Comparative efficacy of BG-Sentinel 2 and CDC-like mosquito traps for monitoring potential malaria vectors in Europe
Parasites & Vectors volume 15, Article number: 160 (2022)
Different trapping devices and attractants are used in the mosquito surveillance programs currently running in Europe. Most of these devices target vector species belonging to the genera Culex or Aedes, and no studies have yet evaluated the effectiveness of different trapping devices for the specific targeting of Anopheles mosquito species, which are potential vectors of malaria in Europe. This study aims to fill this gap in knowledge by comparing the performance of trapping methods that are commonly used in European mosquito surveillance programs for Culex and Aedes for the specific collection of adults of species of the Anopheles maculipennis complex.
The following combinations of traps and attractants were used: (i) BG-Sentinel 2 (BG trap) baited with a BG-Lure cartridge (BG + lure), (ii) BG trap baited with a BG-Lure cartridge and CO2 (BG + lure + CO2), (iii) Centers for Disease Control and Prevention-like trap (CDC trap) baited with CO2 (CDC + CO2), (iv) CDC trap used with light and baited with BG-Lure and CO2 (CDC light + lure + CO2). These combinations were compared in the field using a 4 × 4 Latin square study design. The trial was conducted in two sites in northeastern Italy in 2019. Anopheles species were identified morphologically and a sub-sample of An. maculipennis complex specimens were identified to species level by molecular analysis.
Forty-eight collections were performed on 12 different trapping days at each site, and a total of 1721 An. maculipennis complex specimens were captured. The molecular analysis of a sub-sample comprising 254 specimens identified both Anopheles messeae/Anopheles daciae (n = 103) and Anopheles maculipennis sensu stricto (n = 8) at site 1, while at site 2 only An. messeae/An. daciae (n = 143) was found. The four trapping devices differed with respect to the number of An. messeae/An. daciae captured. More mosquitoes were caught by the BG trap when it was used with additional lures (i.e. BG + lure + CO2) than without the attractant, CO2 [ratioBG+lure vs BG+lure+CO2 = 0.206, 95% confidence interval (CI) 0.101–0.420, P < 0.0001], while no significant differences were observed between CDC + CO2 and CDC light + lure + CO2 (P = 0.321). The addition of CO2 to BG + lure increased the ability of this combination to capture An. messeae/An. daciae by a factor of 4.85, and it also trapped more mosquitoes of other, non-target species (Culex pipiens, ratioBG+lure vs BG+lure+CO2 = 0.119, 95% CI 0.056–0.250, P < 0.0001; Ochlerotatus caspius, ratioBG+lure vs BG+lure+CO2 = 0.035, 95% CI 0.015–0.080, P < 0.0001).
Our results show that both the BG-Sentinel and CDC trap can be used to effectively sample An. messeae/An. daciae, but that the combination of the BG-Sentinel trap with the BG-Lure and CO2 was the most effective means of achieving this. BG + lure + CO2 is considered the best combination for the routine monitoring of host-seeking An. maculipennis complex species such as An. messeae/An. daciae. The BG-Sentinel and CDC traps have value as alternative methods to human landing catches and manual aspiration for the standardized monitoring of Anopheles species in Europe.
Europe has been considered malaria-free by the World Health Organization since 1975, with the interruption of indigenous malaria transmission since that time . Although eradication programs successfully arrested the circulation of malaria in Europe, they failed to eliminate the competent vectors, Anopheles species belonging mainly to the Anopheles maculipennis complex. Several Anopheles species competent for malaria transmission are still present throughout Europe [2,3,4]. The most common and widely distributed species belong to the An. maculipennis complex, which includes several species with morphologically indistinguishable adults. Molecular identification of the species of this complex is paramount because they do not have the same susceptibility to different Plasmodium species [5,6,7].
Following the eradication of malaria in almost all European countries, and due to the limited incidence of the disease in Europe since the 1970s, scientific interest in the An. maculipennis complex has progressively decreased. Consequently, in the last decades, only a few studies assessing the distribution of the European anopheline fauna have been carried out. However, there are still areas with residual malariogenic potential in various European countries, and in Italy in particular [6, 8], and several cases of cryptic malaria were recorded from 2000 to 2018 . In this regard, it is important to monitor and provide regular updates on the presence and distribution of potential malaria vectors through the implementation of systematic and harmonized surveillance programs throughout Europe to promptly identify potential indigenous transmission events.
A recent study gives an update on the current distribution of the An. maculipennis complex in northern Italy, thanks to extensive field sampling and species identification . However, Anopheles mosquitoes were collected mainly by Centers for Disease Control and Prevention (CDC) traps baited with CO2 in that study, which was undertaken in the context of entomological monitoring for West Nile Virus surveillance . Typically, mosquito monitoring and surveillance programs presently underway in many European countries have been devised to target different vectors of disease within the genera Culex or Aedes. These programs are intended to monitor adult female mosquitoes (host-seeking, ovipositing or resting) by using different types of trapping devices and various methods of mosquito attractants (e.g. CO2, heat, olfactory lures, or light) .
The choice of sampling method depends on the target mosquito species and the study goal [13,14,15]. For example, to obtain accurate data on a mosquito population in a given area, the most suitable sampling methods must be chosen in relation to the behavior of the target mosquito species.
In areas where malaria is endemic, major vectors are often anthropophilic, and the use of human landing catches (HLC) remains the most direct and reliable method for evaluating mosquito density, mosquito biting activity and entomological inoculation rate [13, 16,17,18,19]. However, HLC have underlying ethical issues and involve the risk that human volunteers will come into contact with potentially infected vectors . To avoid human contact with mosquitoes, efficient and reliable alternative tools have been developed to monitor and study vectors in areas endemic for malaria [21,22,23,24,25,26].
However, to the best of our knowledge, no European studies have evaluated the effectiveness of different trapping devices that specifically target Anopheles mosquito species. According to the literature, the method most frequently used to catch Anopheles mosquitoes in Europe is manual aspiration of resting adults in indoor shelters . For the An. maculipennis complex, the CDC light trap seems to be an effective device , although for anthropophilic species HLC remain the gold standard [15, 28]; other sampling approaches have been poorly investigated for this complex.
The aim of the present study was to fill this gap in knowledge as follows: (i) compare the sampling performance of different trapping methods (CDC-like and BG-Sentinel traps baited with different combinations of lures and CO2) commonly used in European mosquito surveillance programs to specifically collect adult of the An. maculipennis complex species, (ii) evaluate the influence of the presence of hosts on the effectiveness of the trapping methods, (iii) assess malaria vector presence/absence and their abundance in sampled sites in order to update the Italian data, (iv) compare the efficacies of the tested traps for the capture of other abundant mosquito species (Culex pipiens and Ochlerotatus caspius).
Study area and experimental design
An indication of the most suitable sites for Anopheles mosquitoes in northern Italy was given by the results of a previous study . Based on this, two sites located in the Veneto Region (northeastern Italy) were selected for the present study, in accordance with the following criteria: countryside location, high abundance of Anopheles mosquitoes based on historical data, and the presence of wild/farmed animals. Two ecologically similar sampling sites, both surrounded by paddy fields, with the same elevation and climatic dynamics, but with a different density of farmed animals, were chosen to evaluate the possible influence of potential hosts in close proximity to the traps on captures. The first site was located on a farm (site 1; Verona Province; 45.29055N, 11.018483E), where 22 different animal species were kept (horses, goats, and chickens were among the most abundant species); the second site was an abandoned house in a rural area without farmed animals (site 2; Rovigo Province; 44.947222N, 12.279425E) (Fig. 1). At the farm, human presence was limited almost exclusively to the daytime, and only one person remained on site at night.
A preliminary single aspiration was performed at each site in summer 2018 to confirm Anopheles mosquito presence. Resting mosquitoes were collected indoors by sweeping a CDC Back Pack aspirator (model 1412; John W. Hock, FL) over the walls and ceilings of animal shelters at site 1, and in the abandoned house at site 2 for a 3-min period. Field-collected mosquitoes were stored and identified according to the same methodology described below. In total, 1059 specimens of An. maculipennis complex were collected: 955 at site 1 (farm) and 104 at site 2 (abandoned house). A sub-sample of the collected mosquitoes (n = 160, 16.8% at site 1 and n = 24, 23.1% at site 2) were identified using molecular methods, of which 147 (91.9%) were Anopheles messeae/Anopheles daciae and 13 (8.1%) Anopheles maculipennis sensu stricto (s.s.) at site 1, while 23 (95.8%) were An. messeae/An. daciae and one (4.2%) was An. maculipennis s.s. at site 2.
Trap comparisons were carried out in 2019 from July to September during the period of peak mosquito density (as assessed in previous samplings). Three replicates and 12 total days of collection were conducted at each site (Additional file 1: Table S1) using two different traps with different combinations of three attractants. The two traps used in the test were the BG-Sentinel 2 Mosquito Trap (BG trap; Biogents, Regensburg, Germany), and a Centers for Disease Control and Prevention-like trap [CDC trap; Italian Mosquito Trap (IMT); PeP, Cantu, Italy]. These traps were designed to collect host-seeking mosquitoes by aspiration, but they differ in their mechanisms of attraction and trapping. Both visual and olfactory attractants can be used with these traps, as follows: light (only with the CDC trap), CO2 provided by dry ice, and a BG-Lure cartridge (Biogents). The following combination of traps and attractants were used: (i) BG trap with BG-Lure cartridge (BG + lure), (ii) BG trap with BG-Lure cartridge and CO2 (BG + lure + CO2), (iii) CDC trap baited with CO2 (CDC + CO2), (iv) CDC trap with light and baited with BG-Lure cartridge and CO2 (CDC light + lure + CO2).
CDC traps were hung on low trees or wooden posts (trap opening at approximately 1-m height from the ground) outdoors. When used with the CDC traps, the lure cartridge and light were fixed near the suction fan of the trap. BG traps were placed on the ground following the manufacturer’s instructions, with the trap opening at 40-cm height from the ground. To use CO2 with the BG trap, the same type of thermic cartridge as that used with the CDC trap was placed 20 cm above the trap and filled daily with dry ice. The trap comparison experiment was set up as a 4 × 4 Latin square. At each location, all the traps were placed approximately 50 m from each other at four different sampling points. To eliminate any position-specific effect, all the traps were rotated to the next position every 24 h four times during the trapping cycle, so that each trap occupied every position during the cycle. Every 24 h, in the late afternoon, mosquitoes from each trap were collected, transported in dry ice, and stored at − 20 °C until processed in the laboratory. After mosquito collection, traps were rotated and refilled with dry ice. The same lure cartridges were used for each trapping cycle and were changed for new ones for the following cycle.
Mosquitoes were morphologically identified under a stereomicroscope using taxonomic keys . A representative sub-sample of specimens belonging to the An. maculipennis complex, which had been caught in 2018 by aspiration and in 2019 by trapping, was identified to species level by molecular methods. The sub-samples were randomly chosen from specimens captured every day by each trap at each site (sample) during the whole trapping period up to a maximum of 13 specimens per sample.
Using just one leg from each specimen, DNA was extracted following the animal tissue dilution and storage protocol of the Phire Tissue Direct PCR Master Mix (ThermoFisher Scientific, Waltham, MA). The extracts were analyzed as described in Lühken et al. . Briefly, the assay is a species-specific multiplex quantitative PCR targeting the internal transcribed spacer 2 of ribosomal DNA. It is based on two primer sequences conserved among the species Anopheles atroparvus, Anopheles maculipennis s.s. and Anopheles messeae sensu lato (s.l.), and two different TaqMan probes for the latter species.
As the splitting of An. messeae into the two species An. messeae and An. daciae [31, 32] is not universally accepted, we overcame this controversial issue by adopting the definition An. messeae/An. daciae, without discriminating between the two taxonomic units.
Meteorological and mosquito data
Meteorological data were obtained from two meteorological stations both approximately 7 km from the study sites. Total daily precipitation (Prec.; millimeters) and mean daily temperature (Tmean; degrees Celsius) during the sampling periods were extracted from the web sites of the Agenzia Regionale per la Prevenzione e Protezione Ambientale del Veneto .
For Anopheles collected in 2019, the proportions of An. messeae/An. daciae and An. maculipennis s.s. identified by molecular analysis were compared to the total An. maculipennis s.l. collected by all the traps and the An. messeae/An. daciae proportion calculated. As a result, the statistical analyses (preliminary tests and model building) were performed entirely for An. messeae/An. daciae, which represented almost all of the Anopheles specimens captured. The final model was then applied to the two other most abundant species of the sampled mosquito population (i.e. Cx. pipiens and Oc. caspius) to obtain a more complete overview of the trapping efficacies of the devices.
The efficacy, defined as the number of trapped mosquitoes, of the four different trapping devices was evaluated by a series of statistical tests and a linear model. The first step consisted of univariate analyses to (i) evaluate the devices’ trapping capabilities in catching mosquitoes (Kruskal–Wallis rank sum test), (ii) assess the effect of the capture sites on the number of trapped mosquitoes (Wilcoxon test), (iii) test the trap position effect within the Latin square on captured An. messeae/An. daciae abundance (Kruskal–Wallis rank sum test), and (iv) assess the effects of daily precipitation and mean temperature on the number of trapped An. messeae/An. daciae (univariate linear models). The likelihood ratio test was then used to identify the best final model through the comparison of different nested models built by sequentially adding one or more variables of interest. A generalized linear model (GLM) with a negative binomial error distribution was chosen to model the count of trapped An. messeae/An. daciae, so that overdispersion, which is frequently associated with non-transformed richness data, could be accounted for . The choice of a negative binomial over a Poisson regression was assessed with the help of diagnostic plots and dispersion tests (Additional file 2: Fig. S1). The parameter θ, which can be interpreted as a measure of data dispersion in the calculation of the variance of the negative binomial distribution, was estimated by means of maximum likelihood . In order to obtain a direct estimate of the two categorical independent variables included in the regression, we omitted the estimation of the intercept to allow the model to reparameterize the remaining categorical covariates. Thus, the interpretation of the coefficient estimates could be done directly, as they did not represent the difference between each group and the reference group (i.e. the intercept). As the negative binomial regression uses a log–link function to link the linear combination of the predictors, the coefficients estimates must be interpreted with an additive effect on the log(y) scale, and with a multiplicative effect on the y scale (i.e. when the coefficients are back-transformed through the exponential function). Finally, contrasts among estimated marginal means were computed to evaluate the significant differences among the tested trapping devices, considering a confidence level of 0.95. The final GLM was also done for the two most abundant mosquito species (Cx. pipiens and Oc. caspius) in the sampled population.
All data cleaning and preparation, statistical analyses and model building were conducted using R statistical software version 4.0.5  and the packages ggplot2 , MASS , emmeans  and DHARMa .
A total of 48 captures were performed for each site on 12 different days of collection in 2019, from 9 July to 6 September at site 1 (farm) and from 2 July to 30 August at site 2 (abandoned house) (Additional file 1: Table S1).
Overall, 25,442 mosquitoes belonging to 10 species were caught: 11,514 specimens at site 1, and 13,928 at site 2 (Table 1). An. maculipennis s.l. was the third most abundant taxon collected at both sites, representing 6.8% of trapped mosquitoes. Overall, 1721 specimens of An. maculipennis s.l. were collected, 437 (3.8%) at site 1 (farm) and 1284 (9.2%) at site 2 (abandoned house). A sub-sample of 254 specimens [n = 111 (25.2%) at site 1 and n = 143 (11.1%) at site 2] were identified to species level by molecular analysis. At site 1 (farm), both An. messeae/An. daciae (n = 103) and An. maculipennis s.s. (n = 8) were captured, while at site 2 (abandoned house), only An. messeae/An. daciae was found (n = 143) (Additional file 1: Table S2).
To assess the effects of the type of device, trapping site and Latin square location on the total number of trapped An. messeae/An. daciae, a series of non-parametric statistical tests was performed, following a preliminary analyses of data normality and variance homogeneity (Additional file 3: Table S3). The differences in mosquito abundance for types of trapping devices were significant (H = 10.673, df = 3, P = 0.0136). In particular, the post hoc pairwise Wilcoxon rank sum test revealed a significant difference between BG + lure and BG + lure + CO2 (P = 0.009; Fig. 2b), suggesting that, in terms of total catch, the BG trap performed better when CO2 was added as a lure; no other comparisons between the total number of mosquitoes caught between the different devices were significant. The daily mean number of Anopheles captured was 50.8 at site 1 and 107.0 at site 2. The Wilcoxon test indicated a significantly higher number of trapped An. messeae/An. daciae for site 2 (abandoned house) compared to site 1 (farm) (W = 722.5, P = 0.0016; Fig. 2a). No significant differences were found among the four positions of the Latin square for either site (site 1, H = 4.244, df = 3, P = 0.236; site 2, H = 7.195, df = 3, P = 0.066), indicating that the observed sampling point-specific differences were likely due to chance (Fig. 2c).
There were significant differences between sites and among the four trapping devices with respect to the number of mosquitoes caught. Univariate GLMs were used to assess the effects of daily precipitation and average temperature on the number of trapped An. messeae/An. daciae. Although both variables had a significant effect [coefficient of average temperature (Coef.Tmean) = 0.268, SE = 0.078, P < 0.001; coefficient of daily precipitation (Coef.Prec.) = − 0.067, SE = 0.024, P < 0.01], the likelihood ratio test of the negative binomial models showed that their inclusion did not significantly increase the final goodness of fit of the base model, which included just the catching sites and the device types [base model vs base model + Tmean, likelihood ratio statistic (LRstat) = 1.623, P = 0.202; base model vs base model + Prec., LRstat = 3.736, P = 0.053; base model vs base model + Tmean + Prec., LRstat = 4.086, P = 0.130]. For this reason, Prec. and Tmean were not included in the subsequent analyses.
A bivariate GLM was built with a negative binomial distribution for over-dispersed count data to analyze the effect of the different trapping devices and location of captures, which were both included as fixed effects, on the number of captured An. messeae/An. daciae. The maximum likelihood estimate of the parameter θ for the final model is 0.684, which is indicative of over-dispersed data. Table 2 reports the exponent estimates and their 95% CIs.
The four trapping devices performed differently in capturing An. messeae/An. daciae. In general, a higher number of mosquitoes were caught by the BG-Sentinel and the CDC traps when they were used with the additional lures (i.e. BG + lure + CO2 and CDC light + lure + CO2) compared to the same devices used without any odor attractant. The trapping device with the highest attractiveness for An. messeae/An. daciae appeared to be the BG + lure + CO2 (Coef. = 14.206). Regardless of trapping device, the number of trapped An. messeae/An. daciae was 3.4 times higher at site 2 (abandoned house) than site 1 (farm). To better evaluate the significant differences between the trapping efficacies of the devices, pairwise contrasts were computed (Table 3).
Four of the six pairwise comparisons of trap efficacies showed statistically significant differences: BG + lure vs BG + lure + CO2, BG + lure vs CDC + CO2, BG + lure vs CDC light + lure + CO2 and BG + lure + CO2 vs CDC + CO2. Specifically, BG + lure captured fewer mosquitoes than the other tested devices (ratioBG+lure vs BG+lure+CO2 = 0.206, P < 0.0001; ratioBG+lure vs CDC+CO2 = 0.467, P = 0.038; ratioBG+lure vs CDC light+lure+CO2 = 0.327, P = 0.002). This result can be interpreted as indicating that the addition of CO2 significantly improved the capability of the BG + lure device by 4.85% (i.e. 1/ratioBG+lure vs BG+lure+CO2); using the BG lure with CDC + CO2 did not lead to any significant improvement in terms of the number of captured mosquitoes (P = 0.321). Additional file 4: Tables S4 and S5 show the exponential estimates and the contrasts between trapping devices for the two other most represented mosquito species of the sampled population, Cx. pipiens and Oc. caspius. CDC + CO2 was the best trapping method for Cx. pipiens (Coef. = 378.52, P < 0.001), and the addition of lure and light did not significantly improve the number of specimens collected by it (P = 0.090). However the BG + lure captured 8.4 times more Cx. pipiens when baited with CO2 (1/ratioBG+lure vs BG+lure+CO2, P < 0.0001). Regarding Oc. caspius, both the CDC and BG traps were more effective when baited with additional lures: BG + lure + CO2 trapped 28.7 times more mosquitoes than BG + lure (1/ratioBG+lure vs BG+lure+CO2; P < 0.0001), and CDC light + lure + CO2 caught 3.2 times more mosquitoes than CDC + CO2 (1/ratioCDC+CO2 vs CDC light+lure+CO2; P = 0.005).
We report evidence from a field study that shows that both BG-Sentinel and CDC traps can be effective in sampling An. messeae/An. daciae. Although the BG-Sentinel trap baited with BG-Lure attracted female mosquitoes, the number of mosquitoes captured increased when more than one attractant was added. In general, traps baited with CO2 collected more mosquitoes. The synergistic effect of CO2 when used with other attractants confirms similar evidence from studies undertaken in the field . In the present study, the synergistic effect of CO2 was particularly evident when it was used with the BG-Sentinel trap; this trap is usually only baited with an odor blend, but it caught the highest number of mosquitoes when it was also baited with CO2, i.e. 4.85 times more An. messeae/An. daciae (P < 0.0001) than when it was used without CO2. BG + lure + CO2 was also significantly (2.3 times) more effective than CDC + CO2 (P = 0.022) in collecting An. messeae/An. daciae. The BG-Sentinel trap also performed well in other trap comparison studies, which were carried out in Spain  and Italy , although for other mosquito species. Roiz et al. , reported that the BG trap plus BG-Lure was more effective at capturing An. atroparvus host-seeking and blood-fed females with or without CO2 than the CDC trap plus CO2. In the study conducted in Italy , four traps were tested, including the CDC trap with CO2 and the Biogents BG Eisenhans de Luxe trap (basically the same as BG + lure + CO2, but where CO2 release is regulated by a computer); similar to our present study, the latter was the device that caught the most An. maculipennis s.l. However, contrasting results were described in other studies, where the CDC trap with CO2 collected more An. maculipennis s.l. than the BG trap with BG-Lure and CO2, but the small number of specimens captured do not allow us to draw detailed conclusions [42, 43].
A new trap has been recently designed for the collection of Anopheles: the BG-Malaria trap . It is very similar to BG + lure + CO2 used here, with minor modifications (it is an upside-down BG trap, thus the airflow is inverted), and its efficacy for sampling Anopheles species has been demonstrated in studies carried out in Brazil (Porto Velho, North Region)  and Africa (southeastern Tanzania) . It is considered the most effective trap for Anopheles collection in endemic areas [21, 44]. Our results are consistent with those reported for traps used in these and other studies, although they were performed in areas where other malaria vector species occur [24, 45].
In our study, the CDC trap used with light and baited with lure and CO2 did not perform significantly better (P = 0.321) than the CDC trap baited with only CO2. This result confirmed similar outcomes in other studies showing that a source of CO2 is essential to increase the number of host-seeking mosquitoes caught [40, 46,47,48], while light and lure had no significant effect on trap efficacy .
Of particular interest is evaluating the performance of the CDC trap plus CO2 and BG-Lure, as this is the most common combination of trap and attractants used in Europe for mosquito monitoring [19, 42]. The pairwise comparisons showed that CDC + CO2 was superior to BG + lure (P = 0.038) in collecting An. messeae/An. daciae and mosquitoes of other species, and thus should be the preferred method for monitoring Anopheles species, although the CDC-like trap used here damaged some of the captured specimens. The key factor for better trap performance is the use of CO2; however, the use of CO2 can be a limitation due to difficulty in its supply, its cost, or the preparation of a yeast mixture . Thus, a cost analysis should be done in advance regarding trap purchase, operation, and servicing to provide an indication of costs before mosquito monitoring is implemented. In our study, the purchase cost of the different traps was about the same (approximately 180 €); therefore, cost should not affect choice for either of these traps.
Manual aspiration was the most effective method for the capture of a large number of An. messeae/An. daciae and An. maculipennis s.s. in a short period of time during indoor collection from stables and dwellings, where these species rest. At both of the study sites, a single aspiration caught far more specimens of An. maculipennis s.l. (955 at site 1 and 104 at site 2) than the best-performing trapping device. However, manual aspiration is laborious and can mostly be performed only inside dwellings or shelters.
Although our results on trap performance primarily pertain to Anopheles messeae/Anopheles daciae and Anopheles maculipennis s.s., and are relevant to plans for further sampling of these species, they can also be used for the same purposes for other zoophilic species with similar behavior, such as Anopheles atroparvus and Anopheles superpictus , for which BG + lure + CO2 and CDC + CO2 are effective sampling tools. For species that are more anthropophilic, such as Anopheles labranchiae and Anopheles sacharovi, HLC remains the best method for evaluating Anopheles species occurrence and density, and provides additional useful information such as human biting rate [28, 51]. On the other hand, both HLC and traps baited with CO2 have been used successfully to collect An. labranchiae [6, 52].
A lower daily mean of Anopheles species captured by all the tested traps was observed at site 1, likely because of the presence of farm animals, which may have attracted more host-seeking mosquitoes than the traps. The influence of animal presence on catch effectiveness was observed in a previous study . The difference in mosquito collection rate between the sampling sites in the present study suggests confounding factors introduced by the occurrence of competing hosts at site 1 and a subsequent reduction of their attraction to the CO2-baited traps. We suggest that, to perform more efficient Anopheline surveillance, sites should be selected that are more distant from potential hosts than those used in the present study.
Anopheles species were last monitored in northeast Italy in the 1990s, when Anopheles maculipennis s.s. was the most abundant species on the Adriatic coast and Po river delta followed by An. atroparvus and An. messeae, while An. labranchiae was not found . Our results indicate that the species composition and distribution at the same monitoring sites have changed in the last 20 years, as An. atroparvus was not recorded in the present study, whereas An. messeae/An. daciae was the most abundant species at both sites and An. maculipennis s.s. was not common.
Anopheles maculipennis s.l. had the third highest occurrence at both sites (6.8%) after Cx. pipiens (62.9%) and Oc. caspius (27.7%). These three species represent 97.4% of all the mosquitoes collected during the study period. In general, both traps performed well in collecting these common mosquito species, but differed in their effectiveness for other less-represented species. Both the BG and CDC traps were effective in collecting Cx. pipiens and Oc. caspius when CO2 was added; the addition of other attractants (lure or light) only increased the number of Oc. caspius collected, which confirms previous observations [12, 27, 53]. For Aedes albopictus the BG trap has been confirmed to be the best, regardless of attractant used [27, 55, 56].
The data presented here provide researchers and field workers evidence that the tested traps, combined with certain attractants, can be used as sampling tools for An. messeae/An. daciae. Future work should assess the efficacy of these different trapping methods for the estimation of the abundance of the several Anopheles malaria vectors that occur in Europe, and the related risk of malaria transmission there.
Among the trapping methods discussed in the literature for the collection of malaria vectors, most have been developed to collect host-seeking Anopheles females in areas with circulating malaria, whereas few studies have tested traps for potential malaria vectors in Europe. To instigate appropriate surveillance strategies to evaluate in detail the distribution and abundance of Anopheles species, it is necessary to identify which trap is the most suitable for their sampling. Our results show that, among the combinations of traps and attractants tested, the BG-Sentinel trap baited with BG-Lure and CO2 is the best for routine monitoring of host-seeking Anopheles mosquito species such as An. messeae/An. daciae. The data presented here, despite being limited to a single species of the An. maculipennis complex, are of interest as they indicate a standardized and useful sampling approach that needs to be further investigated for its potential to sample other potential malaria vectors in Europe. As seen in studies on other major mosquito pests, when monitoring Anopheles species in Europe, it is also important to consider the following: host-seeking behavior and how anthropophilic the species is, a trap’s attractiveness to the mosquitoes in relation to the natural environment, host abundance, and also cost effectiveness. This information would fill a gap in knowledge for the monitoring of potential European malaria vectors of the genus Anopheles, of which An. messeae/An. daciae is one of the most widely distributed species . The possible reintroduction into Europe of Plasmodium parasites from endemic countries requires standardized sampling tools that allow researchers to overcome the limits to reproducibility associated with traditional approaches, such as HLC and manual aspiration. The identification of effective trapping devices for the consistent monitoring of Anopheles species in Europe is of major importance for malaria surveillance.
Availability of data and materials
Data supporting the conclusions of this article are included within the article and its additional files. The dataset and R script generated during the study are available from the corresponding author on reasonable request.
- BG trap:
BG-Sentinel 2 trap
- CDC trap:
Centers for Disease Control and Prevention-like trap
Total daily precipitation
Mean daily temperature
Countries and territories certified malaria-free by WHO. https://www.who.int/teams/global-malaria-programme/elimination/countries-and-territories-certified-malaria-free-by-who. Accessed 10 Jan 2022.
Sinka ME, Bangs MJ, Manguin S, Coetzee M, Mbogo CM, Hemingway J, et al. The dominant Anopheles vectors of human malaria in Africa, Europe and the Middle East: occurrence data, distribution maps and bionomic précis. Parasit Vectors. 2010;3:117.
Hertig E. Distribution of Anopheles vectors and potential malaria transmission stability in Europe and the Mediterranean area under future climate change. Parasit Vectors. 2019;12:18.
European Centre for Disease Prevention and Control. Malaria. In: ECDC. Annual epidemiological report for 2019. Stockholm (Sweden): ECDC; 2021.
Kuhn KG, Campbell-Lendrum DH, Davies CR. A continental risk map for malaria mosquito (Diptera: Culicidae) vectors in Europe. J Med Entomol. 2002;39:621–30.
Di Luca M, Boccolini D, Severini F, Toma L, Barbieri FM, Massa A, et al. A 2-year entomological study of potential malaria vectors in central Italy. Vector Borne Zoonotic Dis. 2009;9:703–11.
Brugueras S, Fernández-Martínez B, Martínez-de la Puente J, Figuerola J, Porro TM, Rius C, et al. Environmental drivers, climate change and emergent diseases transmitted by mosquitoes and their vectors in southern Europe: a systematic review. Environ Res. 2020;191: 110038.
Romi R, Boccolini D, Vallorani R, Severini F, Toma L, Cocchi M, et al. Assessment of the risk of malaria re-introduction in the Maremma plain (central Italy) using a multi-factorial approach. Malar J. 2012;11:98.
Boccolini D, Menegon M, Di Luca M, Toma L, Severini F, Marucci G, et al. Non-imported malaria in Italy: paradigmatic approaches and public health implications following an unusual cluster of cases in 2017. BMC Public Health. 2020;20:857.
Calzolari M, Desiato R, Albieri A, Bellavia V, Bertola M, Bonilauri, et al. Mosquitoes of the maculipennis complex in northern Italy. Sci Rep. 2021;11:6421.
Fornasiero D, Mazzucato M, Barbujani M, Montarsi F, Capelli G, Mulatti P. Inter-annual variability of the effects of intrinsic and extrinsic drivers affecting West Nile virus vector Culex pipiens population dynamics in northeastern Italy. Parasit Vectors. 2020;13:271.
European Centre for Disease Prevention and Control; European Food Safety Authority. Field sampling methods for mosquitoes, sandflies, biting midges and ticks—VectorNet project 2014–2018. Stockholm (Sweden) and Parma (Italy): ECDC and EFSA; 2018.
Service MW. A critical review of procedures for sampling populations of adult mosquitoes. Bull Entomol Res. 1977;67:343–82.
Silver JB. Mosquito ecology—field sampling methods. 3rd ed. Dordrecht: Springer; 2008. p. 1519.
European Centre for Disease Prevention and Control. Guidelines for the surveillance of native mosquitoes in Europe. Stockholm, Sweden: ECDC; 2014.
World Health Organization. Manual on practical entomology in malaria. Part I. Methods and techniques. Geneva: WHO; 1975.
World Health Organization. Manual on practical entomology in malaria. Part II. Methods and techniques. Geneva: WHO; 1975.
World Health Organization. Malaria entomology and vector control. Geneva: WHO; 2013.
Tangena JA, Hiscox A, Brey PT. Sampling adult populations of Anopheles mosquitoes. Methods Mol Biol. 2019;2013:233–85.
Lima JB, Rosa-Freitas MG, Rodovalho CM, Santos F, Lourenço-de-Oliveira R. Is there an efficient trap or collection method for sampling Anopheles darlingi and other malaria vectors that can describe the essential parameters affecting transmission dynamics as effectively as human landing catches? A review. Mem Inst Oswaldo Cruz. 2014;109:685–705.
Gama RA, Silva IM, Geier M, Eiras AE. Development of the BG-Malaria trap as an alternative to human-landing catches for the capture of Anopheles darlingi. Mem Inst Oswaldo Cruz. 2013;108:763–71.
Kilama M, Smith DL, Hutchinson R, Kigozi R, Yeka A, Lavoy G, et al. Estimating the annual entomological inoculation rate for Plasmodium falciparum transmitted by Anopheles gambiae s.l. using three sampling methods in three sites in Uganda. Malar J. 2014;13:111.
Maliti DV, Govella NJ, Killeen GF, Mirzai N, Johnson PCD, Kreppel K, Ferguson HM. Development and evaluation of mosquito-electrocuting traps as alternatives to the human landing catch technique for sampling host-seeking malaria vectors. Malar J. 2015;14:502.
Pombi M, Guelbeogo WM, Calzetta M, Sagnon N, Petrarca V, La Gioia V, et al. Evaluation of a protocol for remote identification of mosquito vector species reveals BG-Sentinel trap as an efficient tool for Anopheles gambiae outdoor collection in Burkina Faso. Malar J. 2015;14:161.
Sanou A, Moussa Guelbéogo W, Nelli L, Toé KH, Zongo S, Ouédraogo P, et al. Evaluation of mosquito electrocuting traps as a safe alternative to the human landing catch for measuring human exposure to malaria vectors in Burkina Faso. Malar J. 2019;18:386.
Ngom EHM, Virgillito C, Manica M, Rosà R, Pichler V, Sarleti N, et al. Entomological survey confirms changes in mosquito composition and abundance in Senegal and reveals discrepancies among results by different host-seeking female traps. Insects. 2021;12:692.
Drago A, Marini F, Caputo B, della ColuzziTorre MA, Pombi M. Looking for the gold standard: assessment of the effectiveness of four traps for monitoring mosquitoes in Italy. J Vector Ecol. 2012;37:117–23.
L’Ambert G, Ferré JB, Schaffner F, Fontenille D. Comparison of different trapping methods for surveillance of mosquito vectors of West Nile virus in Rhône Delta, France. J Vector Ecol. 2012;37:269–75.
Severini F, Toma L, Di Luca M, Romi R. Le Zanzare Italiane: generalità e identificazione degli adulti (Diptera, Culicidae). Fragm Entomol. 2009;41:213–372.
Lühken R, Czajka C, Steinke S, Jöst H, Schmidt-Chanasit J, Pfitzner W, et al. Distribution of individual members of the mosquito Anopheles maculipennis complex in Germany identified by newly developed real-time PCR assays. Med Vet Entomol. 2016;30:144–54.
Nicolescu G, Linton YM, Vladimirescu A, Howard TM, Harbach RE. Mosquitoes of the Anopheles maculipennis group (Diptera: Culicidae) in Romania, with the discovery and formal recognition of a new species based on molecular and morphological evidence. Bull Entomol Res. 2004;94:525–35.
Naumenko AN, Karagodin DA, Yurchenko AA, Moskaev AV, Martin OI, Baricheva EM, et al. Chromosome and genome divergence between the cryptic Eurasian malaria vector-species Anopheles messeae and Anopheles daciae. Genes. 2020;11:165.
ARPA Veneto, Dati ambientali. 2019. http://www.arpa.veneto.it. Accessed 10 Jan 2022.
Beaujean AA, Grant MB. Tutorial on using regression models with count outcomes using R. Pract Assess Res Eval. 2016;21:1–19.
Venables WN, Ripley BD. Modern applied statistics with S. 4th ed. New York: Springer; 2002.
R Core Team. R: a language and environment for statistical computing. R Foundation for Statistical Computing. Vienna, Austria; 2021. https://www.R-project.org/. Accessed 18 Mar 2022.
Wickham H. ggplot2: elegant graphics for data analysis. New York: Springer; 2016.
Lenth RV. emmeans: estimated marginal means, aka least-squares means. R package version 1.4.7. 2020. https://CRAN.R-project.org/package=emmeans. Accessed 18 Mar 2022.
Hartig F. DHARMa: residual diagnostics for hierarchical (multi-level/mixed) regression models. R package version 0.4.5. 2022. https://CRAN.R-project.org/package=DHARMa. Accessed 18 Mar 2022.
Pombi M, Jacobs F, Verhulst NO, Caputo B, della Torre A, Takken W. Field evaluation of a novel synthetic odour blend and of the synergistic role of carbon dioxide for sampling host-seeking Aedes albopictus adults in Rome, Italy. Parasit Vectors. 2014;7:580.
Roiz D, Roussel M, Muñoz J, Ruiz S, Soriguer R, Figuerola J. Efficacy of mosquito traps for collecting potential West Nile mosquito vectors in a natural Mediterranean wetland. Am J Trop Med Hyg. 2012;86:642–8.
Lühken R, Pfitzner WP, Börstler J, Garms R, Huber K, Schork N, et al. Field evaluation of four widely used mosquito traps in Central Europe. Parasit Vectors. 2014;7:268.
Hoshi T, Brugman VA, Sato S, Ant T, Tojo B, Masuda G, et al. Field testing of a lightweight, inexpensive, and customisable 3D-printed mosquito light trap in the UK. Sci Rep. 2019;9:11412.
Batista EPA, Ngowo HS, Opiyo M, Shubis GK, Meza FC, Okumu FO, et al. Semi-field assessment of the BG-Malaria trap for monitoring the African malaria vector, Anopheles arabiensis. PLoS ONE. 2017;12: e0186696.
Ponlawat A, Khongtak P, Jaichapor B, Pongsiri A, Evans BP. Field evaluation of two commercial mosquito traps baited with different attractants and colored lights for malaria vector surveillance in Thailand. Parasit Vectors. 2017;10:378.
Hoel DF, Dunford JC, Kline DL, Irish SR, Weber M, Richardson AG, et al. A comparison of carbon dioxide sources for mosquito capture in Centers for Disease control and Prevention light traps on the Florida Gulf coast 1. J Am Mosq Control Assoc. 2015;31:248–57.
Van Loon JJ, Smallegange RC, Bukovinszkiné-Kiss G, Jacobs F, De Rijk M, Mukabana WR, et al. Mosquito attraction: crucial role of carbon dioxide in formulation of a five-component blend of human-derived volatiles. J Chem Ecol. 2015;41:567–73.
Van de Straat B, Hiscox A, Takken W, Burkot TR. Evaluating synthetic odours and trap designs for monitoring Anopheles farauti in Queensland. Australia Malar J. 2019;18:299.
Becker N, Zgomba M, Petric D, Ludwig M. Comparison of carbon dioxide, octenol and a host-odour as mosquito attractants in the Upper Rhine Valley, Germany. Med Vet Entomol. 1995;9:377–80.
Aldridge RL, Britch SC, Allan SA, Tsikolia M, Calix LC, Bernier UR, et al. Comparison of volatiles and mosquito capture efficacy for three carbohydrate sources in a yeast-fermentation CO2 generator. J Am Mosq Control Assoc. 2016;32:282–91.
Toty C, Barré H, Le Goff G, Larget-Thiéry I, Rahola N, Couret D, et al. Malaria risk in Corsica, former hot spot of malaria in France. Malar J. 2010;9:231.
Danis K, Baka A, Lenglet A, Van Bortel W, Terzaki I, Tseroni M, et al. Autochthonous Plasmodium vivax malaria in Greece, 2011. Euro Surveill. 2011;16:19993.
Kline DL. Traps and trapping techniques for adult mosquito control. J Am Mosq Control Assoc. 2006;22:490–6.
Zamburlini R, Cargnus E. Anofelismo residuo nel litorale altoadriatico a 50 anni dalla scomparsa della malaria. Parassitologia. 1998;40:431–7.
Krockel U, Rose A, Eiras AE, Geier M. New tools for surveillance of adult yellow fever mosquitoes: comparison of trap catches with human landing rates in an urban environment. J Am Mosq Control Assoc. 2006;22:229–38.
Gibson-Corrado J, Smith ML, Xue RD, Meng FX. Comparison of two new traps to the Biogents BG-Sentinel trap for collecting Aedes albopictus In North Florida. J Am Mosq Control Assoc. 2017;33:71–4.
Bertola M, Mazzucato M, Pombi M, Montarsi F. Updated occurrence and bionomics of potential malaria vectors in Europe: a systematic review (2000–2021). Parasit Vectors. 2022;15:88.
We wish to thank Dr. Paolo Mulatti (Istituto Zooprofilattico Sperimentale Delle Venezie; IZSVe) for his useful insights regarding the data analysis and Giulio Marchetti (IZSVe) for drawing the maps. We thank Prof. Alessandra della Torre and Dr. Beniamino Caputo (Sapienza University of Rome) for their suggestions regarding the design of the study. We wish also to thank Riccardo Piva, Marco Mazzaro, Andrea Longo, Federico Tomasello, from the environmental entomology course at the University of Padua, for their involvement in the mosquito trapping activities.
The study was financially supported by the Integrated Surveillance and Vector-borne Disease Control 2018 Veneto Regional Prevention Plan (Regional Committee resolution no. 184440 on 28 May 2018).
Ethics approval and consent to participate
Consent for publication
The authors declare that they have no competing interests. PeP (manufacturer of the CDC-like trap) and Biogents (manufacturer of the BG-Sentinel trap) had no role in the design of this study, in the provision of materials, or in the preparation of the manuscript.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Sampling sites and climatic data (temperature and precipitation) for the sampling period (trapping cycle). Table S2. Numbers and percentages of adults of species of the Anopheles maculipennis complex molecularly identified, according to collection method.
DHARMa residual diagnostics plots  (qq-plot and residuals plotted against the predicted value) for the Poisson regression model (upper plots) and the negative binomial regression model (lower plots) for Anopheles messeae/Anopheles daciae. Tests for correct distribution (KS test), dispersion and outliers are shown on the plots.
Shapiro–Wilk test and Levene’s test to check requirements of normality and homogeneity of variance across groups, respectively.
. Incident rate ratios (i.e. exponential of coefficient estimates), 95% CIs and significance for Culex pipiens and Ochlerotatus caspius. Table S5. Contrasts between trapping devices for estimated marginal means after model fitting, considering a confidence level of 0.95, for Culex pipiens and Ochlerotatus caspius. Estimates are back-transformed from the log scale.
About this article
Cite this article
Bertola, M., Fornasiero, D., Sgubin, S. et al. Comparative efficacy of BG-Sentinel 2 and CDC-like mosquito traps for monitoring potential malaria vectors in Europe. Parasites Vectors 15, 160 (2022). https://doi.org/10.1186/s13071-022-05285-9