- Open Access
A tale of two communities: intestinal polyparasitism among Orang Asli and Malay communities in rural Terengganu, Malaysia
- Fatin Nur Elyana1,
- Hesham M. Al-Mekhlafi1, 2, 3Email author,
- Init Ithoi1Email author,
- Awatif M. Abdulsalam1,
- Salwa Dawaki1,
- Nabil A. Nasr1,
- Wahib M. Atroosh1,
- Mohamad Hafiz Abd-Basher1,
- Mona A. Al-Areeqi1,
- Hany Sady1,
- Lahvanya R. Subramaniam1,
- Tengku Shahrul Anuar4, 5,
- Yee Ling Lau1,
- Norhayati Moktar6 and
- Johari Surin1, 7
© The Author(s). 2016
- Received: 21 April 2016
- Accepted: 1 July 2016
- Published: 16 July 2016
Intestinal parasitic infections (IPIs) are still major health problems in many developing countries including Malaysia, particularly in the poor and socioeconomically deprived rural and remote communities in Peninsular Malaysia. This study was conducted to determine the prevalence of IPIs and to identify the key factors associated with intestinal polyparasitism as well as to evaluate the knowledge, attitude and practices (KAP) on IPIs among rural Orang Asli and Malay communities in Terengganu, Malaysia.
A cross-sectional study was conducted among 340 participants (165 Orang Asli and 175 Malay) aged ≤ 15 years from the Hulu Terengganu and Kemaman districts of Terengganu. Faecal samples were examined for the presence of intestinal parasites by using direct smear, formalin-ether sedimentation, trichrome stain, modified Ziehl Neelsen stain, in vitro cultivation in Jones’ medium, Kato Katz and Harada Mori techniques. Demographic, socioeconomic, environmental and behavioural information of the participants and their KAP for IPIs were collected by using a pre-tested questionnaire.
Overall, 149 (90.3 %) Orang Asli and 43 (24.6 %) Malay children were infected by at least one parasite species. The overall prevalences of intestinal polyparasitism among the Orang Asli and Malay were 68.5 % (113/165) and 14.3 % (25/175), respectively. Multiple logistic regression analysis showed that using unsafe water supply as a source for drinking water, the presence of domestic animals, not wearing shoes when outside, not washing vegetables before consumption, not washing hands after playing with soil, indiscriminate defecation and the low level of mother’s education were the key risk factors for intestinal polyparasitism among the Orang Asli, while working mothers and the presence of domestic animals were the risk factors among the Malay children. Almost all the Malays were well aware about the IPIs while Orang Asli respondents had a poor level of related awareness.
This study demonstrates that IPIs are highly prevalent in rural Terengganu, Malaysia. Community awareness about IPIs was found to be imperative in protecting Malay children from these infections. An integrated control programme for the prevention and control of IPIs is highly recommended for these communities, with a special emphasis on the Orang Asli population.
- Intestinal parasitic infections
- Neglected tropical diseases
- Soil-transmitted helminths
- Orang Asli
Intestinal parasitic infections (IPIs) are still major public health problems worldwide, particularly among children in poor and rural communities of most countries in tropical regions. It is estimated that about 2.0 billion people are infected with at least one intestinal parasite species while about 4.0 billion are at risk of infection [1, 2]. IPIs are caused by either helminths, such as Ascaris lumbricoides, Trichuris trichiura, hookworms, Strongyloides stercoralis and Taenia spp., or protozoan parasites, such as Entamoeba histolytica, Giardia duodenalis, Cryptosporidium spp., Cyclospora spp. and Blastocystis sp. [3, 4]. It is well documented that IPIs are associated with a complex web of causation that involves poverty (which is, in general, the root of these problems), inadequate sanitation, poor hygienic practices, illiteracy, ecosystem differences and overcrowding [5, 6].
Generally, IPIs occur silently as chronic infections and so the infected individuals are either asymptomatic or suffer from mild diseases; hence, these infections are considered as disablers rather than killers . When measured in disability-adjusted life years (DALYs), the most recent global estimates revealed that over 16 million DALYs are lost to IPIs (for instance, 8.37 million by cryptosporidiosis, 4.64 million by STH infections and 2.24 million by amoebiasis) [1, 2, 7]. Moreover, numerous studies reported that IPIs (mainly soil-transmitted helminths (STH) and giardiasis) during childhood are significantly associated with protein-energy malnutrition, iron deficiency anaemia (IDA), vitamin A deficiency (VAD), intellectual retardation and educational deficits that consequently lead to poor school attendance and poor educational achievement [8–13]. Moreover, the impact of these infections increased with the number of parasite species and intensity of infections [14, 15]. It was found that polyparasitism (the concurrent infection with multiple parasite species) is associated with higher mortality rates and may increase the sufferers’ susceptibility to other infections [15, 16]. In addition, the adverse consequences of these infections may continue into the adulthood with effects on the economic productivity and trapped the endemic populations in a cycle of poverty, underdevelopment and disease .
Despite the great socioeconomic and infrastructural development in Malaysia, several studies have demonstrated a high prevalence of IPIs with prominent morbidity in underprivileged communities, which is a trend that remains largely unchanged since the 1920s [18–25]. Unfortunately, data on IPIs among rural Malay population in Malaysia are not available and no study has yet addressed the status of these infections in Terengganu state, Malaysia. The present study aims to fill these gaps of information by investigating and comparing the prevalence, associated risk factors and people’s knowledge, attitude and practices (KAP) towards IPIs among the Orang Asli and the Malay populations in rural Terengganu, Malaysia. This information is important for a rationale design and implementation of effective control and prevention programmes against the IPIs among different rural communities in Malaysia.
Study design and study area
Terengganu state is situated in north-eastern Peninsular Malaysia and consists of eight districts. It has a population of about 1.0 million, with the Malays making up 94.7 % of the population, followed by the Chinese (2.6 %), with 49 % residing in rural areas . The Hulu Terengganu District (102.52°E, 5.01°N) is located near Kelantan and Pahang state borders, with a total area of 3874 km2 and a total population of 73,912, according to the population census 2010 . Likewise, the Kemaman district (103.26°E, 4.36°N) is located at the south of Terengganu state on the east coast of Malaysia facing the South China Sea, with a total area of 2536 km2 and a total population of 166,750. Generally, both districts, Hulu Terengganu and Kemaman, boast a tropical climate, with temperatures averaging from 26 °C to 32 °C and a mean humidity of 90 %. Rainfall is copious with an average of 2736 mm per year and the vegetation is the thick rainforest type and there are few streams in the area.
Study population and sample size
The main ethnic groups in Malaysia are Malays (54.1 %), Chinese (25.4 %) and Indians (7.5 %). Indigenous groups include the Orang Asli, Kadazans, Ibans and various other groups, all accounting for 11.7 % of the country’s total population (~28 million) . Orang Asli (a Malay term transliterated as ‘original people’) are the indigenous minority inhabitants of Peninsular Malaysia. The total population of Orang Asli is 178,197 throughout Peninsular Malaysia, comprising only 0.6 % of the total population in Malaysia . Despite intensive efforts by the government and private sectors to improve the quality of life of aboriginal communities throughout 59 years of independence (since 1957), little success has been achieved due to the adherence of the Orang Asli people to their jungle habitats. On the other hand, Malay communities are better in terms of environment cleanliness and housing conditions.
The minimum sample size required for this study was calculated according to the formula provided by Lwanga & Lemeshow . At a 5 % level of significance and a 95 % confidence level, the minimum number of participants required for the study was estimated at 276 (138 participants from each population), assuming that the prevalence of IPIs among Orang Asli children was about 90 % as previously reported [21, 25], and about 10 % among rural Malay population. However, at the villages, all children who were present during our visit were invited to participate in the study (universal sampling).
Overall, 443 eligible children have agreed voluntarily to participate in the study. However, the 340 children who delivered suitable samples for examination with complete questionnaire data were included in this study. Of these 340 participants, 165 children (77 girls and 88 boys) were from Orang Asli settlements (Senoi tribe) and 175 children (99 girls and 76 boys) were from Malay communities. Throughout many visits to the study areas, most of the Orang Asli children were observed playing outside without wearing shoes or slippers. Also, it was observed that poverty prevails in these areas with poor housing and living conditions. Better conditions in terms of housing and sanitary facilities and personal hygiene practices were observed in Malay rural communities. Some of the children play and swim in the streams/pools after school and in their leisure time.
A pre-tested questionnaire was used to collect the information on the demographic (age, gender and family size), socioeconomic (education attainment, occupation, household income), behavioural (personal hygiene such as hand washing, wearing shoes when going outside and consumption of raw fruits and vegetables), medical history (whether the participant has taken anthelminthic drugs, history of chronic diseases) and environmental factors (sanitation and living condition characteristics such as type of water supply, latrine system, garbage disposal system and presence of domestic animals) which were used to assess the potential risk factors of IPIs.
Moreover, the questionnaire includes questions on respondents’ KAP towards IPIs. Questions on the knowledge about symptoms, preventive measures and mode of transmission of IPIs were open-ended questions to avoid guessing which may give a false impression of the knowledge of the participant. However, the questions pertaining to the practices and daily activities were provided with multiple-choice answers to assess the frequency of doing these activities or actions. The children and their parents, who agreed voluntarily to participate, were interviewed face-to-face in their home settings by two research assistants from the Department of Parasitology, University of Malaya who were trained on how to administer the questionnaire for the purpose of this study.
Parasitological examination of faecal samples
The children were given a clearly labelled, wide mouth screw-cap containers and were instructed to bring their early morning stool samples the next day. An accurate demonstration of how to collect the stool samples was made to the children so as to avoid possible contamination in the course of collection at home. The containers were placed into zipped plastic bags, kept in a protected ice box and transported (within 8 h of collection) for examination at the Department of Parasitology, University of Malaya.
The examination was carried out by using different techniques, direct smear (wet mount) was applied on all samples and then formalin-ether sedimentation technique was used to increase the detection rates especially when the parasites are in few numbers . Moreover, Kato-Katz technique was used for egg counts to estimate the intensity of infections and the results were recorded as eggs per gram of stool (EPG) . Intensity of infection was graded as heavy, moderate, or light according to the criteria proposed by the World Health Organization . For Ascaris, Trichuris and hookworm infections, egg counts of ≥ 50,000 EPG, ≥ 10,000 EPG and ≥ 4000 EPG, respectively, are regarded as heavy infections while egg counts of 1–4999 EPG, 1–999 EPG and 1–1999 EPG, respectively, are regarded as light infections . In addition, Harada-Mori culture technique was applied to detect hookworm larvae in light infections .
A suitable amount (approximately 10 gm) of each faecal sample was mixed thoroughly and fixed in polyvinyl alcohol (PVA) for the detection of protozoans (Giardia, Entamoeba and Blastocystis) using trichrome staining technique [31, 32]. Moreover, faecal smears were prepared and stained with modified Ziehl-Neelsen stain, according to Henriksen & Pohlenz , for the detection of Cryptosporidium oocysts. In addition, approximately 50 mg of faeces were inoculated into a 15-ml screw-capped tube containing 5.0 ml of complete Jones’ medium and incubated at 37 °C . About 4.0 ml of the medium in each of these tubes was replaced with similar amount of the new complete Jone’s medium every alternate day starting from day 2 of cultivation. The presence of Blastocystis was observed by using light microscopy, daily for 14 days of cultivation. Different sizes of Blastocystis cells (2–15 μm) were seen as vacuolar (most common), granular, amoeboid (vacuolar or granular with pseudopodia) and cyst forms [34, 35]. Overall, the samples were considered as positive by the detection of eggs/larvae/cysts/trophozoites/oocysts using any of these seven techniques.
Data were double-entered by two different researchers into Microsoft Office Excel 2007 spreadsheets. Then, a third researcher cross-checked the two data sets for accuracy and created a single data set. Data analysis was made using IBM SPSS Statistics, version 18.0 (IBM Corporation, New York, NY, USA). Demographic, socioeconomic, environmental and behavioural characteristics as well as KAP variables were treated as categorical variables and presented as frequencies and percentages. Pearson’s Chi-square (χ 2 ) test or Fisher’s exact test were used where appropriate to examine the difference in proportions between groups and to test the association of polyparasitism prevalence as the dependent variable with the demographic (age, gender and family size), socioeconomic (parents’ educational and employment status, household monthly income, source of drinking water, presence of functioning toilet in the house, garbage disposal and presence of domestic animals in the households) and personal hygiene practices (washing hands before eating and after defecation, washing fruits and vegetables before consumption, wearing shoes when outside, eating soil (geophagy), boiling/filtering drinking water, cutting nails periodically and indiscriminate defecation) as the explanatory variables. All variables were coded in a binary manner as dummy variables, eg polyparasitism (positive = 1, negative = 0); gender (boys = 1, girls = 0), and so on. Odd ratios (OR) and 95 % confidence intervals (CI) were also computed. Likewise, to control the variation in number of children in households, weight cases, derived by the sampling fraction 1/number of participated children from each family, was used to analyse the KAP data.
In order to identify the risk factors significantly associated with intestinal polyparasitism (coded as 1 = polyparasitism, 0 = monoparasitism and uninfected), all variables that showed associations with P ≤ 0.25 in the univariate analyses were used to develop a multivariate logistic regression model as suggested by Bendel & Afifi . Moreover, sex variable was also included in the multivariable analysis as it has been considered as an important behavioural modifying factor . Population attributable risk fraction (PARF) was calculated for significantly associated risk factors . A P-value of < 0.05 was considered to be statistically significant.
The study protocol was approved by the Ethics Committee of the University Malaya Medical Centre (UMMC), Malaysia (Reference Number: 638.36). Permission was also obtained from the Department of Orang Asli Development (JAKOA), Ministry of Rural and Regional Development, Kuala Lumpur. At the study area, meetings were held with the heads of villages and parents to provide information about the objectives and protocol of the study and their permission to conduct the study was obtained. The children and their parents/guardians were informed that the procedures used did not pose any potential risk and their identities and personal data will be kept strictly confidential. They were informed that their participation was voluntarily and they could withdraw from the study at any time without citing any reason whatsoever. Written and signed or thumb-printed informed consent was obtained from parents or guardians on behalf of their children and these procedures were approved by the Medical Ethics Committee of the University of Malaya Medical Centre.
General characteristics of the households
General characteristics of children who participated in the study (n = 340)
No. of participants
Age groups (years)
Father’s education level
Never went to school
Mother’s education level
Never went to school
Low monthly household income (< RM500)
Large family size (≥7 members)
Piped water supply
Presence of toilet in house
With regard to Malay communities, almost all fathers and mothers were educated and had at least a primary education. Similarly, the majority of the families (80.6 %) had a household monthly income of > RM500. Moreover, 77.1 and 23.4 % of the children had working fathers and working mothers, respectively, who were employed as workers on oil palm plantations, farms, livestock preservation, government and private sectors. The houses were large and built of timber and concrete. All of the houses had toilet facilities, piped-in water and electricity.
Prevalence of IPIs
Prevalence of intestinal parasitic infections by parasite species and number of infections among Orang Asli and Malay in Terengganu (n = 340)
Orang Asli n (%)
Malay n (%)
Overall n (%)
Type of infection (n = 192)a
No. and type of polyparasitism (n = 138)b
Two parasite species
Three parasite species
Four parasite species
Five parasite species
With regard to the intensity of STH infections, the results showed that almost two-thirds (66.2 %) and one-third 39.3 % of the Trichuris and Ascaris infections, respectively, among Orang Asli, were of moderate-to-heavy intensity (mean EPG of ≥ 1000 for Trichuris and ≥ 5000 for Ascaris), whereas 17.9 % of the hookworm infections were of moderate intensity (mean EPG between 2000 and 3999). On the other hand, only 2 (11.8 %) and 1 (16.7 %) Malay children had moderate-to-heavy intensity Trichuris and Ascaris infections, respectively.
Risk factors of polyparasitism
Univariate analysis of factors associated with polyparasitism among Orang Asli and Malay children in Terengganu (n = 340)
OR (95 % CI)
OR (95 % CI)
School-age (≥ 6 years)
Preschool (< 6 years)
≥ 7 members (large)
< 7 members
Father’s educational level
Educated (≥ 6 years)
Mother’s educational level
Educated (≥ 6 years)
Father’s employment status
Mother’s employment status
Household monthly income
< RM 500
≥ RM 500
Presence of toilet in house
Source of drinking water
Unsafe source (river, rain)
Safe source (pipe)
Presence of domestic animals
Washing hands before eating
Washing hands after defecation
Washing hands after playing with soil
Eating soil habit (geophagy)
Cutting nails periodically
Wearing shoes when go outside
Washing fruits before eating
Washing vegetables before eating
Boiling/filtering water before drinking
With regards to Malay communities, Table 3 shows that polyparasitism was significantly lower among children of mothers who did not work compared with children of working mothers (10.4 vs 26.8 %; P = 0.009). Moreover, children from families with a low household monthly income (< RM500) (26.5 vs 11.3 %; P = 0.024) and those who have domestic animals in their households (18.8 vs 5.2 %; P = 0.015) had a higher prevalence rate compared to their counterparts.
Multivariate analysis of factors associated with polyparasitism among Orang Asli and Malay children in Terengganu (n = 340)
Orang Asli a-OR (95 % CI)
Malay a-OR (95 % CI)
Family size (≥ 7 members; large)
Mother’s educational level (< 6 years)
Father’s employment status
Mother’s employment status (not working)
Household monthly income (< RM 500)
Presence of toilet in house (no)
Source of drinking water (unsafe water)
Presence of domestic animals (yes)
Personal hygiene factors
Washing hands after playing with soil (no)
Washing vegetables before eating (no)
Wearing shoes when outside (no)
Indiscriminate defecation (yes)
Population attributable risk fraction (PARF) analysis showed that the number of polyparasitism cases among Orang Asli children could be reduced by 25.9, 22.1, 13.0 and 12.1 % if all children practiced good standards of personal hygiene, specifically by wearing shoes when going outside the house, using toilets for defecation (avoiding open defecation), washing their hands after playing in the soil and washing vegetables before consumption. Moreover, 23.1, 18.2 and 11.8 % of the polyparasitism cases could be avoided if there are no domestic animals at the households, the population had safe drinking water and the mothers had at least a primary education.
With regards to Malaya participants, three factors were confirmed by multiple logistic regression model analyses as the significant predictors of polyparasitism (Table 4). Children who have domestic animals at their household had 3.8 odds of having polyparasitism compared with those who do not have domestic animals. Similarly, children of mothers who do not work were found to have a lower risk of polyparasitism compared with children of working mothers (OR 0.34; 95 % CI: 0.12–0.95). Overall, PARF analysis showed that the number of polyparasitism cases could be reduced among the Malay children by 26.9 and 24.7 % when proper care is given to the children of working mothers and without domestic animals in the households, respectively.
Knowledge about IPIs, their signs and symptoms, transmission and prevention
Knowledge about IPIs symptoms, transmission and prevention among Orang Asli and Malay participants who had prior knowledge on IPIs (n = 160)
n = 104
n = 83
Heard about IPIs (n = 187)
Source of information
Do not remember
Signs and symptoms
Loss of appetite
Loss of weight
Do not know
Eating contaminated food
Eating raw/undercooked food
Drinking untreated water
Playing with soil
Not cutting nails regularly
Do not know
Taking deworming drugs
Washing hands before eating
Wearing shoes when outside the house
Washing vegetables & fruits before eating
Boiling/filtering drinking water
Do not know
In general, 96.3, 97.6 and 98.8 % of the Malay respondents were able to mention at least one symptom of IPIs, one mode of transmission and one preventive measure against IPIs, respectively, compared to 75.6, 52.6 and 64.1 % of the Orang Asli respondents (P < 0.001). The percentages of respondents who mentioned diarrhoea (42.7 vs 11.5 %; P < 0.001) and loss of weight (17.1 vs 5.2 %; P = 0.018) were significantly higher among the Malay respondents, while the percentage of those who mentioned itching was significantly higher among the Orang Asli compared to the Malay (25.6 vs 9.8 %; P = 0.008).
More than half of the Malay respondents had knowledge about the role of contaminated hands and contaminated food in transmitting IPIs compared to less than one-quarter of their Orang Asli counterparts (P < 0.001). Interestingly, eating raw and/or undercooked food was mentioned by only four Malay respondents (4.9 vs 0 %; P = 0.047). However, a double percentage of the Orang Asli mentioned playing with the soil as a mode of transmission compared with the Malay (24.4 vs 12.2 %; P = 0.046). In line with these results, 54.9 % (45/82) and 43.2 % (35/82) of the Malay respondents mentioned washing their hand before eating and washing vegetables and fruits before consumption as preventive measures against IPIs compared to 17.9 % (14/78) and 19.2 % (15/78) of their Orang Asli counterparts (P < 0.001).
Attitude and practices towards IPIs
Attitude and perceived practices towards intestinal helminths among Orang Asli and Malay participants in Terengganu, Malaysia
Orang Asli (n = 104)
Malay (n = 83)
Attitude (n = 160)
Effects of intestinal parasitic infections
Harmful to peoples’ health
Not harmful to peoples’ health
Do not know
Faeces as source of infections
Do not know
Practices (n = 187)
Washing hands before eating
Washing hands after defecation
Washing hands after playing with soil
Washing vegetables before consumption
Washing fruits before consumption
Using safe sources for drinking water
Wearing shoes when outside the house
Boiling/filtering drinking water
Using toilets for defecation
Cutting fingernails regularly
Eating soil (geophagy)
Treatment-seeking behaviour for GIT symptoms
Go to clinic as a first line activity
Go to traditional healer as a first line activity
Use herbal remedies as a first line activity
Regarding the hygiene practices, the results revealed that the Malay respondents had significantly higher levels of personal hygiene practices such as washing hands before having meals and after playing in the soil, wearing shoes when outside the house, washing vegetables/fruits before consumption and boiling/filtering drinking water compared with their Orang Asli counterparts (P < 0.001). In terms of treatment-seeking behaviour, going to the nearest clinic for treatment in case of diarrhoea and abdominal pain as a first line activity was significantly higher among the Malay than Orang Asli respondents (81.9 vs 55.8 %; P < 0.001), while significantly higher percentages of the Orang Asli tended to do nothing (17.3 vs 1.2 %; P < 0.001) for such symptoms compared to their Malay counterparts.
To the best of our knowledge, this is the first study to provide information on the epidemiology of IPIs in Terengganu state and to compare the IPIs status between the Orang Asli and Malay populations residing the same rural areas. Our findings show that 90.3 % of the Orang Asli children were found to be positive for at least one intestinal parasite species, with trichuriasis being the predominant infection. These findings are consistent with several previous studies among the Orang Asli communities in other states such as Pahang, Perak, Selangor and Kelantan [18, 21, 23, 25, 39]. Hence, this could be attributed to the same epidemiological characteristics of the Orang Asli throughout West Malaysia. These include the poor housing conditions with the lack of access to safe drinking water and adequate sanitation, contaminated environment, high illiteracy rate and unhygienic practices by these people .
For protozoan infections, the present study showed that about one-third (34.5 %) of the Orang Asli children were infected with Blastocystis sp., while the prevalence of Entamoeba histolytica/dispar/moshkovskii (Entamoeba spp.) and G. duodenalis was 14.5 % for both. Previous studies conducted among the Orang Asli children in different states showed a varying prevalence of infections with Blastocystis sp. ranging between 4.4 and 52.3 % [32, 34, 40], Giardia ranging between 0.2 and 29.2 % [13, 24] and Entamoeba spp. ranging between 1.0 and 61.0 % . Although the role of Blastocystis sp. in gastrointestinal disease is still controversial, there is an increasing body of evidence suggesting that Blastocystis is pathogenic, and its pathogenicity was found to be related to specific subtypes, parasite burden and host’s immune status [42–44]. Moreover, many reports from Malaysia have claimed a pathogenic role of Blastocystis and clearly demonstrated that genotypes of Blastocystis can cloud parasite pathogenicity, particularly subtype 3 [45–48]. Hence, it seems currently wise to consider Blastocystis sp. among the intestinal parasites reported by the present study.
With regard to the Malay children, 24.6 % of them were found to be infected with at least one parasite species, with blastocystosis being the predominant IPIs (17.7 %), followed by trichuriasis (9.7 %) and giardiasis (8.6 %). Overall, the prevalence of STH infections was significantly lower in Malay children compared to their Orang Asli peers. This could be explained by the better housing and environmental conditions as well as the better knowledge and hygienic practices among Malay communities compared to the Orang Asli communities. Likewise, earlier studies among Malays in urban areas in Selangor state in 1984 and 1994 showed a very high rate of IPIs: 86.3 and 59.4 %, respectively [49, 50]. However, a significant reduction in the prevalence of STH infections in the urban areas was reported and this could be explained by the great socioeconomic and infrastructural development in urban Malaysia during the past three decades [51, 52]. Overall, our findings are consistent with results of previous studies from other countries that revealed a significantly higher prevalence of IPIs among indigenous communities compared with non-indigenous groups [53, 54].
Our findings showed that the majority of the infected children had polyparasitism (71.9 %), with 34.1, 41.3 and 21.7 % of the polyparasitism being concurrent infections with two, three and four parasite species, respectively. Previous studies in other countries, including the Philippines , Côte d’Ivoire , Kenya , Rwanda  and Cameroon  showed that polyparasitism was found to be the norm among the populations studied. Among the infected children, the present study disclosed a significantly higher prevalence of polyparasitism among the Orang Asli children (75.8 %) compared to Malays (58.1 %) in Terengganu. The only study on the polyparasitism among Orang Asli children was conducted in Pahang state and reported that 71.4 % of the children studied had polyparasitism . Likewise, previous studies among the Orang Asli indicated that the majority of infected children were hosted two or more parasite species, with T. trichiura and A. lumbricoides representing the common mixed infection [19, 20, 22, 52]. A similar situation was reported in different aboriginal communities in other countries [54, 60, 61].
The present study investigated the risk factors associated with intestinal polyparasitism among the studied children and revealed different scenarios in each population. In agreement with our findings, recent studies among the Orang Asli communities reported a significantly higher prevalence of STH [20, 22], Giardia , Entamoeba spp.  and Blastocystis sp. [32, 34] infections among individuals who used unsafe sources for drinking water. Orang Asli prefer to live close to water streams and use water from these streams for most of their daily activities, including swimming, cooking, drinking, bathing and washing. Moreover, rivers are also their preferred sites for defecation, especially for children [25, 62]. This practice of indiscriminate defecation near the streams, around the houses, playgrounds and roadsides by the residents increases the contamination of the environment, thereby increasing the occurrences of infection and re-infection with IPIs. Moreover, the contamination of rivers with intestinal parasites was proven by previous reports conducted in Malaysia . In Malaysia, the government has made intensive efforts to improve the quality of life of the indigenous people throughout the country, with their main strategy being to reallocate those living in remote areas to new settlements at the periphery of towns. However, the adherence of the Orang Asli people in Peninsular Malaysia to their jungle habitats has constrained these efforts. As an alternative, hundreds of houses were built or restored for the Orang Asli people in their remote areas . Unfortunately, we observed that the toilets were used as storage rooms due to cultural beliefs that toilets should not be located inside the house and the lack of knowledge about the impact of sanitation.
Interestingly, the present study identified the presence of domestic animals in households as a significant risk factor of polyparasitism among both communities, Orang Asli and Malay. Previous studies in Malaysia reported a significant association between the presence of domestic animals and STH , Giardia , Entamoeba spp.  and Blastocystis sp.  infections. We found that domestic animals at the studied communities usually do not undergo deworming and therefore they are possible carriers of many parasites. The role of stray and domestic animals as reservoirs for zoonotic diseases has been known as a significant health problem worldwide [66, 67]. In the same vein, recent studies in rural and urban areas of different states of Malaysia revealed an extraordinary high level of soil contamination with STH eggs and highlighted the involvement of stray and domestic cats and dogs as sources of contamination [68, 69]. Besides, a wide range of animal parasites such as Ancylostoma ceylanicum, Ancylostoma caninum, Toxocara spp., Dipylidium caninum, Spirometra spp., Entamoeba spp., G. duodenalis, Cryptosporidium spp., Balantidium coli and Isospora spp. were recovered in soil samples as well as dogs and cats faecal samples [68–71]. Similar findings have been reported in many previous reports from different developed and developing countries [72–76].
Eggs and larvae of STH, as well as cysts and oocysts of protozoans, can remain viable and infective in the environment for a very long period of time; hence, involvement of human and animals in a mechanical transmission of intestinal parasites can also be postulated. Infective stages present in the soil could easily get picked-up by the fur of animals (cats and dogs) and then not washing hands after handling or playing with these animals could facilitate the spread of the infections. Similarly, not wearing shoes when outside the house may result in skin penetration by hookworm larvae or contribute to the contamination of houses with eggs/cysts/oocyst of other parasites and this was also identified by the present study as a significant predictor of polyparasitism among the Orang Asli children. This is in agreement with a previous report on polyparasitism among these children in Pahang state and other studies on STH and Giardia in Peninsular Malaysia [20, 24, 25].
In addition, Malay children of working mothers had a significantly higher prevalence of polyparasitism compared to their peers and this is in agreement with previous studies in Thailand , India  and Mexico . The absence of mothers during the daytime causes the loss of many child healthcare and hygiene activities provided by the mothers and creates more opportunities for young children to get exposed and infected as they play outdoors. Similarly, we found that the Orang Asli children born to non-educated mothers had a significantly higher prevalence of polyparasitism compared to those of educated mothers and this is consistent with recent studies among Orang Asli in Malaysia [23, 25] and other countries [80–82]. Non-educated mothers would probably have less health-related knowledge and concern attitude, thus give less health guidance to their children.
Our findings also revealed that not washing vegetables prior to consumption was a significant risk factor of polyparasitism among the Orang Asli children. Vegetables could be the medium of transmission in cases where the surface carries parasite infective stages, especially when the vegetables have been in contact with the contaminated ground or due to contamination at the source by fertilisation with night soil. This finding is consistent with previous studies in Orang Asli communities [23–25] and other countries [83, 84]. A high level of parasitic contamination of raw vegetables was reported in Iran , Egypt , Saudi Arabia  and Zanzibar .
With regards to the respondents’ KAP about IPIs, the present study showed distinct situations in both communities, with almost all the Malays having had heard about IPIs and having prior knowledge about IPIs signs and symptoms, ways of transmission and prevention. In contrast, although 75 % of the Orang Asli respondents had heard about IPIs, only 75.6, 52.6 and 64.1 % of them had knowledge on IPIs signs and symptoms, ways of transmission, and prevention, respectively. A similar level of knowledge was reported in Malaysia and abroad [22, 89].
Orang Asli settlements in Terengganu are located close to the Malay villages; that said, the environment’s soil enrichment is not a determining factor for the parasite thriving, but the human factor is. The better knowledge and attitude among the Malays translated into significantly higher levels of hygienic practices among this population compared to Orang Asli. Hence, we believe that the high level of awareness pertaining to the IPIs is the essential factor that explains the distinct IPIs situation in both populations. At the same time, we cannot ignore the critical role of proper sanitation that helps break helminth transmission cycles and is considered a key component in any intestinal parasite control programme. It was concluded that awareness by itself might be not enough to protect these people from infection as the lack of access to safe drinking water and adequate sanitation are the driving forces behind the risk behaviour of individual community members . Likewise, chemotherapy for the treatment of IPIs in highly endemic areas does not ensure protection against infection and has not had long-lasting success. For instance, previous studies from Malaysia and other countries have reported rapid reinfection of different IPIs including STH, schistosomiasis and protozoan infections. Within 6 months of complete deworming, the prevalence rate and intensity of infection were shown to return to the pre-treatment levels [62, 91]. Overall, Orang Asli communities in rural Peninsular Malaysia have almost similar socioeconomic, environmental and health profiles. This is also applicable to rural Malay communities. Thus, we may speculate that the findings can be generalised to other rural Orang Asli and Malay children in other states. However, further studies are required to confirm this hypothesis.
Limitations of the study
Some limitations should be borne in mind when interpreting the findings of the present study. First, a cross-sectional design was used to gather results of the study and this limits our ability to confirm the causal relationship between IPIs and the identified risk factors. Secondly, this study had to rely on a single faecal sample instead of the ideal three consecutive samples because of the limitation of resources and the cultural belief of the Orang Asli against giving their faecal samples. Thus, the prevalence rate of IPIs is likely to be underestimated due to the temporal variation in egg excretion and cyst/oocysts shedding over hours and days . Moreover, we used a single Kato-Katz smear instead of the multiple Kato-Katz smear examination that is reported to enhance the sensitivity of helminth diagnosis . However, we used seven different methods to examine the faecal samples and this could help to overcome this limitation. Thirdly, the diagnostic techniques used in our study may have a low sensitivity for the detection of certain parasite species (eg Strongyloides stercoralis). This could also be due to the storage of faecal samples in cold temperature (4–6 °C), which was found to interfere with the parasitological diagnosis of strongyloidiasis .
The findings of the present study show that IPIs are highly prevalent and are major public health concerns among rural communities in Terengganu state, with 68.5 % (113/165) of the Orang Asli children having polyparasitism compared to 14.3 % (25/175) of their Malay peers who reside the same areas. The presence of domestic animals was identified as a significant risk factor of polyparasitism in both populations, suggesting the potential role of animals in the epidemiological cycle of IPIs in these communities. Moreover, our findings clearly demonstrated a bigger web of causation of polyparasitism (including a subset of unhygienic practices, lack of access to safe drinking water and mothers’ low education) among the Orang Asli communities than among the Malays.
In addition, all Malay participants had high levels of knowledge and attitude about IPIs compared to poor levels among their Orang Asli peers. Hence, there is a great need to implement an innovative and integrated control programme to reduce the prevalence of these infections significantly and to save these children from the possible negative impacts of IPIs as a part of the efforts to improve the quality of life of rural population. A proper health education regarding good personal hygiene practices, providing proper and adequate sanitation and safe water supply should be considered in the control programme targeting the Orang Asli population.
EPG, eggs per gram of stool; IPIs, Intestinal parasitic infections; KAP, knowledge, attitude and practices; STH, soil-transmitted helminths
The authors would like to acknowledge the Department of Orang Asli Development (JAKOA), Ministry of Rural and Regional Development, Kuala Lumpur, Malaysia for their generous support during this study. Thanks also are indebted to the parents and their children for their participation in this study. The authors are also very thankful to Mr. Abdulaziz Ali Alkazhi from University of Malaya for his fruitful help during field work.
This study was funded by the University of Malaya High Impact Research Grant UM-MOHE (UM.C/625/1/HIR/MOHE/MED/16) from the Ministry of Higher Education Malaysia, and also by the University of Malaya Research Grants; RG510-13HTM and RG331-15AFR. The funding bodies have no role in the study and publication process.
Availability of data and material
The datasets supporting the conclusions of this article are included within the article. Raw data are available from the authors upon request.
HMA, II and YLL designed and supervised the study. FNE, NAN and WMA conducted the sampling and field survey. HMA analysed the data. HMA, FNE and II wrote the paper. AMA, NAN, WMA, HS, SD, MAA, MHA and LRS were involved in the laboratory examination of samples. JS, NM and TSA revised the manuscript. All authors approved the final version of the manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
The study was approved by the Medical Ethics Committee of the University of Malaya Medical Centre, Kuala Lumpur (Reference Number: 638.36). All participants gave a written signed or thumb-printed consent to take part in the study.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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