher’s exact test (n = 50–90 midguts). (PDF 650 kb)
Survival curves of five lifespan experiments, each conducted with a separate biological cohort of 300 mosquitoes per treatment. Mosquitoes were provided with weekly uninfected bloodmeals containing 100 nM ABA or a diluent control. (PDF 1065 kb)
About this article
Cite this article
Glennon, E.K.K., Torrevillas, B.K., Morrissey, S.F. et al. Abscisic acid induces a transient shift in signaling that enhances NF-κB-mediated parasite killing in the midgut of Anopheles stephensi without reducing lifespan or fecundity. Parasites Vectors 10, 333 (2017) doi:10.1186/s13071-017-2276-4
- Plasmodium falciparum
- Anopheles stephensi
- Abscisic acid
- Nitric oxide
- Innate immunity