- Open Access
Echinococcus granulosus hydatid cyst location is modified by Fasciola hepatica infection in cattle
© The Author(s). 2018
- Received: 26 April 2018
- Accepted: 30 September 2018
- Published: 10 October 2018
Natural parasite infection occurs in wild and domestics animals with more than one parasite species at the same time, generating an infection called polyparasitism. Cystic echinococcosis reports are usually based only on infection with Echinoccocus granulosus leaving aside other internal parasitoses that could modulate both the immune response and pathogenesis of the natural infection. Fasciola hepatica is another cosmopolitan parasite in ruminants with a similar distribution to E. granulosus in different parts of the world, but no information of the effect of co-infection with E. granulosus has been described. The aims of this report were to establish E. granulosus prevalence and explore the association of F. hepatica co-infection and natural E. granulosus infections in cattle.
From 1725 animals, the prevalence of E. granulosus and F. hepatica was 21.16 and 51.3%, respectively. Considering both infections, older cattle (> 4 years) presented higher prevalence compared to younger animals. In E. granulosus-infected cattle, 5.21% had fertile cysts, 71.78% infertile cysts, and in 23.01% cysts were smaller than 1 cm in diameter. Considering cyst location, 39.72% had lungs cysts, 24.72% had liver cysts and 36.94% had cysts in both organs. Cyst location significantly differed between age groups: 44.68% of younger animals had cysts only in the lungs, while older animals presented hydatid cyst in the lungs and liver simultaneously (44.15%). With E. granulosus infection alone, 30.26% of cysts were found in the lungs, 31.79% in the liver and 37.95% in both organs. Regarding the co-infection of E. granulosus with F. hepatica, the proportion was significantly different (P < 0.05) with most animals having cysts only in the lungs (49.41%) and a lower level of liver infection (15.88%). Analyzing organ cyst distribution and F. hepatica absence/presence ratio within each cyst type, small cysts showed the highest difference in ratio.
To the best of our knowledge, this is the first report indicating that F. hepatica co-infection in cattle could be affecting the instate of hydatid cysts in the liver, displacing toward lung localization, suggesting an antagonistic relationship.
- Echinococcus granulosus
- Fasciola hepatica
- Hydatid cyst localization
Co-infection with different parasite species in the same host (also known as polyparasitism) is a well-documented fact in medical, veterinary and zoological literature. Most of the animals that live in the wild and humans of rural areas can be hosts of many concurrent parasite species . However, the synergistic or antagonic relationship that different parasite species can have within the same host remains poorly studied . Helminth parasites are a very diverse group of animals that are classified in four taxonomic groups: nematodes, trematodes, cestodes and acanthocephalans . In cattle, two parasites usually represent a frequent infection: the cestode E. granulosus (sensu lato) and the trematode F. hepatica. Echinococcus granulosus (s.l.) has an indirect life-cycle, with ruminants as intermediate hosts, dogs and other canids as definitive hosts, and humans as dead-end hosts . The metacestode stage called hydatid cysts develops in the viscera (mainly lungs and liver) of the intermediate hosts , causing a disease known as cystic echinococcosis. Fasciola hepatica also has an indirect life-cycle; however, herbivores act as the definitive hosts, with the adult worms located in the bile ducts .
Echinococcus granulosus (s.l.) is composed of E. granulosus (sensu stricto) (genotypes G1-3), E. equinus (genotype G4), E. ortleppi (genotype G5), E. canadensis (genotypes G6-8/G10) and E. felidis (“lion strain”), with E. granulosus (s.s.) being the most commonly distributed worldwide .
Although E. granulosus (s.l.) is able to infect a wide range of mammalian hosts, the metacestode stage has a different capacity to produce protoscoleces, the stage infective to the definitive host. For unknown reasons, the parasite can, in some animals, produce protoscoleces inside the cyst, generating a fertile hydatid cyst, but other animals with cystic echonococcosis possess cysts without protoscoleces called infertile hydatid cysts [8, 9]. Since cyst fertility is associated with the size of the hydatid cyst , there is a subset of hydatid cysts that are too small to be classified as either fertile or infertile. The cellular and molecular mechanisms involved in the process of cyst fertility remain unknown . In cattle, hydatid cyst fertility status ranges from 0 to 96% in different parts of the world [11–22] and infection with F. hepatica is common in many parts of the world. However, there are no studies on relationships between E. granulosus (s.l.) and F. hepatica in co-infections.
Although belonging to different higher-level flatworm taxa, both parasites exhibit common traits regarding their interaction with the mammalian host; as such, serum of animals infected experimentally with E. granulosus (s.l.) can recognize F. gigantica antigens in immunoassays  but there are no specific data on cross-reaction with F. hepatica. However, both parasites have the ability to uptake host glycolipids  which could explain the latter. Reports of polyparasitism involving E. granulosus (s.l.) are scarce. There is only one report that includes the interaction with Schistosoma mansoni (a trematode), where in murine models simultaneous concomitant infection lead to higher IFN-γ profiles, displaying a TH1 response; however, adding E. granulosus infection seven weeks after S. mansoni infection led to significant lower IL-10 production, changing the immune profile to a TH2 response . Here, we provide the first report that in bovines infected with E. granulosus (s.l.), the presence of co-infection with F. hepatica is associated with changes in the hydatid cysts localization.
A total of 1725 cattle were examined for the presence of hydatid cyst and F. hepatica infection at post-mortem inspection in a Region Metropolitana slaughterhouse, Chile. In routine slaughtering, animals were individually identified, age and sex was recorded, and visceral organs of each animal, mainly the lungs and liver, were visually examined, palpated and incised along with official veterinarian inspectors for the presence of hydatid cysts and F. hepatica.
Fasciola hepatica diagnosis was made either by direct visualization of adult parasites in bile ducts, or by F. hepatica compatible lesions such as enlarged and thickened bile ducts, calcification of bile ducts, black parasitic material and black lymph nodes in the liver: signs of chronic F. hepatica infection.
Suspected cystic samples were removed from the infected organ, placed in separate polythene bags and transported in an isothermal container within 3 h to Universidad Andres Bello Veterinary School for further examination. For hydatid cysts confirmation and fertility determination, cysts were microscopically examined as previously described . Echinococcus granulosus (s.l.) genotyping in hydatid cyst samples was determined as previously reported . Briefly, DNA was extracted from fertile, infertile and small hydatid cysts from both livers and lungs. The cox1 mtDNA was amplified and sequenced, and a 345-nucleotide consensus sequence was used for comparison analysis.
Animals were classified according to their age, hydatid cyst type and location. By age range, individuals were divided into two groups: 4 years-old or younger (≤ 4 years) and over 4 years of age (> 4 years). Cysts were classified into 3 types: small cysts (< 1 cm in diameter); fertile cysts (with protoscoleces); and infertile cysts (> 1 cm in diameter and without protoscoleces). Animals were also separated into 3 groups according to the location of the hydatid cysts: in lungs only; in liver only; and in both organs simultaneously. All groups were also separated by their F. hepatica co-infection status.
Data were recorded in an Excel 2010 datasheet and analyzed with RStudio IDE version 1.0.136 and R version 3.3.3 for statistical associations among variables using a Chi-square test. Logistic regressions were performed using STATA v.12 software (StataCorp, College Station, TX, USA). Statistical significance was considered when P-values were below the 0.05 threshold.
Prevalence of E. granulosus and F. hepatica
Echinococcus granulosus, Fasciola hepatica and co-infection prevalence, per age range classification of slaughtered cattle
EG and FH co-infected
≤ 4 years
> 4 years
Hydatid cyst type, location and genotype
Frequency and percentage of organ localization according to cyst type
Association of hydatid cyst characteristics with animal age and co-infection with F. hepatica
Hydatid cyst anatomical location within age range and Fasciola hepatica co-infection in E. granulosus (s.s.)-infected animals
Host age range
≤ 4 years
> 4 years
≤ 4 years
> 4 years
≤ 4 years
> 4 years
Associations between hydatid cyst with or without Fasciola hepatica co-infection and host variables: age range and cyst location
< 1 cm diameter
≤ 4 years
> 4 yearsa
Association between hydatid cyst location in liver and the presence of Fasciola hepatica co-infection
OR (95% CI)
The prevalence of E. granulosus and F. hepatica in the present study is consistent with the official national slaughter and condemnation data at abattoirs. The prevalence of E. granulosus infection has remained steady since 1995; however, F. hepatica infection has increased [27, 28]. Older animals had a higher prevalence of E. granulosus infection, as reported in other studies [11, 29, 30], and this prevalence in older animals remains regardless of F. hepatica co-infection. This could be due to an increase in time in which the animal can be exposed to E. granulosus . For the prevalence of F. hepatica however, a difference was found only when E. granulosus-infected animals were included; no association was found between age and the proportion of animals infected. Innocent et al.  found an increase of liver condemnation due to F. hepatica in older cattle; however, the group studied had no animals over 30 months of age and disease prevalence was considerably lower .
The location of hydatid cysts varies among studies. In our study, most of the animals had hydatid cysts in their lungs as reported by some studies [14, 29, 33, 34]; however in others, liver was the most commonly affected organ [11, 18, 35]. A recent study in Chile, found that the proportion of organs infected with E. granulosus varied among geographical locations . These authors have shown that of the genotyped cysts, belonging to 47.95% of E. granulosus-infected animals, most were identified as E. granulosus (s.s.) as has previously been reported in cattle in Chile ; this species is also the most common worldwide .
We found that hydatid cysts organ distribution varied with age, a factor not previously considered. Conversely, separating by co-infection with F. hepatica, a difference was found only in F. hepatica co-infected animals, where the proportion of animals with cysts in both liver and lungs was higher in older co-infected animals when compared with younger co-infected animals. As has been reported, older animals could have a higher number of cysts as exposure time is increased , which could also increase the number of organs affected.
Anatomical location was associated with co-infection status, with a decrease in liver-affected animals and an increase in lung-only-affected animals. In E. granulosus, portal circulation has been described as the primary route of infection by oncospheres, with a high tropism for the liver . As F. hepatica in acute and chronic infections can damage the liver , it could be interfering with the establishment of E. granulosus in this organ. In the absence of F. hepatica, small hydatid cysts were mainly located in the liver, whereas when F. hepatica was present, small cysts were found in a larger proportion in the lungs only. Small cysts may represent either immature cysts that could develop into fertile or infertile cysts, or they could be non-viable cysts.
As reported in other studies, we found that cyst fertility significantly varied in different organs [11, 15, 29, 40], with more fertile cysts in the lungs than in other locations. No statistical association was found between cyst type and age or co-infection with F. hepatica, analyzed independently. However, in older cattle there was an association between cyst type and co-infection with F. hepatica. In the presence of the latter, there were more animals with infertile cysts and fewer with small cysts than in single infections.
Cattle cyst fertility reported in this study, is similar to that reported by other authors [11, 15], but noticeably lower than in other studies [13, 18, 33, 34, 41]. Literature data suggest that polyparasitism interactions can alter the site in host used by the parasites. For example, the antagonic relationship between Moniliformis moniliformis and Hymenolepis diminuta, in which when both parasites co-infect the gut of rats, M. moniliformis is able to displace H. diminuta to a less nutrient rich site in the small intestine; this antagonic relationship was host-specific . The fact that there are different viscera affected when both F. hepatica and E. granulosus (s.l.) parasitize the same bovine host could be an example of an antagonic relationship, since the liver is the main organ or site in host that both parasites seek to establish themselves. The mechanisms that could explain how F. hepatica infection can affect the parasitized viscera by E. granulosus (s.l.) remains to be studied, since it could be via immune response modulation or merely the physical condition of the liver tissue after F. hepatica infection that makes it unsuitable for E. granulosus (s.l.) to establish. This could also be a factor that contributes to the low cyst fertility in cattle from Chile, since the liver is usually infected with F. hepatica. One of the limitations of our study is that we worked with natural infections, so we cannot determine the temporality of F. hepatica and E. granulosus infection in cattle, but here we have shown an effect of displacing the hydatid cysts toward the lungs and an increased proportion of small cysts associated with co-infection with F. hepatica.
Cattle naturally co-infected with E. granulosus and F. hepatica have a lower chance of presenting hydatid cysts in the liver with an increased chance for lung cyst localization, especially for smaller hydatid cysts, suggesting that F. hepatica may affect the instate of E. granulosus in the liver. These results suggest that in cattle natural infected with E. granulosus and F. hepatica, both parasites display an antagonistic relationship.
Funding was provided by FONDECYT-Chile No. 1161475 and the Universidad Andres Bello internal project DI-1249-16/RG. The funding bodies had no role in the design of the study, the collection, analysis and interpretation of data, or in writing the manuscript.
Availability of data and materials
All data generated or analyzed during this study are included in this published article.
CS participated in the sample and data collection, analyzed the obtained data and wrote most of the initial draft of the manuscript. CA participated in the sample and data collection and initial data analysis. CH participated in data analysis, wrote part of manuscript and provided critical review of the manuscript. FC acquired samples and provided critical review of the manuscript. MJ acquired samples. MH provided assistance with the statistical analysis of the results. RP did the study design, results analysis and was a major contributor in writing the manuscript. All authors read and approved the final manuscript.
All procedures were approved by the Universidad Andres Bello Bioethics Board (protocol number 016/2016).
Consent for publication
The authors declare that they have no competing interests.
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