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Identification and evaluation of UL36 protein from Dermacentor silvarum salivary gland and its interaction with Anaplasma ovis VirB10



Anaplasma ovis is a gram-negative, tick-borne obligate intraerythrocytic pathogen, which causes ovine anaplasmosis in small ruminants worldwide. VirB10 of A. ovis is an integral component of the Type IV Secretion System (T4SS). The T4SS is used by bacteria to transfer DNA and/or proteins undeviatingly into the host cell to increase their virulence. To more thoroughly understand the interaction between A. ovis and Dermacentor silvarum, a vector containing the virb10 gene of A. ovis was used as a bait plasmid to screen interacting proteins from the cDNA library of the D. silvarum salivary gland using the yeast two-hybrid system.


The cDNA of the D. silvarum salivary gland was cloned into the pGADT7-SmaI vector (prey plasmid) to construct the yeast two-hybrid cDNA library. The virb10 gene was cloned into the pGBKT7 vector to generate a bait plasmid. Any gene auto-activation or toxicity effects in the yeast strain Y2HGold were excluded. The screening was performed by combining the bait and prey plasmids in yeast strains to identify positive preys. The positive preys were then sequenced, and the obtained sequences were subjected to further analyses using Gene Ontology, UniProt, SMART, and STRING. Additionally, the interaction between the bait and the prey was evaluated using the glutathione S-transferase (GST) pull-down assay.


A total of two clones were obtained from the cDNA library using the yeast two-hybrid system, and the sequence analysis showed that both clones encoded the same large tegument protein, UL36. Furthermore, the proteins GST-UL36 and His-VirB10 were successfully expressed in vitro and the interaction between the two proteins was successfully demonstrated by the GST pull-down assay.


To our knowledge, this study is the first to screen for D. silvarum salivary gland proteins that interact with A. ovis VirB10. The resulting candidate, UL36, is a multi-functional protein. Further investigations into the functionality of UL36 should be carried out, which might help in identifying novel prevention and treatment strategies for A. ovis infection. The present study provides a base for exploring and further understanding the interactions between A. ovis and D. silvarum.


Anaplasma ovis is a gram-negative, tick-borne obligate intraerythrocytic pathogen [1], which causes ovine anaplasmosis in small ruminants worldwide [2]. The symptoms of the disease include severe anemia, fever, weight loss, spontaneous abortion, jaundice, and even death, in sheep and goats [3]. The economic impact of A. ovis is becoming increasingly serious in countries where sheep and goats are the most important livestock [4]. The transmission of this pathogen is primarily via tick bites [5]; the tick vector species are mainly species of the genera Dermacentor, Rhipicephalus and Hyalomma and play a crucial role in maintaining and spreading the pathogen [5,6,7]. As yet, the vertical transmission of A. ovis in ticks has not been substantiated. It is known that A. ovis initially infects the midguts of ticks, and then migrates to the salivary glands where the pathogens are transmitted to the new hosts during blood-feeding [8, 9].

Bacteria use the Type IV Secretion System (T4SS) to undeviatingly transfer virulence, DNAs and/or proteins, into the host cell [10]. The T4SS is a 1.1 MDa multi-protein complex, composed of 12 proteins (including VirB1-11 and VirD4), and this protein complex resides within the membrane bilayers of bacteria [11]. The core complex of T4SS comprisesVirB10, in conjunction with other proteins [12]; therefore, VirB10 is an integral component of the system [12]. The core complex is embedded in the outer and inner membranes and plays an active role in the transfer of the Type IV system’s substrate [13].

A previous study has shown that VirB10 is homologous to TrbI and vir gene proteins, which mediate conjugation-like processes [14]. Comparative studies of the T4SS of unrelated bacteria revealed that the VirB7-VirB11 proteins, including VirB10, are the basic component of the T4SS [15]. A study of the Agrobacterium tumefaciens secretion system found that VirB8-VirB10 interact with each other and play protein initiator roles during the assembly process [16, 17]. VirB10 is an essential component of a trans-membrane pathway for the exportation, biosynthesis and transmission of DNA. The T4SS of obligate intracellular pathogens have a preeminent role in survival, nutrition and virulence [18]. It has been demonstrated that the T4SS also functions in the tick-infection stage of Anaplasma and Ehrlichia life-cycles [19, 20]. As ascertained previously, T4SS is associated with a bacterial conjugal system that transports effector macromolecules produced by bacteria into eukaryotic target cells [21]. The regulation and expression of T4SS protein genes, including virb10, were investigated, and replication, cellular transportation, and intracellular survival were explored as potential functions of T4SS in rickettsial pathogens [22].

There have been previous studies involving the screening and identification of possible host or proteins of bacterial origin that interact with Anaplasma spp. (other than A. ovis), but few interacting proteins from ticks have been reported. In this study, to more thoroughly understand the interaction between A. ovis and D. silvarum, a recombinant virb10 gene was used as bait to screen potential interacting proteins from the cDNA library of the D. silvarum salivary gland using a yeast two-hybrid system.


Isolation of the tick salivary glands

To collect salivary glands, A. ovis infection-free adult ticks (D. silvarum) were used. The ticks were maintained for several consecutive generations at the State Key Laboratory of Veterinary Etiological Biology of Lanzhou Veterinary Research Institute, China, under optimum temperature and humidity. Ticks were tested for Anaplasma infection by PCR using the primer pairs EE1/E2, AB1f/1r, SSAP2f/2r, AmargMSP4Fw/4Rev and MSP45/43 [23,24,25,26]. To collect the salivary glands, each tick was dissected into two parts between coxa 1 and coxa 2 using a microtome. Then, the anterior part was removed from the posterior using sterile tweezers. The salivary glands were carefully detached from the anterior part without damaging the midgut. The salivary glands were then collected into a sterile 1.5 ml tube containing 500 μl Trizol (Sigma-Aldrich, Cleveland, OH, USA). All dissection steps were performed with great care under a stereomicroscope to avoid contamination by the midgut fluid.

Construction of a yeast two-hybrid cDNA library of salivary glands

The salivary glands collected from D. silvarum were sent to Takara (Dalian, China) for the construction of a yeast two-hybrid cDNA library. The total RNA of the salivary glands was extracted and reverse transcribed into first-strand cDNA. Following normalization treatment using a cDNA Normalization Kit (Invitrogen, Carlsbad, CA, USA) and short fragment removal using Make Your Own “Mate & Plate™” Library System (Clontech, Mountain View, CA, USA), the salivary gland cDNA was cloned into the pGADT7-SmaI vector (prey plasmid) for the construction of the cDNA library.

Bait plasmid construction

For the construction of the bait plasmid (A. ovis virb10), the genomic DNA was extracted from 200 μl A. ovis (Heibei strain)-infected whole blood using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. The virb10 gene fragment was amplified by PCR from the genomic DNA, based on the specific primers VB10otF/R (Table 1). Extracted DNA (2 μl) was used as the template in a 25 μl volume PCR reaction mixture, comprising 12.5 μl Takara PCR Master Mix, 1 μl forward and reverse primers, and nuclease-free water. PCR conditions consisted of initialization at 94 °C for 4 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 58 °C for 30 s, extension at 72 °C for 1 min 30 s, and a final extension at 72 °C for 15 min. The amplified fragment after purification and the empty pGBKT7 plasmid were both digested with the restriction enzymes NdeI and SalI (NEB, Beverly, MA, USA). The total reaction mixture was 50 μl and contained 1 μg insert/plasmid, 1 μl restriction enzyme, and 5 μl 10× NEBuffer in nuclease-free water. The mixtures were incubated at 37 °C for 1 h to allow the reaction to complete. The incised insert and plasmid (pGBKT7) were purified and then ligated at 16 °C overnight with T4 DNA ligase. The recombinant pGBKT7-VirB10 plasmid was confirmed by PCR amplification and sequencing (Sangon Biotech, Shanghai, China).

Table 1 Primers and PCR amplification conditions

Auto-activation and toxicity tests

The bait protein was tested for auto-activation and toxicity before the Y2H screening. Following the manufacture’s protocols for the Yeastmaker™ Yeast Transformation System 2 (Clontech, Mountain View, CA, USA), the pGBKT7-VirB10, pGBKT7, pGBKT7-53 positive control and pGBKT7-Lam negative control plasmids were transformed into Y2HGold. Transformants were grown on SD/-Trp, SD/-Trp/X (40 μg/ml X-α-Gal), SD/-Trp/X /A (40 μg/ml X-α-Gal and 125 ng/ml Aureobasidin A) or SD/-Leu/-Trp/ X-αGal/AbA (40 μg/ml X-α-Gal and 125 ng/ml Aureobasidin A) agar plates at 30 °C for 3–5 days. Only the bait plasmids that did not show auto-activation and toxicity were used in yeast two-hybrid screening.

Mate & Plate Library screening

To screen the cDNA library for identifying potential interacting tick salivary gland proteins, the Matchmaker™ Gold Yeast Two-Hybrid System (Clontech) was used, according to the manufacturer’s protocols. Briefly, a concentrated cultured bait strain (pGBKT7-VirB10) and thawed library yeast strain (prey) were prepared and mated. The blended mixture was cultured in 2× YPDA/Kan medium and incubated at 30 °C with slow shaking (30–50× rpm) for 20–24 h until a typical three-lobed zygote could be seen under the microscope. The mated culture was coated on SD/-Trp, SD/-Leu and SD/-Trp/Leu medium for 3–5 days at 30 °C to determine the mating efficiency, and the remainder of the culture was grown on DDO/X/A (SD/-Trp/-Leu/X-αGal/Aba) medium for 3–5 days at 30 °C. All the blue colonies that grew on DDO/X/A were transferred onto higher stringency SD/-Ade/-His/-Leu/-Trp/X-α-Gal/AbA (QDO/X/A) agar plates for 3–5 days at 30 °C using a flat sterile toothpick.

Selection of the positive prey plasmids

To confirm the interactions, the prey plasmids were extracted from putative positive clones using the Easy Yeast Plasmid Isolation Kit (Cat. No. 630467; Clontech). Subsequently, each prey plasmid was transformed into Escherichia coli DH5α competent cells (Transgen, Beijing, China). For transformation, 100 μl E. coli DH5α competent cells were gently mixed with extracted prey plasmids and kept in an ice bath for 30 min. Cells were then incubated at 42 °C for 45 s and returned to the ice bath for 2 min. SOC medium (500 μl) was pre-incubated at 37 °C and added to the cells, which were incubated by shaking (220× rpm) for 2 h at 37 °C. After plating 100 μl of culture onto LB/Amp agar plates and incubating overnight at 37 °C, the transformants growing on the LB/Amp agar plates were purified using the Plasmid Mini Kit I (Cat. No. D6943-02; Omega, Doraville, GA, USA). Following this, each putative positive prey plasmid from the initial screening was co-transformed into Y2HGold strain with the bait plasmid. Transformants were then spread onto QDO/X/A plates to test for interactions. Positive hits appeared as blue colonies under these conditions. To estimate each insert size of the potential positive prey plasmids, primers pGADT7-F (5′-TAA TAC GAC TCA CTA TAG GGC-3′) and pGADT7-R (5′-AGA TGG TGC ACG ATG CAC AG-3′), were used for PCR amplification.

Cloning and expression of VirB10

To further validate the interactions between VirB10 and prey protein, the coding sequences of bait and prey plasmids were amplified and cloned into pET30a and pGEX-4T-1 plasmids, respectively. The coding sequence of VirB10 was amplified using primers V10-32F/R (Table 1) and cloned into a pGEM-T Easy Vector (Promega, Beijing, China). The recombinant plasmid pGEMT-VirB10 was digested with BamHI and NotI (NEB). The total 50 μl reaction mixture contained 1 μg insert/plasmid, 1 μl restriction enzyme, 5 μl 10× NEBuffer, and nuclease-free water. The mixtures were incubated at 37 °C for 1 h to fully react. After purification, the incised insert and plasmid were ligated at 16 °C overnight with T4 DNA ligase. Similarly, the coding sequence of the prey protein (UL36) was amplified using primers S41F/R (Table 1) and cloned into the pGEM-T Easy Vector. The recombinant plasmid pGEMT-UL36 was digested with BamHI and NotI and sub-cloned into pGEX4T-1 as described above. The recombinant His-bait plasmid was transformed and expressed in the E. coli strain BL21DE3 while the GST-prey plasmid was transformed and expressed in the E. coli strain BL21 (TransGen, Beijing, China). The final obtained clones were then inoculated in LB broth supplemented with kanamycin and cultured overnight. This overnight culture was used for further inoculation of fresh LB broth, and expression was induced with 1mM isopropyl-β-D thiogalactopyranoside (IPTG) at 0.6 OD. The expression of His-VirB10 and GST-UL36 was determined by western blot analysis.

Purification of VirB10

The recombinant protein was purified under denaturing conditions. Briefly, the 50 ml induced bacterial culture was pelleted after centrifugation at 4000× g for 20 min and washed twice with 1× phosphate-buffered saline (PBS). The washed pellet was dissolved in 8 ml lysis buffer (8 M urea, 100 mM NaH2PO4, 10 mM Tris-Cl, 20 mM β-mercaptoethanol, 1% Triton X-100, 1 mM phenylmethylsulfonyl fluoride) at pH 8.0, sonicated for 12 min, and incubated for 2 h with continuous stirring at 175× rpm at room temperature. After incubation, the lysed cell suspension was centrifuged at 12,000× rpm for 25 min at room temperature, and the supernatant was collected and mixed with Ni-NTA agarose (Qiagen) at a 1:3 ratio (super-flow Ni-NTA slurry: lysate) for 1 h to allow the recombinant VirB10 His-tagged protein to bind with Ni2+ in the super-flow slurry. The agarose super-flow Ni-NTA slurry-lysate mix was then packed into a 15 ml column and flow-through was collected. The column was then washed with 20 ml wash buffer (8 M urea, 100 mM NaH2PO4, 10mM Tris-Cl, 1% Triton X-100, 10% glycerol, pH 6.3) to remove the unbound or weakly-bound non-specific proteins. The recombinant VirB10 protein was then eluted from the column using 1 ml elution buffer (8 M urea, 100 Mm NaH2PO4, 10 mM Tris-Cl, pH 4.5) for 5 fractions and further analyzed on 12% SDS-PAGE. Refolding or dialysis of the purified protein was performed with a decreasing urea gradient (8 M, 6 M, 4 M, 2 M and 1 M) and finally against 1× PBS. The concentration of purified recombinant protein was estimated by the Bradford method using bovine serum albumin (BSA) as standard [27].

Glutathione S-transferase (GST) pull-down

Following protocols of the PierceTM GST Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, Waltham, MA, USA), we equilibrated glutathione agarose. Briefly, the GST-UL36 protein was immobilized on the Pierce spin column. The His-VirB10 protein was captured by the Pierce spin column containing the immobilized GST-tagged prey protein. Then the bait-prey protein was eluted with glutathione elution buffer. To confirm that the His-VirB10 and GST-UL36 recombinant proteins did not bind to the GST and His tag, the GST-UL36 protein and GST tag were immobilized on the Pierce spin columns to capture His tag and His-VIRB10 proteins, respectively, and eluted by glutathione elution buffer. The protein elution was analyzed by western blotting with anti-His tag (1/2000, Sigma-Aldrich, Cleveland, OH, USA) or anti-GST tag mouse monoclonal antibodies (1/2000, Signalway Antibody, College Park, MD, USA). This experiment was repeated to confirm the results are credible.

Western blot

Equivalent amounts of eluted proteins were run on a 12% SDS-PAGE gel and then transferred to PVDF membranes (Millipore, Burlington, MA, USA) using a semi-dry Trans-Blot Turbo Transfer System (BioRad, Irvine CA, USA). The membrane was incubated with a blocking buffer (TBS containing 5% BSA and 0.05% Tween-20) for 1 h at room temperature. Incubations with the diluted primary antibody in TBS 0.05% Tween-20 were performed at 4 °C overnight. After 2 h incubation with an anti-rabbit or anti-mouse peroxidase-conjugated antibody (1/5000, Sigma-Aldrich) at room temperature, the membrane was washed five times (5 min each) with PBST. The membrane was then treated with ImmunoCruz™ Western Blotting Luminol Reagent (Santa Cruz Biotechnology, Heidelberg, Germany) according to the manufacturer’s instructions.

Sequence analysis

The sequences of the positive prey plasmids were analyzed and searched against the NCBI databases to analyze the function of the corresponding gene. The protein function of the identified gene was analyzed using Gene Ontology (, UniProt database (, SMART ( and STRING (


Construction of a yeast two-hybrid cDNA library

The quality of the cDNA library showed the cDNA library titer was 4.8 × 106 cfu (Fig. 1a), and the average size of inserted fragments was > 1000 bp, according to the PCR amplification results of 24 random clones (Fig. 1b). These results indicated that the cDNA library could be used for yeast two-hybrid screening.

Fig. 1
figure 1

a Clone counts. For the dilution factor 10−2, the number of clones on the plate was 1200 giving 4.8 × 106 cfu. b Determining prey inserts sizes. Agarose gel electrophoresis of amplified PCR products from 24 random clones of cDNA fragments from D. silvarum with average size > 1000 bp (Lanes 1–24)

Construction of bait plasmid and testing its auto-activation and toxicity

Sequence analysis of the cloned virb10 showed it encodes 441 amino acid residues (from aa 1 to aa 441) (Fig 2a). The recombinant plasmid (VirB10-pGBKT7) was confirmed by PCR amplification (Fig. 2b). The results of the bait plasmid auto-activation testing showed that the yeast transfected with a plasmid containing pGBKT7-VirB10 or empty pGBKT7 were white on SD/-Trp/X plates, and the positive control colonies were blue on DDO/X/A plates (Fig. 2c). This proved that the recombinant plasmid pGBKT7-VirB10 did not autonomously activate the reporter genes in Y2HGold in the absence of a prey protein. The colony size of Y2HGold transformed with bait plasmid was similar to that of Y2H Gold transformed with the pGBKT7. Based on these results, the pGBKT7-VirB10 bait plasmid was qualified for use in the yeast two-hybrid screening.

Fig. 2
figure 2

a Gene structure of the virb10 bait used in yeast-two-hybrid screening. TM, LC and UN stand for the trans-membrane region, low complexity region, and unknown region, respectively. b Construction of pGBKT7-VirB10 bait plasmid confirmed by agarose gel electrophoresis. Lane M: DL2000 DNA marker; Lane 1: confirmation of amplified bait (pGBKT7-VirB10) clone. c Determination of auto-activation and toxicity for pGBKT7-VirB10 bait plasmid in yeast cells. The pGBKT7-VirB10 bait and pGBKT7 plasmids were used to transform Y2HGold cells, which were grown on separate plates. The co-transformants containing pGADT7-T and pGBKT7-53 were grown on DDO/X/A plates as a positive control

Identification of positive prey from the cDNA library

After mating of bait-prey plasmids and achieving growth on the DDO/X/A plates, five blue clones were obtained (Fig. 3a). The five blue clones were subsequently re-plated twice onto higher stringency QDO/X/A plates. Two of the five colonies still manifested a blue color after two rounds on QDO/X/A, indicating that they were likely to be true positive hits (Fig. 3b). The prey plasmids were then isolated from their corresponding colonies and rescued through the transformation of E. coli DH5α cells. After PCR amplification using the primers pGADT7-F/R, the size of the inserted fragment in each prey plasmid was determined by gel electrophoresis (Fig. 3c). To eliminate false positive hits, both prey plasmids were mated with pGBKT7-VirB10 into Y2HGold cells and were cultivated on QDO/X/A plates, which showed blue colonies. Meanwhile, both rescued prey plasmids were mated with empty pGKBT7 plasmids into Y2HGold cells, without any growth on QDO/X/A plates (Fig. 3d).

Fig. 3
figure 3

Analysis of putative positive colonies. a Mated bait-prey plasmids (transformed in yeast) showing blue colonies on DDO/X/A. b Two of the five blue clones patched out onto higher stringency QDO/X/A plates manifested blue patches. c Agarose gel electrophoresis of amplified PCR products for the inserts of putative positive prey plasmids. Lane M: DNA 5000 maker; Lanes 1 and 2: PCR amplification products of the inserts of the two putatively positive hits. d Mated prey plasmid and pGBKT7-VirB10 in Y2HGold cells cultivated on QDO/X/A plates showed blue colonies while mated prey plasmid and empty pGKBT7 showed no growth on QDO/X/A plates

Sequence analysis of positive prey plasmid

To characterize the identified clones, both prey plasmids were sequenced using the primers pGADT7-F/R. The sequences were analyzed using the BLAST tool in NCBI. As a result, both plasmids showed 100% similarity with each other and 80% similarity with Ixodes scapularis conserved hypothetical protein-UL36 (XP_002436149.2). Gene ontology, UniProt, SMART, and STRING analyses showed the UL36 to be a large tegument protein with epidermal growth factor (EGF), which is involved in several cellular functions, including pleiotropic proliferation, developmental cell cycle control, and novel deubiquitinating activities [28,29,30,31].

In vitro evaluation of the interaction between UL36 and VirB10

The results of western blot analysis of the recombinant protein His-VirB10, which was transformed into E. coli BL21 (DE3) competent cells, showed there was successful expression of a 57.7 kD protein. GST-UL36 was transformed into competent cells BL21, which successfully expressed a 65.5 kD protein (Fig. 4a, b). The recombinant proteins GST-UL36 and His-VirB10 were used for the GST pull-down assay. Eventually, the anti-His tag (1/2000, Sigma-Aldrich) was used for the western blot, and a target band of about 123 kD was obtained, proving that there was genuine interaction between the two proteins. GST pull-down showed that the GST-UL36 protein did not bind to the His-tag protein. Meanwhile, the His-VirB10 protein also showed no binding to the GST-tag protein in vitro. In contrast, GST-UL36 bound to His-VirB10, indicating a direct interaction between VirB10 and UL36 (Fig. 4c).

Fig. 4
figure 4

Western blots of recombinant protein expression and GST pull-down results. a Expression of IPTG-induced recombinant protein His-VirB10 (approximately 57.7 kD). b Expression of IPTG-induced recombinant protein GST-UL36 (approximately 65.5 kD). c The input His-VirB10 and GST-tag mixture was eluded showing no binding between the His-VirB10 recombinant protein and GST-tag protein. d The fusion protein GST-UL36-His-VirB10 (approximately 123 kD) was obtained after GST pull-down assay using anti-His tag. e The input GST-UL36 and His-tag mixture was eluded showing no binding between the GST-UL36 recombinant protein and His-tag protein


The immunoelectron microscopy and genetic analysis established VirB10 as a basic structural component of the T4 secretory system [16, 17]. Bacteria utilize T4SS to transfer proteins and genetic material from the bacteria to host or vector cells [32]. VirB10 is a bacterial conjugation TrbI-like protein [14] that participates in secretion, intracellular trafficking and vesicular transportation [15]. The VirB10 protein in T4SS facilitates cellular transportation by constituting a membrane channel that is incorporated into inner and outer membranes [33].

In this study, the cDNA of the D. silvarum salivary gland was cloned into the pGADT7-SmaI vector, which had three different reading frames to ensure the correct expression of each protein. Following a high-throughput yeast two-hybrid screening, two putative positive clones were obtained. However, sequence analysis showed that both clones encoded the same protein (UL36), which was found to interact with VirB10 in screening experiments, suggesting that this assay had a low false-positive rate. Sequence analysis of VirB10 using the online software SMART demonstrated that it incorporates one transmembrane region (aa 28–46) and one Tbrl domain (aa 234–434). The full virb10 gene was cloned into the pGBKT7 vector to screen D. silvarum salivary gland interacting proteins using the Y2H system. Subsequently, a conserved hypothetical protein, UL36, from D. silvarum salivary glands was identified as interacting with VirB10. To the best of our knowledge, the present study is the first to discover that the conserved hypothetical protein, UL36, from the tick salivary gland interacts with A. ovis VirB10, using the Y2H and GST pull-down techniques.

UL36 has an EGF-like domain and is a large tegument protein [34, 35]. EGF is a short peptide that contains an idiosyncratic motif of six cysteines [34]. Homologous repeats of EGF catalyze proteolytic activation, which is integral to the regulation of extracellular proteolysis [36]. Various proteins with EGF domains and homologous sequences bolster the cell adhesion of normal and infected/tumor cells [37]. EGF is a protein of the coagulation and fibrinolytic pathway, suggesting these pathways are necessary for EGF interactions with receptor or ligand [31]. Several pathogenic microorganisms manipulate the ubiquitination status of host cells by both conjugation and deconjugation [38,39,40,41]. Extensive research into the Herpes virus UL36 has revealed that UL36 activity culminates at the late stages of herpes virus replication [42]. The significant cellular activities of the UL36 protein, ascertained by various studies, have included pleiotropic proliferation, developmental effects, adhesion of infected cells, and modification of ubiquitination. Although the existence of an interaction between VirB10 and UL36 is clear from the present study, the functional mechanisms involved require further investigation. Because of the peak in UL36 protein activity during the late stages of replication in the Herpes virus, we hypothesized that the VirB10-UL36 interaction plays an important role in bacteria’s virulence while in the tick salivary glands, as this is the site from where A. ovis is transferred to the host. Before entering the host, VirB10-UL36 might help to boost the number of infected cells; however, further functionality studies are needed to uncover the exact molecular mechanisms involved.


The interaction of the A. ovis VirB10 and tick salivary gland UL36 protein was corroborated by the GST pull-down assay, indicating that both proteins are involved in A. ovis development in D. silvarum. Further investigations to explore the functionality of UL36 in A. ovis and the role of interactions between A. ovis and D. silvarum are necessary.

Availability of data and materials

All data generated or analyzed during this study are included in this published article.



complementary deoxyribonucleic acid


isopropyl β-D-1-thiogalactopyranoside


phosphate-buffered saline


polymerase chain reaction


sodium dodecyl sulfate-polyacrylamide gel electrophoresis


tris-buffered saline


Type IV Secretion System


yeast two-hybrid


  1. Aubry P, Geale DW. A review of bovine anaplasmosis. Transbound Emerg Dis. 2011;58:1–30.

    Article  CAS  PubMed  Google Scholar 

  2. Kuttler KL. Anaplasma infections in wild and domestic ruminants: a review. J Wildl Dis. 1984;20:12–20.

    Article  CAS  PubMed  Google Scholar 

  3. Wei R, Liu HB, Jongejan F, Jiang BG, Chang QC, Fu X, et al. Cultivation of Anaplasma ovis in the HL-60 human promyelocytic leukemia cell line. Emerg Microbes Infect. 2017;6:e83.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Rymaszewska A, Grenda S. Bacteria of the genus Anaplasma—characteristics of Anaplasma and their vectors: a review. Vet Med. 2008;53:573–84.

    Article  Google Scholar 

  5. Friedhoff KT. Tick-borne diseases of sheep and goats caused by Babesia, Theileria or Anaplasma spp. Parassitologia. 1997;39:99–109.

    CAS  PubMed  Google Scholar 

  6. Parola P, Raoult D. Ticks and tickborne bacterial diseases in humans: an emerging infectious threat. Clin Infect Dis. 2001;32:897.

    Article  CAS  PubMed  Google Scholar 

  7. Yin H, Luo J. Ticks of small ruminants in China. Parasitol Res. 2007;101:187–9.

    Article  Google Scholar 

  8. Telford SR 3rd, Dawson JE, Katavolos P, Warner CK, Kolbert CP, Persing DH. Perpetuation of the agent of human granulocytic ehrlichiosis in a deer tick-rodent cycle. Proc Natl Acad Sci USA. 1996;93:6209–14.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Socolovschi C, Mediannikov O, Raoult D, Parola P. The relationship between spotted fever group rickettsiae and ixodid ticks. Vet Res. 2009;40:34.

    Article  PubMed  PubMed Central  Google Scholar 

  10. Chen L, Chen Y, Wood DW, Nester EW. A new type IV secretion system promotes conjugal transfer in Agrobacterium tumefaciens. J Bacteriol. 2002;184:4838–45.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Christie PJ, Atmakuri K, Krishnamoorthy V, Jakubowski S, Cascales E. Biogenesis, architecture, and function of bacterial type IV secretion systems. Annu Rev Microbiol. 2005;59:451–85.

    Article  CAS  PubMed  Google Scholar 

  12. Chandran V, Fronzes R, Duquerroy S, Cronin N, Navaza J, Waksman G. Structure of the outer membrane complex of a type IV secretion system. Nature. 2009;462:1011–5.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Liang Y, Aoyama T, Oka A. Structural characterization of the virB operon on the hairy-root-inducing plasmid A4. DNA Res. 1998;5:87–93.

    Article  CAS  PubMed  Google Scholar 

  14. Alt-Morbe J, Stryker JL, Fuqua C, Li PL, Farrand SK, Winans SC. The conjugal transfer system of Agrobacterium tumefaciens octopine-type Ti plasmids is closely related to the transfer system of an IncP plasmid and distantly related to Ti plasmid vir genes. J Bacteriol. 1996;178:4248–57.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Hofreuter D, Odenbreit S, Haas R. Natural transformation competence in Helicobacter pylori is mediated by the basic components of a type IV secretion system. Mol Microbiol. 2001;41:379–91.

    Article  CAS  PubMed  Google Scholar 

  16. Kumar RB, Xie YH, Das A. Subcellular localization of the Agrobacterium tumefaciens T-DNA transport pore proteins: VirB8 is essential for the assembly of the transport pore. Mol Microbiol. 2000;36:608–17.

    Article  CAS  PubMed  Google Scholar 

  17. Das A, Xie YH. The Agrobacterium T-DNA transport pore proteins VirB8, VirB9, and VirB10 interact with one another. J Bacteriol. 2000;182:758–63.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Dumler JS, Barbet AF, Bekker C, Dasch GA, Palmer GH, Ray SC, et al. Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and ‛HGE agentʼ as subjective synonyms of Ehrlichia phagocytophila. Int J Syst Evol Microbiol. 2001;51:2145–65.

    Article  CAS  PubMed  Google Scholar 

  19. Rikihisa Y. Role and function of the type IV secretion system in Anaplasma and Ehrlichia species. Curr Top Microbiol Immunol. 2017;413:297–321.

    CAS  PubMed  Google Scholar 

  20. Rikihisa Y, Lin M, Niu H. Type IV secretion in the obligatory intracellular bacterium Anaplasma phagocytophilum. Cell Microbiol. 2010;12:1213–21.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Christie PJ. Agrobacterium tumefaciens T-complex transport apparatus: a paradigm for a new family of multifunctional transporters in eubacteria. J Bacteriol. 1997;179:3085–94.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Ohashi N, Zhi N, Lin Q, Rikihisa Y. Characterization and transcriptional analysis of gene clusters for a type IV secretion machinery in human granulocytic and monocytic ehrlichiosis agents. Infect Immun. 2002;70:2128–38.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Kawahara M, Rikihisa Y, Lin Q, Isogai E, Tahara K, Itagaki A, et al. Novel genetic variants of Anaplasma phagocytophilum, Anaplasma bovis, Anaplasma centrale, and a novel Ehrlichia sp. in wild deer and ticks on two major islands in Japan. Appl Environ Microbiol. 2006;72:1102–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Barlough JE, Madigan JE, DeRock E, Bigornia L. Nested polymerase chain reaction for detection of Ehrlichia equi genomic DNA in horses and ticks (Ixodes pacificus). Vet Parasitol. 1996;63:319–29.

    Article  CAS  PubMed  Google Scholar 

  25. Torina A, Agnone A, Blanda V, Alongi A, DʼAgostino R, Caracappa S, et al. Development and validation of two PCR tests for the detection of and differentiation between Anaplasma ovis and Anaplasma marginale. Ticks Tick Borne Dis. 2012;3:283–7.

    Article  PubMed  Google Scholar 

  26. de la Fuente J, Atkinson MW, Naranjo V, de Mera IGF, Mangold AJ, Keating KA, et al. Sequence analysis of the msp4 gene of Anaplasma ovis strains. Vet Microbiol. 2007;119:375–81.

    Article  PubMed  CAS  Google Scholar 

  27. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248–54.

    Article  CAS  PubMed  Google Scholar 

  28. Glickman MH, Ciechanover A. The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction. Physiol Rev. 2002;82:373–428.

    Article  CAS  PubMed  Google Scholar 

  29. Schnell JD, Hicke L. Non-traditional functions of ubiquitin and ubiquitin-binding proteins. J Biol Chem. 2003;278:35857–60.

    Article  CAS  PubMed  Google Scholar 

  30. Welchman RL, Gordon C, Mayer RJ. Ubiquitin and ubiquitin-like proteins as multifunctional signals. Nat Rev Mol Cell Biol. 2005;6:599–609.

    Article  CAS  PubMed  Google Scholar 

  31. Appella E, Weber IT, Blasi F. Structure and function of epidermal growth factor-like regions in proteins. FEBS Lett. 1988;231:1–4.

    Article  CAS  PubMed  Google Scholar 

  32. Segura RL, Aguila-Arcos S, Ugarte-Uribe B, Vecino AJ, de la Cruz F, Goni FM, et al. The transmembrane domain of the T4SS coupling protein TrwB and its role in protein-protein interactions. Biochim Biophys Acta. 2013;1828:2015–25.

    Article  CAS  PubMed  Google Scholar 

  33. Schroder G, Lanka E. The mating pair formation system of conjugative plasmids—a versatile secretion machinery for transfer of proteins and DNA. Plasmid. 2005;54:1–25.

    Article  PubMed  CAS  Google Scholar 

  34. Davis CG. The many faces of epidermal growth factor repeats. New Biol. 1990;2:410–9.

    CAS  PubMed  Google Scholar 

  35. Schlieker C, Korbel GA, Kattenhorn LM, Ploegh HL. A deubiquitinating activity is conserved in the large tegument protein of the Herpesviridae. J Virol. 2005;79:15582–5.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Blasi F, Vassalli JD, Dano K. Urokinase-type plasminogen activator: proenzyme, receptor, and inhibitors. J Cell Biol. 1987;104:801–4.

    Article  CAS  PubMed  Google Scholar 

  37. Graf J, Ogle RC, Robey FA, Sasaki M, Martin GR, Yamada Y, et al. A pentapeptide from the laminin B1 chain mediates cell adhesion and binds the 67,000 laminin receptor. Biochemistry. 1987;26:6896–900.

    Article  CAS  PubMed  Google Scholar 

  38. Banks L, Pim D, Thomas M. Viruses and the 26S proteasome: hacking into destruction. Trends Biochem Sci. 2003;28:452–9.

    Article  CAS  PubMed  Google Scholar 

  39. Boutell C, Everett RD. The herpes simplex virus type 1 (HSV-1) regulatory protein ICP0 interacts with and Ubiquitinates p53. J Biol Chem. 2003;278:36596–602.

    Article  CAS  PubMed  Google Scholar 

  40. Everett RD, Meredith M, Orr A, Cross A, Kathoria M, Parkinson J. A novel ubiquitin-specific protease is dynamically associated with the PML nuclear domain and binds to a herpesvirus regulatory protein. EMBO J. 1997;16:566–77.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  41. Ovaa H, Kessler BM, Rolen U, Galardy PJ, Ploegh HL, Masucci MG. Activity-based ubiquitin-specific protease (USP) profiling of virus-infected and malignant human cells. Proc Natl Acad Sci USA. 2004;101:2253–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  42. Kattenhorn LM, Korbel GA, Kessler BM, Spooner E, Ploegh HL. A deubiquitinating enzyme encoded by HSV-1 belongs to a family of cysteine proteases that is conserved across the family Herpesviridae. Mol Cell. 2005;19:547–57.

    Article  CAS  PubMed  Google Scholar 

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Ms Xiaohong Zhang, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences is thanked for providing the required ticks for this research.


This study was financially supported by the National Key R&D Program of China (2017YFD0501200, 2018YFD0502305); NSFC (No. 31502054, No. 31502091); ASTIP (CAAS-ASTIP-2016-LVRI); NBCIS (CARS-37); 973 Program (2015CB150300); Jiangsu Co-innovation Center program for Prevention and Control of Important Animal Infectious Disease and Zoonosis.

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ZL and HY designed this study and critically revised the manuscript. MUM, NI, JY, SZ, QN, MR ZL, YZ, ZC and GG performed the experiments, data analysis, and drafted the manuscript. All authors read and approved the final manuscript.

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Correspondence to Zhijie Liu or Hong Yin.

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Mukhtar, M.U., Iqbal, N., Yang, J. et al. Identification and evaluation of UL36 protein from Dermacentor silvarum salivary gland and its interaction with Anaplasma ovis VirB10. Parasites Vectors 13, 105 (2020).

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